scholarly journals Prevalence and Antibiotic Resistance Pattern of Acinetobacter Isolated from Patients Admitted in ICUs in Mazandaran, Northern Iran

2016 ◽  
Vol 8 (11) ◽  
pp. 112 ◽  
Author(s):  
Roya Ghasemian ◽  
Mohammad Ahanjan ◽  
Ebrahim Fatehi ◽  
Mehran Shokri
Keyword(s):  
Antibiotic Resistance ◽  
Resistance Rate ◽  
Test Method ◽  
Diffusion Method ◽  
Resistance Pattern ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Northern Iran ◽  
Cross Sectional ◽  
Antibiotic Resistant

<p><strong>BACKGROUND &amp; PURPOSE: </strong>Antibiotic resistance rate is increasing in <em>Acinetobacter</em> species, especially in <em>Acinetobacter baumannii</em>, as the most important pathogen of hospital and ICU . This research aimed to evaluate antibiotic resistant rate of <em>Acinetobacter</em> spp. isolated from patients admitted to ICUs in educational hospitals affiliated with Mazandaran University of Medical Sciences.</p><p><strong>METHODS:</strong> In this cross-sectional descriptive study, 50 <em>Acinetobacter</em> isolates were collected during 2013- 2014. After confirming <em>Acinetobacter</em> species, antibacterial sensitivity test was done using disc diffusion method and minimal inhibitor concentration (MIC) was evaluated by E-test in all isolates.</p><p><strong>RESULTS:</strong> Disc diffusion method revealed that 100% of isolates were resistant to Amikacin and Cefepim and 96% were resistant to both Meropenem and Ciprofloxacin antibiotics, 6% were sensitive, 18% were intermediate and 76% were resistant to imipenem. Also, 84% of isolates were sensitive and 16% were resistant to colistin. In E-test method, 92% of isolates were sensitive and 8% were resistant to colistin. Moreover, an isolate was sensitive, one was intermediate and the remaining isolates were resistant to ciprofloxacin, and 100% of isolates were resistant to other antibiotics in E-test. Over 96% of <em>Acinetobacter</em> isolates were resistant to the antibiotics frequently used in ICU (ciprofloxacin, meropenem, amikacin, and cefepim). Colistin was found as  the only appropriate antibiotic that could be used for patients in ICU.</p><p><strong>CONCLUSION:</strong> We hope these results could change the attitude of physicians toward using antibiotics in ICUs and encourage them to follow antibiotic stewardship as the only effective strategy to somewhat control antibiotic resistances.</p>

scholarly journals Multidrug resistant Salmonella sp isolated from chicken

2016 ◽  
Vol 4 (1) ◽  
pp. 64
Author(s):  
Atere Victor
Keyword(s):  
Antibiotic Resistance ◽  
Pure Culture ◽  
Multidrug Resistant ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Resistance Level ◽  
Antibiotic Resistant ◽  
Biochemical Characteristics ◽  
Peptone Water

Antibiotic resistance has been a major problem in combatting Salmonella in poultry, this research was designed to determine the antibiotic resistance level of Salmonella isolated from freshly dead chicken. A total of 107 freshly dead chicken were collected from 23 different farms, birds were necropsied, liver and trachea were collected, Salmonella were recovered from the samples using peptone water, Rappaport-Vassiliadis R10 Broth and Salmonella-Shigella agar. Pure culture were identified using cultural, morphological and biochemical characteristics. The pure isolates were subjected to antibiotic test using disc diffusion method. Sixteen isolates of Salmonella were recovered, 3 of which were from the trachea while 13 were recovered from the liver. All of the Salmonella isolates were resistant to Amoxicillin 100% while Nitrofuratoin was least resisted with only 37.5% of the Salmonella isolates showing resistance. The antibiotic resistant pattern often observed in this study were AMP, AUG, TLY with 75% (12); AMP, AUG, TLY, CPR, ENR, 50% (8); while 6.3% (1) was resistant to all of the antibiotics tested. This result showed that there is an emergence of multi-resistance Salmonella in poultry, therefore it is important to carry out sensitivity test before administration of antibiotics in order to control poultry salmonellosis.

