Modulation of Cell Surface Hydrophobicity and Attachment of Bacteria to Abiotic Surfaces and Shrimp by Malaysian Herb Extracts

2012 ◽  
Vol 75 (8) ◽  
pp. 1507-1511 ◽  
Author(s):  
YEW WOH HUI ◽  
GARY A. DYKES
Keyword(s):  
Stainless Steel ◽  
Foodborne Pathogens ◽  
Salmonella Enteritidis ◽  
Cell Surface Hydrophobicity ◽  
Surface Hydrophobicity ◽  
Bacterial Attachment ◽  
Water Extracts ◽  
Bacterial Hydrophobicity ◽  
Herb Extracts ◽  
Herb Extract

The use of simple crude water extracts of common herbs to reduce bacterial attachment may be a cost-effective way to control bacterial foodborne pathogens, particularly in developing countries. The ability of water extracts of three common Malaysian herbs (Andrographis paniculata, Eurycoma longifolia, and Garcinia atroviridis) to modulate hydrophobicity and attachment to surfaces of five food-related bacterial strains (Bacillus cereus ATCC 14576, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 10145, Salmonella Enteritidis ATCC 13076, Staphylococcus aureus ATCC 25923) were determined. The bacterial attachment to hydrocarbon assay was used to determine bacterial hydrophobicity. Staining and direct microscopic counts were used to determine attachment of bacteria to glass and stainless steel. Plating on selective media was used to determine attachment of bacteria to shrimp. All extracts were capable of either significantly (P < 0.05) increasing or decreasing bacterial surface hydrophobicity, depending on the herb extract and bacteria combination. Bacterial attachment to all surfaces was either significantly (P < 0.05) increased or decreased, depending on the herb extract and bacteria combination. Overall, hydrophobicity did not show a significant correlation (P > 0.05) to bacterial attachment. For specific combinations of bacteria, surface material, and plant extract, significant correlations (R > 0.80) between hydrophobicity and attachment were observed. The highest of these was observed for S. aureus attachment to stainless steel and glass after treatment with the E. longifolia extract (R = 0.99, P < 0.01). The crude water herb extracts in this study were shown to have the potential to modulate specific bacterial and surface interactions and may, with further work, be useful for the simple and practical control of foodborne pathogens.

Fibronectin enhances Campylobacter fetus interaction with extracellular matrix components and INT 407 cells

2008 ◽  
Vol 54 (1) ◽  
pp. 37-47 ◽  
Author(s):  
L. L. Graham ◽  
T. Friel ◽  
R. L. Woodman
Keyword(s):  
Extracellular Matrix ◽  
Solid Phase ◽  
Cell Surface Hydrophobicity ◽  
Surface Hydrophobicity ◽  
Bacterial Attachment ◽  
Binding Assays ◽  
Rgd Sequence ◽  
Campylobacter Fetus ◽  
Extracellular Matrix Components

Campylobacter fetus is a recognized pathogen of cattle and sheep that can also infect humans. No adhesins specific for C. fetus have to date been identified; however, bacterial attachment is essential to establish an infecting population. Scanning electron microscopy revealed C. fetus attachment to the serosal surface of human colonic biopsy explants, a location consistent with the presence of the extracellular matrix (ECM). To determine whether the ECM mediated C. fetus adherence, 7 C. fetus strains were assessed in a solid-phase binding assay for their ability to bind to immobilized ECM components. Of the ECM components assayed, adherence to fibronectin was noted for all strains. Attachment to ECM components was neither correlated with S-layer expression nor with cell-surface hydrophobicity. Ligand immunoblots, however, identified the S-layer protein as a major site of fibronectin binding, and modified ECM binding assays revealed that soluble fibronectin significantly enhanced the attachment of S-layer-expressing C. fetus strains to other ECM components. Soluble fibronectin also increased C. fetus adherence to INT 407 cells. This adherence was inhibited when INT 407 cells were incubated with synthetic peptides containing an RGD sequence, indicating that integrin receptors were involved in fibronectin-mediated attachment. Together, this data suggests that C. fetus can bind to immobilized fibronectin and use soluble fibronectin to enhance attachment to other ECM components and intestinal epithelial cells. In vivo, fibronectin would promote bacterial adherence, thereby, contributing to the initial interaction of C. fetus with mucosal and submucosal surfaces.

scholarly journals The Antimicrobial Effect of Radiant Catalytic Ionization on the Bacterial Attachment and Biofilm Formation by Selected Foodborne Pathogens under Refrigeration Conditions

