scholarly journals In vivo Anti-Cancer Activity of N-halamines

2016 ◽  
Vol 9 (1) ◽  
pp. 20
Author(s):  
Faten Zahran ◽  
Akaber T. Keshta ◽  
Abd El-Shafey I. Ahmed
Keyword(s):  
Ehrlich Ascites Carcinoma ◽  
Positive Control ◽  
Positive Control Group ◽  
Control Group ◽  
In Vivo Antitumor Activity ◽  
Ehrlich Ascites ◽  
Anti Cancer ◽  
Anti Oxidant ◽  
Copolymer 1

Purpose: N-halamines were known for their antimicrobial action due to the presence of halogen in their structure. In our search for new anti-cancer agents, we have evaluated the anti-cancer and anti-oxidant properties of some synthetic N-Halamines with high and low molecular weight in comparison with their non-halogenated forms. Urea epichlorohydrin copolymer (1), 4(1H, 3H-2, 6-dioxo-1, 3, 5-trizenyl)-O-iminomethylpolyethylene (3) and cynuric acid (5) in addition to their halogenated forms 2, 4 and 6 were selected for this study. Methodology: the toxicity for the synthesized compounds was determined. The anti-cancer and anti-oxidant activities were studied by evaluation the viability of tumor cells, life span prolongation, and estimation of antioxidants, and effects of these compounds on liver histology. Findings: Doses up to 2000 mg/kg indicated good safety in all investigated compounds. In Vivo antitumor activity results against Ehrlich ascites carcinoma (EAC) cells for the investigated compounds revealed that, the volume of ascites was significantly decreased in compounds 1, 2, 3, 4, 5 and 6 treated groups. EAC cell count was significantly reduced for similar groups, respectively, compared to the positive control group. Malonadildehyde, and nitric oxide showed a significant reduction in their levels in the treated groups, while catalase showed a significant elevation in its activity in the same groups compared to positive control group. Conclusion: Halogenated compounds showed good anti-oxidant behavior while compound 6 showed the best anti-tumor effect.

scholarly journals Biochemical Studies on Coumarin Derivatives as Antioxidant and Antitumor Agents

2019 ◽  
pp. 1-16
Author(s):  
F. Z. Mohammed ◽  
I. M. EL-Deen ◽  
A. S. Tmamm
Keyword(s):  
Antitumor Agents ◽  
Antioxidant Activities ◽  
Bromine Atom ◽  
Tumor Model ◽  
Positive Control ◽  
Positive Control Group ◽  
Control Group ◽  
Coumarin Derivatives ◽  
Anti Oxidant

Background: Coumarin derivatives have attracted intense interest in recent years, because they have anti-tumor, antioxidant activities, and induce apoptosis. Aims and Objective: Our study aims to evaluate the antitumor and anti-oxidant activities of new Coumarin derivatives: N-(P-chlorophenyl)-7-hydroxycoumarin-3-yl carboxamide and Ethyl 7-hydroxycoumarin-3-yl ester against in vivo tumor model. Methodology: the toxicity for the synthesized compounds was determined. The anticancer and anti-oxidant activities were studied by evaluation the viability of tumor cells, life span prolongation, and estimation of antioxidants. Results: Our compounds exhibited significant anti-oxidant activity towards Ehrlich ascites carcinoma (EAC) cells by reduction the MDA and NO concentration (p<0.001) compared to the positive control group. Whereas significantly increase in the G. peroxidase activity (p<0.001) in treated groups compared to the positive control group. Anticancer agent kills tumors by induction of apoptosis that showing significantly increases in Caspase-3 and Bax activity compared to positive control group. Discussion: The compound N-(P-chlorophenyl)-7-hydroxycoumarin-3-yl carboxamide is better than Ethyl 7-hydroxycoumarin-3-yl ester compound because of the nature of the halogen atom (a chlorine or a bromine atom) in the ‘meta’ position of the phenyl ring relative to the ester oxygen atom of 2-oxo-2H-1-benzopyran- 3-carboxylate led to a better anti-tumor effect than that observed in the absence of any substituent.

