scholarly journals ß-glycosidase and peroxidase activity in yellow lupin seedling infected by Fusarium oxysporum f. sp. lupini

2012 ◽  
Vol 58 (2) ◽  
pp. 103-110 ◽  
Author(s):  
Magdalena Rybus-Zając ◽  
Iwona Morkunas
Keyword(s):  
Fusarium Oxysporum ◽  
Seedling Growth ◽  
Peroxidase Activity ◽  
Biochemical Composition ◽  
Yield Loss ◽  
Defense Mechanism ◽  
Pathogenic Fungi ◽  
Metabolic Changes ◽  
Yellow Lupin ◽  
Temporary Inhibition

Lupine diseases caused by pathogenic fungi constitute a serious problem in agriculture. They lead to partial yield loss and deterioration of crop quality through the changes in biochemical composition of seeds or their contamination with mycotoxins. Some of common lupine diseases are fusarioses caused by <i>Fusarium oxysporum</i> f. sp. <i>lupini</i>. Morphometric and metabolic changes were investigated in yellow lupine seedlings infected with <i>F. oxysporum</i> f. sp. <i>lupini</i>. It was found that infection caused temporary inhibition of seedling growth, overcome at later development, and activation of <i>ß</i>-glycosidase and peroxidases. The changes in enzymes activity indicate the induction of defense mechanism against <i>F. oxysporum</i> f. sp. <i>lupini</i> and inhibition of pathogen spread.

scholarly journals Analysis of structures, functions, and transgenicity of phytopeptides defensin and thionin: a review

2021 ◽  
Vol 10 (1) ◽  
Author(s):  
Sarfuddin Azmi ◽  
Mohd Kamil Hussain
Keyword(s):  
Transgenic Plants ◽  
Antimicrobial Peptides ◽  
Defense Mechanism ◽  
Functional Characterization ◽  
Harmful Effect ◽  
Intrinsic Property ◽  
Pathogenic Fungi ◽  
Biotic And Abiotic Stress ◽  
Good Efficiency ◽  
Innate Defense

Abstract Background Antimicrobial peptides are very primitive innate defense molecules of almost all organisms, from microbes to mammalians and vascular seed-bearing plants. Antimicrobial peptides of plants categorized into cysteine-rich peptides (CRPs) and others and most of the antimicrobial peptides belong to CRPs group. These peptides reported showing the great extent of protecting property against bacteria, fungi, viruses, insect, nematode, and another kind of microbes. To develop a resistant plant against pathogenic fungi, there have been several studies executed to understand the efficiency of transgenicity of these antimicrobial peptides. Main text Apart from the intrinsic property of the higher organism for identifying and activating microbial attack defense device, it also involves innate defense mechanism and molecules. In the current review article, apart from the structural and functional characterization of peptides defensin and thionin, we have attempted to provide a succinct overview of the transgenic development of these defense peptides, that are expressed in a constitutive and or over-expressive manner when biotic and abiotic stress inflicted. Transgenic of different peptides show different competence in plants. Most of the transgenic studies made for defensin and thionin revealed the effective transgenic capacity of these peptides. Conclusion There have been several studies reported successful development of transgenic plants based on peptides defensin and thionin and observed diverse level of resistance-conferring potency in different plants against phytopathogenic fungi. But due to long regulatory process, there has not been marketed any antimicrobial peptides based transgenic plants yet. However, success report state that possibly in near future transgenic plants of AMPs would be released with devoid of harmful effect, with good efficiency, reproducibility, stability, and least production cost.

scholarly journals Absorption and Elimination of the Allelochemical MBOA by Weeds during Seedling Growth

Agronomy ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 471
Author(s):  
Alberto Oliveros-Bastidas ◽  
José M. G. Molinillo ◽  
Francisco A. Macias ◽  
Nuria Chinchilla
Keyword(s):  
Growth Medium ◽  
Seedling Growth ◽  
Defense Mechanism ◽  
Root Exudation ◽  
Kinetic Studies ◽  
Degradation Process ◽  
Lolium Rigidum ◽  
Weed Species ◽  
External Threats ◽  
Xenobiotic Compound

6-Methoxy-2-benzoxazolinone (MBOA) is an allelochemical that is found in Poaceae and is generally associated with monocotyledon species. This compound is formed from the glycosylated form of 2,4-dihydroxy-(2H)-1,4-benzoxazin-3(4H)-one (Gly-DIMBOA) by a two-stage degradation process. The MBOA detoxification capacity of two weed species, namely Echinochloa crus-galli and Lolium rigidum, and a resistant biotype of Lolium rigidum (SLR31) was studied both qualitatively and quantitatively. The product of metabolism is similar for both weed species. This finding indicates that these weeds probably metabolize xenobiotics by an identical route, since the product detected was the same in both cases. Kinetic studies on the absorption and translocation to the shoot showed differences in these processes depending on the species. The analysis of treated plants, which were subsequently transplanted to a growth medium without xenobiotic compound, showed that the weeds studied are capable of transmitting the previously absorbed compound to the medium by root exudation. The results show that this process is another defense mechanism of plants facing external threats.

