scholarly journals Correlations and Path Analysis in Segregating Cowpea Generations Regarding Biological Nitrogen Fixation

2022 ◽  
Vol 14 (2) ◽  
pp. 79
Author(s):  
Ismael Gaião da Costa ◽  
José Wilson da SiIva ◽  
Gheysa Coelho Silva ◽  
Mario de Andrade Lira Junior ◽  
Cybelle Souza de Oliveira ◽  
...  

The objective of this research was to evaluate the correlations between variables related to the biological nitrogen fixation (BNF) in segregating generations of cowpea and to unfold these correlations in direct and indirect effects, through path analysis. An outdoor bench experiment was conducted at Carpina Experimental Sugarcane Station of, located at the Zona da Mata region of the State of the Pernambuco, Northeast of Brazil (Federal Rural University of Pernambuco), between March and April 2016. The seeds were planted in 20 cm × 30 cm polyethylene bags, using a substrate composed of a mixture of vermiculite and sand washed in a ratio of 1:1. Bradyrhizobium references, recommended for culture, were used as a mixture of two strains. Parental and F2, F3 and F4 generations were evaluated in a randomized block design with four replicates. Data collection was performed 45 days after the emergency (DAE). Phenotypic correlations and path analysis of the number of nodules per plant (NN), nodules dry mass (NDM), dry roots mass (DRM), dry mass of aerial part (DMAP), nodulation efficiency (NODE) and nitrogen accumulated in the aerial part (NAAP). The phenotypic correlations between the variables related to the BNF showed high magnitudes, demonstrating that there is a great influence of each of the variables on the others, furthermore the path analysis of the coefficients indicated that all the primary components (NN, NDM, DRM, DMAP and NODE) must be considered when it is desired to increase the NAAP in segregating generations of cowpea.

Author(s):  
Fernando S. Galindo ◽  
Marcelo C. M. Teixeira Filho ◽  
Salatier Buzetti ◽  
Mariana G. Z. Ludkiewicz ◽  
Poliana A. L. Rosa ◽  
...  

ABSTRACT Biological nitrogen fixation (BNF) efficiency can be increased by co-inoculation with bradyrhizobia and Azospirillum brasilense, allowing even greater uptake of water and nutrients, leading to higher yields. Thus, this study aimed to evaluate the technical and economic viability of soybean in the Cerrado, according to the cultivars and co-inoculation with Azospirillum brasilense. The experiment was conducted in Selvíria, MS, in no-tillage system, in Oxisol, arranged in a randomized block design in a 2 x 2 factorial scheme with two cultivars (‘Potência’ and ‘Valiosa’), with and without co-inoculation with Azospirillum brasilense in the seed. Co-inoculation with A. brasilense increases grain yield in the cultivars ‘Potência’ and ‘Valiosa’, being economically viable. However, using the cultivar ‘Potência’ co-inoculated led to the highest profitability.


2019 ◽  
Vol 35 (4) ◽  
Author(s):  
Cassiele Uliana Facco ◽  
Carlos Alberto de Bastos Andrade ◽  
Guilherme Fernando Capristo Silva ◽  
Carlos Antonio da Silva Junior ◽  
Ana Claudia Sossai Souza

The growing quest for sustainability in agricultural production systems has been pushing researchers to develop new technologies under different soil and climatic conditions. Based on this, and knowing the socioeconomic importance of the bean, the use of biological nitrogen fixation (BNF) is explored. Thus, the quest for quantitative knowledge of BNF at the phenological stages in a new bean line using promising and commercial strains becomes necessary. The objective of this study was to evaluate the strains UFLA 02 100 and CIAT 899 in the different phenological stages of the LEP 02 11 common bean strain using the biological nitrogen fixation. The experiment was conducted in a greenhouse on the Maringá-PR campus of the State University of Maringá. The design was a randomized complete block design in a 6x4 factorial scheme with four replications. The factor A comprised six nitrogen sources: Control, strain UFLA 02 100, strain CIAT 899, Nitrogen 60 kg ha-1, strain UFLA 02 100 + Nitrogen 30 kg ha-1, strain CIAT 899 + Nitrogen 30 kg ha-1 and the B factor is the phenological stages V4, R5, R6 and R8. The analyzed variables were: dry mass of nodules (DMN, grams/plant), dry mass of shoot (DMS, grams/plant), nitrogen content in shoot (NCS, %) and accumulation of nitrogen in shoot (ANS, grams/plant). For the statistical analysis of the data, the means test was used for the variables in the stages. The CIAT 899 strain statistically outperformed UFLA 02 100 for most variables and it was found that nitrogen fertilization negatively affected nodulation.  


