scholarly journals Analysis of Predictive Factors of Antibiotic Resistant Pseudomonas aeruginosa in Children with Acute Appendicitis

2021 ◽  
Vol In Press (In Press) ◽  
Author(s):  
Mengqi Wan ◽  
Mengjie Yu ◽  
Juhua Xiao Jiangxi ◽  
Qiang Chen ◽  
Nuoya Li ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antibiotic Resistance ◽  
Acute Appendicitis ◽  
Sensitivity And Specificity ◽  
Infected Group ◽  
Antibiotics Resistance ◽  
Control Group ◽  
Biochemical Indicators ◽  
Blood Biochemical ◽  
Blood Biochemical Indicators

Objectives: To analyze the antibiotic resistance in children with acute appendicitis caused by Pseudomonas aeruginosa(P. aeruginosa), and to explore the predictors of infection and antibiotic resistance in children with acute appendicitis through blood biochemical indicators Methods: 50 control children (with no bacteria detected in the culture) and 103 children with P. aeruginosa infection who were admitted to the general surgery department of Jiangxi children’s hospital and treated surgically from January 2015 to June 2019 were analyzed. Results: Cefotaxime, cotrimoxazole and tetracycline had the highest drug resistance rates in children with appendicitis caused by P. aeruginosa, with (93.20%, 96/103), (93.20%, 96/103) and (93.20%, 96/103), respectively. There were significant pattern differences between the P. aeruginosa infected group and penicillin antibiotics resistant group. The combination of glutamyltranspeptidase (GGT), phosphorus (P), free fatty acid (FFA) and fibrinogen (CFbg) could effectively identify whether children with acute appendicitis were infected by P. aeruginosa and whether they were resistant to penicillin antibiotics. For the control group and P. aeruginosa infected group, area under the receiver operating characteristic (AUROC) value of the four indicators was 0.9034 (95% CI, 0.8514 - 0.9555), with a sensitivity and specificity of 84.76% and 87.93%, respectively. For the group with or without penicillin antibiotics resistance, the AUROC value was 0.9341 (95% CI, 0.8681 - 1.0000), with a sensitivity and specificity of 89.04% and 96.88%, respectively. Conclusions: Clinical blood biochemical indicators can reflect bacterial infection and antibiotic resistance in children with acute appendicitis to a certain extent, and the combination of GGT, P, FFA, and CFbg can predict P. aeruginosa infection and penicillin antibiotics resistance in children with acute appendicitis.

Effect of pig housing system on blood biochemical indicators’ level

2018 ◽  
Vol 35 (4) ◽  
pp. 45-51
Author(s):  
Maria Tietze ◽  
Wanda Krupa ◽  
Monika Budzyńska ◽  
Jarosław Kamieniak ◽  
Leszek Sołtys ◽  
...  
Keyword(s):  
Housing System ◽  
Biochemical Indicators ◽  
Blood Biochemical ◽  
Blood Biochemical Indicators

scholarly journals Effect of Various Feed Phosphates on Biochemical Indices of Blood and Mineral Composition of Bones in Finishing Pigs

2010 ◽  
Vol 79 (3) ◽  
pp. 355-361 ◽  
Author(s):  
Zbigniew Dobrzański ◽  
Krystyna Pogoda-Sewerniak ◽  
Szymon Dragan ◽  
Daniel Korniewicz ◽  
Krystyna Hoffmann ◽  
...  
Keyword(s):  
Lactic Dehydrogenase ◽  
Low Density Lipoproteins ◽  
High Density Lipoproteins ◽  
Low Density ◽  
Finishing Pigs ◽  
Biochemical Indicators ◽  
Blood Biochemical ◽  
Mineral Components ◽  
Blood Biochemical Indicators ◽  
Biochemical Indices

