Detection of Fish Bones in Cod Fillets by UV Illumination

2015 ◽  
Vol 78 (7) ◽  
pp. 1414-1419 ◽  
Author(s):  
SHENG WANG ◽  
RUI NIAN ◽  
LIMIN CAO ◽  
JIANXIN SUI ◽  
HONG LIN

The presence of fish bones is now regarded as an important hazard in fishery products, and there is increasing demand for new analytical techniques to control it more effectively. Here, the fluorescent properties of cod bones under UV illumination were investigated, and the maximal wavelengths for excitation and emission were determined to be 320 nm and 515 nm, respectively, demonstrating significantly different fluorescence characteristics and much higher fluorescence intensity compared to those of fillet muscles. Based on the results, UV fluorescence-assisted candling for the detection of bones in fishery products was developed for the first time. Using cod fillets as samples, the detection ratio of this technique was calculated as 90.86%, significantly higher than that of traditional candling under daylight (76.78%). Moreover, the working efficiency of the new technique was about 26% higher than that of the traditional method. A UV fluorescence imaging framework was also developed, and a method for automatic identification of the fish bones in the cod fillets based on the linear discriminant analysis proposed by Fisher was preliminarily realized, but the detection ratio was demonstrated to be relatively poor compared to those of candling techniques. These results allow us to suggest UV-based methods as new and promising approaches for routine monitoring of bones in fishery products.

Author(s):  
Zbigniew Omiotek

The purpose of the study was to construct an efficient classifier that, along with a given reduced set of discriminant features, could be used as a part of the computer system in automatic identification and classification of ultrasound images of the thyroid gland, which is aimed to detect cases affected by Hashimoto’s thyroiditis. A total of 10 supervised learning techniques and a majority vote for the combined classifier were used. Two models were proposed as a result of the classifier’s construction. The first one is based on the K-nearest neighbours method (for K = 7). It uses three discriminant features and affords sensitivity equal to 88.1%, specificity of 66.7% and classification error at a level of 21.8%. The second model is a combined classifier, which was constructed using three-component classifiers. They are based on the K-nearest neighbours method (for K = 7), linear discriminant analysis and a boosting algorithm. The combined classifier is based on 48 discriminant features. It allows to achieve the classification sensitivity equal to 88.1%, specificity of 69.4% and classification error at a level of 20.5%. The combined classifier allows to improve the classification quality compared to the single model. The models, built as a part of the automatic computer system, may support the physician, especially in first-contact hospitals, in diagnosis of cases that are difficult to recognise based on ultrasound images. The high sensitivity of constructed classification models indicates high detection accuracy of the sick cases, and this is beneficial to the patients from a medical point of view.


2020 ◽  
Vol 10 (23) ◽  
pp. 8347
Author(s):  
Florina-Dorina Covaciu ◽  
Camelia Berghian-Grosan ◽  
Ioana Feher ◽  
Dana Alina Magdas

This study proposes a comparison between two analytical techniques for edible oil classification, namely gas-chromatography equipped with a flame ionization detector (GC-FID), which is an acknowledged technique for fatty acid analysis, and Raman spectroscopy, as a real time noninvasive technique. Due to the complexity of the investigated matrix, we used both methods in connection with chemometrics processing for a quick and valuable evaluation of oils. In addition to this, the possible adulteration of investigated oil varieties (sesame, hemp, walnut, linseed, sea buckthorn) with sunflower oil was also tested. In order to extract the meaningful information from the experimental data set, a supervised chemometric technique, namely linear discriminant analysis (LDA), was applied. Moreover, for possible adulteration detection, an artificial neural network (ANN) was also employed. Based on the results provided by ANN, it was possible to detect the mixture between sea buckthorn and sunflower oil.


1997 ◽  
Vol 45 (1) ◽  
pp. 1 ◽  
Author(s):  
Peter J. Dunlop ◽  
Caroline M. Bignell ◽  
D. Brynn Hibbert

Using morphological observations, botanists have classified Eucalyptus species into characteristic series. A new vacuum distillation technique has been employed to obtain the characteristic leaf oils, which are very close to their in vivo compositions, from 35 species belonging to series Tetrapterae, series Torquatae and series Rufispermae. Accurate gas chromatograms have been obtained for each species and three analytical techniques (principal component analysis (PCA), hierarchical cluster analysis (CA) and linear discriminant analysis (LDA)) have been used to process these chromatograms to see if agreement with these classifications could be achieved without using any auxiliary morphometric data. For the species chosen for the present study, linear discriminant analysis was the most successful in assigning species to their present botanic classifications. These analytical methods were also used with some success in searching for groupings within a series and within a species.


