scholarly journals NORMOGLYCAEMIC EFFECTS OF AQUEOUS EXTRACT OF Parkia biglobosa LEAVES IN ALLOXAN-INDUCED DIABETIC RATS

2020 ◽  
Vol 5 (3) ◽  
pp. 23-39
Author(s):  
I. R. HASSAN, ◽  
A.A Adesokan ◽  
O.T. Odeyemi
Keyword(s):  
Aqueous Extract ◽  
Serum Proteins ◽  
Negative Control ◽  
Diabetic Rats ◽  
Antidiabetic Activity ◽  
Control Group ◽  
Albino Rats ◽  
Phytochemical Analysis ◽  
Parkia Biglobosa ◽  
Traditional Use

Background: Diabetes mellitus is a global health problem leading to an increase in the search for herbal normoglycaemic agents as alternative to the synthetic ones. Aqueous extract of Parkia biglobosa leaves was assessed for normoglycaemic effects in alloxan-induced diabetic rats. The study aim at providing scientific evidence to authenticate the traditional use of Parkia biglobosa leaves in the treatment of diabetes. Methodology: The plant was extracted using aqueous to obtain Parkia biglobosa Leaf Extract (PbLE), qualitative phytochemical analysis was determined using standard methods. Diabetes was induced in albino rats by intraperitoneal injection of 5% solution of alloxan (150 mg/kg bw). The rats were grouped into 5 groups (A, B, C, D and E) of 5 animals each. Group A consisted of non-diabetic rats which served as the control, Group B consisted of diabetic rats that were left untreated and served as negative control, Group C were given glucophage (reference) at a dose level of 7 mg/kg bw, Groups D and E were administered PbLE at the doses of 250 and 500 mg/kg bw respectively. Results: The glucose levels in the blood of rats were checked with a glucometer using the blood from the tail of the rats. Serum (proteins, lipid profiles, urea and creatinine), ALT, AST and ALP were all determined using standard procedures. The extract and the glucophage reduced the blood glucose level significantly (p < 0.05) from day 3 till the termination of the experiment.                                                                                                                                                Conclusion: Aqueous extract of Parkia biglobosa leaves possess antidiabetic activity and also the extract is relatively safe.  Hence the leaves of Parkia biglobosa can be explored in producing alternative antidiabetic drugs.

scholarly journals Comparison between the Antioxidant and Antidiabetic Activity of Fenugreek and Buckthorn in Streptozotocin-Induced Diabetic Male Rats

2021 ◽  
Vol 2021 ◽  
pp. 1-12
Author(s):  
Mohammed A. Alsieni ◽  
Haddad A. El Rabey ◽  
Abdulbasit I. Al-Sieni ◽  
Madeha N. Al-Seeni
Keyword(s):  
Antioxidant Activity ◽  
Aqueous Extract ◽  
Density Lipoprotein ◽  
Negative Control ◽  
Seed Extract ◽  
Male Rats ◽  
Antidiabetic Activity ◽  
Control Group ◽  
Albino Rats ◽  
Leaf Aqueous Extract

This study is aimed at comparing the antidiabetic and antioxidant potential of fenugreek and buckthorn which are commonly used in modulating diabetes in the Middle East. In this study, the antioxidant and antidiabetic activity of the aqueous extracts of the leaf and seed of fenugreek and buckthorn was tested in streptozotocin-induced diabetic male rats fed with a fat-rich diet for 8 weeks. Thirty-six male albino rats were divided into 6 groups ( n = 6 ); the 1st group was the negative control. Diabetes was induced in the other 30 rats using streptozotocin, which were then divided into 5 groups; the 2nd was the untreated positive diabetic group, the 3rd was treated with fenugreek leaf aqueous extract, the 4th was treated with the fenugreek seed aqueous extract, the 5th was treated with buckthorn leaf aqueous extract, and the 6th was treated with buckthorn seed aqueous extract. The positive control group showed an increase in blood sugar, glycated hemoglobin, liver function enzymes, lactate dehydrogenase, kidney indices, total cholesterol, triglycerides, low- and very-low-density lipoprotein, immunoglobulins, and lipid peroxidation and a decrease in high-density lipoprotein, albumin, and antioxidant activity. The histology of the liver and testes showed severe histopathological alterations. Rats of groups 4-6 that were treated with the aqueous extract of the leaf and seed extract of fenugreek and buckthorn showed improvement of all biochemical and histopathological parameters. The seed extract of fenugreek and buckthorn showed more antioxidant activity than their leaves.

