Impact of MS concentration and light qualities on alpine red ginger elongation and rooting in vitro

Endophytic fungi isolated from red ginger (Zingiber officinale) can inhibit growth of Fusarium oxysporum, but the ability of the fungi to control Candida albicans as human pathogen has not been reported. The aims of this research were to study the mechanism of ten endophytic fungi isolates derived from red ginger to control C. albicans in vitro using dual culture methode and fungal extract, and to determine fungal bioactive chemical groups produced by the fungi. Three out of ten isolates tested, Talaromyces assiutensis JMa 7, T. assiutensis JMbt 3, and Curvularia affinis JMbt 9 inhibited growth of C. albicans with inhibition zones were 4.0 mm, 4.9 mm, and 11.3 mm, respectively. The cultures of the three potential endophytic fungi were extracted by maceration method using 3 solvents i.e ethyl acetate, n-hexane and ethanol. The three isolates were grown in PDB separately for 21 days incubation. At harvest, the culture filtrate was extracted by ethyl acetate and n-hexane, while fungal mycelia were extracted by all the three solvents. Ethyl acetate extracts obtained from culture filtrate of all the three fungal isolates consistently inhibited C. albicans with inhibition zones were 2.0-3.8 mm. For n-hexane extract, however, only Talaromyces assiutensis JMbt 3 that had positive effect with inhibition zone was 2.0 mm. All extracts from mycelia did not have any effects on C. albicans. The ethyl acetate extract of T. assiutensis JMbt 3 was analysed to determine its chemical groups using visible color on thin layer chromatography (TLC). The results showed that the bioactive compounds was terpenoids, and antioxidant.

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Phytobiotic Properties of Garlic, Red Ginger, Turmeric and Kencur in Growing Ducks

ANIMAL PRODUCTION ◽

10.20884/1.anprod.2015.17.1.484 ◽

2015 ◽

Vol 17 (1) ◽

pp. 49 ◽

Cited By ~ 2

Author(s):

Ismoyowati Ismoyowati ◽

Diana Indrasanti ◽

Mochamad Mufti ◽

Abdoreza Soleimani Farjam

Keyword(s):

Escherichia Coli ◽

Allium Sativum ◽

Zingiber Officinale ◽

Feed Conversion ◽

Feeding Trial ◽

E Coli ◽

Salmonella Pullorum ◽

Red Ginger

Abstract. Phytobiotic properties of garlic (Allium sativum), turmeric (Curcuma domestica), red ginger (Zingiber officinale) and kencur (Kaempferia galangal) were studied using standard in vitro antibacterial test and in vivo feeding trial with ducklings. In the in vitro experiment, potency of aqueous extract of these phytobiotic agents were tested against Salmonella pullorum and Escherichia coli. Feeding trial was carried out for 6 week starting at day 28 using ducklings fed diets supplemented with 1% of each of four phytobiotic agents. The highest antibacterial activity against S. pullorum and E. coli was observed with garlic and no additive effect when mixture of phytobiotics was used. Weight gain, fed intake and feed conversion ratio of ducklings were not affected by inclusion of garlic, red ginger and kencur. However, 1% turmeric supplementation significantly reduced growth performance to ducklings. Key words: phytobiotic, antibiotic, duck, medicinal plants Abstrak. Penelitian karakteristik fitobiotik dari bawang putih (Allium sativum), kunyit (Curcuma domestica), jahe merah (Zingiber officinale) dan kencur (Kaempferia galangal) telah dilakukan secara in vitro melalui uji aktivitas antibakteri dan secara in vivo dengan perlakuan suplemetasi fitobiotik didalam pakan anak itik. Pada percobaan in vitro, potensi aktivitas antibakteri dari ektrak fitobiotik diuji menggunakan Salmonella pullorum and Escherichia coli. Percobaan suplementasi fitobiotik diberikan masing-masing sebesar 1% didalam pakan anak itik. Pemberian pakan perlakuan dilakukan selama 6 minggu, dimulai pada saat anak itik berumur 28 hari. Hasil penelitian menunjukkan aktivitas antibakteri terhadap S. pullorum dan E. coli paling tinggi adalah ektrak bawang putih dan tidak ada pengaruh yang lebih baik apabila dicampur dengan ektrak fitobiotik lainnya. Pertambahan bobot badan, konsumsi pakan dan konversi pakan anak itik tidak dipengaruhi oleh penambahan bawang putih, kunyit, jahe merah dan kencur. Akan tetapi, suplementasi kunyit nyata menurunkan performan pertumbuhan anak itik. Kata kunci: fitobiotik, antibiotik, itik, tanaman obat-obatan.