The aim of this study was to evaluate the effect of three different chemical feed phosphates on the blood biochemical indicators and the content of main minerals of bones in finishing pigs. Over a period of 85 days of fattening, monocalcium (MCP, Finnish product), dicalcium (DCP, Polish product) and calcium-sodium (CSP, Russian product) phosphates were used in fattener feeding. The feeding was based on standard mixtures of starter, grower and finisher type. Dicalcium phosphate was produced according to the new, pro-ecological technology based on phosphoric acid. The content of Ca, Na, P, solubility of P in citric acid, and the concentration of undesirable substances (As, Cd, F, Hg and Pb) were determined in feed phosphates. At the end of the fattening period, blood was collected from 36 finishing pigs (12 from each group) and the following biochemical indicators were determined in the serum: enzymatic activity of aspartate aminotransferase (AST), alanine aminotransferase (ALT), γ-glutamyltransferase (GGT), creatine kinase (CK), lactic dehydrogenase (LDH), lactic acid (LA); the concentration of total protein, albumins, glucose, urea, creatinine, content of triglycerides, cholesterol and its high density lipoproteins (HDL) and low density lipoproteins (LDL) fractions, and mineral components concentration (Ca, Cl, Cu, Fe, K, Mg, Na, P, Zn). Basic macroelement content (Ca, Mg, P) was determined in the thigh bones from 30 pigs (10 from each group). Significant differences (p < 0.05) between groups were observed only in some biochemical indicators, i.e. CK, LDH and LA. The highest content of Ca, Mg and P was found in the bones of pigs fed mixtures supplemented with DCP which indicates improved bioavailability of main macroelements from that phosphate.

Genetic parameters for the prediction of abdominal fat traits using blood biochemical indicators in broilers

2017 ◽  
Vol 59 (1) ◽  
pp. 28-33 ◽  
Author(s):  
H. L. Zhang ◽  
Z. Q. Xu ◽  
L. L. Yang ◽  
Y. X. Wang ◽  
Y. M. Li ◽  
...  
Keyword(s):  
Genetic Parameters ◽  
Abdominal Fat ◽  
Biochemical Indicators ◽  
Blood Biochemical ◽  
Blood Biochemical Indicators

scholarly journals Comparison of the Metabolites of Water Polo Players before and after Competition by the Metabolomic Approach

2021 ◽  
Vol 2021 ◽  
pp. 1-11
Author(s):  
Jingjing Wang ◽  
Mohammed Abdella Kemal
Keyword(s):  
Ketone Bodies ◽  
Urine Ph ◽  
Blood Samples ◽  
Biochemical Indicators ◽  
Water Polo ◽  
Metabolic Characteristics ◽  
Blood Biochemical ◽  
Before And After ◽  
Blood Biochemical Indicators ◽  
Metabolomic Approach

Background. The metabolic characteristics of body fluids of excellent water polo players before and after competition have not been reported. The purpose of the study was to compare the metabolites in the urine of water polo players before and after competition by 1H-NMR-based metabolomic approach. Methods. Twenty-six young water polo players participated in the study voluntarily. The urine and blood samples of players were collected one week before competition (A), immediately after competition (B), and one week after competition (C). Metabolomic analysis was conducted on the urine samples. Urine routine items and biochemical indicators in blood samples were detected. Results. Metabolomic results showed that the contents of eleven metabolites including lactic acid, acetoacetate, and succinic acid in the urine of the subjects increased and four metabolites such as dimethylamine, choline, and glucose decreased at stage B. Most metabolites at stage C had basically returned to the levels at stage A. Pyruvate metabolism, pantothenate and CoA biosynthesis, synthesis, and degradation of ketone bodies were mainly involved in the above process. Urine conventional analysis results showed that the urine pH decreased dramatically and the levels of PRO and URO significantly increased at stage B, and the three indicators had similar values between stages A and C. The other indicators did not have obvious difference among the three stages. Analysis of blood biochemical indicators showed that the levels of LDH, BUN, CK, and AST significantly increased at stage B and did not show an obvious difference between stages A and C. The results are helpful for coaches to arrange the athletes’ diet reasonably and to conduct scientific training for athletes.

scholarly journals The relationship between urinary Alzheimer-associated neuronal thread protein and blood biochemical indicators in the general population

