scholarly journals A roadmap for gene functional characterisation in wheat

2018 ◽  
Author(s):  
Nikolai M Adamski ◽  
Philippa Borrill ◽  
Jemima Brinton ◽  
Sophie Harrington ◽  
Clemence Marchal ◽  
...  
Keyword(s):  
Crop Improvement ◽  
Functional Genomic ◽  
Biological Processes ◽  
Model Species ◽  
High Quality ◽  
Genomic Resources ◽  
Current Review ◽  
Functional Characterisation ◽  
Recent Developments

Research in Arabidopsis and other model species has uncovered mechanisms regulating important biological processes in plants. With the advent of high quality functional genomic resources in wheat it is now possible to use this knowledge for crop improvement directly in wheat. In the current review, we describe some of the recent developments in wheat genomics focussing on published and publicly available resources and tools. We lay out a roadmap on how to make use of them and include a case study to exemplify them. We hope this review will be a helpful guide for plant scientists who already work on wheat or who are considering expanding their research into wheat.

scholarly journals Bioconversion of biomass: a case study of ligno-cellulosics bioconversions in solid state fermentation

1998 ◽  
Vol 41 (4) ◽  
pp. 379-390 ◽  
Author(s):  
Ashok Pandey ◽  
Carlos R. Soccol
Keyword(s):  
Solid State ◽  
Sugarcane Bagasse ◽  
Solid State Fermentation ◽  
Value Added ◽  
Biological Processes ◽  
Lignocellulosic Residues ◽  
Recent Developments ◽  
Feed Enzymes ◽  
Value Added Products

Lignocellulosic residues obtained from crops cultivation form useful sources to be used as substrate for bioconversion processes. Sugarcane bagasse, which is a complex substrate obtained from the processing of sugar cane, is an important biomass among such sources. Due to its abundant availability, it can serve as an ideal substrate for microbial processes for the production of value added products. This paper reviews recent developments on biological processes developed on production of various products in solid state fermentation using sugarcane bagasse as the substrate and describes production of protein enriched feed, enzymes, amino acid, organic acids and compounds of pharmaceutical importance, etc. through microbial means.

scholarly journals A roadmap for gene functional characterisation in crops with large genomes: Lessons from polyploid wheat

eLife ◽  
2020 ◽  
Vol 9 ◽  
Author(s):  
Nikolai M Adamski ◽  
Philippa Borrill ◽  
Jemima Brinton ◽  
Sophie A Harrington ◽  
Clémence Marchal ◽  
...  
Keyword(s):  
Gene Networks ◽  
Crop Improvement ◽  
Natural Populations ◽  
Basic Research ◽  
Nutrition Security ◽  
Functional Characterisation ◽  
Food And Nutrition ◽  
Crop Research ◽  
Made In

Understanding the function of genes within staple crops will accelerate crop improvement by allowing targeted breeding approaches. Despite their importance, a lack of genomic information and resources has hindered the functional characterisation of genes in major crops. The recent release of high-quality reference sequences for these crops underpins a suite of genetic and genomic resources that support basic research and breeding. For wheat, these include gene model annotations, expression atlases and gene networks that provide information about putative function. Sequenced mutant populations, improved transformation protocols and structured natural populations provide rapid methods to study gene function directly. We highlight a case study exemplifying how to integrate these resources. This review provides a helpful guide for plant scientists, especially those expanding into crop research, to capitalise on the discoveries made in Arabidopsis and other plants. This will accelerate the improvement of crops of vital importance for food and nutrition security.

Abiotic Stress Tolerance in Soybean : Regulated by ncRNA

2016 ◽  
Vol 3 (1) ◽  
Author(s):  
YASIN JESHIMA KHAN ◽  
HUSNARA Tyagi ◽  
Anil kumar Singh ◽  
Santosh kumar. Magadum
Keyword(s):  
Abiotic Stresses ◽  
Target Genes ◽  
Abiotic Stress Tolerance ◽  
Crop Improvement ◽  
Vital Role ◽  
Grain Legume ◽  
Biological Processes ◽  
Small Interfering Rnas ◽  
Cellular Environment ◽  
Non Coding Rnas

