scholarly journals Transcriptome analysis of osmotic-responsive genes in ABA-dependent and -independent pathways in wheat (Triticum aestivum L.) roots

PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e6519 ◽  
Author(s):  
Chunxi Li ◽  
Wenli Zhang ◽  
Meng Yuan ◽  
Lina Jiang ◽  
Bo Sun ◽  
...  
Keyword(s):  
Osmotic Stress ◽  
Calcium Binding ◽  
Molecular Mechanisms ◽  
Soluble Sugars ◽  
Triticum Aestivum L ◽  
Wheat Roots ◽  
Wheat Growth ◽  
Wheat Seedlings ◽  
Yield And Quality ◽  
Trehalose Biosynthesis

Bread wheat is one of the most important crops in the world. However, osmotic stress significantly inhibits wheat growth and development, and reduces crop yield and quality. Plants respond to osmotic stress mainly through abscisic acid (ABA)-dependent and -independent pathways. In this study, root transcriptome profiles of wheat seedlings exposed to osmotic stress and exogenous ABA were analysed to identify osmotic-responsive genes belonging to the ABA-dependent or -independent pathways. We found that osmotic stress promoted proline biosynthesis in the ABA-dependent pathway, and trehalose biosynthesis is likely promoted among soluble sugars to maintain protein bioactivity under osmotic stress. In wheat roots subjected to osmotic stress, calcium ions, and glutathione exert their functions mainly through calcium-binding protein (CaM/CML) and glutathione-S-transferase, respectively, depending on both pathways. In addition, a complex relationship among phytohormones signal transduction was observed in response to osmotic stress. The findings of this study deepen our understanding of the molecular mechanisms of osmotic-stress resistance, and provide several candidate osmotic-responsive genes for further study.

Effect of 24-epibrassinolide on localization of ABA, AZP, and dehydrins in wheat plant roots during dehydration

Biomics ◽  
2020 ◽  
Vol 12 (3) ◽  
pp. 329-336
Author(s):  
A.R. Lubyanova ◽  
F.M. Shakirova ◽  
M.V. Bezrukova
Keyword(s):  
Triticum Aestivum ◽  
Wheat Germ ◽  
Wheat Plant ◽  
Triticum Aestivum L ◽  
Immunohistochemical Localization ◽  
Plant Roots ◽  
Wheat Roots ◽  
Wheat Seedlings ◽  
Apoplastic Barrier ◽  
Protective Proteins

We studied the immunohistochemical localization of abscisic acid (ABA), wheat germ agglutinin (WGA) and dehydrins in the roots of wheat seedlings (Triticum aestivum L.) during 24-epibrassinolide-pretreatment (EB-pretreatment) and PEG-induced dehydration. It was found coimmunolocalization of ABA, WGA and dehydrins in the cells of central cylinder of basal part untreated and EB-pretreated roots of wheat seedlings under normal conditions and under osmotic stress. Such mutual localization ABA and protective proteins, WGA and dehydrins, indicates the possible effect of their distribution in the tissues of EB-pretreated wheat roots during dehydration on the apoplastic barrier functioning, which apparently contributes to decrease the water loss under dehydration. Perhaps, the significant localization of ABA and wheat lectin in the metaxylem region enhances EB-induced transport of ABA and WGA from roots to shoots under stress. It can be assumed that brassinosteroids can serve as intermediates in the realization of the protective effect of WGA and wheat dehydrins during water deficit.

scholarly journals Comparative Physiological and Transcriptomic Analyses Reveal Mechanisms of Improved Osmotic Stress Tolerance in Annual Ryegrass by Exogenous Chitosan

Genes ◽  
2019 ◽  
Vol 10 (11) ◽  
pp. 853 ◽  
Author(s):  
Zhao ◽  
Pan ◽  
Zhou ◽  
Yang ◽  
Meng ◽  
...  
Keyword(s):  
Osmotic Stress ◽  
Molecular Mechanisms ◽  
Intact Cell ◽  
Lolium Multiflorum ◽  
Soluble Sugars ◽  
Annual Ryegrass ◽  
Gene Expressions ◽  
Osmotic Stress Tolerance ◽  
Pre Treatment ◽  
The Impact