scholarly journals The Prevalence and Pattern of Acinetobacter Antibiotic Resistance in the Patients Admitted to Imam Reza Hospital in Kermanshah, Iran (2016 - 2018)

2021 ◽  
Vol In Press (In Press) ◽  
Author(s):  
Marya Shirvani ◽  
Mohammad Hossein Zamanian ◽  
Alireza Janbakhsh ◽  
Babak Sayad ◽  
Siavash Vaziri ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Antimicrobial Agents ◽  
Cross Sectional Study ◽  
Resistance Rate ◽  
Diffusion Method ◽  
Resistance Pattern ◽  
Multiple Drug ◽  
Bacterial Isolates ◽  
Cross Sectional ◽  
Gram Negative

Background: Acinetobacter is a gram-negative coccobacillus, which is widespread in nature and causes several nosocomial infections, such as pneumonia, meningitis, endocarditis, skin and soft tissue infections, conjunctivitis, and bacteremia. Acinetobacter has also demonstrated resistance against multiple antimicrobial agents. Objectives: The present study aimed to investigate the antibiotic resistance pattern of the isolated Acinetobacter strains from the patients admitted to various wards of Imam Reza hospital in Kermanshah, Iran. Methods: This descriptive, cross-sectional study was performed on 726 patients with positive Acinetobacter cultures at Imam Reza hospital during 2016 - 2018. Bacterial isolates were identified using laboratory tests and based on the CLSI protocol, and the standard disc-diffusion method was used assess antibiotic susceptibility. Data analysis was performed in SPSS version 20. Results: Most of the Acinetobacter-positive cases were isolated from the intensive care units (75.88%) and sputum (73.3%) and urine samples (10.1%). In addition, the highest and lowest resistance rate of the isolates was observed against ceftriaxone (96.6%) and ampicillin-sulbactam (58.7%), respectively. Conclusions: According to the results, the bacterial isolates were multiple-drug resistant and showed resistance to ciprofloxacin, ceftazidime, cotrimoxazole, ceftriaxone, cefepime, gentamicin, imipenem, ampicillin, ampicillin-sulbactam, and amikacin. The high resistance to imipenem is rather alarming as it is considered the 'last resort' in the treatment of the infections caused by gram-negative bacteria. Therefore, monitoring programs are recommended to prevent the misuse of this drug in hospitals.

scholarly journals Antibiotic Resistance Pattern of Pseudomonas aeruginosa Isolated from Clinical Specimens from a Tertiary Care Centre in Central Kerala with Special Reference to Carbapenemase Detection

2021 ◽  
Author(s):  
P.M. Anooja ◽  
Anu P. John
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antibiotic Resistance ◽  
Antimicrobial Susceptibility ◽  
Tertiary Care ◽  
Diffusion Method ◽  
Resistance Pattern ◽  
Disc Diffusion ◽  
Tertiary Care Centre ◽  
Cross Sectional ◽  
Extended Spectrum Beta Lactamase

