scholarly journals Synthesis, Characterization and Antimicrobial Study of Vanadium (V) oxide using Alkoxide Precursor

2021 ◽  
Vol 2 (1) ◽  
pp. 1-6
Author(s):  
Dr. Adam Hassan Elhaj Yousif ◽  
Nosaiba Abdalgadir Nusr Mohammed
Keyword(s):  
Clinical Laboratory ◽  
Gelling Agent ◽  
Diffusion Method ◽  
National Committee ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Antimicrobial Study ◽  
Ft Ir ◽  
Ft Ir Spectroscopy

The present study is aimed to synthesize V2O5 using V(ORi)3 precursor and study its anti-microbial activity. The V(ORi)3 was prepared by reaction of VCl3 and isopropanol alcohol. The synthesized V(ORi)3 was hydrolyzed to yield black V(OH)3 gel using concentrated HCl as gelling agent. Yellow V2O5 obtained by thermal treatment of V(OH)3 gel at110oC and 380oC. All products were characterized using FT-IR Spectroscopy method. IR spectral revealed to found V(ORi)3, V(OH)3 gel and V2O5 were formed. The V2O5 has been tested in vitro against numbers of microorganisms, to assess their antimicrobial activity using disc diffusion method, according to the National Committee for Clinical Laboratory Standards Guidelines. The results showed that V2O5 is active against Staphylococcus aureus and Pseudomonas aeruginosa and partial active against Bacillus subtilis, Escherichia coli    and Candida albicans. Antimicrobial Study of VO(OR)3 is required.

TRIALS OF THE DOMESTICALLY PRODUCED NUTRIENT MEDIUM «AGAR MULLER-HINTON II - OBOLENSK»

2019 ◽  
Vol 64 (6) ◽  
pp. 360-367
Author(s):  
I. S. Kosilova ◽  
L. V. Domotenko ◽  
N. K. Fursova ◽  
S. V. Dentovskaya ◽  
M. G. Ershova ◽  
...  
Keyword(s):  
Nutrient Medium ◽  
Antimicrobial Agents ◽  
Clinical Laboratory ◽  
Import Substitution ◽  
Enterobacter Aerogenes ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Bacterial Strains ◽  
Disc Diffusion Method

The results of the comparative tests of the «Agar Muller-Hinton II - Obolensk» nutrient medium developed in SRCAMB, Obolensk, and the control nutrient medium imported «Mueller Hinton II Agar» are presented in the study. The susceptibility of bacterial clinical strains to antimicrobial agents (AMP) was determined by the disc diffusion method and the method of gradient diffusion (E-test). The carbapenemase activity of the strains carrying the carbapenemase genes was determined by CIM-test. Total 173 characterized bacterial strains of species Klebsiella pneumoniae, Pseudomonas aeruginosa, Acinetobacter baumannii, Proteus mirabilis, Serratia marcescens, Enterobacter aerogenes, Escherichia coli; Photorhabdus spp., Staphylococcus aureus, Enterococcus spp. were used in the study, including producers of OXA- and NDM-types carbapenemases for gram negative bacteria. A high degree of coincidence of the results obtained on both nutrient media was shown. The consistency index of the strain sensitivity categories to AMPs (S, I, and R) was 98.2% for the disc diffusion method, and 94.4-100% - for E-test and CIM-test methods. Thus, within the framework of the Import Substitution Program, the domestic nutrient medium «MHA II-Obolensk» has been successfully developed. The nutrient medium meets the requirements of GOST R ISO 20776-2-2010 «Clinical laboratory testing and in vitro diagnostic test systems - Susceptibility testing of infectious agents and evaluation of performance of antimicrobial susceptibility test devices».

scholarly journals Drug sensitivity pattern of Escherichia coli isolated from samples of different biological and environmental sources

1970 ◽  
Vol 6 (1) ◽  
pp. 13-18 ◽  
Author(s):  
MA Zinnah ◽  
MH Haque ◽  
MT Islam ◽  
MT Hossain ◽  
MR Bari ◽  
...  
Keyword(s):  
Drug Sensitivity ◽  
Clinical Laboratory ◽  
Intermediate Phase ◽  
Sensitivity Test ◽  
Diffusion Method ◽  
National Committee ◽  
E Coli ◽  
Sensitivity Pattern ◽  
Human Faces

