scholarly journals In vitro fermentation evaluation of selected agro-industrial by-products as alternative feed for ruminants

2020 ◽  
Vol 47 (4) ◽  
pp. 219-226
Author(s):  
D. O. Ogunbosoye ◽  
K. B. Salau
Keyword(s):  
Rice Bran ◽  
Short Chain Fatty Acids ◽  
Metabolizable Energy ◽  
In Vitro Fermentation ◽  
Feed Resources ◽  
Alternative Feed ◽  
Corn Bran ◽  
Gas Fermentation ◽  
Gas Volume

In the tropic there is seasonal variation in the quality and quantity of forage which affects the sustainability of all year round ruminant production. There is need to source for cheaper and readily available feed to supply the nutrients required by the animals. Hence, this study was conducted to investigate the in vitro gas fermentation and its characteristics on selected agro-industrial -products (AIBs) as alternative feed resources for ruminant animals. Corn bran (CB), soybean cheese waste (SBCW), shea nut cake (SNC) and Rice bran (RB) were incubated for 24hrs. The cumulative gas volume at the end of incubation was measured. Methane (ml/200mgDM) production of the AIBs was measured at the termination of the incubation with the introduction of 10ml NaOH into the syringes. The in-vitro fermentation characteristic, metabolizable energy (ME), organic matter digestibility (OMD) and short chain fatty acids (SCFA) were estimated. Proximate composition of the AIBs showed that crude protein (CP) ranged from 13.80% in RB to 30.12% in SBCW. Rice bran also had the highest ash content (17.20%) while the SNC recorded highest concentration of crude fiber. The SNC and SBCW had similar ether extract (EE) content. Gas volume statistically varied from 12.00ml/200mgDM to 27.67ml/200mgDM in rice bran and corn bran, respectively. The CB produced highest values of the fermentation characteristics. Methane production ranged from 2.5 to 5.5mLs in rice bran and corn bran, respectively. The calculated values of ME, SCFA and OMD were highest in SBCW. Overall, higher nutritive values found in the individual AIBs evaluated suggest that they are good alternative feed resources and when combined in the right proportion would sustain and improve ruminant productivity during the dry season.

scholarly journals Dietary Fiber Modulates the Fermentation Patterns of Cyanidin-3-O-Glucoside in a Fiber-Type Dependent Manner

Foods ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1386
Author(s):  
Zixin Yang ◽  
Ting Huang ◽  
Ping Li ◽  
Jian Ai ◽  
Jiaxin Liu ◽  
...  
Keyword(s):  
Dietary Fiber ◽  
Fiber Type ◽  
Short Chain Fatty Acids ◽  
Dependent Manner ◽  
Dietary Fibers ◽  
Cell Wall Polysaccharides ◽  
Total Carbohydrate ◽  
In Vitro Fermentation ◽  
Gas Volume

The interactions between cell-wall polysaccharides and polyphenols in the gastrointestinal tract have attracted extensive attention. We hypothesized that dietary fiber modulates the fermentation patterns of cyanidin-3-O-glucoside (C3G) in a fiber-type-dependent manner. In the present study, the effects of four dietary fibers (fructose-oligosaccharides, pectin, β-glucan and arabinoxylan) on the modulation of C3G fermentation patterns were investigated through in vitro fermentation inoculated with human feces. The changes in gas volume, pH, total carbohydrate content, metabolites of C3G, antioxidant activity, and microbial community distribution during in vitro fermentation were analyzed. After 24 h of fermentation, the gas volume and total carbohydrate contents of the four dietary-fiber-supplemented groups respectively increased and decreased to varying degrees. The results showed that the C3G metabolites after in vitro fermentation mainly included cyanidin, protocatechuic acid, 2,4,6-trihydroxybenzoic acid, and 2,4,6-trihydroxybenzaldehyde. Supplementation of dietary fibers changed the proportions of C3G metabolites depending on the structures. Dietary fibers increased the production of short-chain fatty acids and the relative abundance of gut microbiota Bifidobacterium and Lactobacillus, thus potentially maintaining colonic health to a certain extent. In conclusion, the used dietary fibers modulate the fermentation patterns of C3G in a fiber-type-dependent manner.