scholarly journals An Explanation of the Underlying Mechanisms for the In Vitro and In Vivo Antiurolithic Activity of Glechoma longituba

2016 ◽  
Vol 2016 ◽  
pp. 1-11 ◽  
Author(s):  
Qiang Liang ◽  
Xiaoran Li ◽  
Wangning Zhou ◽  
Yu Su ◽  
Shenbao He ◽  
...  
Keyword(s):  
Kidney Injury ◽  
Positive Control ◽  
Potassium Citrate ◽  
Positive Control Group ◽  
Control Group ◽  
Protective Effects ◽  
In Vivo Models ◽  
Glechoma Longituba

Purpose. To use in vitro and in vivo models to evaluate Glechoma longituba extract to provide scientific evidence for this extract’s antiurolithic activity. Materials and Methods. Potassium citrate was used as a positive control group. Oxidative stress (OS) markers and the expression of osteopontin (OPN) and kidney injury molecule-1 (KIM-1) were measured to assess the protective effects of Glechoma longituba. Multiple urolithiasis-related biochemical parameters were evaluated in urine and serum. Kidneys were harvested for histological examination and the assessment of crystal deposits. Results. In vitro and in vivo experiments demonstrated that treatment with Glechoma longituba extract significantly decreased calcium oxalate- (CaOx-) induced OPN expression, KIM-1 expression, and OS compared with the positive control group (P<0.05). Additionally, in vivo rats that received Glechoma longituba extract exhibited significantly decreased CaOx deposits and pathological alterations (P<0.05) compared with urolithic rats. Significantly lower levels of oxalate, creatinine, and urea and increased citrate levels were observed among rats that received Glechoma longituba (P<0.05) compared with urolithic rats. Conclusion. Glechoma longituba has antiurolithic effects due to its possible combined effects of increasing antioxidant levels, decreasing urinary stone-forming constituents and urolithiasis-related protein expression, and elevating urinary citrate levels.

scholarly journals Efek Gel Ekstrak Kulit Buah Manggis (Garcinia Mangostana) pada Perlekatan Komposit pasca In-Office Bleaching

2015 ◽  
Vol 1 (1) ◽  
pp. 32
Author(s):  
Farah Amiria ◽  
Harwoko Harwoko ◽  
A. Haris Budi Widodo
Keyword(s):  
Ascorbic Acid ◽  
Bond Strength ◽  
Shear Bond Strength ◽  
Positive Control ◽  
Negative Control ◽  
Positive Control Group ◽  
Control Group ◽  
Garcinia Mangostana ◽  
Anti Oxidant ◽  
Resin Bonding