Aging ◽  
2020 ◽  
Vol 12 (15) ◽  
pp. 15260-15280
Author(s):  
Yuxia Li ◽  
Shaochen Guan ◽  
He Jin ◽  
Hongjun Liu ◽  
Meimei Kang ◽  
...  
Keyword(s):  
General Population ◽  
Biochemical Indicators ◽  
Blood Biochemical ◽  
Blood Biochemical Indicators ◽  
The Relationship

scholarly journals Systematic Safety Evaluation of Cold Plasma-Activated Liquid in Rabbits

2021 ◽  
Vol 9 ◽  
Author(s):  
Yujing Xu ◽  
Sansan Peng ◽  
Bing Li ◽  
Shuai Wang ◽  
Hao Zhang ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Body Weight ◽  
New Zealand ◽  
Safety Evaluation ◽  
Toxicity Tests ◽  
Biochemical Indicators ◽  
Blood Biochemical ◽  
Acute Toxicity Tests ◽  
Blood Biochemical Indicators ◽  
New Zealand Rabbits

Plasma-activated liquid (PAL) can effectively and selectively kill various types of cancer cells both in superficial and deeper tumors. As a promising novel approach to oncotherapy, the safety of PAL is essential in the clinic but has not been thoroughly assessed. In myeloma and blood tumors, the pathogenesis is in the bone marrow cavity. We have therefore evaluated the safety of PAL in New Zealand rabbits by intra-bone marrow injection, and provide a basis of further clinical research and application of PALs. In this study, both a plasma jet and plasma surface were used to treat saline solution, phosphate-buffered solution, and cell culture medium, to produce PAL. Then, oscillograms and optical emission spectra were evaluated to characterize the plasma discharge. Acute toxicity tests and safety evaluation studies were conducted by intra-bone marrow injection of PAL into New Zealand rabbits, while control rabbits received saline only. Body weight, vital organ coefficient, organ appearance, organ histopathology, blood cell and hemoglobin parameters, and blood biochemical indicators were tested on the 30th day after injection. We found that there was no mortality or loss of mobility throughout the experimental period. Acute toxicity tests showed that there were no PAL-related side effects in rabbits receiving the maximum dose of 700 μL PAL. PAL treatments did not affect body weight, organ coefficient, organ appearance, organ histopathology, or blood biochemical indicators. However, the percentage of lymphocytes decreased while the percentage of neutrophil granulocytes increased compared with the control group. In summary, our results indicate that PAL can be safely injected into bone marrow of New Zealand rabbits without significant toxicity.

Effects of Water Temperature on Biochemical Characteristics of Mandarin Fish during Live Transportation

2014 ◽  
Vol 941-944 ◽  
pp. 1092-1098
Author(s):  
Shou Kun Cheng ◽  
Shun Sheng Chen ◽  
Li Juan Wang ◽  
Shuo Lin Huang
Keyword(s):  
Respiratory Rate ◽  
Water Temperature ◽  
Short Distance ◽  
Ammonia Nitrogen ◽  
Mandarin Fish ◽  
Long Distance ◽  
Biochemical Indicators ◽  
Blood Biochemical ◽  
Opposite Trend ◽  
Blood Biochemical Indicators

Objective and Methods: The effect of water temperature on Mandarin fish during live transportation was studied through testing the respiratory rate, ammonia nitrogen in water, glycogen and lactic acid in flesh and blood biochemical indicators at 20°C, 15°C,10°C and 5°C after transported for 0h, 3h, 10h and 24h. Results: The respiratory rate declined with the decreasing temperature and extending transportation time; the ammonia nitrogen in water increased with the temperature falling and lapse of time; the glycogen dropped as time went by and 24h later it declined with the temperature falling; but lactic presented a completely opposite trend; The cortisol decreased with the temperature declined, and rose first and then fell with the time; AST had a negative correlation with water temperature but GLU had a positive correlation; UREA decreased significantly with the declined temperature between 20°C and 10°C, and the level of it at 5°C was close to at 20°C.Conclutions: All these results revealed that 5°C should be selected for short distance transportation less than 10h and 10-15°C was the best choice for long distance transportation.

