Reusing intravaginal progesterone releasing devices for oestrous synchoronization in ewes

In this study, the second use of an intravaginal progesterone-releasing device or controlled intravaginal drug release device (CIDR) was evaluated. After a first use of 11 days, the CIDR was again used for either nine or 12 days with 200 or 300 IU equine chorionic gonadotrophin (eCG) being injected on its removal. Sixty-four ewes were randomly distributed to four treatments (n=16/group): CIDR9+eCG200, CIDR9+eCG300, CIDR12+eCG200, and CIDR12+eCG300. The eCG was administered intramuscularly on withdrawal of the device. Thus, the experiment was a completely randomized design with a 2×2 factorial arrangement of treatments. Oestrus presentation did not differ between treatments (P =0.29). However, with the dose of 200 IU of eCG, oestrus presentation tended to increase (P =0.08). The onset and duration of oestrus, percentage of gestation, and return to oestrus did not differ between treatments (P >0.05). Progesterone concentration in serum was greater (P < 0.05) in ewes treated with CIDR12+eCG300. Prolificacy was greatest (1.44) with the CIDR12+eCG300 treatment and was different (P = 0.001) from the treatments CIDR9+eCG200 (1.21) and CIDR9+eCG300 (1.20), but not from the CIDR12+eCG200 treated ewes (1.31). The CIDR12+eCG300 treatement produced the highest percentage of twin births (45.8%) (P =0.001). Leaving the device in place for 12 days increased (P =0.001) the incidence of twin births. Use of the CIDR for a second time synchronized oestrus in ewes successfully with better fertility being obtained when the device was left in place for 12 days, and 300 IU of eCG was injected on its removal. Key words: gonadotropin, progesterone device, synchronization

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Comparison between Single and Double Injection of Pituitary Gland (PG) on the Breeding Performance of Climbing Perch, Anabas Testudineus (Bloch)

Journal of Bio-Science ◽

10.3329/jbs.v14i0.443 ◽

1970 ◽

Vol 14 ◽

pp. 57-60

Author(s):

Shahid Uddin Mahmood

Keyword(s):

Pituitary Gland ◽

Key Words ◽

Single Injection ◽

Breeding Performance ◽

Female Fish ◽

Anabas Testudineus ◽

Double Injection ◽

Climbing Perch ◽

Randomized Design ◽

Completely Randomized Design

An investigation was carried out to study the effects of single and double injection of given dose of pituitary gland (PG) on the breeding performance viz., ovulation response, fertilization, and hatching of climbing perch, Anabas testudineus was tested. Pituitary gland dose of 1.2 mg/100g was used in this experiment. Twenty female fish was arranged into two treatments: T1 and T2, each with 10 replications. In T1, the entire dose was administered in a single injection and in T2 the same dose was given in two equal injections three hr apart. The experiment was laid in completely randomized design (CRD). It was found that administration of PG in split doses (double injection) had no additional advantages (ovulation 100%, fertilization 81.56% and hatching 72.68%) over the single injection (ovulation 100%, fertilization 83.03% and hatching 75.48%) treatment. Similarly, the ovulation time of the fish did not vary significantly in either single or double injection of PG (single injection 7.12 hr; double injection 7.20 hr). The results demonstrated that splitting of pituitary gland dose (double injection) has no additional advantages on breeding performance of A. testudineus. Therefore, single injection may be preferred over the second injection for breeding of this fish. Key words: Breeding, pituitary gland (PG), fertilization, hatching J. bio-sci. 14: 57-60, 2006

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The Insecticide Toxicity and Repelling Ability of Jeruk Purut (Citrus hystrix D.C) Leaf Extract on Maize Weevil (Sitophilus zeamais Motsch)

JURNAL AGRONIDA ◽

10.30997/jag.v5i1.1849 ◽

2019 ◽

Vol 5 (1) ◽

Author(s):

Alfia Wulansari ◽

Nur Rochman ◽

Setyono Setyono

Keyword(s):