Water deficit adversely affects the growth and productivity of annual ryegrass (Lolium multiflorum Lam.). The exogenous application of chitosan (CTS) has gained extensive interests due to its effect on improving drought resistance. This research aimed to determine the role of exogenous CTS on annual ryegrass in response to water stress. Here, we investigated the impact of exogenous CTS on the physiological responses and transcriptome changes of annual ryegrass variety “Tetragold” under osmotic stress induced by exposing them to 20% polyethylene glycol (PEG)-6000. Our experimental results demonstrated that 50 mg/L exogenous CTS had the optimal effect on promoting seed germination under osmotic stress. Pre-treatment of annual ryegrass seedlings with 500 mg/L CTS solution reduced the level of electrolyte leakage (EL) as well as the contents of malondialdehyde (MDA) and proline and enhanced the activities of superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), and ascorbic acid peroxidase (APX) under osmotic stress. In addition, CTS increased soluble sugars and chlorophyll (Chl) content, net photosynthetic rate (A), stomatal conductance (gs), water use efficiency (WUE), and transpiration rate (E) in annual ryegrass seedlings in response to three and six days of osmotic stress. Transcriptome analysis further provided a comprehensive understanding of underlying molecular mechanisms of CTS impact. To be more specific, in contrast of non-treated seedlings, the distinct changes of gene expressions of CTS-treated seedlings were shown to be tightly related to carbon metabolism, photosynthesis, and plant hormone. Altogether, exogenous CTS could elicit drought-related genes in annual ryegrass, leading to resistance to osmotic stress via producing antioxidant enzymes and maintaining intact cell membranes and photosynthetic rates. This robust evidence supports the potential of the application of exogenous CTS, which will be helpful for determining the suitability and productivity of agricultural crops.

scholarly journals Response of growth and antioxidant enzymes to osmotic stress in two different wheat (Triticum aestivum L.) cultivars seedlings

2012 ◽  
Vol 58 (No. 12) ◽  
pp. 534-539 ◽  
Author(s):  
G.Q. Wu ◽  
L.N. Zhang ◽  
Y.Y. Wang
Keyword(s):  
Triticum Aestivum ◽  
Antioxidant Enzymes ◽  
Osmotic Stress ◽  
Wheat Cultivar ◽  
Triticum Aestivum L ◽  
Shoot Length ◽  
The Other ◽  
Wheat Cultivars ◽  
Wheat Seedlings ◽  
Drought Tolerant

 To investigate the responses of growth and antioxidant enzymes to osmotic stress in two different wheat cultivars, one drought tolerant (Heshangtou, HST) and the other drought sensitive (Longchun 15, LC15), 15-day-old wheat seedlings were exposed to osmotic stress of –0.25, –0.50, and –0.75 MPa for 2 days. It is found that osmotic stress decreased shoot length in both wheat cultivars, whereas to a lesser degree in HST than in LC15. The contents of malondialdehyde (MDA) and the activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) of shoot in both wheat cultivars were increased by osmotic stress. It is clear that MDA contents increased less in the more drought tolerant cultivar HST than in drought sensitive one LC15. On the contrary, POD and CAT activities increased more in HST than LC15 under osmotic stress. As the activity of SOD, however, no significant differences were found between HST and LC15. These results suggest that wheat cultivar HST has higher activities of antioxidant enzymes such as POD and CAT to cope with oxidative damage caused by osmotic stress compared to sensitive LC15.  

Changes in the Ultrastructure of the Root Tip of Wheat Following Exposure to Aluminium

1994 ◽  
Vol 21 (1) ◽  
pp. 85 ◽  
Author(s):  
MLD Lima ◽  
L Copeland
Keyword(s):  
Triticum Aestivum ◽  
Prolonged Exposure ◽  
Morphological Changes ◽  
Triticum Aestivum L ◽  
Root Tip ◽  
Cortical Layers ◽  
Wheat Roots ◽  
Wheat Seedlings ◽  
Meristematic Cells ◽  
Fermentative Metabolism