2018 ◽  
Vol 26 (4) ◽  
pp. 1455 ◽  
Author(s):  
Bárbara Helohá Falcão Teixeira ◽  
Maryualê Malvessi Mittmann

Abstract: This work presents the results of the analysis of multiple acoustic parameters for the construction of a model for the automatic segmentation of speech in tone units. Based on literature review, we defined sets of acoustic parameters related to the signalization of terminal and non-terminal boundaries. For each parameter, we extracted a series of measurements: 6 for speech rate and rhythm; 34 for duration; 65 for fundamental frequency; 4 for intensity and 2 measurements related to pause. These parameters were extracted from spontaneous speech fragments that were previously segmented into tone units, manually performed by 14 human annotators. We used two methods of statistical classification, Random Forest (RF) and Linear Discriminant Analysis (LDA), to generate models for the identification of prosodic boundaries. After several phases of training and testing, both methods were relatively successful in identifying terminal and non-terminal boundaries. The LDA method presented a higher accuracy in the prediction of terminal and non-terminal boundaries than the RF method, therefore the model obtained with LDA was further refined. As a result, the terminal boundary model is based on 20 acoustic measurements and shows a convergence of 80% in relation to boundaries identified by annotators in the speech sample. For non-terminal boundaries, we arrived at three models that, combined, presented a convergence of 98% in relation to the boundaries identified by annotators in the sample.Keywords: speech segmentation; prosodic boundaries; spontaneous speech.Resumo: Este trabalho apresenta os resultados da análise de múltiplos parâmetros acústicos para a construção de um modelo para a segmentação automática da fala em unidades tonais. A partir da investigação da literatura, definimos conjuntos de parâmetros acústicos relacionados à identificação de fronteiras terminais e não terminais. Para cada parâmetro, uma série de medidas foram extraídas: 6 medidas de taxa de elocução e ritmo; 34 de duração; 65 de frequência fundamental; 4 de intensidade e 2 medidas relativas às pausas. Tais parâmetros foram extraídos de fragmentos de fala espontânea previamente segmentada em unidades tonais de forma manual por 14 anotadores humanos. Utilizamos dois métodos de classificação estatística, Random Forest (RF) e Linear Discriminant Analysis (LDA), para gerar modelos de identificação de fronteiras prosódicas. Após diversas fases de treinamentos e testes, ambos os métodos apresentaram sucesso relativo na identificação de fronteiras terminais e não-terminais. O método LDA apresentou maior índice de acerto na previsão de fronteiras terminais e não-terminais do que o RF, portanto, o modelo obtido com este método foi refinado. Como resultado, O modelo para as fronteiras terminais baseia-se em 20 medidas acústicas e apresenta uma convergência de 80% em relação às fronteiras identificadas pelos anotadores na amostra de fala. Para as fronteiras não terminais, chegamos a três modelos que, combinados, apresentaram uma convergência de 98% em relação às fronteiras identificadas pelos anotadores na amostra.Palavras-chave: segmentação da fala; fronteiras prosódicas; fala espontânea.


2016 ◽  
Vol 55 (06) ◽  
pp. 533-544 ◽  
Author(s):  
Pedro Benito ◽  
María Hernando ◽  
Fernando García-García