A total of 100 different E. coli isolates collected from 10 different biological and environmental sources (10 isolates from each source) such as human faces, human urine, cattle, sheep, goat, chicken, duck, pigeon, drain sewage and soil were used for in-vitro drug sensitivity test in the Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh during the period from January to May 2007. Ten different drugs such as Gentamicin (GM), Azithromycin (AZM), Erythromycin (E), Levofloxacin (LVX), Ciprofloxacin (CIP), Tetracycline (TE), Amoxicillin (A), Ampicillin (AP), Nalidixic acid (NA) and Metronidazole (MET) were used in this study. Sensitivity test was carried out by the Kirby-Bauer disc diffusion method as per recommendation of National Committee for Clinical Laboratory Standards and efficacy of a drug was determined by measuring the diameter of the zone of inhibition that results from diffusion of the agent in to the medium surrounding the disc. A high of 80% and 78% E. coli isolates collectively from all the selected sources were sensitive to LVX and CIP respectively, followed by GM (46%), AZM (45%), TE (30%), AP (29%), E (19%), NA (18%) and A (15%). No isolate was sensitive to MET (0%). Incase of resistance, 96% isolates were resistant to MET, followed by A (72%), E (69%), NA (67%), TE (60%), AP (59%), AZM (33%) and GM (32%), CIP (8%) and LVX (5%). A number of isolates showed intermediate reaction to GM (22%), AZM (22%), LVX (15%), NA (15%), CIP (14%), A(13%), AP (12%), E (12%), TE (10%) and MET (4%). This may be an intermediate phase for the conversion of E. coli isolates from sensitive to resistant form. From the research it may be concluded that E. coli infection of different animals and birds and also of human being may be treated effectively with LVX and CIP followed by GM and AZM. Key words: E. coli isolates, levofloxacin, ciprofloxacin, efficacy, resistance DOI = 10.3329/bjvm.v6i1.1332 Bangl. J. Vet. Med. (2008). 6 (1): 13-18

scholarly journals Synthesis of a New Conjugates of Imidazole wih Beta Lactam Moiety and Evaluation of its Expanded Antibacterial Activity

2018 ◽  
Vol 34 (5) ◽  
pp. 2495-2501 ◽  
Author(s):  
Sarmd D. Noori ◽  
Mazin N. Mousa ◽  
Shaker A. N. Al-Jadaan
Keyword(s):  
Antibacterial Activity ◽  
Chemical Structure ◽  
Bacterial Species ◽  
Anaerobic Bacteria ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Beta Lactam ◽  
Disc Diffusion Method ◽  
E Coli ◽  
Ft Ir

Five compounds containing (2,4,5-triphenyl triphenyl-1H-) and azetidinone (beta-lactam) moiety were synthesized. The physical data and yield of synthesized compounds were recorded, the chemical structure of prepared compounds were characterized using FT-IR, 1H-NMR and elemental analysis. The antibacterial activity was evaluated using disc diffusion method that involve tow Gram positive (staph. aureus, E. Faecalis), two Gram negative (E. coli and ‎K. pneumoniae), and one anaerobic bacteria (streptococcus. Pyogen). Different concentration of the prepared compounds has been used, and the obtained result were compared with standard (ceftazidime). Compound (5c) showed the best antibacterial activity against all bacterial species while 5a and 5e does not. Other compounds showed activity against some species.

scholarly journals Essential Oil from Leaves of Lantana canescens and L. lopez-palacii Grown in Colombia

2011 ◽  
Vol 6 (2) ◽  
pp. 1934578X1100600
Author(s):  
Andrés F. Peralta-Bohórquez ◽  
Clara Quijano-Célis ◽  
Mauricio Gaviria ◽  
Consuelo Vanegas-López ◽  
Jorge A. Pino
Keyword(s):  
Essential Oil ◽  
Volatile Compounds ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Enterobacter Sakazakii ◽  
Bacterial Strains ◽  
Disc Diffusion Method ◽  
Germacrene D ◽  
In Vitro Antibacterial Activity

The chemical composition of the volatile compounds from the leaves of Lantana canescens Kunth (Verbenaceae) and L. lopez-palacii Moldenke grown in Colombia were analyzed by GC and GC/MS. One hundred and thirty-nine volatile compounds were identified in L. canescens, of which the major ones were β-caryophyllene (13.5%), germacrene D (10.3%) and 1-octen-3-ol (8.4%). In the oil obtained from L. lopez-palacii, eighty-three compounds were identified, of which the most prominent were 1-octen-3-ol (24.4%) and β-caryophyllene (15.2%). The in vitro antibacterial activity of the L. lopez-palacii essential oil was studied against three bacterial strains using the disc diffusion method. No antimicrobial activity was found against Escherichia coli, Enterobacter sakazakii and Listeria monocytogenes.