Age-related Changes of Selected Blood Biochemical Indicators in Dairy Calves during Their First Week of Life

2011 ◽  
Vol 59 (1) ◽  
pp. 25-30 ◽  
Author(s):  
Agnieszka Herosimczyk ◽  
Adam Lepczyński ◽  
Alicja Dratwa-Chałupnik ◽  
Anna Kurpińska ◽  
Agnieszka Klonowska ◽  
...  
Keyword(s):  
Dairy Calves ◽  
Biochemical Indicators ◽  
Blood Biochemical ◽  
Age Related ◽  
Blood Biochemical Indicators ◽  
Age Related Changes

scholarly journals Correction of Poikylocytosis and Blood Biochemical Indicators in Acute Blood Loss

2015 ◽  
Vol 11 (3) ◽  
pp. 6
Author(s):  
V. V. Moroz ◽  
I. S. Novoderzhkina ◽  
E. M. Antoshina ◽  
A. V. Afanasyev ◽  
I. A. Ryzhkov ◽  
...  
Keyword(s):  
Blood Loss ◽  
Acute Blood Loss ◽  
Biochemical Indicators ◽  
Blood Biochemical ◽  
Blood Biochemical Indicators

scholarly journals Redox protein OsaR (PA0056) regulates dsbM and the oxidative stress response in Pseudomonas aeruginosa

2020 ◽  
Author(s):  
Yujie Liu ◽  
Yibing Ma ◽  
Zhongqiang Ma ◽  
Xiao Han ◽  
Hang Qi ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Pseudomonas Aeruginosa ◽  
Antibiotic Resistance ◽  
Stress Response ◽  
Gene Cluster ◽  
Oxidative Stress Response ◽  
Antibiotics Resistance ◽  
Transcriptional Level ◽  
Beta Lactam ◽  
Beta Lactam Antibiotics

Bacteria have evolved distinct molecular mechanisms as a defense against oxidative stress. The foremost regulator of oxidative stress response has been found to be OxyR. However, the molecular details of regulation upstream of OxyR remain largely unknown and need further investigation. Here, we characterize a oxidant stress and antibiotic tolerance regulator, OsaR (PA0056), produced by Pseudomonas aeruginosa. Mutation of osaR increased bacterial tolerance to aminoglycoside and beta-lactam antibiotics, as well as to hydrogen peroxide. Expression of the oxyR regulon genes oxyR, katAB, and ahpBCF was increased in the osaR mutant. However, the OsaR protein does not regulate the oxyR regulon genes through direct binding to their promoters. PA0055, osaR, PA0057 and dsbM are in the same gene cluster, and we provide evidence that expression of these genes involved in oxidant tolerance is controlled by binding of OsaR to intergenic region between osaR and PA0057, which contain two divergent promoters. The gene cluster is also regulated by PA0055 via an indirect effect. We further discovered that OsaR formed intramolecular disulfide bonds when exposed to oxidative stress, resulting in a change of its DNA binding affinity. Taken together, our results indicate that OsaR is inactivated by oxidative stress and plays a role in the tolerance of P. aeruginosa to aminoglycoside and beta-lactam antibiotics. IMPORTANCE As opportunistic pathogen, Pseudomonas aeruginosa can cause serious infections which are hard to eradicate because of antibiotic resistance in immunodeficient patients. We found that OsaR is involved in oxidative stress and antibiotics resistance by regulation of downstream genes via redox state change. Research on factors affecting the transcriptional level of oxyR is very limited, but important since it has implications on antibiotic resistance. In this study, it was found that OsaR can indirectly inhibit transcription of oxyR. In addition the gene cluster composed of PA0055, osaR, PA0057 and dsbM was identified, and the associated regulatory mechanisms and functions were elucidated. Our work not only provides a mechanistic understanding of antibiotic tolerance regulation in P. aeruginosa, but also has significant implications for redox regulation in human pathogens in general.

Sign in / Sign up

Export Citation Format

Share Document