Pseudomonas aeruginosa is inherently resistant to many drugs. It is now an emerging opportunistic pathogen of clinical relevance. The emergence of carbapenemases is another major concern. Initiation of appropriate therapy is of paramount importance thus highlighting the need of active surveillance for newer emerging resistance trends for better infection control. To study the resistance pattern of P. aeruginosa isolates obtained from lab specimens and to determine the production of ESBL and Carbapenemase among them. A hospital-based cross-sectional study was carried out in the Department of Microbiology, Government medical college Thrissur, among P. aeruginosa isolates obtained from lab specimens, from January 2018 – December 2018. 162 isolates were studied. Antimicrobial susceptibility testing was done by Kirby–Bauer disc diffusion method, extended-spectrum beta-lactamase (ESBL) production was confirmed by and phenotypic confirmatory disc diffusion test. Carbapenemase detection was done using the modified carbapenemase inactivation (mCIM) method. The obtained data was analysed. Among 162 isolates 83% were non- multidrug-resistant (MDR) strains and 17% were MDR strains. 22% of ceftazidime resistant isolates were ESBL producers. 6.2% isolates were resistant to imipenem. Among the imipenem resistant isolates, Carbapenemase production was seen in 30% isolates by mCIM test. According to our study, the most effective antibiotic against P. aeruginosa were imipenem and cefoperazone/sulbactam showing resistance in 6.3% and 6.9% isolates respectively. The diversity of antibiotic resistance mechanisms and the emergence of carbapenem resistance is a threat that limits treatment choices. This suggests the need for ongoing antimicrobial susceptibility studies in the future.

scholarly journals Nasal Carriage of Methicillin Resistant Staphylococcus aureus Among Health Care Workers At Tertiary Care Hospital In Dhaka.

2017 ◽  
Vol 11 (2) ◽  
pp. 17-19
Author(s):  
Rahima Akter ◽  
Shikha Paul ◽  
Akhtarun Naher ◽  
Moshiur Rahman ◽  
Sharmin Sultana ◽  
...  
Keyword(s):  
Tertiary Care ◽  
Nasal Carriage ◽  
Healthcare Personnel ◽  
Diffusion Method ◽  
Resistance Pattern ◽  
Dilution Method ◽  
Care Hospital ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Mannitol Salt Agar

Nasal carriage of MRSA among hospital stuff act as a source of endogenous infection and becomes a source for hospital and community acquired infection. The study was conducted to determine the rate antibiotic resistance pattern of nasal carriage of MRSA among the hospital stuff of Sir Salimullah Medical College and Mitford Hospital, Dhaka. Pre moistened nasal swabs from hospital stuff (doctor, nurses, lab technicians and other helping stuff were obtained. These swabs were inoculated into Blood agar and Mannitol salt agar media. Antibiogram was done by modified Kirby Bauer disc diffusion method. MRSA were detected by oxacillin and cefoxitin disc diffusion method. The resistance was confirmed by MIC of oxacillin agar dilution method. Out of 142 samples 34 strains of Staphylococcus were isolated among them 07 (4.93%) were MRSA and 27 (19.01%) were MSSA. The carriage rate of MRSA was higher among nurse than other healthcare provider. Nasal carriage of MRSA is responsible for spreading infection from healthcare personnel to normal individual. So, regular screening of carrier is required from prevention of hospital acquired infection. Bangladesh J Med Microbiol 2017; 11 (2): 17-19

In vitro sensitivity of Hungarian Actinobaculum suis strains to selected antimicrobials

2003 ◽  
Vol 51 (1) ◽  
pp. 53-59 ◽  
Author(s):  
I. Biksi ◽  
Andrea Major ◽  
L. Fodor ◽  
Keyword(s):  
Type Strain ◽  
Diffusion Method ◽  
Resistance Pattern ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Antimicrobial Sensitivity ◽  
Variable Sensitivity ◽  
Agar Dilution ◽  
Almost All

