scholarly journals Hepatoprotective Activity of Leaves Extract of Bauhinia acuminata (Linn) against CCl4 Induced Hepatotoxicity in Albino Rats

2021 ◽  
Vol 69 (1) ◽  
Author(s):  
Mohsin J. Jamadar ◽  
Preeti Khulbe ◽  
Shrinivas K. Mohite
Keyword(s):  
Antioxidant Enzymes ◽  
Biochemical Parameters ◽  
Low Dose ◽  
Ethanolic Extract ◽  
Hepatoprotective Activity ◽  
Albino Rats ◽  
High Dose ◽  
Treated Animal ◽  
Aqueous Extracts ◽  
Hepatic Lipid Peroxidation

The purpose of this study was to see if ethanolic and aqueous extracts of Bauhinia acuminatea (Linn.) had any hepatoprotective activity against CCl4-induced albino rats. The levels of biochemical parameters (SGOT, SGPT and ALP) were reduced and the levels of hepatic antioxidant enzymes such as GSH and CAT were decreased whereas the level of hepatic lipid peroxidation (MDA) was elevated byCCl4 induction in Albino rats when compared with the normal group. The ethanolic and aqueous extract of Bauhinia acuminata (Linn.) and Silymarin treated animal groups showed significant decrease in activities of different biochemical parameters like SGOT, SGPT, ALP and lipid peroxidase i.e., MDA level which were elevated by carbon tetrachloride (CCl4) intoxication, at doses of 200 and 400 mg/kg and increased the level of antioxidant enzymes such as GSH and CAT. The high dose of ethanolic extracts (400 mg/kg) was more effective as compared to low dose (200 mg/kg). So, it was concluded from the result that the ethanolic extract of Bauhinia acuminata (Linn.) possesses significant hepatoprotective activity compared to aqueous extracts against CCl4 induced hepatotoxicity in rats.

scholarly journals Hepatoprotective activity of the ethanolic extract of Morus indica roots from Indian Bodo tribes

2022 ◽  
Vol 4 (2) ◽  
Author(s):  
Hankhray Boro ◽  
Talambedu Usha ◽  
Dinesh Babu ◽  
Prakashmurthy Chandana ◽  
Arvind Kumar Goyal ◽  
...  
Keyword(s):  
Antioxidant Enzymes ◽  
Ethanolic Extract ◽  
Hepatoprotective Activity ◽  
Positive Control ◽  
Active Components ◽  
Traditional Use ◽  
Hematological Indices ◽  
Pharmacological Mechanism ◽  
Hepatic Lipid Peroxidation

AbstractThe roots of Morus species are well described in the Pharmacopoeia of the People's Republic of China (ChP) for its traditional use in treating liver fibrosis due to its hepatoprotective property. However, little is known about the hepatoprotective effect of the roots of Morus indica L. (RoMi), and the pharmacological mechanism(s) are uncertain due to its intricacy. Therefore, this study evaluates the hepatoprotective activity of the ethanolic extract of RoMi (eRoMi) against the CCl4-induced in-vivo animal model at different dosages (100 and 200 mg/kg BW) in comparison with silymarin as a positive control. The hepatoprotective activity of eRoMi was evaluated by measuring the levels of serum biomarkers, hepatic antioxidant enzymes and was verified by histological studies. Interestingly, 1,2-bis(trimethylsilyl) benzene, 1,4-phenylenebis (trimethylsilane), 2,4,6-cycloheptatriene-1-one, 3,5-bis-trimethylsilyl and α-amyrin were the active components found in eRoMi as detected by GC–MS. Oral administration of eRoMi (200 mg/kg BW) to rats significantly protected serum biochemical parameters (increased ALT, AST, LDH, bilirubin and GGT as well as depletion of antioxidant enzymes and hepatic GSH) and elevation in hepatic lipid peroxidation as compared to CCl4-treated rats. The hematological indices such as erythrocytes, hemoglobin, monocytes and lymphocytes were also normal in eRoMi-treated rats. The histopathological evaluation indicated a significant restoration of liver structure as compared to silymarin. This study is the first scientific validation for the traditional use of eRoMi to understand its hepatoprotective activity.

