Features of ultrafiltration of milk obtained from cows with CSN3 polymorphism

2021 ◽  
pp. 33-35
Author(s):  
Александр Геннадьевич Кручинин ◽  
Елена Евгеньевна Илларионова ◽  
Светлана Николаевна Туровская ◽  
Алана Владиславовна Бигаева

Мембранные процессы широко внедрены на современных предприятиях молочной промышленности, поскольку они способствуют обеспечению максимальной степени использования сырья и повышению качества готовой продукции. При этом они без затруднений интегрируются в классическую технологическую цепочку. Научные изыскания, направленные на дальнейшее совершенствование технологий баромембранного концентрирования молочного сырья, а также использование генетического подхода к формированию его технологических свойств весьма актуальны. Целью данной работы являлось исследование закономерностей процесса ультрафильтрации обезжиренного молока, полученного из молока коров с генотипами АА и ВВ по гену CSN3. Объектом исследований служило обезжиренное молоко, полученное из молока от КРС черно-пестрой породы с гомозиготными генотипами по гену CSN3 (АА и ВВ). Концентрирование молока осуществляли на пилотной установке с полиэфирсульфоновыми мембранами с порогом задержки по молекулярной массе 50 кДа при температуре 15…20 °С и объемном факторе концентрирования 3,5. В результате исследований выявлена закономерность снижения скорости процесса ультрафильтрации вследствие большей эффективности концентрирования массовых долей белка и сухих веществ обезжиренного молока, полученного из молока коров с генотипом АА по гену CSN3, по отношению к молоку от коров с генотипом ВВ. Membrane processes are widely used in modern dairy production, as they help to maximize the use of raw materials and improve the quality of end product. At the same time, they are easily integrated into the classical technological chain. Further improvement of the baromembrane concentration of dairy raw products technologies and the use of the genetic approach to the formation of its technological properties is an urgent scientific study today. The aim of this work was to study the regularities of the ultrafiltration process of skim milk obtained from the milk of cows with genotypes AA and BB according to the CSN3 gene. The object of research was skim milk obtained from milk from black-and-white cattle with homozygous genotypes for the CSN3 gene (AA and BB). Milk concentration was carried out on a pilot plant with polyethersulfone membranes with a retention threshold in molecular weight of 50 kDa at a temperature of 15…20 °C and a volumetric concentration factor of 3.5. As a result of the research, a regularity of the decrease in the rate of the ultrafiltration process was revealed due to the greater efficiency of concentration of mass fractions of protein and dry matter of skim milk obtained from milk of cows with the AA genotype according to the CSN3 gene in relation to milk from cows with the BB genotype.

2019 ◽  
Vol 13 (2) ◽  
Author(s):  
G. Deinychenko ◽  
Т. Lystopad ◽  
T. Kolisnychenko

The current thing now is the matter regarding enrichment of food products with organic iodine, which is caused by iodine deficiency in the food ration of most of the country's population and by the country-wide radionuclide pollution, which leads to a hundredfold increase of risks of the thyroid body radiation. In this regard, there were developed the technologies of the berry sauces with the addition of the seaweed’s raw materials, as the iodine-concentrating component. The technologies of blueberry-cranberry sauces with guelder-rose juice, cornel-bilberry sauce with guelder-rose juice and blueberry-buckthorn sauce with guelder-rose juice were developed. This work is dedicated to a research of safety indicators of the developed sauces. There were used the hydrated seaweeds of Laminaria, of Fucus, of Undaria pinnatifida as iodinated additives. The following microbiological indicators were determined: the quality of mesophilic aerobic and optionally anaerobic microorganisms (МAOАnM), mold, yeast and lactic acid bacteria, and the presence of the coliform bacteria (CB) bacteria. The study results showed, that there are no microorganisms in the finished products, which may result the damage of the product after pasteurization. It was identified, that the own organic acids of the prepared sauces will be sufficient to ensure the microbiological clearness and the use of the additional preserving agents does not make sense. Toxicological indicators, namely the mass fractions of lead, cadmium, arsenic and mercury, have been studied. It has been established that the content of toxic elements in test samples is hundreds of times less than permissible. The toxicological indicators were studied, namely, the mass fractions of lead, cadmium, arsenic and mercury. It was identified, that the content of toxic elements in test samples is hundreds of times less than permissible. Thus, it is proved that the sauces made according to the developed technology, comply with the requirements of the regulatory documents according to the safety indicators. Moreover, it is proved, that that the addition of the seaweed’s raw materials does not worsen these indicators. To confirm the obtained data, the sample with the highest seaweed content was examined by an independent laboratory accredited according to DSTU ISO/IEC 17025 with the issuance of the appropriate test report.