Investigations have been carried out on morphological changes induced by aluminium ions in roots of wheat seedlings (Triticum aestivum L. cv. Vulcan). Lesions were evident on the surface of the roots after 4-8 h of exposure, and within 24 h there was increased vacuolation, loss of turgor, and severe cytoplasmic disorganisation in epidermal and peripheral cap cells. The central cap and cortical layers were also severely damaged by aluminium, but changes in the meristematic cells became evident only after more prolonged exposure of roots to aluminium. Mobilisation of starch in amyloplasts of peripheral and central cap cells of aluminium-stressed roots was particularly noticeable, and this was accompanied by an increase in the amount of extractable activity of starch-degrading enzymes. The possibility that the mobilisation of starch is linked to a coincident increase in fermentative metabolism in Al-stressed wheat roots is considered.

scholarly journals Molecular Mechanisms of the 1-Aminocyclopropane-1-Carboxylic Acid (ACC) Deaminase Producing Trichoderma asperellum MAP1 in Enhancing Wheat Tolerance to Waterlogging Stress

2021 ◽  
Vol 11 ◽  
Author(s):  
Mamoona Rauf ◽  
Muhammad Awais ◽  
Aziz Ud-Din ◽  
Kazim Ali ◽  
Humaira Gul ◽  
...  
Keyword(s):  
Gene Expression ◽  
Carboxylic Acid ◽  
Stomatal Conductance ◽  
Molecular Mechanisms ◽  
Acc Deaminase ◽  
Antioxidant Enzyme Activities ◽  
Trichoderma Asperellum ◽  
Wheat Growth ◽  
Wheat Seedlings ◽  
Waterlogging Stress

Waterlogging stress (WS) induces ethylene (ET) and polyamine (spermine, putrescine, and spermidine) production in plants, but their reprogramming is a decisive element for determining the fate of the plant upon waterlogging-induced stress. WS can be challenged by exploring symbiotic microbes that improve the plant’s ability to grow better and resist WS. The present study deals with identification and application of 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase-producing fungal endophyte Trichoderma asperellum (strain MAP1), isolated from the roots of Canna indica L., on wheat growth under WS. MAP1 positively affected wheat growth by secreting phytohormones/secondary metabolites, strengthening the plant’s antioxidant system and influencing the physiology through polyamine production and modulating gene expression. MAP1 inoculation promoted yield in comparison to non-endophyte inoculated waterlogged seedlings. Exogenously applied ethephon (ET synthesis inducer) and 1-aminocyclopropane carboxylic acid (ACC; ET precursor) showed a reduction in growth, compared to MAP1-inoculated waterlogged seedlings, while amino-oxyacetic acid (AOA; ET inhibitor) application reversed the negative effect imposed by ET and ACC, upon waterlogging treatment. A significant reduction in plant growth rate, chlorophyll content, and stomatal conductance was noticed, while H2O2, MDA production, and electrolyte leakage were increased in non-inoculated waterlogged seedlings. Moreover, in comparison to non-inoculated waterlogged wheat seedlings, MAP1-inoculated waterlogged wheat exhibited antioxidant–enzyme activities. In agreement with the physiological results, genes associated with the free polyamine (PA) biosynthesis were highly induced and PA content was abundant in MAP1-inoculated seedlings. Furthermore, ET biosynthesis/signaling gene expression was reduced upon MAP1 inoculation under WS. Briefly, MAP1 mitigated the adverse effect of WS in wheat, by reprogramming the PAs and ET biosynthesis, which leads to optimal stomatal conductance, increased photosynthesis, and membrane stability as well as reduced ET-induced leaf senescence.

The damage repair role of He-Ne laser on wheat exposed to osmotic stress

2010 ◽  
Vol 90 (5) ◽  
pp. 691-698 ◽  
Author(s):  
Z. -B. Qiu ◽  
J. -T. Li ◽  
M. Yue
Keyword(s):  
Triticum Aestivum ◽  
Laser Radiation ◽  
Osmotic Stress ◽  
Production Rate ◽  
Antioxidative Enzymes ◽  
Triticum Aestivum L ◽  
Wheat Seedlings ◽  
Damage Repair ◽  
Helium Neon

