scholarly journals Characterisation and antimicrobial activity of silver nanoparticles derived from Vascellum pratense polysaccharide extract and sodium citrate

2018 ◽  
Vol 10 (1) ◽  
Author(s):  
Predrag Petrović ◽  
Danijela Kostić ◽  
Anita Klaus ◽  
Jovana Vunduk ◽  
Miomir Nikšić ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Sodium Citrate ◽  
Colloidal Solution ◽  
Biological Activities ◽  
Short Term ◽  
Absolute Value ◽  
Stabilizing Agents ◽  
Skin Conditions ◽  
Fungal Polysaccharides

Silver nanoparticles (AgNPs) were synthesized by “green”, cheap hydrothermal method in an autoclave using sodium citrate and Vascellum pratense polysaccharide extract as reducing and stabilizing agents. Presence of spherical AgNPs was confirmed by UV-VIS spectrophotometry and scanning electron microscopy; particle size was determined as ~ 40 nm. Even though colloidal solution had relatively low absolute value of zeta potential(-15 mV), short term stability studies suggested a stable system, with AgNPs being stabilized by both citrate and fungal polysaccharides, as FTIR spectra confirmed. The colloidal solution showed good antimicrobial activity against both G+/G- bacteria and Candida albicans, including methicilin resistant Staphylococcus aureus (MRSA). Products containing AgNPs and fungal polysaccharides, which possess various biological activities - most important being immunostimulation - may find use in treatment of skin conditions caused by pathogens.

scholarly journals Green Synthesis of Hydroxyethyl Cellulose-Stabilized Silver Nanoparticles

2013 ◽  
Vol 2013 ◽  
pp. 1-11 ◽  
Author(s):  
M. A. El-Sheikh ◽  
S. M. El-Rafie ◽  
E. S. Abdel-Halim ◽  
M. H. El-Rafie
Keyword(s):  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Colloidal Solution ◽  
Hydroxyethyl Cellulose ◽  
Synthesis Process ◽  
Reaction Conditions ◽  
Oh Groups ◽  
Stabilizing Agents ◽  
Transmission Electron ◽  
Optimum Reaction

Green synthesis aims to minimize the use of unsafe reactants and maximize the efficiency of synthesis process. These could be achieved by using environmentally compassionate polymers and nontoxic chemicals. Hydroxyethyl cellulose (HEC), an ecofriendly polymer, was used as both reducing and stabilizing agents in the synthesis of stable silver nanoparticles, while silver nitrate was used as a precursor and water as a solvent. The formation of silver nanoparticles was assessed by monitoring UV-vis spectra of the silver colloidal solution. The size of the nanoparticles was measured using transmission electron microscope (TEM). Reaction kinetics was followed by measuring the absorbance of silver colloidal solution at different time intervals. Optimum reaction conditions revealed that the highest absorbance was obtained using HEC : AgNO3 of 1.5 : 0.17 (g/100 cm3) at 70°C for 120 min at pH 12. The Ag0 nanoparticles colloidal solution so obtained (1000 ppm) were found stable in aqueous solution over a period of six months at room temperature (°C). The sizes of these nanoparticles were found in the range of 11–60 nm after six months of storing. FTIR spectra confirmed the interaction of both the aldehyde and OH groups in the synthesis and stabilization of silver nanoparticles.

scholarly journals A COMPARATIVE STUDY OF ANTIMICROBIAL ACTIVITY OF SILVER CLUSTERS AGAINST VARIOUS MICROORGANISMS

2016 ◽  
pp. 85-91 ◽  
Author(s):  
Tatiana Rozalyonok ◽  
Tatiana Rozalyonok ◽  
Yurii Sidorin ◽  
Yurii Sidorin
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Bacillus Subtilis ◽  
Candida Albicans ◽  
Aspergillus Niger ◽  
Antimicrobial Agents ◽  
Colloidal Solution ◽  
Cultured Cells ◽  
Silver Clusters