Hidrogen peroksida pada perawatan in-office bleaching meninggalkan residu yang dapat menghambat polimerisasi pada sistem resin bonding. Asam askorbat 10% sebagai antioksidan dapat mengeliminasi residu peroksida sebelum proses penumpatan. Kulit buah manggis (Garcinia mangostana) memiliki aktivitas antioksidan yang berpotensi untukmenggantikan peran asam askorbat. Penelitian ini bertujuan untuk membuktikan efek gel ekstrak kulit buah manggis dalam memperbaiki perlekatan komposit pada gigi pasca in-office bleaching. Penelitian ini merupakan penelitian eksperimental laboratorium. Sebanyak 25 gigi premolar pertama rahang atas dilakukan in-office bleaching danselanjutnya dibagi menjadi 5 kelompok terdiri dari kontrol negatif (tanpa agen antioksidan), kontrol positif (asam askorbat 10%), dan kelompok perlakuan gel ekstrak kulit buah manggis 10%, 20%, dan 40%. Perlekatan komposit ditentukan dengan uji geser, dilanjutkan pengamatan mikrostruktur permukaan email. Hasil penelitian menunjukkan nilai perlekatan komposit pada kelompok kontrol negatif dan kontrol positif berturut-turut sebesar 25,82±2,88 Mpa dan 37,68±1,06 Mpa. Pada kelompok perlakuan gel ekstrak kulit manggis 10%, 20%, dan 40% diperoleh nilai perlekatan berturut-turut sebesar26,54±2,68; 32,64±2,33; dan 57,34±1,51 Mpa. Nilai perlekatan komposit pada pemberian gel ekstrak kulit manggis 20% dan 40% berbeda signifikan dibandingkan dengan kontrol negatif maupun kontrol positif (p<0,05). Kenaikan konsentrasi gel ekstrak kulit manggis memberikan peningkatan jumlah fraktur email. Gel ekstrak kulit manggis 20% dan 40% dapat memperbaiki perlekatan komposit sehingga berpotensi sebagai agen antioksidan pada gigi pasca in-office bleaching. Effect of Mangosteen Gel on Shear Bond Strength Of Composite Post In-Office Bleaching. The residual peroxide after in-office bleaching inhibits the polymerization of the resin bonding systems. Ascorbic acid (10%) as anti-oxidant agent has a potential to remove the residual peroxide before restoration procedure. Mangosteen extracthas an anti-oxidant potential that can be used to remove the residual peroxide. The aim of the study is to prove the effect of mangosteen extract gel to improve shear bond strength of composite post in-office bleaching. The experimental laboratory design was implemented to this research. The study was carried out on 25 maxillary first premolars thathad been applied in-office bleaching procedure. The teeth were divided into five groups as follow: negative-control group (without anti-oxidant agent), positive-control group (10% ascorbic acid), and the treatment groups (10%, 20%, and 40% mangosteen extract gel). Shear bond strength of the specimens was tested and followed by microstructuretesting on email. The results show that negative-control and positive-control group scores were 25,82±2,88 Mpa and 37,68±1,06 Mpa, followed by the scores of 10%, 20%, and 40% mangosteen extract gel groups which were 26,54±2,68; 32,64±2,33; and 57,34±1,51 Mpa respectively. Significant higher shear bond strength values were observed in 20% and 40% mangosteen extract gel groups compared to the negative-control and positive-control group (p<0,05). The increasing concentration of mangosteen extract gel provided increasing number of email fractures. Mangosteen extract gel 20% and 40% improve the shear bond strength of composite, confirming its potential as an antioxidant agent for the teeth post in-office bleaching.

scholarly journals PENGARUH PEMBERIAN GEL AIR KELAPA GADING (COCOS CAPITATA) TERHADAP TINGKAT KECEMASAN PADA HIPERSENSITIVITAS DENTIN Studi in vivo terhadap tikus wistar jantan

2016 ◽  
Vol 3 (2) ◽  
pp. 133
Author(s):  
Chariza Hanum Mayvita Iskandar ◽  
Yayun Siti Rochmah ◽  
Arlina Nurhapsari
Keyword(s):  
Treatment Group ◽  
Wistar Rats ◽  
Positive Control ◽  
Anxiety Level ◽  
Positive Control Group ◽  
Control Group ◽  
Dentin Hypersensitivity ◽  
Group I ◽  
Significant Difference

Background: Ivory palm water (Cocos capitata) has a high potassium content. Potassium ion can depolarized excited dental nerve on dentin hypersensitivity. This study was to determine the effect of water gel ivory palm against dentine hypersensitivity by the anxiety level of mice using RGS (Rat Grimace Scale).Method: This research has been through ethical clearance which researchmethod was quasy experimental in vivo. The sample was 16 Wistar rats (Rattus novergicus) male 4-5 months with 4 mice in each group. Group I as a positive control. Group II as the ivory palm water gel 10% treatment group. Group III as the ivory palm water gel 70% treatment group. Group IV as a negative control. Research data analysis used Kruskal Wallis test with p <0.05 then continued by Mann Whitney test.Result: Kruskal Wallis test obtained significant difference between the four study groups (p <0.05). Mann Whitney test results in comparison either between positive control group and ivory palm water gel 10% and 70%, and comparison group of ivory palm water gel 10% and 70% weren’t significantly different.Conclusion: This study conclusion there was no significant difference between ivory palm water gel 10 % and 70 % in reducing anxiety level in male Wistar rats with dentinal hypersensitivity

scholarly journals Anti-Oxidant and Hepatoprotective Effects of Senecio biafrae on CCl4-induced Liver Damage in Rats