In order to determine the damage repair role of helium-neon (He-Ne) laser on wheat (Triticum aestivum L.) exposed to osmotic stress, 12-d-old seedlings (with two fully expanded leaves) were treated with osmotic stress using 5% (wt/vol), 10% (wt/vol) and 15% (wt/vol) polyethylene glycol (PEG 6000) treatment for 9 d. After 9 d of osmotic stress, a He-Ne laser was employed to irradiate seedlings of spring wheat for 0 min, 1 min and 3 min. Changes in the concentration of malondialdehyde (MDA), hydrogen peroxide (H2O2), glutathione (GSH), ascorbate (AsA), the production rate of superoxide radical (O2), the activities of ascorbate peroxidase (APX), peroxidase (POD), catalase (CAT), superoxide dismutase (SOD) and glutathione reductase (GR) were measured to test the effects of laser radiation. The results showed that laser radiation for 3 min conferred tolerance to osmotic stress in wheat seedlings by decreasing the concentration of MDA and the production rate of O2, and increasing the activities of SOD and APX and GSH concentration. It was suggested that those changes in MDA, O2, antioxidative enzymes and antioxidative compounds were responsible for the increase in osmotic stress tolerance observed in the experiments. Therefore, antioxidative enzymes and antioxidative compounds may participate in the repair effect of laser on seedlings under osmotic stress. This is the first investigation reporting the damage repair role of He-Ne laser on plants exposed to osmotic stress.Key words: Helium-neon laser, wheat (Triticum aestivum L.), osmotic stress, antioxidative system

Effect of aluminium on membrane potential and ion fluxes at the apices of wheat roots

2005 ◽  
Vol 32 (3) ◽  
pp. 199 ◽  
Author(s):  
Tim Wherrett ◽  
Peter R. Ryan ◽  
Emmanuel Delhaize ◽  
Sergey Shabala
Keyword(s):  
Membrane Potential ◽  
Spatial Information ◽  
Triticum Aestivum L ◽  
Ion Fluxes ◽  
Bathing Solution ◽  
Dependent Manner ◽  
Al Tolerance ◽  
Wheat Roots ◽  
Wheat Seedlings ◽  
Root Apices

Aluminium (Al) tolerance in wheat (Triticum aestivum L.) is associated with the Al-activated efflux of malate and K+ from the root apices. We tested the hypothesis that these Al-activated ion fluxes would induce changes in the membrane potential (Vm) and that these responses would differ between wheat genotypes that differ in Al tolerance. Within minutes of exposing wheat roots to 50 μm AlCl3, a significant depolarisation was measured in the Al-tolerant ET8 genotype but not in a near-isogenic, Al-sensitive genotype, ES8. We investigated the ion movements that may be responsible for these changes in Vm by measuring real-time fluxes of Cl–, H+ and K+ at the root apices of wheat seedlings using the non-invasive microelectrode ion flux estimation (MIFE) technique. Addition of 50 μm AlCl3 to the bathing solution stimulated an increase in K+ efflux and H+ influx in ET8 but not in ES8. The differences between the genotypes were sustained for 24 h and were observed only at the elongating zone and not the meristematic zone. After 24 h Al increased Cl– influx in ET8 but inhibited ES8 influx in a dose-dependent manner. These results provide new temporal and spatial information on the Al-activated ion fluxes from intact wheat plants.

scholarly journals Adaptation reactions of common wheat (Triticum aestivum L.) and emmer (T. dicoccum Schrank ex Schübl.) seedlings under osmotic stress and treatment with metal nanoparticles

2019 ◽  
Vol 6 (3) ◽  
pp. 3-13
Author(s):  
M. Musienko ◽  
Ya. Gadzalo ◽  
M. Kovalenko ◽  
L. Batsmanova ◽  
Ye. Konotop ◽  
...  
Keyword(s):  
Osmotic Stress ◽  
Metal Nanoparticles ◽  
Common Wheat ◽  
Triticum Aestivum L ◽  
Stress Conditions ◽  
Wheat Cultivars ◽  
Sod Activity ◽  
Wheat Seedlings ◽  
Oxidative Processes ◽  
Oxidative Homeostasis