scholarly journals Chemical composition and in vitro digestibility of some selected under-utilized tropical seeds as protein sources in ruminants’ diet

2020 ◽  
Vol 44 (1) ◽  
Author(s):  
Oluwatosin Bode Omotoso ◽  
Mary Oluwafunmilayo Adeduntan ◽  
Adebowale Noah Fajemisin
Keyword(s):  
Short Chain Fatty Acids ◽  
Nutritional Composition ◽  
Metabolizable Energy ◽  
In Vitro Digestibility ◽  
Protein Sources ◽  
Dry Matter Digestibility ◽  
Supplementary Feed ◽  
Monodora Myristica

Abstract Background The study highlighted the potential of three common and under-utilized tropical leguminous seeds (Tomentosa nilotica, Dioclea reflexa and Monodora myristica) to be used as supplementary feed to ruminant livestock. These seeds (their plants inclusive) are valuable sources of food and medicine for the prevention of illness and maintenance of human health. The medicinal properties of these seeds include antimicrobial, anti-inflammatory, anti-oxidant and immuno-stimulant. Trypsin inhibitors, which are common anti-nutritional factors in legumes and for monogastric animals, do not exert adverse effects in ruminants because they are degraded in the rumen. Hence, the crux of this study is to examine the effect of processing methods on the nutritional composition (proximate, fibre fractions, minerals, anti-nutrients) and in vitro digestibility of Tomentosa nilotica, Dioclea reflexa and Monodora myristica seeds and their suitability as feedstuff (protein sources) in small ruminant feed, particularly during off-season. Results From the results, raw Tomentosa nilotica and Monodora myristica have the highest crude protein (30.35% CP) and fat (22.40% EE), respectively. It is noteworthy that roasting best improve the mineral and significantly reduce the anti-nutrients observed in this study better compared to boiling and soaking methods. The highest organic matter digestibility, short-chain fatty acids, metabolizable energy and in vitro dry matter digestibility values were obtained in Dioclea reflexa compared to other test seeds. Roasting best improved the nutritive values, while Dioclea reflexa seed was rated highest for all the nutritional attributes and in vitro digestibility. Conclusions Dioclea reflexa could be incorporated in ruminants’ diet as protein source, particularly during the off-season, for improved ruminant production in Nigeria. However, in vivo study is therefore recommended to validate this report.

scholarly journals In vitro fermentation of rice bran combined with Lactobacillus acidophilus 14 150B or Bifidobacterium longum 05 by the canine faecal microbiota

2011 ◽  
Vol 75 (3) ◽  
pp. 365-376 ◽  
Author(s):  
Eva Ogué-Bon ◽  
Christina Khoo ◽  
Lesley Hoyles ◽  
Anne L. McCartney ◽  
Glenn R. Gibson ◽  
...  
Keyword(s):  
Rice Bran ◽  
Lactobacillus Acidophilus ◽  
Bifidobacterium Longum ◽  
Faecal Microbiota ◽  
In Vitro Fermentation

scholarly journals In Vitro Fecal Fermentation Patterns of Arabinoxylan from Rice Bran on Gut Microbiota in Normal Weight and Overweight/Obese Subjects

2020 ◽  
Vol 4 (Supplement_2) ◽  
pp. 1560-1560
Author(s):  
Inah Gu ◽  
Wing Shun Lam ◽  
Daya Marasini ◽  
Cindi Brownmiller ◽  
Brett Savary ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Rice Bran ◽  
Block Design ◽  
16S Rrna Gene Sequencing ◽  
Short Chain Fatty Acids ◽  
Normal Weight ◽  
Rrna Gene ◽  
Gut Health ◽  
The Impact