2019 ◽  
Vol 13 (2) ◽  
pp. 31-35
Author(s):  
Israel Oghenevwodoko Okoro ◽  
◽  
Helen Ejiro Kadiri ◽  
Keyword(s):  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Albino Rats ◽  
Root Extract ◽  
Distilled Water ◽  
Standard Drug ◽  
Anti Oxidant

Background: The present study was performed to explore whether the aqueous extract of Senecio biafrae (S. biafrae) roots provide any in vivo protective activity against carbon tetrachloride (CCl4)-induced hepatotoxicity in male albino rats. Methods: Rats (150-200 grams) were grouped into five groups (A-E) of six rats each and were treated orally for twelve days with 72 hourly administration of CCl4 (1 mL/kg) as follows: Group A received distilled water only (negative control), Group B was administered distilled water plus CCl4 (positive control), Group C was administered 400 mg/kg extract and CCl4, Group D received 200 mg/extract and CCl4, while Group E was administered standard drug (Silymarin 25mg/kg, PO). Results: Pre-treatment with the extract of S. biafrae (200 or 400mg/kg) or Silymarin (25mg/kg) caused significant restoration in the biomarkers as evaluated by reducing the levels of malondialdehyde, transaminases and elevating the levels of superoxide dismutase, catalase and glutathione peroxidase activities, which were altered by CCl4 toxicity. The extract at a dose of 400mg/kg demonstrated similar activities comparable to the standard drug (Silymarin). Conclusion: The results of this study indicate that the root extract of S. biafrae possesses hepatoprotective and anti-oxidant properties which may be due to the presence of phytochemicals in it.

scholarly journals Potency of Sunkist Orange (Citrus sinensisL. Osbeck) against Kidney Histology of White Wistar Rats Induced by Gentamicin

2020 ◽  
Vol 25 (1) ◽  
pp. 42
Author(s):  
Masdalena Nasution ◽  
Chrismis Novalinda Ginting ◽  
Edy Fachrial ◽  
I Nyoman Ehrich Lister
Keyword(s):  
Citrus Sinensis ◽  
Cell Damage ◽  
Ethanol Extract ◽  
Food Supplement ◽  
Positive Control ◽  
Positive Control Group ◽  
Control Group ◽  
Anti Cancer ◽  
Nephroprotective Activity ◽  
Histopathological Results

About 1.7%-58% of all cases of acute kidney failure are caused by gentamicin nephrotoxicity and consequently increasing urea and creatinine levels in the blood which are indications of damage to function. Oranges contain secondary metabolites such as flavonoids, alkaloids, coumarin, limonoid, keratinoid and essential oil that have pharmacological activities such as antioxidants, anti-inflammatory, anti-cancer, and also nephroprotector. The purpose of this study is to evaluate the nephroprotective activity of Citrus sinensis peel ethanol extract (EEKJS) on rats induced by gentamicin. Rats were induced using gentamicin at a dose of 80 mg/kgBW intraperitoneally on the 7th day after induced at a dose of 50 mg/kgBW, 100 mg/kgBW, and 200 mg/kgBW for 7 days. The results obtained from the ethanol extract of Citrus sinensis peel gave nephroprotective with the lowest serum creatinine and urea levels at a dose of 200 mg/kgBW which was 0.4±0.02 mg/dl and 43.33 ± 2.51 mg/dl and difference significantly (p<0.05) with a positive control group which was only induced by gentamicin and histopathological results showed significant cell damage in the positive control group that was only induced by gentamicin, and in the 50 mg/kgBW dose group, 100 mg/kgBW, 200 mg/kgBW had cell repair after gentamicin induction. Citrus sinensis are highly recommended to be a food supplement for kidney protection.