In vitro antimicrobial sensitivity of 12 Hungarian isolates and the type strain ATCC 33144 of Actinobaculum suis to different antimicrobial compounds was determined both by the agar dilution and by the disc diffusion method. By agar dilution, MIC50 values in the range of 0.05-3.125µg/ml were determined for penicillin, ampicillin, ceftiofur, doxycycline, tylosin, pleuromutilins, chloramphenicol, florfenicol, enrofloxacin and lincomycin. The MIC50 value of oxytetracycline and spectinomycin was 6.25 and 12.5µg/ml, respectively. For ofloxacin, flumequine, neomycin, streptomycin, gentamicin, nalidixic acid, nitrofurantoin and sulphamethoxazole + trimethoprim MIC50 values were in the range of 25-100µg/ml. With the disc diffusion method, all strains were sensitive to penicillin, cephalosporins examined, chloramphenicol and florfenicol, tetracyclines examined, pleuromutilins, lincomycin and tylosin. Variable sensitivity was observed for fluoroquinolones (flumequine, enrofloxacin, ofloxacin), most of the strains were susceptible to marbofloxacin. Almost all strains were resistant to aminoglycosides but most of them were sensitive to spectinomycin. A strong correlation was determined for disc diffusion and MIC results (Spearman's rho 0.789, p<0001). MIC values of the type strain and MIC50 values of other tested strains did not differ significantly. Few strains showed a partially distinct resistance pattern for erythromycin, lincomycin and ampicillin in both methods.

scholarly journals Determination of antibiotic resistance and minimum inhibitory concentration of meropenem and imipenem growth in Klebsiella strains isolated from urinary tract infection in Shahrekord educational hospitals

2019 ◽  
Vol 21 (2) ◽  
pp. 80-85
Author(s):  
Farshad Kakian ◽  
Behnam Zamzad ◽  
Abolfazl Gholipour ◽  
Kiarash Zamanzad
Keyword(s):  
Antibiotic Resistance ◽  
Urinary Tract ◽  
Minimum Inhibitory Concentration ◽  
Bacterial Infections ◽  
Inhibitory Concentration ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Accurate Identification ◽  
Tract Infections

Background and aims: Klebsiella is an opportunistic organism that is the cause of severe diseases such as pneumonia, septicemia, and urinary tract infections (UTIs). In addition, high antibiotic resistance has challenged the treatment of this bacterium. However, carbapenem antibiotics are considered as the therapeutic agents for selecting the treatment of penicillin- and cephalosporin-resistant gram-negative bacterial infections. The present study aimed to determine the resistance and minimum inhibitory concentration (MIC) of meropenem and imipenem. Methods: A total of 80 Klebsiella spp isolated from UTIs were collected in various educational wards (i.e., urology, obstetrics, and gynecology, as well as the units of infectious diseases, internal medicine, and intensive care) in different hospitals of Shahrekord. The isolates were then identified by using biochemical tests. Further, disc diffusion method was employed to determine the antibiotic resistance. Furthermore, MIC was estimated by the Epsilon-test strip. Moreover, P=Q=0.50, an error of 0.05, and an accuracy of 0.11 were considered for determining the sample size (n=80). Results: Based on the results of disc diffusion method, 24 strains were resistant to meropenem and imipenem. Additionally, the MIC was 24 (30%) by the E-test. In addition, 24 isolates had a MIC of ≥4 μg/mL for meropenem and imipenem and thus were resistant while 18 isolates were found to have a MIC of 1≤ MIC<4 μg/mL and therefore, were considered semi-sensitive (P<0.001). Conclusion: In general, Klebsiella strains were found to be resistant to meropenem and imipenem. Therefore, rapid and accurate identification of these strains and the selection of appropriate antibiotics can help quickly eradicate the infections caused by these bacteria. Accordingly, a waste of time, the consumption of medication, or even an increased resistance are prevented.

scholarly journals Gölbaşı ve Azaplı Göllerinden (Adıyaman) İzole Edilen Bakterilerin Tiplendirilmesi ve Çoklu Antibiyotik Dirençliliklerinin Araştırılması

2017 ◽  
Vol 5 (1) ◽  
pp. 43
Author(s):  
Fikret Büyükkaya Kayış ◽  
Sadık Dinçer ◽  
Fatih Matyar ◽  
Hatice Aysun Mercimek Takcı ◽  
Melis Sümengen Özdenefe ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Seasonal Changes ◽  
Reference Value ◽  
Summer Season ◽  
Diffusion Method ◽  
Gram Negative Bacteria ◽  
Disc Diffusion ◽  
Multiple Antibiotic Resistance ◽  
Disc Diffusion Method ◽  
Gram Negative