Genetika ◽  
2012 ◽  
Vol 44 (2) ◽  
pp. 279-284 ◽  
Author(s):  
Reza Golparvar

In order to determine the best indirect selection criteria for genetic improvement of biological nitrogen fixation, sixty four common bean genotypes were cultivated in two randomized complete block design. Genotypes were inoculated with bacteria Rhizobium legominosarum biovar Phaseoli isolate L-109 only in one of the experiments. The second experiment was considered as check for the first. Correlation analysis showed positive and highly significant correlation of majority of the traits with percent of nitrogen fixation. Step-wise regression designated that traits percent of total nitrogen of shoot, number of nodule per plant and biological yield accounted for 92.3 percent of variation exist in percent of nitrogen fixation. Path analysis indicated that these traits have direct and positive effect on percent of nitrogen fixation. Hence, these traits are promising indirect selection criteria for genetic improvement of nitrogen fixation capability in common bean genotypes especially in early generations.


Author(s):  
Edna Maria Bonfim-Silva ◽  
Maria Débora Loiola Bezerra ◽  
Tonny José Araújo da Silva ◽  
William Fenner ◽  
Ana Paula Alves Barreto Damasceno

This study evaluated the production of Paiaguás grass (Urochloa brizantha) as a function of wood-ash doses and water availability in the Cerrado Oxisol. The experiment was carried out in a greenhouse with a randomized block design in a fractioned 5x5 factorial scheme, corresponding to five wood-ash doses (0; 8; 16; 24 and 32 g dm-3) and five soil water-availability scenarios (4; 8; 16; 32; 64 kPa) with four repetitions. The experimental design was based on the modified central compound and consisted of 13 treatments of wood-ash doses (g dm-3) and soil water-availability scenarios (kPa): 0-4; 0-16; 0-64; 8-8; 8-32; 16-4; 16-16; 16-64; 24-8; 24-32; 32-4; 32-16; 32-64. Soil volumetric moisture was monitored daily by means of the Diviner 2000 Capacitance Probe® for soil water replenishment according to the treatments. In 30-day intervals, three cuts were performed in the aerial part of the plants evaluating dry mass of leaves, stems, aerial part, number of leaves and of tillers. Data were subjected to analysis of variance and when significant to regression analysis, both up to 5% of error probability, through the statistical program SISVAR and response surface study using SAS. The combinations of wood ash doses with water soil tensions influence the productive characteristics of Paiaguás grass. Fertilization with wood ash reduces the effect of water stress on Paiaguás grass and this residue may be a viable alternative to partial replacement of mineral fertilization and safe disposal in the environment.


FLORESTA ◽  
2020 ◽  
Vol 50 (2) ◽  
pp. 1231
Author(s):  
Rodrigo Camara ◽  
Cristiane Figueira da Silva ◽  
Marcos Gervasio Pereira ◽  
Gabriela Cavalcanti Alves ◽  
Dereck Halley Anthony Alves Ferreira ◽  
...  

Mineral fertilization is an important practice that contributes to the production of seedlings with satisfactory quality. This study aimed to evaluate the effect of the application of potassium chloride (KCl) and monoammonium phosphate (MAP) on growth and nutrition of Eucalyptus urophylla x Eucalyptus grandis seedlings. The experiment was conducted in full sun, with direct sowing in tubes containing composite substrate (Pinus bark, coconut fiber, bovine manure and vermiculite). The experimental design was a randomized block design in a 5 x 2 factorial scheme, with five doses of KCl (0.0, 0.025, 0.050, 0.100, and 0.200 g seedling-1) and two doses of MAP (0 and 0.10g seedling-1), with four replicates. At 120 days, the effect of the interaction between the KCl and MAP doses was verified for the variables: diameter at root collar, dry mass of the aerial part (DMAP) and total (TDM), Dickson Quality Index, N content, P K in the aerial part, K content in the roots, absorption efficiency (AE) of N and P. For the variables: height (H); leaf area; root dry mass (RDM); H/DRC and DMPA/RDM ratios; and N and P content in the roots, only effect of the application of MAP is observed, whereas for AE_K the effect was only of KCl. Thus, there is variation in the effect of KCl doses as a function of morphological and nutritional variables evaluated. In general, the best seedling growth and nutrition occurs with the applied dose of 0.100 g KCl + 0.10 g MAP seedling -1.