Assessment of hepatoprotective activity of coded plant (222) leaf ethanolic extract against carbon tetrachloride-induced hepatotoxicity in Wistar rats – Part IV

2018 ◽  
Vol 5 (2) ◽  
Author(s):  
Krishnakumar N M
Keyword(s):  
Carbon Tetrachloride ◽  
Biochemical Parameters ◽  
Subcutaneous Administration ◽  
Ethanolic Extract ◽  
Treated Group ◽  
Hepatoprotective Activity ◽  
Control Group ◽  
Albino Rats ◽  
Hepatic Enzymes ◽  
Standard Drug

The present study was designed to assess the possible hepatoprotective activity of the leaf ethanolic extract of coded plant (Code No. 222**) against carbon tetrachloride (CCl4)-induced hepatic injury in Wistar albino rats. The animals were divided into different groups and treated with 222 leaf ethanolic extract at different concentrations for five days. Silymarin, the known hepatoprotective standard compound (100 mg/kg) was administered for five days. Hepatotoxicity was induced by the subcutaneous administration of a single dose of CCl4: Olive oil (2 mL/kg) on days 2 and 3. The administration of CCl4 resulted in marked increase in serum hepatic enzymes such as alanine aminotransferase (ALT), aspartate aminotransferase (AST) and serum bilirubin levels. CCl4 intoxication also resulted in a significant (P=0.05) increase in malondialdehyde (MDA), which is a common marker of lipid peroxidation. The other biochemical parameters such as cholesterol, triglycerides, creatinine, urea and uric acid levels were also increased significantly (P=0.05) compared to normal control group. Changes in serum hepatic enzymes, biochemical parameters and MDA levels induced by CCl4 were reversed by the leaf ethanolic extract of 222 (125 mg/kg) dose. The standard drug silymarin treated group also reversed CCl4-induced changes in biomarkers of liver function and MDA levels. Histopathological studies of the liver samples confirmed the hepatoprotective property of the coded drug 222. It was seen that histopathological damage induced by CCl4 were improved in rat liver, treated with 222 extract. The results of the present study suggested that coded plant (222) leaf ethanolic extract may be used as a hepatoprotective agent against toxic effects caused carbon tetrachloride in the liver.

scholarly journals Evaluation of in vivo Hepatoprotective activity of Mimosa rubicaulis (LAM.) against CCL4 Induced Liver Toxicity

2021 ◽  
Vol 69 (1) ◽  
Author(s):  
Ashpak M. Tamboli ◽  
Kiran A. Wadkar
Keyword(s):  
Antioxidant Enzymes ◽  
Wistar Rats ◽  
Liver Toxicity ◽  
Chemical Constituents ◽  
Ethanolic Extract ◽  
Hepatoprotective Activity ◽  
Treated Animal ◽  
Ccl4 Administration ◽  
Female Wistar Rats

The current study was conducted to assess the hepatoprotective activity of different extracts of M. rubicaulis (Lam.) at (200 and 400 mg/kg) doses b.w. against CCl4 induced liver intoxication in Albino Wistar rats. Male or female Wistar rats about 150 and 200 gm body weight were selected for present study. The animals were divided into thirteen groups, six rats in each group. The extracts and Silymarin-treated animal groups significantly reduced the activities of various biochemical markers such as SGPT, SGOT, ALP, and TB which were elevated by carbon tetrachloride (CCl4) intoxication. The extracts of M. rubicaulis (Lam.) showed a dose dependent hepatoprotection activity. Among all the extracts, ethanolic extract produced maximum hepatoprotection (SGPT-83.15 %, SGOT76.82%, ALP-79.33%, TB-80.00%) at a 400mg/kg dose. After CCl4 administration, the levels of hepatic-antioxidant enzymes such as Glutathione (GSH) and Catalase (CAT) were reduced, whereas the level of hepatic lipid peroxidation (-LPO) increased. These hepatic antioxidant enzymes were also restored to normal levels by extracts and Silymarin treatment. The results of the present investigation indicate that all the extracts of M. rubicaulis (Lam.) possess hepatoprotective activity which may be due to the presence of various chemical constituents.

scholarly journals Hepatoprotective activity of Cuscuta reflexa aqueous and alcoholic extracts against CCl4 induced toxicity in rats