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Antioxidant and inhibitory effect of red ginger (Zingiber officinale var. Rubra) and white ginger (Zingiber officinale Roscoe) on Fe2+ induced lipid peroxidation in rat brain in vitro

Experimental and Toxicologic Pathology ◽

10.1016/j.etp.2010.06.002 ◽

2012 ◽

Vol 64 (1-2) ◽

pp. 31-36 ◽

Cited By ~ 60

Author(s):

Ganiyu Oboh ◽

Ayodele J. Akinyemi ◽

Adedayo O. Ademiluyi

Keyword(s):

Lipid Peroxidation ◽

Rat Brain ◽

Zingiber Officinale ◽

Inhibitory Effect ◽

Zingiber Officinale Roscoe ◽

Red Ginger

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Study of Local Herb Potency as Rumen Modifier: Red Ginger (Zingiber Officinale Var. Rubrum) Addition Effect on In Vitro Ruminal Nutrient Digestibility

ANIMAL PRODUCTION ◽

10.20884/1.jap.2019.21.1.713 ◽

2020 ◽

Vol 21 (1) ◽

pp. 30

Author(s):

Asih Kurniawati ◽

Lies Mira Yusiati ◽

Widodo Widodo ◽

Wayan Tunas Artama

Keyword(s):

Essential Oil ◽

Zingiber Officinale ◽

Fermentation Medium ◽

Gas Production ◽

Nutrient Digestibility ◽

Treatment Level ◽

Production Volume ◽

Nutrient Analysis ◽

Red Ginger

Red ginger is herb that commonly used as part of traditional medicine due to its essential oil content. Some essential oil have proven as rumen fermentation modifier. Addition of red ginger in ruminant diet was studied using in vitro gas production technique to evaluate its effect on nutrient digestibility. Red ginger meal was added to meet essential oil level in fermentation medium of 0 mg/l as control, 25, 50, 75 and 100 mg/l. The diet consisted of Penisetum hybride, rice bran, wheat pollard in ratio 60:20:20 DM basis. Feed fermentation was incubated for 24 h at 39°C. At the end of incubation data of gas production volume was taken, and residual feed were collected for further nutrient analysis to calculate the digestibility of dry matter (DM), organic matter (OM), crude protein (CP) and crude fiber (CF). Variance analysis was used to analysis collected data, followed by DMRT analysis. Addition of red ginger increase total volume of gas production at level 50 mg/L and above. DM and OM digestibility did not affected by red ginger addition whereas CP digestibility were significantly decreased start at level 50 mg/l. In contrast, CF digestibility of treatment level of 50 mg/l significantly higher than control. In conclusion, addition of red ginger correspond to level essential oil of 50 to 100 mg/l improve ruminal nutrient fermentation.

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Antibacterial Activity Test of Red Ginger Extract (Zingiber officinale var. Rubrum) Against Streptococcus pyogenes In vitro

Biomolecular and Health Science Journal ◽

10.20473/bhsj.v3i1.19130 ◽

2020 ◽

Vol 3 (1) ◽

pp. 24

Author(s):

Samira Assegaf ◽

Arthur Pohan Kawilarang ◽

Retno Handajani

Keyword(s):