Abstract Objectives Arabinoxylan is a non-starch polysaccharide and rich in wheat, rice and many other cereal grains. Diets high in fiber help promoting gut health in obesity. The objective of this study was to investigate the impact of arabinoxylan from rice bran on the gut microbiota and short chain fatty acids (SCFA) in normal weight (NW) and overweight/obese (OO) subjects through in vitro fecal fermentation. Methods Arabinoxylan was extracted from rice bran fiber. For in vitro fecal fermentation, each fecal sample from NW (n = 6, 3 males and 3 females) and OO (n = 7, 3 males and 4 females) was diluted into anaerobic medium with three treatments: control (no substrates), fructooligosaccharides (FOS, a well-known prebiotic), and arabinoxylan. Samples were incubated at 37˚C and aliquots were taken at 0, 4, 8, 12 and 24 h. SCFA content from samples at all timepoints was analyzed using HPLC. Samples at 0 and 24 h were used for gut microbiota analysis through 16S rRNA gene sequencing. Statistical analyses were performed for the randomized complete block design, where the weight classes are confounded with blocks (subjects). Friedman test was used to determine the difference at 5% level of significance. Results As a result, arabinoxylan treatment significantly increased total SCFA concentration in both NW and OO subjects than control (P < 0.05), comparable to FOS treatment. Between weight classes under arabinoxylan treatment, OO group showed a significantly higher total SCFA content than NW group (P < 0.05). Arabinoxylan changed gut microbial population at the genus level, stimulating Bifidobacterium, Collinsella and Blautia and decreasing Clostridium XIVa and b, Dorea and Oscillibacter (P < 0.05). In addition, different microbiome population was shown in weight classes with three treatments, showing higher Bacteroides in NW and higher Prevotella in OO. Conclusions These results showed that arabinoxylan from rice bran modified gut microbiota in both weight classes, increasing total SCFA content. This study suggests that arabinoxylan from rice bran may have a potential impact on microbial gut health in obesity with prebiotic activities. Funding Sources University of Arkansas.

scholarly journals Short Chain Fatty Acid Production from Mycoprotein and Mycoprotein Fibre in an In Vitro Fermentation Model

Nutrients ◽  
2019 ◽  
Vol 11 (4) ◽  
pp. 800 ◽  
Author(s):  
Hannah Harris ◽  
Christine Edwards ◽  
Douglas Morrison
Keyword(s):  
Energy Intake ◽  
Short Chain Fatty Acid ◽  
Short Chain Fatty Acids ◽  
Chain Fatty Acid ◽  
Fibre Content ◽  
Short Chain ◽  
Fatty Acid Production ◽  
In Vitro Fermentation ◽  
Fermentation Model

Dietary mycoprotein (marketed as QuornTM) has many health benefits, including reductions in energy intake. The majority of studies evaluating mycoprotein focus on the protein content and very few consider the fibre content. Fibre consumption is also associated with decreased energy intake, which is partly attributed to short chain fatty acids (SCFAs) from fibre fermentation by colonic bacteria. To study the SCFA-producing capability of mycoprotein, in vitro batch fermentations were conducted, and SCFA production compared with that from extracted mycoprotein fibre, oligofructose (OF), rhamnose, and laminarin. Mycoprotein and mycoprotein fibre were both fermentable, resulting in a total SCFA production of 24.9 (1.7) and 61.2 (15.7) mmol/L, respectively. OF led to a significantly higher proportion of acetate compared to all other substrates tested (92.6 (2.8)%, p < 0.01). Rhamnose generated the highest proportion of propionate (45.3 (2.0)%, p < 0.01), although mycoprotein and mycoprotein fibre yielded a higher proportion of propionate compared with OF and laminarin. Butyrate proportion was the highest with laminarin (28.0 (10.0)although mycoprotein fibre led to a significantly higher proportion than OF (p < 0.01). Mycoprotein is a valuable source of dietary protein, but its fibre content is also of interest. Further evaluation of the potential roles of the fibre content of mycoprotein is required.

scholarly journals In Vitro Fermentation of Sheep and Cow Milk Using Infant Fecal Bacteria

Nutrients ◽  
2020 ◽  
Vol 12 (6) ◽  
pp. 1802 ◽  
Author(s):  
Natalie Ahlborn ◽  
Wayne Young ◽  
Jane Mullaney ◽  
Linda M. Samuelsson
Keyword(s):  
Fatty Acids ◽  
Gut Microbiota ◽  
Large Bowel ◽  
Short Chain Fatty Acids ◽  
Fecal Microbiota ◽  
Cow Milk ◽  
Fecal Material ◽  
In Vitro Fermentation ◽  
Sheep Milk