Mortality Rate ◽

Key Words ◽

Leaf Extract ◽

Insect Pest ◽

Sitophilus Zeamais ◽

Maize Weevil ◽

Insecticide Toxicity ◽

Randomized Design ◽

Completely Randomized Design ◽

Sitophilus Zeamais Motsch

Sitophilus zeamais Motsch. is a primary storage insect pest damaging maize grains. Control of S. zeamais can be done by applying biopesticide. This study was aimed at assessing the ability of Jeruk Purut leaf extract, as biopesticide, to kill and repel Sitophilus zeamais Motsch. The study was conducted at Entomology Laboratory, Seameo Biotrop in March to August 2018. A completely randomized design with three replicates in each rate of Jeruk Purut leaf extract was used. In preliminary pesticide toxicity experiment, five rates of Jeruk Purut leaf extract, namely 0%, 2%, 4%, 6%, and 8% were applied. In preliminary repelling ability experiment, five rates of Jeruk Purut leaf extract, namely 1%, 2%, 4%, 6%, and 8% were applied. In the main pesticide toxicity and repelling ability trial, five rates of Jeruk Purut leaf extract, namely 3%, 6%, 9%, 12%, and 15% were applied. Results showed that in 24 hour after treating (HAT) no significant effects were found. Significant effects of Jeruk Purut leaf extract as insecticide on S. zeamais were found in 48 and 72 HAT. The highest mortality rate (82.2%) of S. zeamais was found in 15% rate of Jeruk Purut leaf extract in 72 HAT. The application of Jeruk Purut leaf extract was not found to give significant effects on repelling S. zeamais in 24, 48, and 72 HAT as indicated in their sharply fluctuating repellent values. The highest repelling ability (95%) was found in the application of 9% Jeruk Purut leaf extract in 24 HAT. It was concluded that Jeruk Purut leaf extract was more potential as insecticide repellent for Sitophilus zeamais Motsch. Key words: biopesticide, repellent, insecticide, maize, primary storage insect pest

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Isolasi dan Uji Patogenitas Isolat Fungi Entomopatogen Terhadap Stadium Dewasa Nyamuk Aedes aegypti

JURNAL BIOLOGI PAPUA ◽

10.31957/jbp.832 ◽

2019 ◽

Vol 11 (1) ◽

pp. 33-41

Author(s):

Supiyanto Supiyanto ◽

Emantis Rosa ◽

Bambang Irawan ◽

Nismah Nukmal

Keyword(s):

Aedes Aegypti ◽

Key Words ◽

Entomopathogenic Fungi ◽

Microbiology Laboratory ◽

Penicillium Sp ◽

Randomized Design ◽

Mosquito Mortality ◽

Completely Randomized Design ◽

Aspergillus Sp

This research aims to determine the effectiveness of four types of entomopathogenic fungi isolated from Ae. aegypti mosquitoes from Bandar Lampung to the mortality of adult stages of Ae. aegypti mosquitoes. This research was conducted in October 2018-January 2019 at the Microbiology Laboratory of FMIPA, University of Lampung. This research using factorial completely randomized design with two factor treatment. The first factor is type of isolate (Mucor sp., Penicillium sp., IL3 (unidentified), Aspergillus sp.) and second faktor is dilution (control, 10 (without dilution), 10-1, 10-2, 10-3). Data were analyzed using ANOVA. If there are significant differences, then it is continued by the Duncan Test at the level of 5%. The results showed that the four types of fungi (Mucor sp., Penicillium sp., Aspergillus sp. and IL3) were able to cause mortality of Ae. aegypti mosquitoes with the highest killing power was in Mucor sp. 10 (without dilution) is 43.33%. But the most effective is Mucor sp. 10-3 because the highest dilution has been able to cause mosquito mortality by 30%. Key words: Ae. aegypti, DHF, entomopathogenic fungi, isolate, dilution.