Identification and multiple antibiotic resistances of amphicillin, chloramphenicol, streptomycin and tetracycline resistant gram-negative bacteria that isolated microorganisms from Gölbaşı and Azaplı lakes (Adiyaman) were investigated in this study. Seasonally taken isolates of totally 386 bacteria in 10 different species from 7 genera were scanned against 16 antibiotics [gentamycin, imipenem, kanamycin, chloramphenicol, meropenem, nalidixic acid, nitrofurantoin, penicillin, cephalothin, cefazolin, cefpirome, ceftizoxime, cefuroxime, streptomycin, tetracycline and trimethoprim-sulphamethoxazole (Bioanalyse)] by using the disc diffusion method to determine the prevalence of multiple antibiotic resistance. Multiple antibiotic resistance of stations showed seasonal changes between 0.29 and 0.91. In generally, multiple antibiotic resistance in Golbasi and Azapli lakes were higher than the reference value and highest multiple antibiotic resistance values were obtained at summer season (3th period). When the obtained data are considered, high multiple antibiotic resistance poses a risk in terms of public health and for economically important animals.

scholarly journals Antibiotic Resistance of Helicobacter Pylori and Influencing Factors in Yangzhou, China: a Cross-sectional Study

2021 ◽  
Author(s):  
Yun Zhang ◽  
Xinyi Feng ◽  
Lijun Bian ◽  
Yan Zhang ◽  
Qian Li ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Antibiotic Resistance ◽  
Gastrointestinal Symptoms ◽  
Cross Sectional Study ◽  
Resistance Rate ◽  
Resistance Pattern ◽  
Cross Sectional ◽  
Chip Technology ◽  
Resistance Patterns ◽  
H Pylori

Abstract Background: The Helicobacter pylori (H. pylori) antibiotic resistance pattern differs geographically, knowledge of local antimicrobial resistance pattern is key to successful eradication. The current study was performed to investigate the resistance of H. pylori to 5 commonly used antibiotics in Yangzhou.Methods: A total of 461 H. pylori strains were collected from April 2018 to September 2019 in Yangzhou and tested for their susceptibility to clarithromycin, levofloxacin, metronidazole, amoxicillin, tetracycline using gene chip technology.Results: The resistance rates of H. pylori to clarithromycin, levofloxacin, metronidazole, amoxicillin and tetracycline were 41.0%, 44.9%, 38.8%, 6.3% and 1.1%. In addition, sixteen multiple resistance patterns were detected, and fortunately there were no strains resistant to all five antibiotics. The vacA s1 allele was detected in each strain, 64.0% of the isolates were s1/m2 genotype, 36.0% s1/m1 genotype. In multivariate analysis, history of chronic superficial gastritis was significantly associated with clarithromycin resistance, strains isolated from subjects with gastrointestinal symptoms had a significantly higher likelihood of metronidazole resistance, and antibiotic resistance of H. pylori was independent of both sex and age.Conclusion: The resistance rate of H. pylori to clarithromycin, levofloxacin and metronidazole were very high in Yangzhou, the results will help in selecting effective eradication regimens.

scholarly journals Health Care Associated Multidrug Resistant Acinetobacter Infections in a Tertiary Critical Care Center of Dhaka, Bangladesh

2020 ◽  
Vol 38 (2) ◽  
pp. 59-63
Author(s):  
Md Faruk Mia ◽  
Arif Ahmed Khan ◽  
Anjana Chakraborty
Keyword(s):  
Health Care ◽  
Critical Care ◽  
Care Center ◽  
Resistance Rate ◽  
Diffusion Method ◽  
Multi Drug Resistance ◽  
Military Hospital ◽  
Resistance Pattern ◽  
Cross Sectional ◽  
High Dependency