2003 ◽  
Vol 46 (5) ◽  
pp. 405-411 ◽  
Author(s):  
R. Skrzypek ◽  
J. Wójtowski ◽  
R.-D. Fahr

Abstract. The investigations were carried out in the year 2001 on 167 Black-and-White cattle farms of Wielkopolska region (West Poland). On the farms included in the study a survey on performing the premilking procedures was conducted and the results were collected concerning analyses of bulk tank milk samples for the somatic cell count (SCC) and total microorganism count (TMC). Herds, in which udders and teats were cleaned with a towel soaked with a disinfectant or washed with water containing a disinfectant, were characterized by lowest logeSCC (12.36 and 12.41, respectively). They were followed by herds in which wiping with a dry towel (12.48) and washing with clean water (12.58) were practiced. The lowest logeTMC was observed in the herds in which udders and teats were cleaned with a dry towel (10.66). This characteristic was similar in the herds where washing with clean water or wiping with a towel soaked with a disinfectant were practiced (10.89 and 11.00, respectively). The highest value of logeTMC was in the herds in which washing with water containing a disinfectant was practiced (11.28). For SCC, a significant interaction was found between both pre-milking procedures. In the case of wiping with either a dry or wet towel, significantly lower values of logeSCC were observed in the herds in which forestripping was performed prior to cleaning. However, in the herds in which udders and teats were washed with water, a better option was to perform forestripping after udder and teat cleaning.


Author(s):  
I. A. Ilina ◽  
I. A. Machneva ◽  
E. S. Bakun

  The article is devoted to the study of the chemical composition, physical and thermal-pfysical characteristics of damp apple pomaces and the identifying patterns of influence of drying temperature the functional composition and gel-forming ability of pectin. The research is aimed at obtaining initial data for the subsequent calculation of the main technological, hydro-mechanical, thermal, structural and economic characteristics of devices for drying the plant raw materials, ensuring the environmental safety and high quality of pectin-containing raw materials, the reducing heat and energy costs. As a result of the study of the thermal characteristics of apple pomaces, the critical points (temperature conductivity – 16.5 x 10-8 m2/s, thermal conductivity – 0.28 W/m K, heat capacity – 1627 j/(kg K)) at a humidity of 56 % are determined, which characterizing the transition from the extraction of weakly bound moisture to the extraction of moisture with strong bonds (colloidal, adsorption). It was found that the pomaces obtained from apples of late ripening have a higher content of solids (21-23 %), soluble pectin and protopectin (2.5-4.5 %). Dried pomaces obtained from apple varieties of late ripening contain up to 25 % pectin, which allow us to recommend them as a source of raw materials for the production of pectin. The optimum modes of preliminary washing of raw materials are offered, allowing to the remove the ballast substances as much as possible. It is established that when the drying temperature increases, the destructive processes are catalyzed: the strength of the pectin jelly and the uronide component and the degree of pectin esterification are reduced. The optimum drying temperature of damp apple pomaces is 80 0C, at which the quality of pectin extracted from the dried raw materials is maintained as much as possible. It is shown that the most effective for the pectin production is a fraction with a particle size of 3-5 mm, which allow us to extract up to 71 % of pectin from raw materials.