In vitro sensitivity of Hungarian Actinobaculum suis strains to selected antimicrobials

2003 ◽  
Vol 51 (1) ◽  
pp. 53-59 ◽  
Author(s):  
I. Biksi ◽  
Andrea Major ◽  
L. Fodor ◽  
Keyword(s):  
Type Strain ◽  
Diffusion Method ◽  
Resistance Pattern ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Antimicrobial Sensitivity ◽  
Variable Sensitivity ◽  
Agar Dilution ◽  
Almost All

In vitro antimicrobial sensitivity of 12 Hungarian isolates and the type strain ATCC 33144 of Actinobaculum suis to different antimicrobial compounds was determined both by the agar dilution and by the disc diffusion method. By agar dilution, MIC50 values in the range of 0.05-3.125µg/ml were determined for penicillin, ampicillin, ceftiofur, doxycycline, tylosin, pleuromutilins, chloramphenicol, florfenicol, enrofloxacin and lincomycin. The MIC50 value of oxytetracycline and spectinomycin was 6.25 and 12.5µg/ml, respectively. For ofloxacin, flumequine, neomycin, streptomycin, gentamicin, nalidixic acid, nitrofurantoin and sulphamethoxazole + trimethoprim MIC50 values were in the range of 25-100µg/ml. With the disc diffusion method, all strains were sensitive to penicillin, cephalosporins examined, chloramphenicol and florfenicol, tetracyclines examined, pleuromutilins, lincomycin and tylosin. Variable sensitivity was observed for fluoroquinolones (flumequine, enrofloxacin, ofloxacin), most of the strains were susceptible to marbofloxacin. Almost all strains were resistant to aminoglycosides but most of them were sensitive to spectinomycin. A strong correlation was determined for disc diffusion and MIC results (Spearman's rho 0.789, p<0001). MIC values of the type strain and MIC50 values of other tested strains did not differ significantly. Few strains showed a partially distinct resistance pattern for erythromycin, lincomycin and ampicillin in both methods.

scholarly journals Synthesis and Antimicrobial Assessment of Fe3+ Inclusion Complex of p-tert-Butylcalix[4]arene Diamide Derivative

2019 ◽  
Vol 2019 ◽  
pp. 1-8 ◽  
Author(s):  
Anwar Ali Chandio ◽  
Ayaz Ali Memon ◽  
Shahabuddin Memon ◽  
Fakhar N. Memon ◽  
Qadeer Khan Panhwar ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Diffusion Method ◽  
Gram Negative Bacteria ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Gram Negative ◽  
Mueller Hinton ◽  
Mueller Hinton Agar ◽  
Ft Ir ◽  
Bacteria And Fungi

Present study deals with the synthesis of the p-tert-butylcalix[4]arene diamide derivative as ligand (L) and its Fe3+ complex, followed by its characterization using TLC and FT-IR, while UV-Vis and Job’s plot study were performed for complex formation. Antimicrobial activity of the derivative (L) and its metal complex was carried out by the disc diffusion method against bacteria (Escherichia coli and Staphylococcus albus) and fungi (R. stolonifer). Different concentrations of the derivative (L) (6, 3, 1.5, 0.75, and 0.37 μg/mL) and its Fe3+ complex were prepared, and Mueller–Hinton agar was used as the medium for the growth of microorganisms. Six successive dilutions of the derivative (L) and Fe3+ complex were used against microorganisms. Two successive dilutions (6 and 3 μg/mL) of the derivative (L) showed antibacterial action against both Gram-positive and Gram-negative bacteria. In addition, three successive dilutions (6, 3, and 1.5 μg/mL) of the derivative (L) showed antifungal activity. However, all of six dilutions of the Fe3+ complex showed antimicrobial activity. Derivative (L) showed 3 and 1.5 μg/mL minimum inhibitory concentrations (MIC) against bacteria and fungi, respectively. On the contrary, its Fe3+ complex showed 0.37 μg/mL value of MIC against bacteria and fungi. Hence, Fe3+ complex of the derivative (L) was found to be a more effective antimicrobial agent against selected bacteria and fungi than the diamide derivative (L).