  Introduction: Acinetobacter spp, emerged as important pathogen exhibiting increasing antimicrobial resistance. Worldwide, multi-drug resistant Acinetobacter is being established as a significant cause of health-care-associated infections along with their resistant trait. Methods:This retrospective, cross-sectional study was done in critical care centers (CCC) of Combined Military Hospital (CMH) from May to September 2016. Total 69 patients with infection by Acinetobacter were included in this study. Center for Disease Control (CDC) criteria for selection of nosocomial infection were used. Specimen were inoculated in blood and MacConkey agar, incubated 24 hours at 37°C in aerobic condition and yield in plates were examined. Antibiotic resistance of isolated organisms were observed in Mueller Hinton agar sensitivity media by using Kirby Bauer disc diffusion method. Results:Among the total 69 (males 27, females 42,) and age range 2-75 years, Acinetobacter infected patients, 31 were from medical, 23 from surgical and 15 were from high dependency unit (HDU). Specimens were tracheal aspirate (24, 34.80%), urine (18, 26.90%), wound swab (12, 17.40%), pus (6, 8.70%), high vaginal swab (6, 8.70%) and bronchoalveolar lavage (3, 4.35%). Empiric antibiotics were given in all, one course of antibiotic was used in one, in others two or more courses were given. In the study, total 17 antibiotics were used for identifying resistance pattern of Acinetobacter isolates. Ceftazidime (91.30%), ceftriaxone (91.30%), amikacin(52.20%), gentamicin (33.33%), cotrimoxazole (50%) and ciprofloxacin (65.22%) were mostly resistant. Colistin appeared as most effective antibiotic with only 7.70% resistance rate. Conclusion: Acinetobacter, isolated in this study were resistant to most antibiotics. Colistin was the most sensitive one. Multi drug resistance (DR), extended DR and pan DR Acinetobacter organisms are reported worldwide, work on clinical presentation, trends of drug use in particular set up is important to combat the situation of ‘Going out of drug state’. J Bangladesh Coll Phys Surg 2020; 38(2): 59-63

scholarly journals Phylogenetic Groups ofEscherichia coliStrains from Patients with Urinary Tract Infection in Iran Based on the New Clermont Phylotyping Method

2015 ◽  
Vol 2015 ◽  
pp. 1-7 ◽  
Author(s):  
Darioush Iranpour ◽  
Mojtaba Hassanpour ◽  
Hossein Ansari ◽  
Saeed Tajbakhsh ◽  
Gholamreza Khamisipour ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Cross Sectional Study ◽  
Diffusion Method ◽  
Resistance Pattern ◽  
Multiple Drug ◽  
Phylogenetic Groups ◽  
Disk Diffusion Method ◽  
Cross Sectional ◽  
E Coli ◽  
Pcr Method

Objectives.In 2013, Clermont classifiedE. colistrains into eight phylogenetic groups using a new quadruplex PCR method. The aims of this study were to identify the phylogenetic groups ofE. colibased on this method and to assess their antibiotic resistance patterns in Bushehr, Iran.Methods. In this cross-sectional study, 140E. coliisolates were subjected to phylogenetic typing by a quadruplex PCR method. Antimicrobial susceptibility testing was performed by disk diffusion method.Results. Phylogenetic group B2 was most predominant (39.3%), followed by unknown (27.1%), E (9.3%), C and clade I (each 6.4%), B1 (5%), F and D (each 2.9%), and A (0.7%). The most common antibiotic resistance was related to amoxicillin (82.1%) and the least to meropenem (0.7%). 82.14% of isolates were multiple drug resistant (MDR). Antibiotic resistance was mainly detected in group B2 (50%).Conclusions.Our findings showed the high prevalence of MDRE. coliisolates with dominance of group B2. About 25% ofE. coliisolates belong to the newly described phylogroups C, E, F, and clade I. Such studies need to be done also in other regions to provide greater understanding of the antibiotic resistance pattern and the prevalences of different phylogenetic groups.

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