scholarly journals IN VITRO ANTIOXIDANT AND IN VIVO ANTIDIABETIC ACTIVITY OF TWO POTENTIAL PROBIOTIC ENTEROCOCCUS SPP. ON ALLOXAN-INDUCED DIABETIC RATS

2021 ◽  
pp. 94-98
Author(s):  
KAMNI RAJPUT ◽  
RAMESH CHANDRA DUBEY
Keyword(s):  
Diabetic Rats ◽  
Antidiabetic Activity ◽  
Control Group ◽  
Albino Rats ◽  
Bacterial Cells ◽  
Bacterial Strains ◽  
Animal Group ◽  
Enterococcus Spp

Objective: In vitro antioxidant activity, in vivo antidiabetic property and intestinal attachment by two potential probiotic bacterial strains, namely, Enterococcus faecium and Enterococcus hirae were studied using albino rats. Methods: Antioxidant the activity was assessed using 2,2-Diphenyl-1-picrylhydrazyl radicals scavenging assay. Alloxan was administered intraperitoneally to induce diabetic conditions in experimental rats. Animals were treated with oral administration of Enterococcus spp., such as E. faecium, and E. hirae isolated from goat and sheep milk. The control animal group received normal saline for the same days. Glibenclamide drug was used as a positive control against probiotic bacterial cells. Results: However, administration of probiotic bacterial strains E. faecium and E. hirae, in albino rats significantly (p<0.05) at varying doses lowered blood glucose levels in diabetic rats as compared to the diabetic control group. Both the species of Enterococcus increased the bodyweight of experimental rats. However, E. faecium was the best antidiabetic strain having the antioxidant activities also in comparison to E. hirae. The attachment of probiotic bacterial cells E. faecium on the rat’s intestine wall against pathogens was examined. Furthermore, E. faecium showed its aggregation with pathogens by attachment of the intestines of albino rats. This showed that both the bacterial strains exhibited in vivo antidiabetic effect. Conclusion: The results of this study showed that probiotic bacteria possess antioxidant, antidiabetic activities, and attachment of intestine.

scholarly journals Antihyperglycemic and Antihyperlipidemic of Karala (Momordica charantia) Fruits in Streptozotocin Induced Diabetic Rats

2012 ◽  
Vol 5 (1) ◽  
pp. 29-37 ◽  
Author(s):  
MA Hossain ◽  
M Mostofa ◽  
D Debnath ◽  
AKMR Alam ◽  
Z Yasmin ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Body Weight ◽  
Total Cholesterol ◽  
Aqueous Extract ◽  
Diabetic Rats ◽  
Momordica Charantia ◽  
The Body ◽  
Control Group ◽  
Albino Rats ◽  
Higher Doses