2019 ◽  
Author(s):  
Onsardi Onsardi

The title of this study is the Strategy of Increasing Consumer Food Loyalty in CurupCity, Rejang Lebong Regency (Case Study in "Henvian" Typical Food Industry). Thisresearch is based on the importance of strategies in increasing business and consumerloyalty to products sold.Strategies to increase business and consumer loyalty can bedone with a SWOT analysis. Place of this research is the "Henvian" shop that sellstypical Rejang lebong food. The method used in this study is descriptive qualitative.Informants in this study were people who were considered to know for certain about theHENVIAN Specialty Food Store in Curup City, Rejang Lebong Regency. The dataanalysis technique used in this study is a SWOT analysis to determine the strengths,weaknesses, opportunities and threats in a typical Rejang Lebong food business.By using SWOT analysis techniques that consist of strengths (weakness), weaknesses(weakness), opportunities (opportnity) and threats (threath). The results of this studycan be concluded that the internal factors that are the strength of the marketing strategyare the quality of the product that is good at a price affordable to the public andtourists, service that is friendly and responsive to consumer needs, as well astechnological advancements that facilitate the promotion of business. Internal factorsthat are a weakness are often lack of stock, there are some products that do not meet thestandard packaging, the product shelf life is short, employees do not use uniforms.External factors that become opportunities are a fairly high economic community,abundant raw materials while external factors that are a threat are the manycompetitors, an unstable economy, the price of basic needs increases. Based on theresults of the SWOT analysis of internal and external factors, the strategy used is toimprove product quality by improving the appearance of packaging and quality ofcontent and quality of service by providing uniforms to employees and providingstandards of service to consumers. .Keywords: Strategy, Consumer Loyalty, SWOT


2020 ◽  
Vol 11 (2) ◽  
pp. 73-82
Author(s):  
А. Trubnikova ◽  
О. Chabanova ◽  
S. Bondar ◽  
Т. Sharakhmatova ◽  
Т. Nedobijchuk

Optimization of the formulation of synbiotic yogurt ice cream low-lactose using lactose-free protein concentrate of buttermilk and yogurt with low lactose content is the goal of expanding the range of low-lactose dairy products and improving the functional and health properties of ice cream. Low-lactose ice cream formulation optimization was performed using a gradient numerical method, namely conjugated gradients (Conjugate Gradient). The optimization algorithm is implemented in Mathcad. An array of data with a set of indicators for the choice of a rational ratio of lactose-free protein concentrate of buttermilk and yogurt base and inulin content for ice cream mixtures is presented. The influence of the ratio of the main components of the mixtures on the foaming ability, which determines the quality of the finished product, has been studied. An important indicator is taken into account - the concentration factor of buttermilk, which is additionally purified from lactose by diafiltration. The graphic material presented in the work clearly demonstrates that the rational ratio of yogurt base and lactose-free protein concentrate of buttermilk, obtained by ultrafiltration with diafiltration purification at a concentration factor of FC = 5 is 40.6: 59.4. The content of additional components included in the recipe of a new type of ice cream is optimized in the work, the mass fractions of which were: inulin - 3.69 %; lactulose – 1 %; ginger - 0.3 %; citric acid - 0.15 %; stabilization system - 0.2 %. The chemical composition and quality indicators of the mixture for ice cream low-lactose synbiotic yogurt, consisting of raw materials in the optimal ratio, were determined. The lactose content in the test sample of the ice cream mixture was 0.99%, the antioxidant activity was 3.1 times higher than in the mixture for traditional yogurt ice cream. The most likely number of lactic acid microorganisms, CFU / cm3 is (2.8 ± 0.9) · 108, the number of bifidobacteria, CFU / cm3 is (2.5 ± 0.2) · 109. The results of the research will be implemented in dairy companies in the production of ice cream.