2020 ◽  
pp. 463-466
Author(s):  
Rakesh RANJAN ◽  
Manoj KUMAR ◽  
Amar KUMAR ◽  
Manoranjan Prasad SINHA
Keyword(s):  
Low Dose ◽  
Total Bilirubin ◽  
Hepatoprotective Activity ◽  
Albino Rats ◽  
High Dose ◽  
Alcoholic Extract ◽  
Cuscuta Reflexa ◽  
Ccl4 Administration ◽  
Altered Liver ◽  
The Impact

Introduction In the present study the impact of aqueous and alcoholic extracts of Cuscuta reflexa were investigated on rats intoxicated with carbon tetrachloride (CCl4). CCl4 is known to intoxicate the liver of rats which can be easily be observed by examining the total protein, total bilirubin, alkaline phosphatase (ALP), aspartate aminotransferase (AST), alanine transaminase ALT). Cuscuta reflexa have been used in traditional medicine culture from time immemorial. In this study both the aqueous and alcoholic extracts of Cuscuta reflexa were found to have curative impact on liver profile of CCl4 intoxicated rats. Materials and Methods. The stem of Cuscuta reflexa were collected from host bougainvillea, dried in shade and were subjected to alcoholic (ethanolic), and aqueous extraction. Albino rats were intoxicated with CCl4 to induce hepatotoxicity. The CCl4 intoxicated rats were treated with low dose and high dose of both the extracts to assess the hepatoprotective impact on intoxicated rats. The results clearly revealed that the CCl4 administration altered liver profile. The altered liver profile parameters recovered to normal after administration of aqueous and alcoholic extracts. Results and Discussion. Administration of CCl4 induced hepatotoxicity in albino rats, which was evident with the results of serum analysis. Post CCl4 administration the liver profile parameters were altered. The CCl4 intoxicated rats were then treated with aqueous and alcoholic extracts of Cuscuta reflexa. Post treatment with the extracts the liver profile parameters recovered to normal. Conclusion: Present study reveals that aqueous and alcoholic extracts of Cuscuta reflexa were found to have curative impact on liver profile of CCl4 intoxicated rats. Keywords: Custuca reflexa, CCl4, hepatoprotective, liver profile, alcoholic extract, aqueous extract,

scholarly journals Protective Effect of Ethanolic Extract ofTabernaemontana divaricata(L.) R. Br. against DEN and Fe NTA Induced Liver Necrosis in Wistar Albino Rats

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Kannappan Poornima ◽  
Palanisamy Chella Perumal ◽  
Velliyur Kanniappan Gopalakrishnan
Keyword(s):  
Ethanolic Extract ◽  
Treated Group ◽  
Hepatic Necrosis ◽  
Hepatoprotective Activity ◽  
Albino Rats ◽  
Protective Effects ◽  
Liver Necrosis ◽  
Standard Drug ◽  
Tabernaemontana Divaricata ◽  
Wistar Albino Rats

This study is an attempt to evaluate the hepatoprotective activity ofTabernaemontana divaricataagainst DEN and Fe NTA induced liver necrosis in rats. Ethanolic extract of the whole plant ofTabernaemontana divaricataat doses of 200 and 400 mg/kg body weight and 5-fluorouracil (standard drug) was orally administered to male Wistar Albino rats once daily for 24 weeks, simultaneously treated with the carcinogen DEN and Fe NTA. In simultaneously treated animals, the plant extract significantly decreased the levels of uric acid, bilirubin, AST, ALT, and ALP in serum and increased the levels of liver marker enzymes in liver. Treatment with the extracts resulted in a significant increase in the levels of antioxidants accompanied by a marked reduction in the levels of malondialdehyde when compared to DEN and Fe NTA treated group. When compared with 200 mg/kg bw rats, 400 mg/kg bw rats and 5-fluorouracil treated rats showed better results in all the parameters. The histopathological studies confirmed the protective effects of extract against DEN and Fe NTA induced liver necrosis. Thus, it could be concluded that the use ofTabernaemontana divaricataextract in the treatment of carcinogen induced hepatic necrosis.

scholarly journals Anti-Fatigue Activity of Aqueous Extracts of Sonchus arvensis L. in Exercise Trained Mice