Antibacterial Activity ◽

Streptococcus Pyogenes ◽

Zingiber Officinale ◽

Positive Control ◽

Bacterial Suspension ◽

Ginger Extract ◽

Mueller Hinton ◽

Red Ginger ◽

Mueller Hinton Broth

Introduction: Red ginger (Zingiber officinale var. rubrum) is known as an antibacterial agent. Streptococcus pyogenes is the most common bacterial cause of pharyngitis in children and adults. Over the years, antibiotic resistance case is increasing. Therefore, further research of other substances to find an bactericidal or bacteriostatic agent is needed. The aim of this study is to determine the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of Z. officinale var. rubrum against S. pyogenes. Methods: Serial dilution test of red ginger extract in Mueller hinton broth (8 concentrations of red ginger extract : 80%, 40%, 20%, 10%, 5%, 2,5%, 1,25%, and 0,625%) and positive control (1 ml bacterial suspension in Mueller hinton broth) was used to determine MIC. MBC was determined by culturing solutions from the previous dilution test into blood agar plate.lResults: MIC of red ginger extract against S. pyogenes could not be determined because the color of red ginger extract affected the turbidity of the dilution test result. MBC of red ginger extract against S. pyogenes was at concentration 20% of red ginger extract.Conclusion: Z. officinale var. rubrum showed antibacterial activity against S. pyogenes.

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Rhizome Buds Disinfection for Preparation of Red Ginger (Zingiber officinale Roxb. var. rubrum Rosc.) In Vitro Culture

Media Pharmaceutica Indonesiana (MPI) ◽

10.24123/mpi.v3i1.2836 ◽

2020 ◽

Vol 3 (1) ◽

pp. 19-26

Author(s):

Popy Hartatie Hardjo ◽

Alfian Hendra Krisnawan

Keyword(s):

In Vitro Culture ◽

Axenic Culture ◽

Zingiber Officinale ◽

Culture Initiation ◽

Surface Sterilization ◽

Sterilization Method ◽

Ginger Rhizome ◽

Red Ginger ◽

Rhizome Buds

The success of culture initiation depends on explant surface sterilization techniques. Suitable concentration, combinations, and duration of exposure of sterilizing agents are important to raise in vitro culture successfully. The aim of this work is to obtain the suitable sterilization method for explant buds of red ginger rhizome to get the axenic culture. Four sterilizing agents, fungicide, bactericide, Cefotaxime antibiotic, and NaOCl were tested for sterilization by various concentration and duration of exposure. The results showed that sterilizing agents 200 mg/L Cefotaxime and 100 mg/L Benomyl combined with NaOCl decreased the contamination of explants, and achieved 20% axenic culture.

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Propagação in vitro de cultivares de alpinia em diferentes fontes de luz

Ornamental Horticulture ◽

10.14295/oh.v25i1.1255 ◽

2019 ◽

Vol 25 (1) ◽

pp. 49-54

Author(s):

Marcos Vinícius Marques Pinheiro ◽

Denise Schmidt ◽

Maria Inês Diel ◽

Jullie Dos Santos ◽

Leonardo Antonio Thiesen ◽

...

Keyword(s):

In Vitro Propagation ◽

Plant Production ◽

Experimental Unit ◽

Light Sources ◽

White Led ◽

Light Emitting ◽

Randomized Design ◽

Red Ginger ◽

Alpinia Purpurata

The light source in tissue culture is often one of the punctual factors for the adequacy of plant production. For this, light emitting diodes (LEDs) have been used in order to increase the growth and development of the plants propagated in vitro. The objective was to evaluate the in vitro propagation of cultivars of Alpinia purpurata ‘Red Ginger’ and ‘Pink Ginger’ in two different light sources (fluorescent or LEDs tubes). The experiment was conducted in a completely randomized design, factorial scheme (2x2), totaling four treatments with ten replicates, and the experimental unit composed of one flask containing five explants. After 55 days, white LED and the ‘Red Ginger’ provided the highest number of shoots. For the number of leaves, the cultivar ‘Red Ginger’ and white LED lamps provided superior results. For shoot length (cm), the cultivar ‘Red Ginger’ kept under fluorescent lamps was superior, and ‘Pink Ginger’ presented superiority when cultivated under LED lights. Thus, even with differentiated responses among genotypes, the use of white LED lamps in the in vitro propagation of Alpinia purpurata ‘Red Ginger’ and ‘Pink Ginger’ cultivars is suggested.