2020 ◽  
Vol 10 (4) ◽  
pp. 1364
Author(s):  
Krzysztof Skowron ◽  
Karolina Jadwiga Skowron ◽  
Justyna Bauza-Kaszewska ◽  
Ewa Wałecka-Zacharska ◽  
Joanna Kwiecińska-Piróg ◽  
...  
Keyword(s):  
Foodborne Pathogens ◽  
Glass Surface ◽  
Biofilm Formation ◽  
Food Industry ◽  
Salmonella Enteritidis ◽  
Antimicrobial Effect ◽  
Bacterial Attachment ◽  
Efficiency Coefficient ◽  
Food Contact Surfaces ◽  
Reduction Efficiency

The decontamination of food contact surfaces is a major problem for the food industry. The radiant catalytic ionization (RCI) method, based on the ionization process, may be an alternative for conventional decontamination procedures. The advantage of this technique is the possibility of its application to household refrigerating appliances and industrial cold rooms. This study aimed to assess the effect of RCI on the reduction of Campylobacter jejuni, Listeria monocytogenes, and Salmonella Enteritidis from the biofilms formed on a glass surface under refrigeration conditions. Bacterial biofilms were exposed to RCI for 24 h and after 12 (variant I) and 72 h (variant II) of the glass surface contamination. In the last variant (III), the contaminated meat was placed on the glass surface in the refrigerator and subjected to RCI treatment for 72 h. The significantly highest values of absolute reduction efficiency coefficient E were found for the bacterial attachment stage of biofilm formation (variant I). The research proves the efficiency of the RCI method in the reduction of bacteria number from a glass surface.

Surface hydrophobicity of acidophilic heterotrophic bacterial cells in relation to their adhesion on minerals

1991 ◽  
Vol 37 (9) ◽  
pp. 692-696 ◽  
Author(s):  
B. K. Chakrabarti ◽  
P. C. Banerjee
Keyword(s):  
Heterotrophic Bacteria ◽  
Ph Value ◽  
Manganese Nodule ◽  
Cell Surface Hydrophobicity ◽  
Surface Hydrophobicity ◽  
Bacterial Attachment ◽  
Bacterial Cells ◽  
Iron Pyrite ◽  
Acidophilic Bacteria ◽  
Hydrophilic Nature

The cell-surface hydrophobicity of acidophilic heterotrophic bacteria originating from mines varied with the pH of the suspending medium and with the growth temperature. Adhesion of these bacterial cells on mineral particles depended upon the hydrophobic (or hydrophilic) nature of both the cells and the minerals. A strong correlation between these properties was usually observed at different pH values of the suspending medium. At a certain pH value, bacterial attachment depended upon the particle size of the minerals. Key words: hydrophobicity, acidophilic bacteria, Acidiphilium cryptum, Acidiphilium symbioticum, adhesion, manganese nodule, chalcopyrite, iron pyrite.

Physicochemical, Mechanical, and Molecular Properties of Nonlysogenic and P22-Lysogenic Salmonella Typhimurium Treated with Citrus Oil

2014 ◽  
Vol 77 (5) ◽  
pp. 758-764 ◽  
Author(s):  
JUHEE AHN ◽  
JOSE ALEJANDRO ALMARIO ◽  
SERAJUS SALAHEEN ◽  
DEBABRATA BISWAS
Keyword(s):  
Salmonella Typhimurium ◽  
Expression Patterns ◽  
Cell Surface Hydrophobicity ◽  
Surface Hydrophobicity ◽  
Bacterial Attachment ◽  
Swarming Motility ◽  
Heat Treated ◽  
Noticeable Reduction ◽  
Lysogenic Conversion ◽  
Sublethal Concentrations