Molecules ◽  
2019 ◽  
Vol 24 (6) ◽  
pp. 1168 ◽  
Author(s):  
Tian Yuan ◽  
Di Wu ◽  
Keyu Sun ◽  
Xintong Tan ◽  
Jia Wang ◽  
...  
Keyword(s):  
Low Dose ◽  
Glycogen Synthesis ◽  
High Dose ◽  
Aqueous Extracts ◽  
Mediating Effects ◽  
Physical Fatigue ◽  
Gene Expressions ◽  
Muscle Structure ◽  
Swimming Test ◽  
Sonchus Arvensis

Sonchus arvensis L. is a nutritious vegetable and herbal medicine that is consumed worldwide. The aim of this study was to evaluate the anti-fatigue effects and underlying effects of aqueous extract of Sonchus arvensis L. (SA). Male C57BL/6 mice from four groups designated vehicle, exercise, exercise with low dose (250 mg/kg) or high dose of SA (500 mg/kg), were trained by swimming exercise and orally administrated with SA every other day for 28 days. The anti-fatigue activity was determined by exhaustive swimming test, as well as the muscle structure, levels of blood hemoglobin, and metabolites including lactate and urea nitrogen. SA alleviated mice fatigue behaviors by eliminating metabolites, while improving muscle structure and hemoglobin levels. Moreover, SA enhanced glycogen synthesis of liver but not muscle via increasing GCK and PEPCK gene expressions. Importantly, SA improved antioxidant enzymes expression and activities in both liver and muscle, which was possibly related to its primary components polysaccharides and the antioxidant components including chlorogenic acid, luteolin, and chicoric acid. Taken together, the anti-fatigue effects of SA could be partly explained by its antioxidant activity and mediating effects on glycogen synthesis and metabolites elimination. Therefore, SA could be a potential nutraceutical for improving exercise performance and alleviating physical fatigue.

scholarly journals Hepatoprotective effect of aqueous extract of cardamom against gentamicin induced hepatic damage in rats

2015 ◽  
Vol 5 (1) ◽  
pp. 1 ◽  
Author(s):  
Mohamed Aboubakr ◽  
Abdelazem Mohamed Abdelazem
Keyword(s):  
Aqueous Extract ◽  
Histopathological Examination ◽  
Low Density Lipoprotein ◽  
Elevated Serum ◽  
Density Lipoprotein ◽  
Serum Levels ◽  
Hepatoprotective Activity ◽  
Lipoprotein Cholesterol ◽  
Albino Rats ◽  
Aqueous Extracts

<p>The study was designed to evaluate the hepatoprotective activity of aqueous extract of cardamom in acute experimental liver injury induced by gentamicin. Twenty four male albino rats were randomly divided into four groups (six rats in each). Animals of the first group served as control and orally (p.o.) received (1 ml/kg saline). The second experimental group was given gentamicin (80 mg/kg i.p.) for 7 days. Third and fourth groups were given aqueous extract of cardamom (100 and 200 mg/kg p.o.) + gentamicin for 7 days, respectively. The degree of hepatoprotection was measured using serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), bilirubin, albumin, and lipid profile levels. In the acute liver damage induced by gentamicin, cardamom aqueous extracts (100 and 200 mg/kg, p.o.) significantly reduced the elevated serum levels of AST, ALT, bilirubin, cholesterol, triglycerides and low density lipoprotein cholesterol (LDL-chol) in gentamicin induced hepatotoxicity. Also cardamom aqueous extracts (100 &amp; 200 mg/kg, p.o.) significantly increased the lowered serum levels of albumin and high density lipoprotein cholesterol (HDL-chol) in gentamicin induced hepatotoxicity rats. Histopathological examination of the liver tissues supported the hepatoprotection. Our findings concluded that cardamom aqueous extracts possessed hepatoprotective activity against gentamicin induced hepatotoxicity in rats.</p>

scholarly journals Effect of Marine Phytoplankton (Oceanix) and Undenatured Type II Collagen in Combination with Exercise on Endurance Capacity and Oxidative Stress in Rats

2020 ◽  
Vol 4 (Supplement_2) ◽  
pp. 1764-1764
Author(s):  
Kazim Sahin ◽  
Cemal Orhan ◽  
Mehmet Tuzcu ◽  
Nurhan Sahin ◽  
Vijaya Juturu
Keyword(s):  
Antioxidant Enzymes ◽  
Proinflammatory Cytokines ◽  
Type Ii Collagen ◽  
Marine Phytoplankton ◽  
Albino Rats ◽  
High Dose ◽  
Endurance Capacity ◽  
Type Ii ◽  
Tnf Α ◽  
Undenatured Type Ii Collagen