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ISOLASI SENYAWA AKTIF DAN UJI AKTIVITAS EKSTRAK JAHE MERAH (ZINGIBER OFFICINALE) SEBAGAI IMUNOMODULATOR

Jurnal Riset Kefarmasian Indonesia ◽

10.33759/jrki.v3i2.131 ◽

2021 ◽

Vol 3 (2) ◽

pp. 77-91

Author(s):

Masniah Masniah ◽

Jafril Rezi ◽

Ahmad Purnawarman Faisal

Keyword(s):

Lymphocyte Proliferation ◽

Ethanol Extract ◽

Zingiber Officinale ◽

Immunomodulatory Activity ◽

Ginger Extract ◽

Immunostimulatory Activity ◽

Macrophage Cells ◽

Macrophage Phagocytosis ◽

Red Ginger

Red ginger (Zingiber officinale) contains many chemical compounds that have many activities. This study aims to determine the active compounds of Zingiber officinale and immunomodulatory activity that can increase the phagocytosis of macrophage cells and lymphocyte cell proliferation. Red ginger extract compounds were identified using GC-MS. The parameters of immunostimulatory activity were macrophage phagocytosis and lymphocyte proliferation, the preparations were made in several extract concentrations. This research is an experimental study in the laboratory. The research included fresh red ginger, made into ethanol extract, dried. The results were made a suspension, the extract was then given to macrophage cells (phagocytosis testing) and lymphocyte cells (proliferation testing) in vitro to test the immunomodulatory effect. The results showed that the most active extract in macrophage phagocytosis activity was the extract concentration of 500 ppm. While the activity of lymphocyte proliferation was shown by the concentration of group A (Cell, 500 ppm extract, MK, Engerik). The active compound contained in the ethanol extract of red ginger is thought to be Geraniol

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Anatomical aspects of the ginger flower Etlingera elatior (Jack.) RM Smith, acclimatization with the usage of different nutritive solutions

Emirates Journal of Food and Agriculture ◽

10.9755/ejfa.2019.v31.i9.2010 ◽

2019 ◽

pp. 708

Author(s):

Jessé Marques da Silva Júnior Pavão ◽

Evaristo Mauro De Castro ◽

Selenobaldo Alexinaldo Cabral Sant’Anna ◽

Thomé Rodrigues de Pontes Bomfim ◽

A.F. dos Santos, J.G. da Costa, M.A. Souza, T.J.M. Rocha

Keyword(s):

Cross Sections ◽

Stomatal Density ◽

Anatomical Study ◽

Tropical Species ◽

Spongy Parenchyma ◽

Transparent Plastic ◽

Red Ginger ◽

Nutrient Solutions ◽

Etlingera Elatior

The Ginger flower, Etlingera elatior (Jack.) RM Smith (also known as torch ginger, ginger flower, red ginger lily, torch lily, wild ginger), belonging to the Zingiberaceae family, stands out with one of the main ornamental tropical species. The objectives of the present study were to establish an acclimatization methodology for in vitro cultivated cane seedlings, and to compare the internal structure of leaves of seedlings submitted to different nutrient solutions. The seedlings from the in vitro culture were transplanted into trays with inert substrate (expanded vermiculite + 10% sand), where they remained in a growth room with controlled temperature of 25 ± 2 °C and photon irradiance of 67 μm m-2 s-1. Moisture control was carried out by using a transparent plastic bag, which was removed after 15 days and shortly thereafter, the experiments were started with the use of nutritive solutions MS (Murashige & Skoog), BJ (Bolle-Jones) and HO (Hoagland & Arnon) at 35% of their ionic strength. For the anatomical study, transverse and paradermal sections were realized in the foliar laminae of seedlings in vitro, in period of acclimatization and in plants already established. The in vitro seedlings showed survival at 100% when transferred to the ex vitro condition. In the cross sections and leaf parser, anatomical differences between the seedlings of the different environments were observed. The abaxial and adaxial epidermis presented significant differences in the environments in which the seedlings developed, showing themselves to be thicker as the acclimatization period increased. The thickness averages of the palisade parenchyma did not differ statistically in the acclimatization periods and in the different nutrient solutions tested. On the other hand, those ones of the spongy parenchyma´s presented significant differences in relation to the nutrient solutions tested, evidencing in all evaluations the MS solution, which contributed the most to the development of the internal structures of the leaf (spongy parenchyma and central vascular cylinder). The highest stomatal density was observed in seedlings developed in vitro (250 stomata per mm2) when compared to those ones already acclimatized or even to that one of field, 200 and 190 stomata per mm2 respectively. The stomata of the Ginger flower leaves developed in vitro presented with polar and equatorial diameters greater than the stomata of leaves already acclimatized and as well as of field.

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