To investigate the antihyperglycemic and antihyperlipidemic effect of Momordica charantia (Karala), the aqueous extract of the Karala fruit was tested on streptozotocin (STZ)-induced diabetic rats. Thirty six albino rats were used in the experiment, 30 diabetic and the remaining six as negative control (T1). Diabetes was induced by administering (injecting) STZ at dose of 55mg/kg body weight. Thirty diabetic animals were randomly divided into five groups such as diabetic control group (T2) without any application of treatment, and groups T3,T4,T5 and T6 were treated with aqueous extract of Karala fruits daily at the doses of 250,    500 and 750mg/kg and glibenclamide (at a dose of 5mg/kg body weight) respectively. The body weight was taken and blood samples were collected from individual animal to determine glucose levels at 15 day interval up to 90 days. In addition, Asparate  Transaminenase(AST), Alanine Transaminenase(ALT), Alkaline Phosphatase(ALP), Total cholesterol (TCh) and Triglyceride (TGA) were determined at day 15 and at the end of the experiment. All three doses of Karala extracts reduced diabetic induced blood sugar and the reduction is comparable with standard glibenclamide (GLM) dose particularly with higher doses Karala extracts (500 and 750mg). Karala also prevented body weight loss due to induced diabetes as did by GLM treatment.. The treatment also resulted in a significant reduction of Asparate Transaminenase(AST), Alanine Transaminenase(ALT), Alkaline Phosphatase(ALP), Total cholesterol (TCh) and Triglyceride (TGA) activities of treated rats when compared to the STZ induced  diabetic rats. Higher doses of Karala (500 and 750mg/kg) are as effective as standard GLM dose on measured variables. This study demonstrated that Karala has hyperglycemia and antihyperlipidemic effect against STZ induced diabetic rats. These findings open the possibility of using Karala extract to treat diabetic animal and human patients although further research is warranted. DOI: http://dx.doi.org/10.3329/jesnr.v5i1.11550 J. Environ. Sci. & Natural Resources, 5(1): 29 - 37, 2012  

scholarly journals Evaluation the Effect of Vernonia amygdalina. Del Leaves Ethanol Extract on Blood Sugar and its Synergism with Glibenclamide in Rats

2020 ◽  
Vol 13 (1) ◽  
pp. 26-34
Author(s):  
Igwe K ◽  
Ikpeazu O ◽  
Otuokere I
Keyword(s):  
Blood Glucose ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Diabetic Rats ◽  
Antidiabetic Activity ◽  
Control Group ◽  
Vernonia Amygdalina ◽  
Treatment Groups ◽  
Glucose Levels

Antidiabetic activity of Vernonia amygdalina and its possible synergism with glibenclamide was checked. Forty eight rats were used for the research, for hypoglycermic study of V. amygdalina alone, they were grouped into five of six rats each. Group 1 was the negative control and was administered distilled water orally. Groups 2, 3, and 4 were the treatment groups which received 100, 200 and 300 mg/kg body weight of the V. amygdalina extract respectively orally by intubation. Group 5 was the positive control group which received a known antidiabetic drug, glibenclamide. Diabetes was induced with alloxan. For the synergism study, another 18 rats grouped into 3 of six rats each was used. Both groups of glibenclamide only and glibenclamide plus V. amygdalina extract were dosed for 14 days orally by intubation, thereafter were sacrificed and blood collected from heart for analysis. There were 5 replicates grouped by weight throughout the study and both single and synergistic studies had the same controls. Effect of V. amygdalina extract was checked on blood glucose and its possible synergism with glibenclamide. All results in treatment groups were compared with the normal control at statistical confidence of p<0.05. Result shows that V. amygdalina extract reduced blood glucose level in the test groups as dose of extract increased. Combination of V. amygdalina with glibenclamide demonstrated further deduction in blood glucose levels in the treatment rats groups. Therefore addition of V. amygdalina into glibenclamide increased efficacy in the diabetic rats. The interaction between V. amygdalina and glibenclamide in this work was additive and therefore synergistic.

An aqueous extract fromMoringa oleiferaleaves ameliorates hepatotoxicity in alloxan-induced diabetic rats

2017 ◽  
Vol 95 (4) ◽  
pp. 524-530 ◽  
Author(s):  
Mabrouk Attia Abd Eldaim ◽  
Ahmed Shaban Abd Elrasoul ◽  
Samy Ahmed Abd Elaziz
Keyword(s):  
Gene Expression ◽  
Lipid Peroxidation ◽  
Aqueous Extract ◽  
Caspase 3 ◽  
Diabetic Rats ◽  
Control Group ◽  
Albino Rats ◽  
Hepatic Lipid ◽  
The Third ◽  
Hepatic Lipid Peroxidation