Abstract Objectives To study the effect of exercise training alone and or in combination with marine phytoplankton (Oceanix, OCX) and undenatured type II collagen (UCII) supplementation on the endurance capacity, pro-inflammatory markers, and antioxidant defense markers in rats. Methods A total of 28 male Wistar albino rats were randomly divided into four groups (n  =  7) (i) No exercise and no OCX (Control), (ii) Exercise, (iii) Exercise +OCX-I (2.55 mg d/rat) + UC-II (4 mg), iv) Exercise + OCX-2 (5.1 mg d/rat)+UC-II (4 mg). Levels of cholesterol, triglyceride, proinflammatory cytokines (IL-1β, IL-6, TNF-α, COMP, CRP), lactate and malondialdehyde (MDA) levels activities of antioxidant enzymes were determined in all the groups. Results Run to exhaustion (minutes) improved in the OCX + UC-II treated groups. Levels of cholesterol, triglyceride, proinflammatory cytokines (IL-1β, IL-6, TNF-α, COMP, CRP) decreased by OCX + UC-II supplementation. A significant decrease in lactate and malondialdehyde (MDA) levels and an increase in activities of antioxidant enzymes were observed in the combination of exercise and OCX + UC-II groups. Exercise + OCX + UC-II treated had lower TNF-α and IL-1β levels in muscle than exercise and control rats (P &lt; 0.001). Muscle sterol regulatory element-binding protein 1c (SREBP-1c), liver X receptors (LXR), ATP citrate lyase (ACLY) and fatty acid synthase (FAS) levels in the exercise + OCX + UC-II group were lower than all groups (P &lt; 0.05). The effectiveness of the high dose of OCX was more pronounced than the low dose of OCX. Conclusions These results suggest OCX and UC-II with exercise may enhance lipid metabolism by regulation of gene products involved in lipid and antioxidant metabolism including SREBP-1c, -γ, LXR, ACLY and FAS in rats. Funding Sources Lonza.

scholarly journals Secondary Metabolite Profiling, Anti-Inflammatory and Hepatoprotective Activity of Neptunia triquetra (Vahl) Benth

Molecules ◽  
2021 ◽  
Vol 26 (23) ◽  
pp. 7353
Author(s):  
Nasir Aziz Wagay ◽  
Shah Rafiq ◽  
Mohammad Aslam Rather ◽  
Younas Rasheed Tantray ◽  
Feng Lin ◽  
...  
Keyword(s):  
Total Protein ◽  
Ethanolic Extract ◽  
Liver Weight ◽  
Hepatoprotective Activity ◽  
Experimental Models ◽  
Inflammatory Activity ◽  
Albino Rats ◽  
Total Lipids ◽  
Anti Inflammatory

The present study aimed to analyze the phytoconstituents of Neptunia triquetra (Vahl) Benth. Anti-inflammatory and hepatoprotective activities of ethanol (EE), chloroform (CE) and dichloromethane (DCME) of stem extracts were evaluated using in vivo experimental models. The extracts were analyzed for phytoconstituents using GC-HRMS. Anti-inflammatory activity of CE, EE and DCME was accessed using carrageenan-induced paw oedema, cotton pellet-induced granuloma and the carrageenan-induced air-pouch model in Wistar albino rats. The hepatotoxicity-induced animal models were investigated for the biochemical markers in serum (AST, ALT, ALP, GGT, total lipids and total protein) and liver (total protein, total lipids, GSH and wet liver weight). In the in vivo study, animals were divided into different groups (six in each group) for accessing the anti-inflammatory and hepatoprotective activity, respectively. GC-HRMS analysis revealed the presence of 102 compounds, among which 24 were active secondary metabolites. In vivo anti-inflammatory activity of stem extracts was found in the order: indomethacin > chloroform extract (CE) > dichloromethane extract (DCME) > ethanolic extract (EE), and hepatoprotective activity of stem extracts in the order: CE > silymarin > EE > DCME. The results indicate that N. triquetra stem has a higher hepatoprotective effect than silymarin, however the anti-inflammatory response was in accordance with or lower than indomethacin.

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