scholarly journals Synthesis and in vitro antibacterial activity of Cephradine Benzoate

1970 ◽  
Vol 10 (3) ◽  
pp. 148-151
Author(s):  
M Ahmad ◽  
M Rahman ◽  
P Kumar Paul
Keyword(s):  
Antibacterial Activity ◽  
Salmonella Typhi ◽  
Shigella Dysenteriae ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Nmr Studies ◽  
Shigella Boydii ◽  
Group B

Aims & Methods: The present study was undertaken to compare the antibacterial activity of a cephradine derivative with that of the parent antibiotic cephradine. Cephradine was converted to its benzoyl derivative by Schotten-Baumann method for the first time. Disc diffusion method was employed to find out the antibacterial activity against EPEC, ETEC, E. Agg, Salmonella typhi, Salmonella group B, Shigella boydii, Shigella dysenteriae 1, Shigella dysenteriae 2, Shigella flexinariae and Shigella sonnei. Melting point, TLC, HPLC, UV, FTIR and <sup>1</sup>H NMR studies were carried out to check the purity and confirm that the derivative was cephradine benzoate. Results: The benzoyl derivative showed promising activity against tested bacteria. The results obtained from the study demonstrate that the benzoyl derivative could be a potential antibacterial agent. Key words: Cephradine benzoate, antibacterial activity, disc diffusion method.   DOI: http://dx.doi.org/ 10.3329/bjms.v10i3.8356 BJMS 2011; 10(3): 148-151

scholarly journals Studies of Antimicrobial Activity of 2',4',5'- and 2',3',4'-Trimethoxy Flavanones

2016 ◽  
Vol 8 (2) ◽  
pp. 209-216
Author(s):  
A. Rahim ◽  
R. Ali ◽  
A. Islam
Keyword(s):  
Pathogenic Bacteria ◽  
Sclerotium Rolfsii ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Filter Paper Disc ◽  
Antibacterial And Antifungal ◽  
Paper Disc

 2',4',5'- and 2',3',4'-trimethoxy flavanones have been synthesized starting with 2-hydroxyacetophone and substituted aldehyde. Antibacterial activities of the flavanones have been tested along with their corresponding chalcones against two human pathogenic bacteria (Streptococcus-b-haemolyticus and  Klebsiella sp. (G-)). Antifungal activities of the flavanones have also been investigated against  two plants pathogenic mold fungi (Rhizactonia solani Sclerotium rolfsii). The structures of the synthesized compounds have been characterized with the help of  UV, IR and 1H NMR and 13C-NMR spectra. The antibacterial and antifungal screening were performed in vitro by the filter paper disc diffusion method and poisoned food technique. The flavanones showed antibacterial activity while no activity was observed to their corresponding chalcones against the tested bacteria. On the other hand, chalcones and their corresponding flavanones both showed fungicidal activities.

scholarly journals Efek Antimikroba Ekstrak Air Daun Mengkudu (Morinda citrifolia L.) terhadap Bakteri Shigella dysenteriae Secara In Vitro

2021 ◽  
Vol 3 (1) ◽  
pp. 14-18
Author(s):  
Qyana Al Farisi ◽  
Yuke Andriane ◽  
Miranti Kania Dewi
Keyword(s):  
Water Extract ◽  
Antimicrobial Effect ◽  
Negative Control ◽  
Shigella Dysenteriae ◽  
Morinda Citrifolia ◽  
High Price ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method