scholarly journals Bright Side of Fusarium oxysporum: Secondary Metabolites Bioactivities and Industrial Relevance in Biotechnology and Nanotechnology

2021 ◽  
Vol 7 (11) ◽  
pp. 943
Author(s):  
Sabrin R. M. Ibrahim ◽  
Alaa Sirwi ◽  
Basma G. Eid ◽  
Shaimaa G. A. Mohamed ◽  
Gamal A. Mohamed
Keyword(s):  
Fusarium Oxysporum ◽  
Cyclic Peptides ◽  
Pathogenic Fungi ◽  
New Drugs ◽  
Biotechnological Applications ◽  
The World ◽  
Different Types ◽  
Bright Side ◽  
Reported Data ◽  
Industrial Relevance

Fungi have been assured to be one of the wealthiest pools of bio-metabolites with remarkable potential for discovering new drugs. The pathogenic fungi, Fusarium oxysporum affects many valuable trees and crops all over the world, producing wilt. This fungus is a source of different enzymes that have variable industrial and biotechnological applications. Additionally, it is widely employed for the synthesis of different types of metal nanoparticles with various biotechnological, pharmaceutical, industrial, and medicinal applications. Moreover, it possesses a mysterious capacity to produce a wide array of metabolites with a broad spectrum of bioactivities such as alkaloids, jasmonates, anthranilates, cyclic peptides, cyclic depsipeptides, xanthones, quinones, and terpenoids. Therefore, this review will cover the previously reported data on F. oxysporum, especially its metabolites and their bioactivities, as well as industrial relevance in biotechnology and nanotechnology in the period from 1967 to 2021. In this work, 180 metabolites have been listed and 203 references have been cited.

scholarly journals Effect of Proline and Aspirin on Seed Germination and Seedling Growth of Lycopersicon esculentum and Surface Growth of Fusarium oxysporum

2014 ◽  
Vol 11 (2) ◽  
pp. 813-818
Author(s):  
Baghdad Science Journal
Keyword(s):  
Seed Germination ◽  
Lycopersicon Esculentum ◽  
Fusarium Oxysporum ◽  
Seedling Growth ◽  
Surface Growth ◽  
Seed Decay ◽  
Effect Of Surface ◽  
Infection Percentage

Research was conducted to study the effect of proline and aspirin with 10 and 20 ppm on seed germination and seedling growth of Lycopersicon esculentum and the effect of surface growth of Fusarium oxysporum. The results showed that the proline and aspirin effected significantly to decreased percentage of seed germination, acceleration of germination, promoter indicator, elongation speed of radical and plumule and also the infection percentage of seed decay and surface growth of Fusarium oxysporum was reduced significantly.

Screening of Chickpea Genotypes for Resistance against Fusarium Wilt

2019 ◽  
pp. 1-6 ◽  
Author(s):  
Sanjeev Kumar ◽  
Sangita Sahni ◽  
Birendra Kumar
Keyword(s):  
Fusarium Oxysporum ◽  
Cicer Arietinum ◽  
Fusarium Wilt ◽  
Yield Loss ◽  
Wilt Disease ◽  
Alternative Approach ◽  
Legume Crops ◽  
Chemical Pesticides

Chickpea (Cicer arietinum) is one of the world’s major legume crops and suffers substantial damage from wilt disease incited by Fusarium oxysporum f. sp. ciceri (Padwick) with yield loss over 60 per cent. The screening for new resistance chickpea genotypes against this disease is an alternative approach to avoid indiscriminate use of chemical pesticides. In this study 55 chickpea genotypes were screened against Fusarium wilt. Out of 55 chickpea genotypes studied, only one genotype was found to be resistant and 12 were found to be moderately resistance. Nineteen genotypes showed moderately susceptible. However, nineteen and four genotypes showed susceptible and highly susceptible reaction for wilt disease, respectively.

scholarly journals Defense Responses of Fusarium oxysporum to 2,4-Diacetylphloroglucinol, a Broad-Spectrum Antibiotic Produced by Pseudomonas fluorescens

2004 ◽  
Vol 17 (11) ◽  
pp. 1201-1211 ◽  
Author(s):  
Alexander Schouten ◽  
Grardy van den Berg ◽  
Véronique Edel-Hermann ◽  
Christian Steinberg ◽  
Nadine Gautheron ◽  
...  
Keyword(s):  
Fusarium Oxysporum ◽  
Pseudomonas Fluorescens ◽  
Broad Spectrum ◽  
Acid Production ◽  
Fusaric Acid ◽  
Geographic Origin ◽  
Intergenic Spacer ◽  
Pathogenic Fungi ◽  
Defense Responses ◽  
Broad Spectrum Antibiotic