Plants respond through a cascade of reactions resulting in varied cellular environment leading to alterations in the patterns of protein expression resulting in phonotypic changes. Single cell genomics and global proteomics came out to be powerful tools and efficient techniques in studying stress tolerant plants. Non-coding RNAs are a distinct class of regulatory RNAs in plants and animals that control a variety of biological processes. Small ncRNAs play a vital role in post transcriptional gene regulation by either translational repression or by inducing mRNA cleavage. The major classes of small RNAs include microRNAs (miRNAs) and small interfering RNAs (siRNAs), which differ in their biogenesis. miRNAs control the expression of cognate target genes by binding to complementary sequences, resulting in cleavage or translational inhibition of the target RNAs. siRNAs too have a similar structure, function, and biogenesis like miRNAs but are derived from long double-stranded RNAs and can often direct DNA methylation at target sequences.In this review, we focus on the involvement of ncRNAs in comabting abiotic stresses of soybean. This review emphasis on previously known miRNAs as they play important role in several abiotic stresses like drought, salinity, chilling and heat stress by their diverse roles in mediating biological processes like gene expression, chromatin formation, defense of genome against invading viruses. This review attempts to elucidate the various kinds of non-coding RNAs explored, their discovery, biogenesis, functions, and response for different type of abiotic stresses and future aspects for crop improvement in the context of soybean, a representative grain legume.

scholarly journals Using the Google™ Search Engine for Health Information: Is There a Problem? Case Study: Supplements for Cancer

2021 ◽  
Vol 5 (2) ◽  
Author(s):  
Hannah C Cai ◽  
Leanne E King ◽  
Johanna T Dwyer
Keyword(s):  
Health Information ◽  
Search Engine ◽  
Information Quality ◽  
Nutrition Information ◽  
High Quality ◽  
Search Results ◽  
Health And Nutrition ◽  
Quality Rating

ABSTRACT We assessed the quality of online health and nutrition information using a Google™ search on “supplements for cancer”. Search results were scored using the Health Information Quality Index (HIQI), a quality-rating tool consisting of 12 objective criteria related to website domain, lack of commercial aspects, and authoritative nature of the health and nutrition information provided. Possible scores ranged from 0 (lowest) to 12 (“perfect” or highest quality). After eliminating irrelevant results, the remaining 160 search results had median and mean scores of 8. One-quarter of the results were of high quality (score of 10–12). There was no correlation between high-quality scores and early appearance in the sequence of search results, where results are presumably more visible. Also, 496 advertisements, over twice the number of search results, appeared. We conclude that the Google™ search engine may have shortcomings when used to obtain information on dietary supplements and cancer.

scholarly journals An Experimental Study on the Sources of Strontium in Mineral Water and General Rules of Its Dissolution—A Case Study of Chengde, Hebei

Water ◽  
2021 ◽  
Vol 13 (5) ◽  
pp. 699
Author(s):  
Ruifeng Wang ◽  
Xiong Wu ◽  
Yanliang Zhai ◽  
Yuxuan Su ◽  
Chenhui Liu
Keyword(s):  
Experimental Study ◽  
Mineral Water ◽  
Surrounding Rock ◽  
Mechanism Of Formation ◽  
High Quality ◽  
General Rules ◽  
Leaching Experiment ◽  
High Level ◽  
Impact Experiments

Chengde City boasts a wealth of high-quality mineral water resources characterized by a high level of strontium (Sr), a low level of sodium, and low alkalinity. In order to study the mechanism of formation of Sr-bearing mineral water in Chengde and to scientifically guide future mineral water exploration, taking three typical mineral water exploration areas in Chengde as examples, this paper studies the sources of Sr in mineral water and the general rules of its dissolution via a laboratory static leaching experiment and impact experiments, and it provides an analysis of the characteristics of typical rock samples. The research results indicate that the content of Sr in surrounding rock and the characteristics of minerals existing in surrounding rock jointly control the dissolution of Sr in water; that CO2 can promote the formation of mineral water containing Sr; and that temperature increases may boost the dissolution of Sr from carbonate minerals but also inhibit the dissolution of Sr from silicate minerals.

scholarly journals De Novo SNP Discovery and Genotyping of Iranian Pimpinella Species Using ddRAD Sequencing

Agronomy ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 1342
Author(s):  
Shaghayegh Mehravi ◽  
Gholam Ali Ranjbar ◽  
Ghader Mirzaghaderi ◽  
Anita Alice Severn-Ellis ◽  
Armin Scheben ◽  
...  
Keyword(s):  
De Novo ◽  
Genetic Relationships ◽  
Nucleotide Polymorphisms ◽  
High Quality ◽  
Genomic Resources ◽  
High Quality Snps ◽  
The Family ◽  
Double Digestion ◽  
Flanking Sequences ◽  
Downstream Analysis