This study was carried out to evaluate the possible mechanisms through which an aqueous extract from MO leaves demonstrates hepatoprotective effects in alloxan-induced diabetic rats. Eighty albino rats were assigned to 4 groups. The control group was orally administered sterile saline. The second group was injected with alloxan (150 mg/kg body mass (b.m.)) by intraperitoneal injection (i.p.). The third group was given MO (250 mg/kg b.m.) orally, daily. The fourth group was injected with alloxan, as for the second group, and administrated an aqueous extract of MO leaves, as for the third group. Alloxan induced degenerative changes in hepatic and pancreatic tissues, increased hepatic lipid peroxidation, and increased gene expression of PC and caspase 3. However, it decreased the activities of hepatic SOD and CAT, and gene expression of GS. In contrast, the MO extract prevented changes to the histoarchitecture of hepatic and pancreatic tissues and normalized the reduced hepatic levels of glutathione, as well as the activities of SOD and CAT, and the gene expression of GS, while reducing blood glucose levels, hepatic lipid peroxidation, and the gene expression of PC and caspase 3. This study indicated that an aqueous extract of MO leaves can be a potent antioxidant and used as an hepatoprotective agent.

scholarly journals Evaluation of Antidiabetic Activity of Polyherbal Formulation “Vasant Kusumakar Ras” on Alloxan-induced and Dexamethasone-induced Diabetic Rats

2021 ◽  
pp. 13-22
Author(s):  
Talha Jawaid ◽  
Kumari Nishu ◽  
Mehnaz Kamal ◽  
Saud M. Alsanad
Keyword(s):  
Blood Glucose ◽  
Group Treatment ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Antidiabetic Activity ◽  
Control Group ◽  
Albino Rats ◽  
Reference Drug ◽  
Polyherbal Formulation ◽  
Diabetic Control Group

Aim: The current study observed the antidiabetic effect of Vasant Kusumakar Ras, an Ayurvedic polyherbal formulation, in alloxan-induced and dexamethasone-induced diabetic rats. Materials and Methods: Alloxan (120 mg/kg, i.p.) and dexamethasone sodium phosphate (5 mg/kg, i.p.) were used to induce diabetes in rats. The oral antidiabetic activity of Vasant Kusumakar Ras was evaluated by single doses of Vasant Kusumakar Ras (400 and 600 mg/kg, p.o.) in albino rats during a 10-day treatment period, with the effect of the Vasant Kusumakar Ras on blood glucose levels and serum lipid parameters measured on 0, 7th, and 11th day. Glibenclamide (5 mg/kg, p.o.) was used as the reference drug. Results: In alloxan-induced diabetic rats, the elevated levels of blood glucose significantly (p < 0.05) decreased after oral administration of Vasant Kusumakar Ras (400 mg/kg and 600 mg/kg), and Glibenclamide (5 mg/kg). When compared to the diabetic control group, treatment with Vasant Kusumakar Ras and Glibenclamide for 10 days reduced total cholesterol (TC) significantly (p < 0.001). Treatment with Vasant Kusumakar Ras and Glibenclamide for 10 days, significantly (p < 0.001) decreased low-density lipoprotein (LDL) level when compared to the diabetic control group. In dexamethasone-induced diabetic rats, all rats given with dexamethasone and Vasant Kusumakar Ras (400 mg/kg and 600 mg/kg) showed a significant (p < 0.05) decrease in the level of blood glucose when compared with diabetic control rats. The rats treated with dexamethasone and Glibenclamide showed a significant (p < 0.05) decrease in blood glucose level when compared to diabetic control rats. When compared to the diabetic control group, treatment with Vasant Kusumakar Ras and Glibenclamide (5 mg/kg) for 10 days reduced TC significantly (p < 0.001). Treatment with Vasant Kusumakar Ras and Glibenclamide for 10 days, significantly (p < 0.001) decreased LDL level when compared to the diabetic control group. Conclusion: Vasant Kusumakar Ras was shown to have significant antidiabetic activity comparable to that of glibenclamide and it also improves the lipid metabolism in both alloxan-induced and dexamethasone-induced diabetic rats.

scholarly journals Anti-diabetic Potential of Aqueous Extract of Moringa oleifera, Ocimum gratissimum and Vernonia amygdalina in Alloxan-Induced Diabetic Rats

