scholarly journals COMPARAÇÃO ENTRE OS MEIOS DE CULTURA PARA CONTAGEM DE FUNGOS NO CONTROLE MICROBIOLÓGICO DE ERVA-MATE

Author(s):  
KATIA ELISA SAATKAMP LAZARETTI ◽  
MARCIA REGINA BEUX ◽  
IDA CHAPAVAL PIMENTEL ◽  
ANELISE TALAMINI ◽  
JUAREZ GABARDO

A contagem de bolores e leveduras é uma das análises realizadas no controle de qualidade de alimentos com intuito de estimar a vida útil de determinado produto alimentício. Embora considerada indicadora de contaminação, quantificar estes fungos é fundamental na avaliação da qualidade de produtos armazenados, principalmente cereais e preparados para infusões, em virtude do potencial micotoxigênico de algumas espécies de bolores. O presente trabalho teve por objetivo comparar o número de unidades formadoras de colônias (UFC) de fungos, obtidas a partir de três marcas de ervamate, semeadas em quintuplicata em ágar batata dextrosado, ágar dicloran rosa de bengala cloranfenicol e meio para bolores e leveduras ­ SIMPLATE. O ágar batata dextrosado e o ágar dicloran rosa de bengala cloranfenicol são rotineiramente empregados em laboratórios de análise, necessitando após inoculação, de cinco dias para leitura dos resultados. O meio para bolores e leveduras ­SIMPLATE é um método novo, baseado na atividade metabólica, que permite efetuar a leitura em dois dias após a inoculação, reduzindo significativamente o prazo analítico. Os resultados obtidos foram avaliados estatisticamente por meio da análise de variância e Teste de Tukey. Na prática laboratorial o melhor método é aquele que recupera maior número de unidades formadoras de colônias (UFC). Na amostra que apresentou contagem de até 10.000 UFC/g não se constatou diferença significativa entre os meios de cultura testados, porém nas amostras com contagens superiores a 10.000 UFC/g o ágar dicloran rosa de bengala cloranfenicol e o meio para bolores e leveduras ­SIMPLATE revelaramse estatisticamente mais eficazes que o ágar batata dextrosado. Abstract The mould and yeasts counting is one of the analyses accomplished in the control of quality of foods with an aim to estimate the useful life of certain nutritious product. Although considered indicative of contamination, to quantify these fungi is fundamental in the evaluation of the quality of stored products, mainly cereals and prepared for infusions, by virtue of the toxicant potential of some mould species. The present work had for objective to compare the number of forming colonies units (FCU) of fungi, obtained from three “mate” marks, sowed five fold in potato dextrose agar, dichloranrosebengal medium and medium for mould and yeasts ­ SIMPLATE. The potato dextrose agar and the dichloranrosebengal medium are routinely employed in analysis laboratories, needing after inoculation, of five days for reading of the results. The medium for mould and yeasts ­SIMPLATE is a new method, based on the metabolic activity, that allows to make the reading in two days after the inoculation, reducing the analytic period significantly. The obtained results were appraised statistically by means of the variance analysis and Test of Tukey. In the laboratory practical the best method is that which recovers larger number of forming colonies units (FCU). In the sample that presented counting of up to 10.000 UFC/g significant difference was not verified among the tested culture mediums, even so in the samples with superior counting to 10.000 UFC/g the dichloranrosebengal medium and the medium SIMPLATE were revealed statistically more effective than the potato dextrose agar.

2020 ◽  
Vol 26 (4) ◽  
pp. 50-55
Author(s):  
A.R. Stasyshyn ◽  
◽  
A.A. Hurayevskyy ◽  
Yu.Y. Holyk ◽  
◽  
...  