SummaryBackground: Physical activity (PA) is essential to prevent and to treat a variety of chronic diseases. The automated detection and quantification of PA over time empowers lifestyle interventions, facilitating reliable exercise tracking and data-driven counseling.Methods: We propose and compare various combinations of machine learning (ML) schemes for the automatic classification of PA from multi-modal data, simultaneously captured by a biaxial accelerometer and a heart rate (HR) monitor. Intensity levels (low / moderate / vigorous) were recognized, as well as for vigorous exercise, its modality (sustained aerobic / resistance / mixed). In to -tal, 178.63 h of data about PA intensity (65.55 % low / 18.96 % moderate / 15.49 % vigorous) and 17.00 h about modality were collected in two experiments: one in free- living conditions, another in a fitness center under controlled protocols. The structure used for automatic classification comprised: a) definition of 42 time-domain signal features, b) dimensionality reduction, c) data clustering, and d) temporal filtering to exploit time redundancy by means of a Hidden Markov Model (HMM). Four dimensionality reduction techniques and four clustering algorithms were studied. In order to cope with class imbalance in the dataset, a custom performance metric was defined to aggregate recognition accuracy, precision and recall.Results: The best scheme, which comprised a projection through Linear Discriminant Ana -lysis (LDA) and k-means clustering, was evaluated in leave-one-subject-out cross-validation; notably outperforming the standard industry procedures for PA intensity classification: score 84.65 %, versus up to 63.60 %. Errors tended to be brief and to appear around transients.Conclusions: The application of ML techniques for pattern identification and temporal filtering allowed to merge accelerometry and HR data in a solid manner, and achieved markedly better recognition performances than the standard methods for PA intensity estimation.


Metals ◽  
2019 ◽  
Vol 9 (5) ◽  
pp. 546 ◽  
Author(s):  
Dayakar L. Naik ◽  
Hizb Ullah Sajid ◽  
Ravi Kiran

Automatic identification of metallurgical phases based on thresholding methods in microstructural images may not be possible when the pixel intensities associated with the metallurgical phases overlap and, hence, are indistinguishable. To circumvent this problem, additional visual information about the metallurgical phases, referred to as textural features, are considered in this study. Mathematically, textural features are the second order statistics of an image domain and can be distinct for each metallurgical phase. Textural features are evaluated from the gray level co-occurrence matrix (GLCM) of each metallurgical phase (ferrite, pearlite, and martensite) present in heat-treated ASTM A36 steels in this study. The dataset of textural features and pixel intensities generated for the metallurgical phases is used to train supervised machine learning classifiers, which are subsequently employed to predict the metallurgical phases in the microstructure. Naïve Bayes (NB), k-nearest neighbor (K-NN), linear discriminant analysis (LDA), and decision tree (DT) classifiers are the four classifiers employed in this study. The performances of all four classifiers were assessed prior to their deployment, and the classification accuracy was found to be >97%. The proposed technique has two unique advantages: (1) unlike pixel intensity-based methods, the proposed method does not misclassify the grain boundaries as a metallurgical phase, and (2) the proposed method does not require the end-user to input the number of phases present in the microstructure.


2020 ◽  
Author(s):  
Most. Sheuli Akter ◽  
Md. Rabiul Islam ◽  
Yasushi Iimura ◽  
Hidenori Sugano ◽  
Kosuke Fukumori ◽  
...  

Presurgical investigations for categorizing focal patterns are crucial, leading to localization and surgical removal of the epileptic focus. This paper presents a machine learning approach using information theoretic features extracted from high-frequency subbands to detect the epileptic focus from interictal intracranial electroencephalogram (iEEG). It is known that high-frequency subbands (>80 Hz) include important biomarkers such as high-frequency oscillations (HFOs) for identifying epileptic focus commonly referred to as the seizure on-set zone (SOZ). In this analysis, the multi-channel interictal iEEG signals were splitted into segments and each segment was decomposed into multiple high-frequency subbands. The different types of entropy were calculated for each of the subbands and the sparse linear discriminant analysis (sLDA) was applied to select the prominent entropy features. Due to the imbalance of SOZ and non-SOZ channels in iEEG data, the use of machine learning techniques is always tricky. To deal with the imbalanced learning problem, an adaptive synthetic oversampling approach (ADASYN) with radial basis function kernel-based SVM was used to detect the focal segments. Finally, the epileptic focus was identified based on detection of focal segments on SOZ and non-SOZ channels. Eight patients were examined to observe the efficiency of the automatic detector. The experimental results and statistical tests indicate that the proposed automatic detector can identify the epileptic focus accurately and efficiently.


2013 ◽  
Vol 76 (7) ◽  
pp. 1288-1292 ◽  
Author(s):  
XIANLIN YANG ◽  
RUI NIAN ◽  
HONG LIN ◽  
CUI DUAN ◽  
JIANXIN SUI ◽  
...  