2020 ◽  
Author(s):  
A. L. Ayobami ◽  
E. A. Kade ◽  
K. A. Oladimeji ◽  
S. Kehinde ◽  
K. Gurpreet
Keyword(s):  
Body Weight ◽  
Aqueous Extract ◽  
Fasting Blood Glucose ◽  
Negative Control Group ◽  
Negative Control ◽  
Diabetic Rats ◽  
The Body ◽  
Control Group ◽  
Vernonia Amygdalina ◽  
Ocimum Gratissimum

AbstractThe incidence of diabetes mellitus (DM) is increasing globally and it is a major source of concern. This study was undertaken to assess the antidiabetic effect of the aqueous extract of Moringa oleifera, Ocimum gratissimum and Vernonia amygdalina. Sixty adult Wistar rats with body weight of 120-150 g were randomly assigned to groups of five rats each (n=12). Groups 1 served as normal control; Groups 2-5 were diabetic groups; group 2 served as negative control; group 3-5 received 100, 200 and 400 mg/kg of triherbal formula respectively. The body weight (BW) and fasting blood glucose level (FBSL) of the rats were monitored weekly. At the end of the experiment, all the rats were anaesthetized intraperitoneally (I.P) and blood samples were collected by cardiac puncture for biochemical analysis. There was an increase in the BW of the control group and varying doses of tri-herbal formation. It caused 88.0% decrease in FBSL; 371.7%, 386.6% and 296.0% with respect to 100, 200 and 400 mg/kg. Sub-chronic study of the effect of the extract showed a significant increase (P<0.05) in packed cell volume (PCV), white blood counts in rat induced diabetes. The histological studies showed that the diabetic rats with the architecture of the pancreas distorted, was restored to normal by the extract. Its LD50 was found to be greater than 1000 mg/kg indicating its safety in rats. This study has shown that triherbal formula has hypoglycemic and haematogical effects.

scholarly journals ANTIDIABETIC ACTIVITY OF SENNA SURATTENSIS IN ALLOXAN-INDUCED DIABETIC RATS

2018 ◽  
Vol 11 (4) ◽  
pp. 160 ◽  
Author(s):  
Ellappan Thilagam ◽  
Kumarappan Chidambaram ◽  
Subhash Chandra Mandal
Keyword(s):  
Total Protein ◽  
Density Lipoprotein ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Serum Biomarkers ◽  
Lipoprotein Cholesterol ◽  
Antidiabetic Activity ◽  
Control Group ◽  
Albino Rats ◽  
Present System

 Objective: Senna surattensis is a shrub plant which has been known for its diverse biological and pharmacological properties. This study is aimed to evaluate the antidiabetic activity of ethanolic extracts of S. surattensis (EESS) leaves in alloxan-induced diabetic rats.Methods: Experimental diabetes was induced by injection of a single dose of alloxan (120 mg/kg, intraperitoneal). Adult male Wistar albino rats were divided into five groups; normal control, diabetic control, diabetic EESS (200 mg/kg body weight (bw), diabetic EESS (400 mg/kg bw), and diabetic glibenclamide (5 mg/kg bw). Extracts were treated concurrently for 21 days. Blood samples were collected and centrifuged for estimation of fasting blood glucose (FBG), bw, serum biomarkers, lipid profile, total protein, albumin, and glycosylated hemoglobin (HbA1C) contents.Results: The increase in FBG, bw, liver biomarkers serum glutamic oxaloacetic transaminase, serum glutamic pyruvic transaminase, alkaline phosphatase, free fatty acid, phospholipids (PL), triglycerides, total cholesterol, low-density lipoprotein cholesterol, high-density lipoprotein cholesterol, total protein, albumin, and HbA1C content were recorded in diabetic control rats. Daily oral administration of EESS treatment significantly (p<0.01) reverted the levels of serum biomarkers and enzymes activities to near normal values. A similar reduction was produced in FBG after 21 days of extract administration which compared significantly (p<0.01) with the control group and glibenclamide treated groups.Conclusion: The results suggest that EESS has anti-diabetic activity in diabetic rats, thereby justifying its traditional claim and augmenting it into the present system of medicine.