Currently, the problem of micro-organisms resistance to traditional antibiotics, which represents a serious threat to human health, is exposed to close attention. Therefore, the development of alternative antimicrobial agents, including on the basis of silver nanoparticles today becomes relevant. Substantiation of effectiveness of the cluster silver (1-10 nm) in comparison with larger silver nanoparticles is resulted. During the study of domestic and foreign experience of using a cluster of silver, basic mechanisms of its antimicrobial action were analyzed that it may have on organisms. The purpose was to comparatively study the antimicrobial activity of a cluster silver with respect to various microorganisms, including establishment of the minimum inhibitory silver concentration for the following strains: Escherichia coli , Bacillus subtilis , Candida albicans , Aspergillus niger . The effect of various concentrations of silver clusters (from 0 to 400 ug / ml) contained in a liquid medium, on survival of cultured cells was studied. Using the method of serial dilutions, the difference in effects on silver clusters on growth and reproduction of the following microorganisms was established: bacteria (with a different structure of cell walls: gram-positive-thick-walled, capable to form endospores and gram-negative - thin- walled) and micromycetes (yeast and hyphal). An in vitro study of antimicrobial activity of the cluster silver colloidal solution taken at various concentrations and at various exposure times was carried out. Minimum inhibitory concentrations of cluster silver colloidal solution for studied bacteria were determined: opportunistic pathogenic bacterium ( Escherichia coli and Bacillus subtilis ) and micromycetes ( Candida albicans and Aspergillus niger ).

scholarly journals Biosynthesis and characterization of silver nanoparticles induced by fungal proteins and its application in different biological activities

2019 ◽  
Vol 17 (1) ◽  
Author(s):  
Abdelmageed M. Othman ◽  
Maysa A. Elsayed ◽  
Naser G. Al-Balakocy ◽  
Mohamed M. Hassan ◽  
Ali M. Elshafei
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Large Scale ◽  
Biological Activities ◽  
Spherical Shape ◽  
Inhibition Zone ◽  
Scale Production ◽  
E Coli ◽  
Biosynthesized Agnps

Abstract Background The present study aims to apply an efficient eco-friendly and inexpensive process for green synthesis of silver nanoparticles (AgNPs) through the mediation of fungal proteins from Aspergillus fumigatus DSM819, characterization, and its application as antimicrobial finishing agent in textile fabrics against some infectious microorganisms. Results Optimum conditions for AgNP biosynthesis could be achieved by means of using 60% (v/v) of cell-free filtrate (CFF) and 1.5 mM of AgNO3 at pH 10.0 after 90 min. The obtained AgNPs were of spherical shape with 90% of distribution below than 84.4 nm. The biosynthesized AgNPs exerted an antimicrobial activity against the studied pathogenic microorganisms (E. coli, B. mycoides, and C. albicans). In addition, IC50 values against in vitro tumor cell lines were found to be 31.1, 45.4, 40.9, and 33.5 μg/ml for HCT116, A549, MCF7, and PC3, respectively. Even with a very low concentration (0.25%), the treated PET/C fabrics by AgNPs exerted an antimicrobial activity against E. coli, B. mycoides, and C. albicans to give inhibition zone diameter of 15, 15, and 16 mm, respectively. Conclusions The green biosynthesis approach applied in this study is a non-toxic alternative to the traditional chemical and physical methods, and would be appropriate for biological large-scale production and prospective treatments. Graphical abstract

A Simple Synthesis of Silver Nanoparticles on Cellulose Paper for Antimicrobial Applications

2020 ◽  
Vol 990 ◽  
pp. 191-196
Author(s):  
Windri Handayani ◽  
Arie Listyarini ◽  
Yasman ◽  
Cuk Imawan
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Antimicrobial Properties ◽  
Industrial Applications ◽  
Reduction Temperature ◽  
Clear Zone ◽  
Stabilizing Agents ◽  
Cellulose Paper ◽  
Shape And Size

Silver nanoparticles are well known for their antimicrobial properties and have been widely used in medical and industrial applications to prevent the growth of microorganisms. The effectiveness of silver nanoparticles (AgNPs) as an antimicrobial material is influenced by their shape and size; the smaller the size, the more active the AgNPs. To prevent aggregation, the nanoparticles can be maintained by stabilizing agents. This study aims to synthesize silver nanoparticles in situ using cellulose paper and to evaluate their antimicrobial activity. For reduction of Ag+ ions were used propane-1,2-diol. The reduction was carried out at 60, 80, and 100o C to accelerate the reaction. The formation of silver nanoparticles can be seen from the change in paper color from white to yellow or brown. This result is confirmed by the occurrence of the absorption peak in the UV-Vis spectrum between 300-500 nm. The TEM image shows the size distribution of silver nanoparticles affected by their reduction temperature. Antimicrobial tests provide a clear zone from the bottom of the paper to the edge. The reaction temperature at 60o C showed the best antimicrobial activity with AgNPs averaging at 6 nm. This method can be applied to antimicrobial paper preparation with controlled size and distribution.

scholarly journals Antimicrobial Properties of Lepidium sativum L. Facilitated Silver Nanoparticles