A collection of 76 plant-pathogenic and 41 saprophytic Fusarium oxysporum strains was screened for sensitivity to 2,4-diacetylphloroglucinol (2,4-DAPG), a broad-spectrum antibiotic produced by multiple strains of antagonistic Pseudomonas fluorescens. Approximately 17% of the F. oxysporum strains were relatively tolerant to high 2,4-DAPG concentrations. Tolerance to 2,4-DAPG did not correlate with the geographic origin of the strains, formae speciales, intergenic spacer (IGS) group, or fusaric acid production levels. Biochemical analysis showed that 18 of 20 tolerant F. oxysporum strains were capable of metabolizing 2,4-DAPG. For two tolerant strains, analysis by mass spectrometry indicated that deacetylation of 2,4-DAPG to the less fungitoxic derivatives monoacetylphloroglucinol and phloroglucinol is among the initial mechanisms of 2,4-DAPG degradation. Production of fusaric acid, a known inhibitor of 2,4-DAPG biosynthesis in P. fluorescens, differed considerably among both 2,4-DAPG-sensitive and -tolerant F. oxysporum strains, indicating that fusaric acid production may be as important for 2,4-DAPG-sensitive as for -tolerant F. oxysporum strains. Whether 2,4-DAPG triggers fusaric acid production was studied for six F. oxysporum strains; 2,4-DAPG had no significant effect on fusaric acid production in four strains. In two strains, however, sublethal concentrations of 2,4-DAPG either enhanced or significantly decreased fusaric acid production. The implications of 2,4-DAPG degradation, the distribution of this trait within F. oxysporum and other plant-pathogenic fungi, and the consequences for the efficacy of biological control are discussed.

Ultrastructural study of galacturonic acid distribution in some pathogenic fungi using gold-complexed Aplysia depilans gonad lectin

1989 ◽  
Vol 35 (3) ◽  
pp. 349-358 ◽  
Author(s):  
Nicole Benhamou
Keyword(s):  
Candida Albicans ◽  
Plasma Membrane ◽  
Fusarium Oxysporum ◽  
Ultrastructural Study ◽  
Colloidal Gold ◽  
Cell Walls ◽  
Galacturonic Acid ◽  
Pathogenic Fungi ◽  
Ophiostoma Ulmi ◽  
Ascomycete Fungi

Aplysia gonad lectin, isolated from the mollusc Aplysia depilans, was successfully conjugated to colloidal gold and used for ultrastructural detection of galacturonic acids in some pathogenic fungi. These sugar residues were found to occur in the fibrillar sheath surrounding hyphal cells of Ascocalyx abietina and in intravacuolar dense inclusions of this fungus spores. In hyphae and spores of Ophiostoma ulmi, galacturonic acids were detected mainly in the outermost wall layers. In contrast, these saccharides appeared associated with the innermost wall layers and especially the plasma membrane of Verticillium albo-atrum cells. Galacturonic acids were found to be absent in cells of Fusarium oxysporum f.sp. radicis-lycopersici and Candida albicans. These cytochemical data indicate therefore that a heterogeneity in wall composition exists between ascomycete fungi. The significance of the presence of galacturonic acids in the cell walls of certain fungi is still open to question.Key words: galacturonic acid, fungi, gold labeling, Aplysia depilans gonad lectin.

scholarly journals Wheat pathogen Zymoseptoria tritici N-myristoyltransferase inhibitors: on-target antifungal activity and an unusual metabolic defense mechanism

2020 ◽  
Author(s):  
Roman O. Fedoryshchak ◽  
Cory A. Ocasio ◽  
Ben Strutton ◽  
Jo Mattocks ◽  
Andrew J. Corran ◽  
...  
Keyword(s):  
Defense Mechanism ◽  
Acquired Resistance ◽  
Unmet Need ◽  
Pathogenic Fungi ◽  
Time Profile ◽  
Septoria Tritici ◽  
Chemical Toxicity ◽  
Zymoseptoria Tritici ◽  
Proteomic Investigation

ABSTRACTZymoseptoria tritici is the causative agent of Septoria tritici blotch (STB), which costs billions of dollars annually to major wheat-producing countries in terms of both fungicide use and crop loss. Agricultural pathogenic fungi have acquired resistance to most commercially available fungicide classes, and the rate of discovery and development of new fungicides has stalled, demanding new approaches and insights. Here we investigate a potential mechanism of targeting an important wheat pathogen Z. tritici via inhibition of N-myristoyltransferase (NMT). We characterize Z. tritici NMT biochemically for the first time, profile the in vivo Z. tritici myristoylated proteome and identify and validate the first Z. tritici NMT inhibitors. Proteomic investigation of the downstream effects of NMT inhibition identified an unusual and novel mechanism of defense against chemical toxicity in Z. tritici through the application of comparative bioinformatics to deconvolute function from the previously largely unannotated Z. tritici proteome. Research into novel fungicidal modes-of-action is essential to satisfy an urgent unmet need for novel fungicide targets, and we anticipate that this study will serve as a useful proteomics and bioinformatics resource for researchers studying Z. tritici.

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