The species of Pimpinella, one of the largest genera of the family Apiaceae, are traditionally cultivated for medicinal purposes. In this study, high-throughput double digest restriction-site associated DNA sequencing technology (ddRAD-seq) was used to identify single nucleotide polymorphisms (SNPs) in eight Pimpinella species from Iran. After double-digestion with the enzymes HpyCH4IV and HinfI, a total of 334,702,966 paired-end reads were de novo assembled into 1,270,791 loci with an average of 28.8 reads per locus. After stringent filtering, 2440 high-quality SNPs were identified for downstream analysis. Analysis of genetic relationships and population structure, based on these retained SNPs, indicated the presence of three major groups. Gene ontology and pathway analysis were determined by using comparison SNP-associated flanking sequences with a public non-redundant database. Due to the lack of genomic resources in this genus, our present study is the first report to provide high-quality SNPs in Pimpinella based on a de novo analysis pipeline using ddRAD-seq. This data will enhance the molecular knowledge of the genus Pimpinella and will provide an important source of information for breeders and the research community to enhance breeding programs and support the management of Pimpinella genomic resources.

scholarly journals Non-destructive handheld XRF study of archaeological composite silver objects—the case study of the late Roman Seuso Treasure

2021 ◽  
Vol 13 (5) ◽  
Author(s):  
Viktória Mozgai ◽  
Bernadett Bajnóczi ◽  
Zoltán May ◽  
Zsolt Mráv
Keyword(s):  
Fluorescence Analysis ◽  
The Body ◽  
High Quality ◽  
X Ray ◽  
Composite Objects ◽  
Mechanical Attachment ◽  
Non Destructive ◽  
Made In ◽  
Late Roman

AbstractThis study details the non-destructive chemical analysis of composite silver objects (ewers, situlas, amphora and casket) from one of the most significant late Roman finds, the Seuso Treasure. The Seuso Treasure consists of fourteen large silver vessels that were made in the fourth–early fifth centuries AD and used for dining during festive banquets and for washing and beautification. The measurements were systematically performed along a pre-designed grid at several points using handheld X-ray fluorescence analysis. The results demonstrate that all the objects were made from high-quality silver (above 90 wt% Ag), with the exception of the base of the Geometric Ewer B. Copper was added intentionally to improve the mechanical properties of soft silver. The gold and lead content of the objects shows constant values (less than 1 wt% Au and Pb). The chemical composition as well as the Bi/Pb ratio suggests that the parts of the composite objects were manufactured from different silver ingots. The ewers were constructed in two ways: (i) the base and the body were made separately, or (ii) the ewer was raised from a single silver sheet. The composite objects were assembled using three methods: (i) mechanical attachment; (ii) low-temperature, lead-tin soft solders; or (iii) high-temperature, copper-silver hard solders. Additionally, two types of gilding were revealed by the XRF analysis, one with remnants of mercury, i.e. fire-gilding, and another type without remnants of mercury, presumably diffusion bonding.

scholarly journals Method for Viewing Real-World Scenes while Recording Video

2021 ◽  
Vol 11 (10) ◽  
pp. 4617
Author(s):  
Daehee Park ◽  
Cheoljun Lee
Keyword(s):  
Real World ◽  
Video Recording ◽  
Emotional Responses ◽  
Target Object ◽  
Error Rates ◽  
User Needs ◽  
High Quality ◽  
Gradient Algorithms ◽  
Squared Error

Because smartphones support various functions, they are carried by users everywhere. Whenever a user believes that a moment is interesting, important, or meaningful to them, they can record a video to preserve such memories. The main problem with video recording an important moment is the fact that the user needs to look at the scene through the mobile phone screen rather than seeing the actual real-world event. This occurs owing to uncertainty the user might feel when recording the video. For example, the user might not be sure if the recording is of high-quality and might worry about missing the target object. To overcome this, we developed a new camera application that utilizes two main algorithms, the minimum output sum of squared error and the histograms of oriented gradient algorithms, to track the target object and recognize the direction of the user’s head. We assumed that the functions of the new camera application can solve the user’s anxiety while recording a video. To test the effectiveness of the proposed application, we conducted a case study and measured the emotional responses of users and the error rates based on a comparison with the use of a regular camera application. The results indicate that the new camera application induces greater feelings of pleasure, excitement, and independence than a regular camera application. Furthermore, it effectively reduces the error rates during video recording.

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