The aim of this study was to evaluate the phenotypic and genotypic properties of nonlysogenic Salmonella Typhimurium (STP22−) and lysogenic Salmonella Typhimurium (STP22+) in the presence of sublethal concentrations (SLC2D) of citrus essential oils (CEOs), which were used to evaluate antimicrobial susceptibility, cell surface hydrophobicity, autoaggregation ability, bacterial motility, lysogenic conversion, gene expression patterns, and antibiofilm formation. The SLC2D values of non–heat-treated (N-CEO) and heat-treated (H-CEO) CEO in an autoclave at 121°C for 20 min were 2.0 to 2.1 mg/ml against STP22− and 1.7 to 1.9 mg/ml against STP22+. The rates of injured STP22− and STP22+ cells treated with SLC2D of N-CEO and H-CEO ranged from 67 to 83%. The hydrophobicity and autoaggregation were decreased to 2.5 and 19.5% for STP22− and 4.7 and 21.7% for STP22+, respectively, in the presence of N-CEO. A noticeable reduction in the swarming motility was observed in STP22− with N-CEO (14.5%) and H-CEO (13.3%). The numbers of CEO-induced P22 were 5.40 log PFU/ml for N-CEO and 5.65 log PFU/ml for H-CEO. The relative expression of hilA, hilC, hilD, invA, invC, invE, invF, sirA, and sirB was down-regulated in STP22− and STP22+ with N-CEO and H-CEO. The numbers of adherent STP22− and STP22+ were effectively reduced by more than 1 log in the presence of CEO. These results suggest that CEO has potential to be used to control bacterial attachment, colonization, and invasion.

Influence of Environmental Stress on the Kinetics and Strength of Attachment of Listeria monocytogenes Scott A to Buna-N Rubber and Stainless Steel

1998 ◽  
Vol 61 (10) ◽  
pp. 1286-1292 ◽  
Author(s):  
L. MICHELE SMOOT ◽  
MERLE D. PIERSON
Keyword(s):  
Stainless Steel ◽  
Listeria Monocytogenes ◽  
Growth Temperature ◽  
Cell Surface Hydrophobicity ◽  
Surface Hydrophobicity ◽  
Model Systems ◽  
Test Surface ◽  
Alkaline Conditions ◽  
Temperature And Growth ◽  
Growth Ph

Attachment and detachment of Listeria monocytogenes Scott A to Buna-N rubber and stainless Steel under varying conditions of temperature and pH were investigated using model systems. Numbers of attached cells increased with increasing attachment temperature (10 to 45°C) and time (up to 120 min) for both test surfaces. Compared to Buna-N rubber, the rate of attachment to stainless Steel was markedly more rapid for all temperature and pH conditions studied and could not be calculated. Rate of attachment to Buna-N rubber was found to be significantly lower when cells were attached at 10°C. Growth temperature did not significantly affect rates of adhesion to Buna-N rubber. Altering the medium pH during attachment between 4 and 9 demonstrated that rates of adhesion were slower under alkaline conditions. Growth pH was also found to significantly affect rates of attachment to Buna-N rubber. Detachment of cells adhered to Buna-N rubber was significantly affected by growth temperature but not growth pH. Significant differences in detachment were also found between Buna-N rubber and stainless Steel, inferring stronger attachment to Buna-N rubber. Cell surface hydrophobicity was found to be affected by both growth temperature and growth pH. However, changes in hydrophobicity could not be correlated to differences in rates of attachment. Addition of 0.01% trypsin to the attachment medium during cell exposure to either test surface resulted in a 99.9% reduction in the adhered cell population when compared to Controls. This would suggest that proteins play a role in the initial attachment process of L. monocytogenes.

scholarly journals Control of Multidrug-Resistant Pathogenic Staphylococci Associated with Vaginal Infection Using Biosurfactants Derived from Potential Probiotic Bacillus Strain

Fermentation ◽  
2022 ◽  
Vol 8 (1) ◽  
pp. 19
Author(s):  
Najla Haddaji ◽  
Karima Ncib ◽  
Wael Bahia ◽  
Mouna Ghorbel ◽  
Nadia Leban ◽  
...  
Keyword(s):  
Cell Surface Hydrophobicity ◽  
Surface Hydrophobicity ◽  
Multidrug Resistant ◽  
Bacterial Attachment ◽  
Bacterial Biofilm ◽  
Resistant Bacteria ◽  
Vaginal Infection ◽  
Adhesive Properties ◽  
Coating Agent ◽  
Bacterial Growth Inhibition