Kematian akibat infeksi Shigella, terutama Shigella dysenteriae dapat mencapai lebih dari 10% terutama pada anak dan lanjut usia pada kondisi tanpa pemberian terapi yang efektif. Siprofloksasin merupakan lini pertama untuk pengobatan infeksi Shigella, akan tetapi obat ini memiliki beberapa kekurangan di antaranya harga yang mahal dan resistensi. Daun mengkudu merupakan tanaman tradisional yang diduga memiliki efek antimikro dan diharapkan dapat menjadi alternatif terapi antibiotik bagi Shigella dysenteriae yang saat ini sudah banyak mengalami resistensi. Tujuan penelitian ini adalah mengetahui efek antimikro ekstrak air daun mengkudu (Morinda citrifolia L.) terhadap bakteri Shigella dysenteriae. Penelitian ini bersifat eksperimental laboratorium murni. Zona hambat ditentukan melalui metode difusi cakram. Objek penelitian yang digunakan adalah Shigella dysenteriae ATCC nomor 13313. Sampel uji berupa ekstrak air daun mengkudu konsentrasi 100%, kontrol positif (siprofloksasin) dan kontrol negatif (aquadest) dengan 9 kali pengulangan. Hasil uji antimikro dengan metode difusi cakram menunjukkan tidak terbentuk zona hambat pada ekstrak air daun mengkudu konsentrasi 100%. Hal tersebut menunjukkan ekstrak air daun mengkudu konsentrasi 100% tidak memiliki efek antimikro terhadap bakteri Shigella dysenteriae. Kadar flavonoid, tanin, dan alkaloid yang terdapat dalam ekstrak air daun mengkudu konsentrasi 100% pada penelitian ini kemungkinan belum cukup untuk menghasilkan efek yang diharapkan. Antimicrobial Effect of Water Extract of Noni (Morinda citrifolia L.) Leaves against Shigella dysenteriae In VitroDeath due to infection with Shigella, especially Shigella dysenteriae, can reach more than 10%, especially in children and the elderly in conditions without effective therapy. Ciprofloxacin is the first line for the treatment of Shigella infection, however this drug has several disadvantages including high price and resistance. Noni leaf is a traditional plant that is thought to have antimicrobial effects and is expected to be an alternative antibiotic therapy for Shigella dysenteriae which is currently experiencing a lot of resistance. The purpose of this study was to determine the antimicrobial effect of the water extract of noni leaves (Morinda citrifolia L.) on Shigella dysenteriae bacteria. This research is a pure laboratory experimental study. The zone of inhibition is determined by the disc diffusion method. The research object used was Shigella dysenteriae ATCC number 13313. The test sample was a water extract of noni leaves with a concentration of 100%, positive control (ciprofloxacin) and negative control (aquadest) with 9 repetitions. The results of the antimicrobial test using the disc diffusion method showed no inhibition zone was formed in the water extract of noni leaves with a concentration of 100%. This shows that a water extract of noni leaves with a concentration of 100% did not have an antimicrobial effect against the Shigella dysenteriae bacteria. The levels of flavonoids, tannins, and alkaloids contained in the water extract of noni leaves with a concentration of 100%  in this study may not be sufficient to produce the expected effect.

scholarly journals IN VITRO ASSESSMENT OF ANTIBACTERIAL ACTIVITY OF STEM AND ROOT OF RAUWOLFIA SERPENTINA AGAINST DIFFERENT BACTERIA

2021 ◽  
Vol 12 (6) ◽  
pp. 87-90
Author(s):  
Virendra Vaishnav ◽  
Debasish Sahoo ◽  
Tanushree Chatterjee
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Antimicrobial Agents ◽  
Development Program ◽  
Positive Control ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Rauwolfia Serpentina

Medicinal Plants are the good source of natural antimicrobial agents. The main aim of present study was to evaluate the antibacterial activity of stem and root of Rauwolfia serpentina against six microorganism, Powdered stem and root of plant were extracted with acetone, chloroform and methanol and streptomycin used as positive control. The antibacterial activity of Rauwolfia serpentine was detected by using disc diffusion method and agar well diffusion method on the following bacteria- Bacillus cereus, Staphylococcus aureus, Bacillus fusiformis, Escherichia coli, Pseudomonas aeruginosa and P. luminescens. The experiment reported that R. serpentina Root methanol extract shown 14.86 ± 1.11 highest antibacterial activity against Pseudomonas aeruginosa through well diffusion method. Whereas root chloroform recorded 13.46 ± 1.28 highest antibacterial activity against E. coli through disc diffusion method, maximum zone of inhibition 22.66±0.52 mm was found for the positive control, streptomycin through well diffusion method. Further studies should be undertaken to reveal the correct mechanism of action of antimicrobial effect to identify the active ingredients which can be used in drug development program.

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