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PENGARUH PENGGUNAAN KONSENTRAT DALAM PAKAN RUMPUT BENGGALA (Panicum maximum ) TERHADAP KECERNAAN BAHAN KERING DAN BAHAN ORGANIK PADA KAMBING LOKAL

ZOOTEC ◽

10.35792/zot.34.0.2014.4798 ◽

2014 ◽

Vol 34 ◽

pp. 108

Author(s):

Jems Momot ◽

Kartini Maaruf ◽

M R Waani ◽

Ch J Pontoh

Keyword(s):

Organic Matter ◽

Key Words ◽

Dry Matter ◽

Panicum Maximum ◽

Randomized Design ◽

Guinea Grass ◽

Completely Randomized Design

A research was conducted to evaluate the effects of concentrate utilization in Guinea grass (Panicum maximum) ration on dry matter and organic matter digestibilities. Twelve male local goats were used in this experiment and located in individual pens. Completely Randomized Design (CRD) with four treatments and three replicates was used. The treatments were R1 = 100 % guinea grass, R2 = 75 % guinea grass + 25 % concentrate, , R3 = 50 % guinea grass + 50 % concentrate, and R2 = 25 % guinea grass + 75 % concentrate. The results showed that effects on dry matter and organic matter digestibilities were high significantly different among treatments (P < 0.01). Ration without concentrate was significantly lower compared to ration with concentrate;. It can be concluded that the best dry matter and organic matter digestibilities were on 75 % concentrate in the ration Key words : Guinea grass, concentrate, digestibility, dry matter, organic matter, and local goats

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Pengaruh Temperatur dan Lama Thawing terhadap Kualitas Spermatozoa Sapi dalam Penyimpanan Straw Beku

Sains Peternakan ◽

10.20961/sainspet.v8i1.4740 ◽

2010 ◽

Vol 8 (1) ◽

pp. 22

Author(s):

S Utomo ◽

E Boquifai

Keyword(s):

Key Words ◽

The Other ◽

Effect Of Temperature ◽

Randomized Design ◽

Frozen Semen ◽

Completely Randomized Design ◽

Motility Of Sperm ◽

Better Than

This research was conducted to investigate the effect of temperature and duration of thawing frozen semen in ministraw on quality sperm. Seventy five ministraws frozen semen of Simmental breed were thawing by water with temperature 5, 26 and 37 °C, with duration of 5, 10, 15, 20 and 30 second. The quality sperm post thawing was measured based on pH and motility. The design of this research was completely randomized design with factorial clasification 3 x 5. The result showed that the motility of sperm was thawing 37°C better than the other temperature. The quality of sperm was no affected by temperature and duration of thawing. It could be concluded that thawing of frozen semen will have better done at 37°C with duration of 15 second.Key words : Thawing, sperm, ministraw, frozen semen.

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Studi Awal Regenerasi Sowang (Xanthostemon novaguineense Valet.)

JURNAL BIOLOGI PAPUA ◽

10.31957/jbp.106 ◽

2018 ◽

Vol 9 (1) ◽

pp. 31-36

Author(s):

Sri Wilujeng ◽

Verena Agustini

Keyword(s):