Aim. To analyze the effectiveness of a new method of antireflux surgery in patients with hiatal hernia. Materials and Methods. The results of treatment of 157 patients with hiatal hernia from 2016 to 2020 are analyzed. The patients were divided into 3 groups: group I, N=59, underwent laparoscopic antireflux operation modified by the authors (Patent of Ukraine № 59772); group II (N=77), underwent laparoscopic Nissen fundoplication; and group III (N=21) - laparoscopic Toupet fundoplication. Results and Discussion. At 36 months post-surgery follow-up, there was a statistically significant difference in favor of group I on the average scores of the visual analog scale for reflux symptoms, dysphagia and extraesophageal symptoms; the average quality of life questionnaire scores; the average DeMeester index; distribution of the patients by satisfaction; distribution of the patients by degree of reflux esophagitis according to the Los Angeles classification; and distribution by gas-bloat syndrome. Conclusions. Clinical application of the developed new method of laparoscopic surgery for hiatal hernia reduces the number of relapses and complications after surgery, and improves the quality of life of patients. Key words: hiatal hernia, antireflux surgery, new methods of treatment


2021 ◽  
Vol 9 (B) ◽  
pp. 48-54
Author(s):  
Saule Tulegenovna Sapiyeva ◽  
Nurkassi Tulepbergenovich Abatov ◽  
Makash Tynyshtykpaevich Aliyakparov ◽  
Yoshihiro Noso ◽  
Ruslan Muratovich Badyrov ◽  
...  

AIM: A new surgical method of non-tension autoplasty by displaced aponeurotic flap for the treatment of uncomplicated inguinal hernias has been developed. METHODS: Thirty patients were operated on using a new method of autoplasty; the comparison group involved 30 patients which were operated on by Liechtenstein hernioplasty using a partially absorbable mesh implant (UltraPro). The effectiveness of the methods was evaluated by clinical data, instrumental research methods (ultrasound examination, and computed tomography [CT]), and results of life quality. RESULTS: There were no relapse cases in both groups in the early and long-term after surgery. According to ultrasound, inflammatory infiltrate was absent in the group using a new autoplasty method, subclinical seromas resolved earlier after surgery. According to data of compression elastography and CT, the formation of a denser scar in the postoperative area was registered in the comparison group with a statistically significant difference. The feeling of discomfort and pain in the study group was more pronounced in the early post-operative period in comparison with the group using a mesh implant. However, in later terms, the life quality of patients in the group using a new autoplasty method improved with a statistically significant difference. CONCLUSION: The operation method is simple to perform, does not require the use of an endoprosthesis or extensive dissection of tissues and can be a reliable alternative and compete with prosthetic methods of hernioplasty.


2014 ◽  
Vol 39 (3) ◽  
pp. 197-203 ◽  
Author(s):  
Mokhtar Arazpour ◽  
Mohammad A Mardani ◽  
Mahmood Bahramizadeh ◽  
Fereydoun Layeghi ◽  
Fatemeh Zarezadeh ◽  
...  

Background: The use of a finger prosthesis following finger amputation is a common approach that is linked to many factors. Objectives: The aim of this study was to evaluate the effect of a new method of suspension on quality of life, satisfaction, and suspension in these patients. Study design: Quasi-experimental. Method: A total of 24 patients with finger amputation and 12 healthy subjects as control group participated in this study. Two types of finger prostheses with conventional suction suspension and new method suspension were provided. A force gauge was used to evaluate suspension force along with a World Health Organization Quality of Life-BREF questionnaire instrument. Satisfaction of the prosthesis was also evaluated using a visual analog scale. Results: There was significant difference in quality of life assessment between the patient group and healthy control group, but there was no statistical difference ( p > 0.05) between the two patients groups at baseline and after prosthesis use. Using finger prosthesis improved all domains after prosthesis wearing in patients with finger amputations. There were significant differences ( p < 0.05) between the two types of prosthesis in terms of suspension and satisfaction. Conclusion: Wearing the prosthesis with the new method of suspension had an effective role in terms of providing suspension and an increase in satisfaction in patients with finger amputation. Clinical relevance Patients with finger amputation usually use a prosthesis with a simple suspension technique, yet many of these patients experience atrophy and subsequently loss of appropriate suspension. This paper presents a new method of suspension and showed that using this approach improved satisfaction and suspension in patients with finger amputation.


2019 ◽  
Vol 7 (2) ◽  
pp. 82
Author(s):  
Sâmela Leal Barros ◽  
Newton Carlos Santos ◽  
Amanda Priscila da Silva Nascimento ◽  
Mylena Olga Pessoa Melo ◽  
Victor Herbert de Alcântara Ribeiro ◽  
...  