Pharmaceutics ◽  
2021 ◽  
Vol 13 (9) ◽  
pp. 1352
Author(s):  
Samir Haj Bloukh ◽  
Zehra Edis ◽  
Hamid Abu Sara ◽  
Mustafa Ameen Alhamaidah
Keyword(s):  
Silver Nanoparticles ◽  
Wound Care ◽  
Biological Activities ◽  
Health Sector ◽  
Antimicrobial Properties ◽  
Multidrug Resistant ◽  
Lepidium Sativum ◽  
One Pot ◽  
Stabilizing Agents ◽  
Lepidium Sativum L

Antibiotic resistance toward commonly used medicinal drugs is a dangerously growing threat to our existence. Plants are naturally equipped with a spectrum of biomolecules and metabolites with important biological activities. These natural compounds constitute a treasure in the fight against multidrug-resistant microorganisms. The development of plant-based antimicrobials through green synthesis may deliver alternatives to common drugs. Lepidium sativum L. (LS) is widely available throughout the world as a fast-growing herb known as garden cress. LS seed oil is interesting due to its antimicrobial, antioxidant, and anti-inflammatory activities. Nanotechnology offers a plethora of applications in the health sector. Silver nanoparticles (AgNP) are used due to their antimicrobial properties. We combined LS and AgNP to prevent microbial resistance through plant-based synergistic mechanisms within the nanomaterial. AgNP were prepared by a facile one-pot synthesis through plant-biomolecules-induced reduction of silver nitrate via a green method. The phytochemicals in the aqueous LS extract act as reducing, capping, and stabilizing agents of AgNP. The composition of the LS-AgNP biohybrids was confirmed by analytical methods. Antimicrobial testing against 10 reference strains of pathogens exhibited excellent to intermediate antimicrobial activity. The bio-nanohybrid LS-AgNP has potential uses as a broad-spectrum microbicide, disinfectant, and wound care product.

Preparation of Silvered Colloidal Compositions for Nanocosmetic Drugs

2020 ◽  
Vol 22 (1) ◽  
pp. 11
Author(s):  
N.N. Mofa ◽  
A.O. Zhapekova ◽  
B.S. Sadykov ◽  
A.E. Bakkara ◽  
M.I. Tulepov ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Silicon Dioxide ◽  
Ultrasonic Treatment ◽  
Metal Particle ◽  
Colloidal Solution ◽  
Silver Particles ◽  
Colloidal Systems ◽  
Activated State ◽  
Electrolysis Method

The article presents the results of obtaining nanosilver aqueous suspensions by electrolysis method with variations in the quality and composition of water, the type, and quantity of modifying additives, as well as the creation of colloidal compositions consisting of silicon dioxide and nanosilver emulsion as a result of mechanochemical treatment of the system. As a modifier of silver nanoparticles, citric acid, glycerin, and ether-cellulose were used, which can form the thinnest layers on the surface of the metal particle, preventing particles from sticking together and precipitating them. It is shown that the use of modifiers in the preparation of a colloidal solution with silver particles and ultrasonic treatment of the system provide an increase and stabilization of the activated state of colloidal systems with silver nanoparticles and silicon dioxide. Obtained under the influence of ultrasonic treatment homogeneous and resistant to delamination soft gel systems containing silver and having antimicrobial activity are promising for the manufacture of drugs for cosmetic purposes.

scholarly journals Curcumin to Promote the Synthesis of Silver NPs and their Self-Assembly with a Thermoresponsive Polymer in Core-Shell Nanohybrids

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Albanelly Soto-Quintero ◽  
Nekane Guarrotxena ◽  
Olga García ◽  
Isabel Quijada-Garrido
Keyword(s):  
Silver Nanoparticles ◽  
Self Assembly ◽  
Sodium Citrate ◽  
Polymer Chains ◽  
Metallic Surfaces ◽  
Thermoresponsive Polymer ◽  
One Pot ◽  
Stabilizing Agents ◽  
Silver Nps ◽  
Reducing Properties

AbstractThis work presents a simple one-pot protocol to achieve core-doped shell nanohybrids comprising silver nanoparticles, curcumin and thermoresponsive polymeric shell taking advantage of the reducing properties of phenolic curcumin substance and its ability to decorate metallic surfaces. Silver nanoparticles were synthesized, via sodium citrate and silver nitrate addition into a boiling aqueous solution of curcumin, monomers and surfactant. Curcumin and sodium citrate promoted silver nucleation, acting as reducing and stabilizing agents. These curcumin-capped AgNPs enabled, after adding the radical polymerization initiator, the assembling of the growing polymer chains around the hydrophobic AgNP surface. The resultant core-doped shell nanohybrids exhibit plasmonic, luminescent and volume thermoresponsive properties, with improved possibilities to be used as successful therapeutic platforms. In fact, the possibility to nanoconfine the synergistic antioxidant, antiviral, antibacterial features of silver and curcumin in one bioavailable hybrid paves the way to promising applications in the biomedical field.