Author(s):  
L. Ovchinnikova ◽  
E. Babich

In the Republic of Kazakhstan over the past decade positive changes have been observed in increasing the number of farm animals, increasing their productivity, and increasing the gross production of milk, meat and other products. At the same time the development of dairy cattle breeding is mainly due to the breeding of Black-and-White, Red Steppe, Simmental breeds and their intra-breed types as well as imported from abroad Holstein breed. The purpose of the research was to analyze ways to improve the intra-breed type Karatomar of Black-and-White breed in Northern Kazakhstan. Research work has been carried out on breeding animals such as Karatomar type cattle of Black-and-White breed in LLP “Experimental farm Zarechnoe” in the Kostanay district in the Kostanay region. The dynamics of growth and development indicators, reproductive traits of heifers of a new intra-breed type Karatomar of Black-and-White cattle have been studied. A comprehensive assessment of their economically useful traits in the first lactation, determined by the resistance of animals to climatic and forage conditions, the efficiency of breeding of animals of different genotypes has been conducted. It has been found that the replacement heifers – daughters of American breeding sires inherited precocity and showed higher growth energy after 6 months of age and continued to grow rapidly until 18 months. The advantage in metabolism was in the daughters of Holstein sires of the American selection Orbit 4078, and the increased immune status of the organism in the daughters of the sire of the domestic selection Yamal 975 as the most adapted to the conditions of Northern Kazakhstan. The best ability to increasing the milk yield, stable lactation activity, high milk productivity, gross content of dry matter and nonfat milk solids have been characterized by the daughters of sires of the American selection Orbit 4078 and Lowrider 4129. Analysis of economic indicators of milk production has been shown that from cows – daughters of sires Orbit 4078 and Lowrider 4129 for 305 days of lactation received 15,8 and 15,2 % more milk of base fat, the expenditures of EFU per 1 kg of production was lower by 10,2 and 5,9 %. A higher level of profitability has been observed in the group of daughters of the sires Orbit 4078 – 35,5 % and Lowrider 4129 – 33,1 %, which was 8,5 and 10,8 % higher than in the group of daughters of the sire Yamal 975.


Author(s):  
S. Sukhanova ◽  
N. Pozdnyakova ◽  
F. Yaroslavtsev

The results of researches on the development of new mineral feed additives based on raw materials produced by Uralchem and the study of the eff ectiveness of their use in the rations of lactating cows have been presented in the article. The experiment has been carried out in the conditions of LLC “PF Barabinskoe” in the Dalmatovsky area in the Kurgan region where 3 groups of lactating cows of Black-and-White breed have been formed. Animals in the control group have been received the ration adopted in the farm, the 1st experimental group with the additive RusMA No. 1 at a dose of 150 g, the 2nd experimental group– with the additive RusMA No. 2 at the dose of 350 g. The use of experimental mineral additives in feeding lactating cows allowed us to increase their milk productivity and profi tability of production. At the same time, the best eff ectiveness was shown by the additive RusMA No. 2 at the dose of 350 g/head/day. The use of RusMA additives during the experiment period (105 days) allowed to increase the milk productivity of cows in the 1st experimental group by 6,03 % and in the 2nd experimental group by 8,24 %. Taking into account the fat content in milk, this diff erence was 6,31 and 8,53 %, respectively. The energy value of milk from cows of the experimental groups was higher compared to the control group by 2,45 and 1,76 %, respectively. According to the content of dry matter in milk, cows of the 2nd experimental group exceeded the control and 1st experimental group by 0,39 and 0,12 abs.%, respectively. The maximum milk fat content has been observed in the milk of cows of the 1st experimental group by 0,03 and 0,01 abs.% more than in the control and 2nd experimental groups, respectively. In terms of protein content, the cows of the 2nd experimental group signifi cantly exceeded the control group by 0,10 abs.% and the 1st experimental by 0,04 abs.%. The content of milk sugar in the 2nd experimental group was higher than in the control by 0,30 abs.% and compared to the 1st experimental by 0,11 %. Signifi cantly more calcium and phosphorus were contained in the milk of cows of the 2nd experimental group by 4,58 % (Р ≤ 0,001) and 2,86 % (Р ≤ 0,05), compared with the control group. The expenditures of feed in the EFU for the production of 1 kg of milk in animals of the 2nd experimental group was less by 5,13 % than in control cows and by 2,63 % compared to the 1st experimental group. The prime cost of 1 centner of milk was lower in the 2nd experimental group by 3,94 %, in the 1st experimental group by 2,65 % compared to the control group. The level of profi tability of milk production in the 2nd experimental group was higher by 9,0 and 1,0 abs.% in comparison with the control and 1st experimental groups, respectively.


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