2021 ◽  
Vol 34 (2) ◽  
pp. 359-369
Author(s):  
AMANDA CORDEIRO DE MELO SOUZA ◽  
THIAGO PONTES LIRA ◽  
ANTONIO FÉLIX DA COSTA ◽  
FELIPE JOSÉ CURY FRACETTO ◽  
GISELLE GOMES MONTEIRO FRACETTO ◽  
...  

ABSTRACT Cowpeas (Vigna unguiculata L. Walp) are an economically and socially important legume in northern and north-eastern Brazil and can establish effective symbiosis with nitrogen-fixing bacteria. We evaluated the symbiotic compatibility and efficiency of rhizobial strains from Pernambuco semi-arid soils and determined their symbiotic stability on the IPA-206, BR 17-Gurguéia, and BRS Novaera cultivars, selected for different environments. The experiment was conducted in a greenhouse to evaluate a 3 × 28 factorial arrangement (cultivars selected for different environments × inoculation with the currently recommended strain, uninoculated plants with or without mineral nitrogen, and 25 rhizobial strains from semi-arid soils) in a randomized block design with four replicates. We determined nodule number, shoot and root dry matter, nodule dry matter by nodule number, nitrogen accumulated in the shoot by nodule dry matter, nitrogen content and accumulation in the shoot, relative efficiency of the recommended strain based on nitrogen accumulation, and shoot dry matter. Overall, the cultivars responded differently to different strains and cultivar biological nitrogen fixation potential. Strains G7.85 and BR 3262 showed potential for biological nitrogen fixation. BR 3262 was confirmed to be adequate for inoculation of different cowpea cultivars.


2005 ◽  
Vol 62 (2) ◽  
pp. 138-144 ◽  
Author(s):  
Marta dos Santos Freire Ricci ◽  
Bruno José Rodrigues Alves ◽  
Simone Cordeiro de Miranda ◽  
Fabio Freire de Oliveira

In view of the low N concentration in organic fertilizers, it is necessary to use high rates of such fertilizers to attend coffee crop requirements. Hence, N is the most limiting nutrient for organic coffee production. The objective of this work was to evaluate the influence of sunn hemp (Crotalaria juncea) organic fertilization on the growth and nutritional status of coffee cultivars, as well as to quantify plant biomass and N input derived from biological nitrogen fixation, and their effect on soil chemical characteristics. The experiment consisted of six coffee (Coffea arabica) cultivars intercropped with and without sunn hemp sown in November 2001 and pruned at mid-height 76 days later. At 175 days, the standing biomass of the legume was cut, measuring dry mass, total N, P, K, Ca, Mg, and 15N natural abundance, resulting 16 t ha-1 of dry mass and the recycling of 444, 21, 241, 191, and 44 kg ha-1 of N, P, K, Ca, and Mg, respectively. Cultivars 'Obatã' and 'Catuaí Vermelho' presented the highest growth rates in terms of plant height, while cultivars 'Icatu' and 'Oeiras' presented the lowest rates. Biological nitrogen fixation associated to the legume introduced more than 200 kg ha-1 of N, which is a demonstration that N fertilization in organic cropping systems is a valuable alternative. Intercropping lead to a constant coffee leaf N content during the entire cropping cycle, contrary to what was observed in plots grown without sunn hemp.


2019 ◽  
Vol 8 (1) ◽  
pp. 82
Author(s):  
Simunji Simunji ◽  
Kalaluka L. Munyinda ◽  
Obed I. Lungu ◽  
Alice M. Mweetwa ◽  
Elijah Phiri