Sunflower almonds are widely marketed and have numerous technological applications. Through the drying process occurs the reduction of the water content of the product, a factor that contributes to increase its useful life. However, nutritional losses and physical damage can also occur. In this context, the objective of this study was to evaluate the effect of different temperatures applied in the drying process on the physical-chemical characteristics of commercial sunflower almonds. Convective drying was performed using temperatures of 40, 50, 60 and 80 °C. Afterwards, the samples before and after the drying process were analyzed with respect to the following parameters: moisture, water activity, ash, lipids, proteins, carbohydrates and energetic value to observe the influence of different drying temperatures on these attributes. It was verified that the increase of the temperature of the drying air causes an increase in the ash content, total solids, lipids, carbohydrates and energetic value. However, the reduction of moisture content, water activity and proteins was observed. There was no significant difference between the samples in relation to pH.


Author(s):  
Talita Fredericci ◽  
Juliana Aparecida Fracarolli ◽  
Pâmela Santaguita Betin ◽  
Lucas Sacramoni Peixoto ◽  
Laila Mami Fukasawa

Spring onion is one of the most used and traded vegetables in the world, essentially with a seasoning nature, it presents high perishability and susceptibility to water loss, which reduces its useful life, making it necessary to use post-harvest techniques, aiming to decrease its metabolic activity and extend its shelf life. The hydrocooling method, which consists of the immersion of the product in a mixture of water and ice, presents itself as a good alternative for the removal of the field heat and to favour the humidity of horticultural goods. The goal of this paper was to check the effects of hydrocooling on the post-harvest conservation and quality of spring onion, aiming to determine the best application time at 5ºC. The method showed efficient, especially on the control of mass loss and on the commercial visual aspects, with the most indicated time of 10 minutes.


2017 ◽  
Vol 20 (4) ◽  
pp. 545-555 ◽  
Author(s):  
Chadi Emil Adamo ◽  
Marina Tomaz Esper ◽  
Gabriela Cunha Fialho Cantarelli Bastos ◽  
Ivone Félix de Sousa ◽  
Rogério José de Almeida

Abstract Objective: To verify, among elderly participants of the University of the Third Age (UnATI) of PUC Goiás, Goiania, Brazil, whether quality of life was higher or lower among veteran or first-year participants of the UnATI, and to evaluate if there was a statistically significant difference between the quality of life of men and women or first-years and veterans. Method: An observational case-control study with a quantitative approach was performed with 100 elderly individuals from the UnATI. Fifty first-years and fifty veterans were involved. Two questionnaires were applied, one with sociodemographic data and the other using the World Health Organization Quality of Life - Old (WHOQOL-OLD) scale, which evaluates the quality of life of the elderly. Variance analysis (ANOVA) and multivariate variance analysis (MANOVA) were performed, with p≤0.05 applied to the relevant statistics. Result: The vast majority of the sample were women (90%), aged between 60 and 86 years old (average of 68.37±5.28 years old). The overall quality of life score was 3.68±0.50 (first-years) and 3.87±0.55 (veterans). There was a statistically significant difference in the areas: sensory functioning (veterans =4.28±0.65 and first-years =3.95±0.80) (p=0.025) and past, present and future activity (veterans =4.0±0.72 and first-years =3.59±0.79) (p=0.008). Multivariate variance analysis identified that the overall quality of life scores of both men (veterans =3.15 and first-years =4.21) and women (veterans =3.78 and first-years =3.86), were highest after they became veteran students (p=0.007). Conclusion: Veterans had a higher quality of life than first-years. The continuing education provided by UnATI has a positive impact on the quality of life of the elderly.


2019 ◽  
Vol 118 (7) ◽  
pp. 1-19
Author(s):  
Geethanjali N ◽  
Parveen Roja M ◽  
Lavanya D

Quality of work life is the major factor to be considered in working environment of any organization. The performance of employees and the organization lies on the ability of the employees based on working environment. The QWL leads to better working environment which improves the performance of organization. The present study has made an attempt to find the level of factors causing QWL and the impact of outcome of QWL in banks. Since the profile of the banks may be associated with the level of outcomes of QWL, the present study has made an attempt to examine it with the help of one way analysis of variance and t-test. The included outcomes of QWL are job satisfaction, job stress, organizational climate, organizational commitment, employees retention behaviour, service quality employees and service productivity of employees. The highly associated determinants of QWL and the significant difference among the PUSBs and PRSBs have been noticed. The significantly associating important profiles of the banks regarding the existence of outcome of QWL are identified.