Plant Regeneration ◽

Seed Germination ◽

Key Words ◽

Human Activities ◽

Land Conversion ◽

Endemic Plant ◽

Randomized Design ◽

Chemical Soil ◽

Completely Randomized Design ◽

Set Up

Sowang (Xanthostemon novaguineense Valet.) is an endemic plant of Cycloops and strictly distributed. Since years, the population of the plants is threatened by human activities through land conversion, forest burning and logging. There are only few seedling grow in Cycloops naturally. Sowang wood is known as a good quality, because of its inherent hardness and high density. Scienfific information about this species is remain unclear. Regarding the plant regeneration, it is also not enough information so far. The study was conducted at three places around Cycloops areas namely Waena, Doyo Baru (sowing habitat) dan Wambena (no sowang areas) of Jayapura Regency, Papua and also greenhouse of FMIPA Cenderawasih University. The experimental units were set up in a completely randomized design with 3 treatments. Each experiment was carried out in three replicated and each replicate includes 100 seeds. Data were analyzed by analysis of variance (ANOVA), followed by LSD α=0.05. The result showed that there is no relation between geographical distribution of Sowang at Cycloops and the phisical and chemical soil. All type of soil used in this study was good as media of the seed germination. Regarding the ratio of the sapling population, the germination is still low only 18%, the seedling only survive for 38 days after sowing. Key words: Cycloops, regeneration, Sowang, Papua.

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PENGARUH PENGGANTIAN SEBAGIAN RANSUM DENGAN TEPUNG LIMBAH LABU KUNING (Cucurbita moschata) TERHADAP PERFORMANS AYAM PEDAGING

ZOOTEC ◽

10.35792/zot.39.2.2019.25528 ◽

2019 ◽

Vol 39 (2) ◽

pp. 345

Author(s):

Julin Salauhiang ◽

Marie Najoan ◽

Cherly J. Pontoh ◽

Meity R. Imbar

Keyword(s):

Body Weight ◽

Key Words ◽

Partial Replacement ◽

Cucurbita Moschata ◽

Broiler Performance ◽

Randomized Design ◽

Completely Randomized Design ◽

Average Body ◽

Basal Ration ◽

Average Body Weight

EFFECT OF REPLACEMENT SOME RATIONS WITH FLOUR YELLOW PUMPKIN WASTE (CUCURBITA MOSCHATA) AGAINST PERFORMANCE BROILER. This study aims to determine the extent of the partial replacement of rations with pumpkin waste flour (Cucurbita moschata) as measured by broiler performance. Using 100 unsexed broiler Cobb 707 aged 1 day with average body weight ± 45.32 g. The design used was a Completely Randomized Design (CRD) consisting of 4 treatments and 5 replications. The treatment composition is as follows: R0 = 100% basal ration (RB) + 0% pumpkin waste flour (TLLK), R1 = 95% RB + 5% TLLK, R2 = 90% RB + 10% TLLK, R3 = 85% RB + 15% TLLK. Results of analysis of variance showed that the dissecting treatment was not significant (P>0.05) for the consumption, weight and conversion of ration The use of pumpkin waste flour can replace a portion of the ration up to 15%.Key words: Performance, waste pumpkin, broiler.

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Pengaruh Penambahan Crude Tannin pada Sperma Cair Kambing Peranakan Ettawa yang Disimpan Selama 14 Hari terhadap Viabilitas Spermatozoa (The Effect of Crude Tannin Addition to Liquid Semen of Ettawa Crossbred Goat on The Viability of Spermatozoa During 14

Buletin Peternakan ◽

10.21059/buletinpeternak.v34i1.100 ◽

2012 ◽

Vol 34 (1) ◽

pp. 1

Author(s):

Oktora Dwi Putranti ◽

Kustono (Kustono) ◽

Ismaya (Ismaya)

Keyword(s):

Key Words ◽

Analysis Of Variance ◽

Sperm Quality ◽

Test Procedure ◽

Quality Characteristics ◽

Quality Data ◽

Randomized Design ◽

Completely Randomized Design

This study was aimed to obtain viability of Ettawa Crossbred spermatozoa into day 14th on with the addition of crude tannin (CT). The design used was one-way completely randomized design with five replications and five treatments. The data of value identified sperm were analyzed descriptively. Sperm quality data were analyzed using one way analysis of variance (ANOVA) and Tukey-W-test procedure. The results showed that the sperm quality characteristics were as the followed: volume 1.4+0.42 ml; pH 6.84+0.27; creamy milk color; consistency thick; motility ++ and concentration (328.8+80.74) x 107/ml semen. Spermatozoa viability were K0 (control) 29.89+16.52%; K1 (CT 2.5%) 65.33+8.95%; K2 (CT 5%) 58.20+12.4%; K3 (CT 10%) 52.61+15.34%; and K4 (CT 20%) 47.84+13.84%. It was concluded that the addition of 2.5% crude tannin gave significant effect on viability of Ettawa Crossbred spermatozoa during 14 days storage, whereas addition of 20% crude tannin had caused low percentage of live spermatozoa.(Key words: Spermatozoa, Crude tannin, Viability)