Nitrogen is a major plant nutrient which is most limiting in the soil due to soil losses of mineral nitrogen (N) form. To ensure availability of nitrogen in the soil, the study was conducted to screen four cowpea genotypes for Biological Nitrogen Fixation (BNF) and their contribution to maize yield in maize- cowpea rotation. The cowpea genotypes used were mutants LT11-3-3-12 (LT) and BB14-16-2-2 (BB) and their parental varieties Lutembwe (LTPRT) and Bubebe (BBPRT) respectively. Trials were established at two sites (Chisamba and Batoka) of different soil types. The Randomized Complete Block Design (RCBD) with three replications was used. Labelled 15N urea was applied at 20kgNha-1 on the four cowpea genotypes during 2015/16 growing season. Cowpea plant parts were dried and milled for 15N isotopic analysis. The data collected included Nitrogen content and atom % 15N excess in the fixing cowpea genotypes and non-nitrogen fixing pearl millet to determine total nitrogen derived from the atmosphere (TNdfa) and total nitrogen (TN) in plant parts which were further used to compute Biological Nitrogen Fixation (BNF). The results showed that BNF by cowpea genotypes at Chisamba was 63.9 kg ha-1 and was significantly (P<0.001) more than BNF of 6.6 kgha-1 at Batoka. The LT mutant fixed significantly (P<0.001) higher nitrogen of 86.1 kgha-1 and 16.5kg ha-1 at Chisamba and Batoka respectively than other genotypes. However, both BB and LT mutants significantly fixed more nitrogen than their parents and have demonstrated to increase maize grain yields up-to 12 tha-1 in the maize – cowpea rotation.


2021 ◽  
Vol 6 ◽  
pp. 1-9
Author(s):  
Roberto Takane ◽  
Cyro Fragoso Silva ◽  
Jessica Soares Pereira ◽  
Christian Morimasa Takemura ◽  
Talita Angélica ◽  
...  

The present work aimed to evaluate the influence of use, as well as to determine adequate doses in organic fertilization of the bokashi type in the cultivation of two basil (Ocimum basilicum L.) cultivars. Seeds from the collection of the Floriculture Laboratory of the Phytotechnics Department of the Federal University of Ceará were used. The treatments used were arranged in a randomized block design (RBD), arranged in a 2x5 factorial scheme, consisting of two cultivars of basil (Genovese and Anão) and five doses of Bokashi, namely: 0, 3, 6, 9 and 18 g.L-1. Plants that received no dose (0 g.L-1) served as a control treatment. Each treatment contained 5 repetitions, with the plot consisting of 7 pots, one plant per pot, the useful unit a pot with a basil seedling. From the transplant, applications of bokashi were started in doses of 0, 3, 6, 9, and 18 g.L-1 in both cultivars. Both applications were performed every 20 days after the first application of Bokashi. Fivety five days after transplantation in the final pot, the following variables were analyzed: plant height, stem diameter, fresh and dry mass of the aerial part and the root. The 9 g.L-1 dose of Bokashi showed the best results for the variables analyzed for both cultivars, in addition to being efficient when compared to the control, in which the vegetative growth was incipient.


Author(s):  
Welcio R. da Silva ◽  
Leandro C. Salomão ◽  
Débora R. M. Pereira ◽  
Henrique F. E. de Oliveira ◽  
Alexandre I. de A. Pereira ◽  
...  

ABSTRACT One of the potential alternatives to improve the use of water resources is the use of hydro retainer polymer applied to the soil. The objective of this study was to evaluate how irrigation levels and doses of hydro retainer polymer interfere in the production of ’Vanda’ lettuce. The experiment was carried out from December 2017 to January 2018 under protected environment in the Instituto Federal Goiano, Urutaí Campus, Goiás State, GO, Brazil (17º 29’ 10” S of latitude, 48º 12’ 38” W of longitude and 697 m of altitude). The work tested four irrigation levels of 50, 75, 100 and 125% of the evaporation obtained daily by the hook micrometer and five doses of hydrogel polymer (0, 50, 100, 150 and 200 g plant-1). The experimental design was a randomized block design in a scheme of split-plot and four blocks. Canopy fresh and dry mass, plant height, number of leaves and productivity were evaluated. There was 29.24% increase in the dry mass comparing the hydro retainer polymer doses of 0 and 169.28 g and an increase of 14.36% of the height of plants when compared with the irrigation levels of 50 and 125%. The use of a hydro retainer polymer provided a significant increase in all the evaluated productive variables. The irrigation levels influence the fresh mass from aerial part, plant height and productivity. The productivity increased 18.42% for the irrigation levels used and the highest estimated values were presented in the dose of 157.49 g (14.46 t ha-1) hydro retainer polymer.


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