Biosurfactants exhibit antioxidant, antibacterial, antifungal, and antiviral activities. They can be used as therapeutic agents and in the fight against infectious diseases. Moreover, the anti-adhesive properties against several pathogens point to the possibility that they might serve as an anti-adhesive coating agent for medical inserts and prevent nosocomial infections, without using synthetic substances. In this study, the antimicrobial, antibiofilm, cell surface hydrophobicity, and antioxidative activities of biosurfactant extracted from Bacillus sp., against four pathogenic strains of Staphylococcus spp. associated with vaginal infection, were studied. Our results have shown that the tested biosurfactant possesses a promising antioxidant potential, and an antibacterial potency against multidrug clinical isolates of Staphylococcus, with an inhibitory diameter ranging between 27 and 37 mm, and a bacterial growth inhibition at an MIC of 1 mg/ mL, obtained. The BioSa3 was highly effective on the biofilm formation of different tested pathogenic strains. Following their treatment by BioSa3, a significant decrease in bacterial attachment (p < 0.05) was justified by the reduction in the optical (from 0.709 to 0.111) following their treatment by BioSa3. The antibiofilm effect can be attributed to its ability to alter the membrane physiology of the tested pathogens to cause a significant decrease (p < 0.05) of over 50% of the surface hydrophobicity. Based on the obtained result of the bioactivities in the current study, BioSa3 is a good candidate in new therapeutics to better control multidrug-resistant bacteria and overcome bacterial biofilm-associated infections by protecting surfaces from microbial contamination.

Relationship of Cell Surface Charge and Hydrophobicity to Strength of Attachment of Bacteria to Cantaloupe Rind†

2002 ◽  
Vol 65 (7) ◽  
pp. 1093-1099 ◽  
Author(s):  
DIKE O. UKUKU ◽  
WILLIAM F. FETT
Keyword(s):  
Cell Surface ◽  
Surface Charge ◽  
Cell Surface Hydrophobicity ◽  
Hydrophobic Interaction Chromatography ◽  
Surface Hydrophobicity ◽  
Bacterial Attachment ◽  
Wash Water ◽  
Bacterial Surface ◽  
E Coli ◽  
Salmonella Infections

The cantaloupe melon has been associated with outbreaks of Salmonella infections. It is suspected that bacterial surface charge and hydrophobicity may affect bacterial attachment and complicate bacterial detachment from cantaloupe surfaces. The surface charge and hydrophobicity of strains of Salmonella, Escherichia coli (O157:H7 and non-O157:H7), and Listeria monocytogenes were determined by electrostatic and hydrophobic interaction chromatography, respectively. Initial bacterial attachment to cantaloupe surfaces and the ability of bacteria to resist removal by washing with water were compared with surface charge and hydrophobicity. Whole cantaloupes were submerged in inocula containing individual strains or in cocktails containing Salmonella, E. coli, and L. monocytogenes, either as a mixture of strains containing all three genera or as a mixture of strains belonging to a single genus, for 10 min. Inoculated cantaloupes were dried for 1 h in a biosafety cabinet and then stored for up to 7 days at 4°C. Inoculated melons were washed with water, and bacteria still attached to the melon surface, as well as those in the wash water, were enumerated. Initial bacterial attachment was highest for individual strains of E. coli and lowest for L. monocytogenes, but Salmonella exhibited the strongest attachment on days 0, 3, and 7. When mixed-genus cocktails were used, the relative degrees of attachment of the three genera ware altered. The attachment of Salmonella strains was the strongest, but the attachment of E. coli was more extensive than that of L. monocytogenes on days 0, 3, and 7. There was a linear correlation between bacterial cell surface hydrophobicity (r2 = 0.767), negative charge (r2 = 0.738), and positive charge (r2 = 0.724) and the strength of bacterial attachment to cantaloupe surfaces.

Effects of the Growth Procedure on the Surface Hydrophobicity of Listeria monocytogenes Cells and Their Adhesion to Stainless Steel

1999 ◽  
Vol 62 (9) ◽  
pp. 994-998 ◽  
Author(s):  
ROMAIN BRIANDET ◽  
VALERIE LERICHE ◽  
BRIGITTE CARPENTIER ◽  
MARIE-NOELLE BELLON-FONTAINE
Keyword(s):  
Stainless Steel ◽  
Lactic Acid ◽  
Listeria Monocytogenes ◽  
Electron Acceptor ◽  
Surface Hydrophobicity ◽  
Bacterial Attachment ◽  
Hydrophobic Properties ◽  
Cured Meat ◽  
The Media ◽  
Donor Characteristics

The aim of this study was to examine the physicochemical surface properties and the ability to adhere to stainless steel of three strains of Listeria monocytogenes after different cultivation procedures. To this end, bacteria were cultivated at 37°C after storage at two frequently used temperatures (4°C or −80°C) and were then transferred into the liquid medium (trypticase soy broth supplemented with 6 g liter−1 of yeast extract, pH 7.3) between one and four times. In addition, the influence of supplementing the growth medium with lactic acid was explored, this organic acid being representative of both the dairy and cured meat industries. The hydrophobic/hydrophilic and electron-acceptor/electron-donor characteristics of the strains were evaluated by the microbial adhesion to solvents method. Using this technique, we recorded an increase in the hydrophobic properties of one strain stored at 4°C, with an increasing number of transfers in the media (P &lt; 0.05). Another plant-isolated strain appeared more hydrophobic and stuck better to stainless steel when cells were stored at 4°C rather than at −80°C. Preculturing L. monocytogenes in a lactic acid-supplemented medium increased the affinity of microbial cells to solvents and the bacterial attachment to stainless steel (P &lt; 0.05).