scholarly journals Effects of Aqueous Extract of Vigna unguiculata Seedlings Against Doxorubicin-Induced Cardiotoxicity and Kidney damage In Female Albino Rats

2016 ◽  
Vol 1 (04) ◽  
Author(s):  
Olatunde A. Oseni ◽  
Ademola S. K. Idowu ◽  
Aminat Isah
Keyword(s):  
Body Weight ◽  
Aqueous Extract ◽  
Vigna Unguiculata ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Albino Rats ◽  
Group 2 ◽  
Wistar Albino Rats ◽  
Enzyme Markers

Doxorubicin is an anthracycline drug which is believed to cause immediate damage to myocardial cells by free radical generation in the cause of treatment of cancer. This study was however aimed to investigate the effects of the aqueous extract of the seedlings of Vigna unguiculata on heart, kidney as well as lipid profile disorders caused by this drug on female Wistar albino rats. Fifteen female Wistar albino rats were divided into three groups. Group 1 animals served as normal positive control; Group 2 animals served as negative control which were treated with 0.5mL of (20mg/kg body weight doxorubicin) while Group 3 animals were treated with 0.5mL each of 20mg/kg body weight doxorubicin and 10% aqueous extract of seedlings of Vigna unguiculata. Some enzyme markers and lipid contents were determined. The results of the study showed an increase in the activities of plasma ALP and AST after treatment with the drug except in the heart for AST which showed a significant reduction, while treatment with the extract brought about a decrease in the plasma and the organs except for kidney AST. The ALT on the other hand showed slight increase in the plasma with a decrease in the kidney and heart after treatment with the drug as the treatment with the extract tend to restore it to the control in both cases, there was increased plasma and kidney but reduced heart HDL-C after treatment with the drug which was observed to be restored to control after treatment with extract in both situations. The triglyceride and total cholesterol did not show similar trend in the plasma and the studied organs. The LDL-C was also observed to be increased in both plasma and organs after the treatment with the drug which later reduced significantly towards the control after the administration of the sprout extract. The present study has shown that the doxorubicin has damaging effects on both kidney and heart tissues while the sprouts extract produce a restoration to the normal in both organs.

scholarly journals Antidiabetic Activity of Extracts of Pithecellobium dulce Benth Leaves in Alloxan Induced Diabetic Rats

2016 ◽  
Vol 8 (05) ◽  
Author(s):  
V. S. Mul ◽  
N. Naikwade ◽  
C. Magdum ◽  
V. Jagtap
Keyword(s):  
Uric Acid ◽  
Blood Glucose ◽  
Total Cholesterol ◽  
Ethanolic Extract ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Antidiabetic Activity ◽  
Control Group ◽  
Albino Rats ◽  
Glycogen Level

The objective of the present study was to study effect of Pithecellobium dulce Benth (P. dulce) leaves in alloxan induced diabetic rats. The P. dulce leaves were extracted by maceration and soxhelation method by using water and ethanol as solvent. Acute toxicity study was performed according to OECD 425 guidelines for both aqueous and ethanolic extracts of P. dulce leaves. The dose of 200 mg/kg and 400 mg/kg was selected for further studies. Animals were rendered diabetic by administration of alloxan (130 mg/kg, i.p.). The albino rats were divided in to seven groups with five animals in each group. Diabetic animals were treated with aqueous and ethanolic extract for 20 days. Then blood glucose, triglyceride, total cholesterol, urea, uric acid, creatinine, aspartate aminotransferase (AST), alanine transaminase (ALT) and glycogen level in liver, muscle and kidney were estimated according to standard procedures. The result shows significant decrease in blood glucose, triglyceride, total cholesterol, urea, uric acid, creatinine, AST and ALT level when compared to diabetic group. The liver and muscle glycogen level was increased significantly in extract treated groups when compared to diabetic control group. Both extract of P. dulce posses antidiabetic and hypolipidemic potential.

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