Anisakid larvae are regarded as an important hazard in marine products, and demand is growing for on-line nondestructive analytical techniques for effective monitoring of these parasites. A UV fluorescent imaging was developed for detection of the third-stage anisakid larvae in marine fishes, and different processing methods were investigated and optimized based on principal component and gray value analyses. Using cod fillets as samples, the efficiency of the developed technique was evaluated, and the overall detection ratio was greater than 80%. These results indicate a promising application of UV fluorescent imaging as an effective and nondestructive technique for identification of anisakid larvae in fishery products.


2019 ◽  
Vol 89 (3) ◽  
pp. 438-445 ◽  
Author(s):  
Connie Lai ◽  
Peter J. Bush ◽  
Stephen Warunek ◽  
David A. Covell ◽  
Thikriat Al-Jewair

ABSTRACT Objectives: To assess the effectiveness and efficiency of ultraviolet (UV) illumination compared to conventional white light in the detection of fluorescent-tagged adhesive remnants during orthodontic debonding. Materials and Methods: Orthodontic brackets were bonded to extracted human premolars using one of two bonding resins having fluorescent properties (Pad Lock, Reliance Orthodontics, Itasca, Ill; Opal Bond MV, Opal Orthodontics, South Jordan, Utah; n = 40 each). The brackets were then debonded and, in each adhesive group, half the teeth had the remaining adhesive resin removed under illumination using the operatory light and the other half using a UV (395 nm) light emitting diode (LED) flashlight (n = 20/group). Time for teeth cleanup was recorded. Follow-up images were obtained under a dissecting microscope using UV illumination, and the surface area of adhesive remnants was calculated. Effectiveness of adhesive removal was also assessed using scanning electron microscopy imaging. Analysis of variance and Kruskal-Wallis tests were used to analyze time and adhesive remnants, respectively. Results: Assessment using the dissecting microscope found groups using UV light during adhesive removal had statistically significantly lower amounts of adhesive remnants than groups using white light (P ≤ .01). Time for adhesive removal was significantly lower with Opal Bond MV adhesive using UV light when compared with the white light (P ≤ .01). Assessment by scanning electron microscopy showed that thin remnants of adhesive (<2 μm) remained undetected by UV illumination. Conclusions: UV light is more effective and tends to be more efficient than white light in the detection of fluorescent adhesive during orthodontic debonding. Although there are limitations, the use of UV LED lighting is a practical tool that aids in adhesive detection.


2019 ◽  
Author(s):  
Yingxian Chen ◽  
Livia Elena Crică ◽  
Vinicio Rosano ◽  
Adrián Esteban Arranz ◽  
David Spiller ◽  
...  

AbstractGraphene oxide (GO) holds great potential for biomedical applications, however fundamental understanding of the way it interacts with biological systems is still lacking even though it is a prerequisite for successful clinical translation. In this study, we exploited intrinsic fluorescent properties of GO to establish the relationship between lateral dimensions of the material, its cellular uptake mechanism and intracellular fate. Label-free GO with distinct lateral dimensions, small (s-GO) and ultra-small (us-GO), was synthesized and thoroughly characterised both in water and in biologically relevant cell culture medium. Interactions of the material with a range of non-phagocytic mammalian cell lines (BEAS-2B, NIH/3T3, HaCaT, 293T) were studied using a combination of complementary analytical techniques (confocal microscopy, flow cytometry and TEM). The uptake mechanism was interrogated using a range of pharmaceutical inhibitors for main endocytic pathways (ethyl-isopropyl amiloride, monodansylcadaverine, chlorpromazine, genistein, cytochalasin D, latrunculin A, dynasore and sodium azide), and validated using negatively charged polystyrene beads with different diameters (0.1 and 1 μm). Regardless of lateral dimension, both types of GO were found to interact with the plasma membrane and to be efficiently taken up by a panel of cell lines in a time- and dose-dependent manner. s-GO was internalised mainly via macropinocytosis while us-GO used mainly clathrin- and caveolae-mediated endocytosis. Lastly, we show that both s-GO and us-GO terminate in lysosomal compartments for up to 48 h. Our results aim to offer significant insight into the mechanism of interaction of GO with non-phagocytic cell lines that can be exploited for the design of biomedically applicable 2D transport systems.


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