scholarly journals Silver implantation on AISI 304 stainless steel surface using low-energy doses and the antimicrobial effect against Salmonella Enteritidis and Listeria monocytogenes

2020 ◽  
Vol 5 (5) ◽  
pp. 244-251
Author(s):  
Caroline Isabel Kothe ◽  
◽  
Regina Zilio ◽  
Tatiana Pacheco Soares Zamboni ◽  
Cesar Aguzzoli ◽  
...  
Keyword(s):  
Stainless Steel ◽  
Foodborne Pathogens ◽  
Salmonella Enteritidis ◽  
Steel Surface ◽  
Silver Ions ◽  
Low Energy ◽  
304 Stainless Steel ◽  
Stainless Steel Surface ◽  
Aisi 304 Stainless Steel ◽  
Aisi 304

Silver has antimicrobial properties and when implemented on the stainless steel surface can inactivate microorganisms and consequently prevent biofilm formation and cross-contamination of food. Therefore, in this study we evaluated the antibacterial properties of silver ions implanted on AISI 304 stainless steel surfaces using low-energy doses against Salmonella Enteritidis and Listeria monocytogenes, two foodborne pathogens. AISI 304 stainless steel coupons were treated using energy of 2 and 4 keV for silver implantation and simulations were performed to estimate its dose distribution. Coupons containing silver ions were contaminated with S. Enteritidis and L. monocytogenes and incubated at 25 °C for 1 and 24 h. Results demonstrated that 4 keV treatment were able to reduce S. Enteritidis, but not L. monocytogenes. However, the 2 keV treatment showed significant reductions of both pathogens and the depth profiles of surfaces treated with 2 keV of energy showed 3.5×1016 silver atoms/cm² implanted in up to 5 nm from the stainless steel surface. Silver implanted on stainless steel using low-energy doses demonstrated antimicrobial properties against foodborne pathogens and this strategy can be used to reduce adhered cells and biofilm formation in food industries. Keywords: bacterial adhesion, biofilm, foodborne pathogens, ion implantation, silver ions

scholarly journals Evaluation of the Adhesive Potential of Bacteria Isolated from Meat-Related Sources

2021 ◽  
Vol 11 (22) ◽  
pp. 10652
Author(s):  
Zhenzhen Ning ◽  
Bei Xue ◽  
Huhu Wang
Keyword(s):  
Food Processing ◽  
Control Strategies ◽  
Cell Surface Hydrophobicity ◽  
Surface Hydrophobicity ◽  
Aeromonas Salmonicida ◽  
Pseudomonas Fragi ◽  
Hydrophobic Force ◽  
Aeromonas Veronii ◽  
High Level ◽  
Bacterial Hydrophobicity

Microbial adhesion constitutes the transition of microorganisms from a planktonic mode to a static one. It promotes the formation of biofilm which is responsible for spoilage, foodborne diseases, and corrosion in the food processing industry. In this study, the adhesive potential of fourteen meat-borne bacterial isolates belonging to seven different genera was investigated. All strains were found able to colonize polystyrene surfaces with different levels of firmness. Significant variations were determined in assays of bacterial hydrophobicity and motility. Among the 14 strains, Pseudomonas fragi, Aeromonas salmonicida II, Serratia liquefaciens, Citrobacter braakii, Pseudomonas putida, and Aeromonas veronii had a strong hydrophobic force, while the isolates of Lactobacillus genus showed the most hydrophilic property. In terms of motility, Citrobacter braakii and Escherichia coli exhibited exceptional swarming and swimming abilities, whilst conservatively weak performances were observed in the Lactobacillus strains. Furthermore, the majority of the isolates were predominantly electron donors and weak electron acceptors. Overall, a high level of correlation was observed between biofilm-forming ability with cell surface hydrophobicity and Lewis acid–base properties, whereas the contribution of motility in bacterial adhesion could not be confirmed. Research on the adhesive performance of foodborne bacteria is potentially conducive to developing novel control strategies, such as food processing equipment with specific surfaces, not facilitating attachment.

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