While human milk is the optimal food for infants, formulas that contain ruminant milk can have an important role where breastfeeding is not possible. In this regard, cow milk is most commonly used. However, recent years have brought interest in other ruminant milk. While many similarities exist between ruminant milk, there are likely enough compositional differences to promote different effects in the infant. This may include effects on different bacteria in the large bowel, leading to different metabolites in the gut. In this study sheep and cow milk were digested using an in vitro infant digestive model, followed by fecal fermentation using cultures inoculated with fecal material from two infants of one month and five months of age. The effects of the cow and sheep milk on the fecal microbiota, short-chain fatty acids (SCFA), and other metabolites were investigated. Significant differences in microbial, SCFA, and metabolite composition were observed between fermentation of sheep and cow milk using fecal inoculum from a one-month-old infant, but comparatively minimal differences using fecal inoculum from a five-month-old infant. These results show that sheep milk and cow milk can have differential effects on the gut microbiota, while demonstrating the individuality of the gut microbiome.

scholarly journals In Vitro Fermentation Patterns of Rice Bran Components by Human Gut Microbiota

Nutrients ◽  
2017 ◽  
Vol 9 (11) ◽  
pp. 1237 ◽  
Author(s):  
Tung Pham ◽  
Keat Teoh ◽  
Brett Savary ◽  
Ming-Hsuan Chen ◽  
Anna McClung ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Rice Bran ◽  
Human Gut ◽  
In Vitro Fermentation ◽  
Human Gut Microbiota

scholarly journals Nutritional contents of processed Delonix regia seeds incubated in vitro with Pennisetum purpureum

2020 ◽  
Vol 45 (1) ◽  
pp. 309-316
Author(s):  
O. O. Olufayo ◽  
O. O. Falola
Keyword(s):  
Dry Matter ◽  
Nutritive Value ◽  
Short Chain Fatty Acids ◽  
Gas Production ◽  
Metabolizable Energy ◽  
Pennisetum Purpureum ◽  
Crude Fibre ◽  
Delonix Regia ◽  
Dry Matter Digestibility

The effect of processing on proximate, mineral, anti-nutritional compositions, gasproduction characteristics, methane production, predicted metabolizable energy, organic matter digestibility and short chain fatty acids production of Delonix regia seeds incubated with Pennisetum purpureum were determined. Five treatments (T1: mixture of soaked Delonix regia seed and Pennisetum purpureum; T2: mixture of raw Delonix regia seed and Pennisetum purpureum; T3: mixture of roasted Delonix regia seed and Pennisetum purpureum; T4: mixture of boiled Delonix regia seed and Pennisetum purpureum and T5:100% Pennisetum purpureum which serves as control) were designed. In vitro gas production techniques for 24 hours were used to determine the nutritive value of processed 50% Delonix regia seed and 50% Pennisetum purpureum. The crude protein ranged from 13.38 to 15.71g/100g DM among the treatments while crude fibre was between 20.10 and 21.40g/100g DM, ether extract ranged from 1.90 to 3.12g/100g DM and ash 7.77 to 12.34g/100g DM. Calcium ranged from (2.34 - 3.61%), magnesium ranged (2.90 – 3.48%), sodium (2.05 – 2.91%) and phosphorus (0.88 – 1.12%). It was observed generally that T2 recorded the highest among the treatments while the values obtained for the heat treated seeds were lower than the raw. Oxalates ranged between (0.23% - 0.32%), phytates (0.29 – 0.43%), tannin (0.03 – 0.08%) and saponnin (0.36 – 0.49%). Methane (ml/200mg DM) production indicated T3 (50% roasted Delonix regia seed and 50% Pennisetum purpureum) was highest. The potential gas production 'a+b' ranged from 12.33 to 28.33mL/200mg DM). The highest potential gas production 'a+b' value of 28.33mL/200mg DM was obtained in T1 compared to other dietary treatment. Dry matter digestibility (DMD) ranged between 70.23 – 91.02% while the rate of fermentation was between 0.51 and 1.18ml/hr. The rate of fermentation was directly proportional to dry matter digestibility (DMD); the soaked recorded the highest value. Processing enhanced the nutritional contents of Delonix regia seeds. The result obtained showed that there were significant differences (p<0.05) among the treatments. In conclusion, soaking the seeds of Delonix regia had beter nutritional contents and has the potential for dry season feeding.  

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