scholarly journals Salvia officinalis L. extract increase the antitumor effect of Doxorubicin on Ehrlich carcinoma tumor cells

2019 ◽  
Vol 76 (1) ◽  
pp. 81
Author(s):  
Roxana Liana STAN ◽  
Eleonora MARIAN ◽  
Bogdan SEVASTRE ◽  
Orsolya SÁRPATAKI ◽  
Mariana MUREȘAN ◽  
...  
Keyword(s):  
Tumor Development ◽  
Tumor Model ◽  
Ehrlich Ascites Carcinoma ◽  
Salvia Officinalis ◽  
Ehrlich Carcinoma ◽  
Tumor Control ◽  
In Vivo Antitumor Activity ◽  
Ehrlich Ascites ◽  
Salvia Officinalis L

The current study demonstrated the superior in vivo antitumor activity of the association between Salvia officinalis L. and Doxorubicin as compared with Doxorubicin alone and its lack of toxicity. The study was carried out on 32 Mus musculus female mice, for 14 days long. The tumor model was Ehrlich Ascites Carcinoma (EAC). Animals were divided in four equal groups of 8 mice: normal control, tumor control, EAC + doxorubicin and EAC + doxorubicin + Salvia officimalis L. All the values that reflect the tumor development (difference in body weight, EAC volume and cellular concentration) were improved in significant manner. Hematological and biochemistry parameters determination were performed for all four groups of mice and no cytotoxicity was found. The efficacy of Salvia officinalis L. as an adjuvant therapy in cancer has been demonstrated.

scholarly journals Synthesis, Characterization, and In Vivo Anti-Cancer Activity of New Metal Complexes Derived from Isatin-N(4)antipyrinethiosemicarbazone Ligand Against Ehrlich Ascites Carcinoma Cells

Molecules ◽  
2019 ◽  
Vol 24 (18) ◽  
pp. 3313 ◽  
Author(s):  
El-Saied ◽  
El-Aarag ◽  
Salem ◽  
Said ◽  
Khalifa ◽  
...  
Keyword(s):  
Metal Complexes ◽  
Solid Tumors ◽  
Tumor Volume ◽  
Ehrlich Ascites Carcinoma ◽  
Ehrlich Ascites ◽  
Anti Cancer ◽  
Caspase 7 ◽  
The Impact ◽  
Eac Cells

The current study aimed to synthesize new metal coordination complexes with potential biomedical applications. Metal complexes were prepared via the reaction of isatin-N(4)anti- pyrinethiosemicarbazone ligand 1 with Cu(II), Ni(II), Co(II), Zn(II), and Fe(III) ions. The obtained metal complexes 2−12 were characterized using elemental, spectral (1H-NMR, EPR, Mass, IR, UV-Vis) and thermal (TGA) techniques, as well as magnetic moment and molar conductance measurements. In addition, their geometries were studied using EPR and UV−Vis spectroscopy. To evaluate the in vivo anti-cancer activities of these complexes, the ligand 1 and its metal complexes 2, 7 and 9 were tested against solid tumors. The solid tumors were induced by subcutaneous (SC) injection of Ehrlich ascites carcinoma (EAC) cells in mice. The impact of the selected complexes on the reduction of tumor volume was determined. Also, the expression levels of vascular endothelial growth factor (VEGF) and cysteine aspartyl-specific protease-7 (caspase-7) in tumor and liver tissues of mice bearing EAC tumor were determined. Moreover, their effects on alanine transaminase (ALT), aspartate transaminase (AST), albumin, and glucose levels were measured. The results revealed that the tested compounds, especially complex 9, reduced tumor volume, inhibited the expression of VEGF, and induced the expression of caspase-7. Additionally, they restored the levels of ALT, AST, albumin, and glucose close to their normal levels. Taken together, our newly synthesized metal complexes are promising anti-cancer agents against solid tumors induced by EAC cells as supported by the inhibition of VEGF and induction of caspase-7.

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