Green Synthesis of Silver Nanoparticles by Allamanda cathartica L. Leaf Extract and Evaluation for Antimicrobial Activity

2013 ◽  
Vol 6 (4) ◽  
pp. 2260-2268
Author(s):  
M. Linga Rao ◽  
Bhumi G ◽  
Savithramma N
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Leaf Extract ◽  
Fusarium Species ◽  
Inhibition Zone ◽  
Fungal Species ◽  
Green Technology ◽  
Metallic Silver ◽  
Synthesis Of Nanoparticles ◽  
Aqueous Leaf Extract

Silver nanoparticles (SNPs) exhibit tremendous applications in medicine as antimicrobial agent.  The use of different parts of plants for the synthesis of nanoparticles is considered as a green technology as it does not involve any harmful chemicals.  In the present study, we report a rapid biosynthesis of silver nanoparticles from aqueous leaf extract of medicinal plant Allamanda cathartica.  The active phytochemicals present in the plant were responsible for the quick reduction of silver ion to metallic silver nanoparticles. The reduced silver nanoparticles were characterized by using UV-Vis spectrophotometry, Scanning Electron Microscope (SEM), Energy Dispersive Analysis of X-ray (EDAX) and Atomic Force Microscopy (AFM).  The spherical shaped silver nanoparticles were observed and it was found to 19-40 nm range of size.  These phytosynthesized SNPs were tested for their antimicrobial activity and it analyzed by measuring the inhibitory zone. A. cathartica aqueous leaf extract of SNPs showed highest toxicity to Pseudomonas followed by Klebsiella, Bacillus and E. coli and lowest toxicity towards Proteus. In fungal species, highest inhibition zone was noted against Rhizopus followed by Curvularia, Aspergillus flavus and Aspergillus niger and minimum inhibition zone was observed against Fusarium species.  These results suggest a promising potential of Indian plant-based green chemistry for production of SNPs for biomedical and nanotechnology applications.

Antimicrobial Activity of Silver Nanoparticles Prepared Under an Ultrasonic Field

2011 ◽  
Vol 4 (3) ◽  
pp. 1491-1496
Author(s):  
Umadevi M ◽  
Rani T ◽  
Balakrishnan T ◽  
Ramanibai R
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Reduction Method ◽  
Therapeutic Potential ◽  
Chemical Reduction ◽  
Ultrasonic Field ◽  
Great Promise ◽  
Chemical Reduction Method ◽  
E Coli

Nanotechnology has great promise for improving the therapeutic potential of medicinal molecules and related agents. In this study, silver nanoparticles of different sizes were synthesized in an ultrasonic field using the chemical reduction method with sodium borohydride as a reducing agent. The size effect of silver nanoparticles on antimicrobial activity were tested against the microorganisms Staphylococcus aureus (MTCC No. 96), Bacillus subtilis (MTCC No. 441), Streptococcus mutans (MTCC No. 497), Escherichia coli (MTCC No. 739) and Pseudomonas aeruginosa (MTCC No. 1934). The results shows that B. subtilis, and E. coli were more sensitive to silver nanoparticles and its size, indicating the superior antimicrobial efficacy of silver nanoparticles. 

Antimicrobial Activity of Silver Nanoparticles Synthesized by Streptomyces Species JF714876

2012 ◽  
Vol 5 (1) ◽  
pp. 1638-1642 ◽  
Author(s):  
Vidyasagar G M ◽  
Shankaravva B ◽  
R Begum ◽  
Imrose ◽  
Sagar R ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Silver Ions ◽  
Human Beings ◽  
Streptomyces Species ◽  
Force Microscopy ◽  
E Coli ◽  
Extracellular Synthesis ◽  
Atomic Force ◽  
Uv Visible

Microorganisms like fungi, actinomycetes and bacteria are considered nanofactories and are helpful in the production of nanoparticles useful in the welfare of human beings. In the present study, we investigated the production of silver nanoparticles from Streptomyces species JF714876. Extracellular synthesis of silver nanoparticles by Streptomyces species was carried out using two different media. Silver nanoparticles were examined using UV-visible, IR and atomic force microscopy. The size of silver nanoparticles was in the range of 80-100 nm. Antimicrobial activity of silver nanoparticle against bacteria such as E. coli, S. aureus, and dermatophytes like T. rubrum and T. tonsurans was determined. Thus, this study suggests that the Streptomyces sp. JF741876 can produce silver ions that can be used as an antimicrobial substance.

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