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MATURASI LELE MUTIARA Clarias gariepinus BETINA MELALUI PENAMBAHAN HORMON OODEV DAN ESTRADIOL-17ß PADA PAKAN

Jurnal Akuakultur Rawa Indonesia ◽

10.36706/jari.v9i1.13075 ◽

2021 ◽

Vol 9 (1) ◽

pp. 30-40

Author(s):

Oktavia Rugus Ayu Enditha ◽

Deny Sapto Chondro Utomo ◽

Munti Sarida

Keyword(s):

Key Words ◽

Reproductive Cycle ◽

Clarias Gariepinus ◽

Spawning Season ◽

Maturity Index ◽

African Catfish ◽

North African ◽

Gonadal Maturity ◽

Randomized Design ◽

Completely Randomized Design

The problem in Mutiara strain of North African catfish Clarias gariepinus culture is that the production of both larvae and seeds is still inhibited by the seasonal reproductive cycle where the catfish only spawn once during the spawning season. The purposes of this study were to evaluate the effect of administration of oodev and estradiol-17ß on the maturity process and to determine the best doses in accelerating the maturion of female Mutiara strain. This study used a Completely Randomized Design with 5 treatments and 3 individual replications. The treatments were combination between dose Oodev (PMSG and Anti-Dopamine) and dose Estradiol-17ß in feed (both doses in ml/kg feed):A(0+0), B(0.5+0), C(0+0.6), D(0.5+0,6), E(1+1). The results of this study showed that the administration of Oodev and Estradiol-17ß significant affect on gonadal maturity index about 82.21% compared to control and acclerating maturation of female mutiara strain with doses the best is the treatment E (oodev 1 ml/kg feed+estradiol-17ß 1 ml/kg feed).Key words: Clarias gariepinus, dose, estradiol-17ß, maturation, oodev

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PENGARUH FREKUENSI PENYIRAMAN AIR MENGGUNAKAN SPRINKLER TERHADAP RESPON FISIOLOGIS DAN PERTUMBUHAN SAPI PERANAKAN SIMMENTAL

Buletin Peternakan ◽

10.21059/buletinpeternak.v39i2.6715 ◽

2015 ◽

Vol 39 (2) ◽

pp. 109

Author(s):

Kusuma Adhianto ◽

Siswanto (Siswanto) ◽

Citra Nindya Kesuma

Keyword(s):

Experimental Design ◽

Key Words ◽

Physiological Response ◽

Animal Husbandry ◽

Animal Nutrition ◽

Randomized Design ◽

Feed Analysis ◽

Completely Randomized Design

The objective of the research was to measure the effects of watering frequency using sprinkler on the physiological response and production of simmental grade cattle. Experiment was conducted during 45 days starting on December 2014 to January 2015 in Gunung Madu Plantation Cooperative farms, Gunung Batin district, Central Lampung regency. Feed analysis was carried out in the Laboratory of Animal Nutrition and Feed, Department of Animal Husbandry, Faculty of Agriculture, University of Lampung. The experimental design used in this study was performed by using a Completely Randomized Design with treatment of control (P0), frequency of watering 1 time (P1) and frequency of watering 2 times (P2). Based on the results of research, we concluded that watering frequency treatment significantly (P<0.01) effected on the physiological response of Simmental grade cattle. However, treatment have not significantly effecting on consumption and growth of Simmental grade cattle.(Key words: Physiological response, Production, Sprinkler)

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