2020 ◽  
Vol 2020 (4) ◽  
pp. 25-32
Author(s):  
Viktor Zheltov ◽  
Viktor Chembaev

The article has considered the calculation of the unified glare rating (UGR) based on the luminance spatial-angular distribution (LSAD). The method of local estimations of the Monte Carlo method is proposed as a method for modeling LSAD. On the basis of LSAD, it becomes possible to evaluate the quality of lighting by many criteria, including the generally accepted UGR. UGR allows preliminary assessment of the level of comfort for performing a visual task in a lighting system. A new method of "pixel-by-pixel" calculation of UGR based on LSAD is proposed.


2018 ◽  
Vol 8 (6) ◽  
pp. 76-81
Author(s):  
Chu Cao Minh ◽  
Thang Vo Van ◽  
Dat Nguyen Tan ◽  
Hung Vo Thanh

Background: The criteria set of assessing hospital quality in Vietnam in 2016 was revied from the criteria set in 2013 by the Ministry of Health in order to help hospitals to self-assess towards improvinge quality of hospitals in the international integration context. The study aimed to assess the quality of public hospitals in Can Tho City according to the revised criteria set of the Ministry of Health in 2016 and compare the quality among three hospital ranks (including grade I, grade II, and grade III) via to 5 groups of quality criteria. Methods: A cross-sectional study, using secondary data analysis was applied to assess the service quality of 7 general public hospitals in Can Tho City. Results: The average total score of 7 hospitals is 245 and the average for the criteria of 7 hospitals is 2.99, which is just satisfactory. In the criterion of quality, criterion D and E had the lowest scores compared to the other three groups. There was no statistically significant difference (p = 0.076) among the mean scores for the three hospital categories. Conclusion: The quality of public hospitals in Can Tho city in 2016 only reached moderately good level (2.99). Interventions should be developed to improve the quality of hospitals, with particular emphasis on improving the quality of criteria groups D and E. Key words: Quality, hospital, medicine, health, public, Can Tho


2008 ◽  
Vol 47 (01) ◽  
pp. 37-42 ◽  
Author(s):  
T. Pfluger ◽  
V. Schneider ◽  
M. Hacker ◽  
N. Bröckel ◽  
D. Morhard ◽  
...  

SummaryAim: Assessment of the clinical benefit of i.v. contrast enhanced diagnostic CT (CE-CT) compared to low dose CT with 20 mAs (LD-CT) without contrast medium in combined [18F]-FDG PET/CT examinations in restaging of patients with lymphoma. Patients, methods: 45 patients with non-Hodgkin lymphoma (n = 35) and Hodgkin's disease (n = 10) were included into this study. PET, LD-CT and CECT were analyzed separately as well as side-by-side. Lymphoma involvement was evaluated separately for seven regions. Indeterminate diagnoses were accepted whenever there was a discrepancy between PET and CT findings. Results for combined reading were calculated by rating indeterminate diagnoses according the suggestions of either CT or PET. Each patient had a clinical follow-up evaluation for >6 months. Results: Region-based evaluation suggested a sensitivity/specificity of 66/93% for LD-CT, 87%/91% for CE-CT, 95%/96% for PET, 94%/99% for PET/LD-CT and 96%/99% for PET/CE-CT. The data for PET/CT were obtained by rating indeterminate results according to the suggestions of PET, which turned out to be superior to CT. Lymphoma staging was changed in two patients using PET/ CE-CT as compared to PET/LD-CT. Conclusion: Overall, there was no significant difference between PET/LD-CT and PET/CE-CT. However, PET/CE-CT yielded a more precise lesion delineation than PET/LD-CT. This was due to the improved image quality of CE-CT and might lead to a more accurate investigation of lymphoma.


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