Aim. To study the adaptive reactions of common wheat (Triticum aestivum L.) and emmer (T. dicoccum Schrank ex Schübl.) seedlings using the parameters of oxidative homeostasis under polyethylene glycol (PEG)-induced osmotic stress and metal (Fe, Cu, Mn, Zn) mixture nanoparticles treatment. Methods. Biochemical assays: spectrophotometric measurements of the activity of antioxidant enzymes (SOD, CAT), the content of proline and products of lipid peroxidation; mathematical statistics. Results. The state of oxidative homeostasis of common wheat (cv. Favorytka, cv. Trypilska) and emmer (cv. Holikovska) seedlings under PEG-induced osmotic stress conditions was investigated and analyzed. The development of lipid peroxidation processes under stress conditions was observed only in the cv. Trypilska seedlings. It was established that proline content in common wheat seedlings of both cultivars under stress had signifi cant (10 : 1) predominance in roots, while in roots and shoots of emmer seedlings proline content increased equally. SOD activity in the leaves of the studied cultivars under stress conditions was not changed. At the same time, an increase of SOD activity by 30 % under osmotic stress was shown in the roots of common wheat of cv. Favorytka, while it decreased by 25 % in the roots of cv. Trypilska. It was established that CAT activity in the roots of both cultivars of common wheat decreased by 25 and 38 %, respectively, whereas in emmer of cv. Holikovska this parameter increased by 35 % under osmotic stress. Presowing treatment using a colloidal solution of a mixture of biogenic metal nanoparticles contributed to the induction of SOD activity in the roots of cv. Favorytka, CAT activity in the leaves of cv. Trypilska, and CAT activity in the roots and leaves of cv. Holikovska compared to non-treated plants. It should be noted that the treatment of plants with metal nanoparticles promoted the restoration of CAT activity in the roots under osmotic stress conditions to the level of the control plants in both wheat varieties. Conclusions. It was established that osmotic stress provoked the development of oxidative processes and inhibition of the activities of antioxidant enzymes, in particular, SOD and CAT, in the seedling roots of common wheat cultivars. It was shown that emmer wheat seedlings of cv. Holikovska can maintain redox homeostasis and avoid oxidative damage under osmotic stress conditions. It was found that the seedlings of common wheat and emmer demonstrated different strategies of osmotic regulation under osmotic stress, which is confi rmed, in particular, at the level of proline accumulation. It was shown that the application of colloidal solutions of metal nanoparticles induced an antioxidant protection system and reduced the oxidative processes, which are inevitable effects of drought. The obtained results indicate that common wheat cultivars are more susceptible to drought compared to emmer wheat of cv. Holikovska.

scholarly journals Foliage application of chitosan alleviates cadmium toxicity in wheat seedlings (Triticum aestivum L)

2021 ◽  
Author(s):  
Jun Liu ◽  
Ying Xu ◽  
Haiying Zong
Keyword(s):  
Molecular Weight ◽  
Cadmium Toxicity ◽  
Soluble Sugar ◽  
Wheat Seedling ◽  
Triticum Aestivum L ◽  
Cd Stress ◽  
Wheat Growth ◽  
Wheat Seedlings ◽  
Cd Toxicity ◽  
Mda Content

Abstract Excessive cadmium (Cd) causes toxic effects on crops. The effects of chitosan (CTS) with different molecular weight (Mw) (5 kDa, 3kDa, and 1 kDa) on the growth and biochemical parameters, as well as Cd concentrations in Cd-treated wheat plants were examined in a pot experiment. The results demonstrated that foliar spraying with CTS significantly improve the wheat growth, reduce malondialdehyde (MDA) content in leaves and decrease Cd concentrations in roots and shoots of wheat seeding under Cd stress. The alleviation of Cd toxicity by CTS is probably related with the activity of antioxidant enzymes, osmotic adjustment matter and root morphology. The application of CTS enhanced the activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) in Cd-stressed wheat seedling leaves by 6.6%–13.1%, 17.2%–33.0%, and 19.6%–25.5%, respectively. Besides, exogenously applied CTS also increased the soluble protein and soluble sugar contents by 17.6%–33.8% and 30.1%–36.1% in the leaves of wheat under Cd stress. Furthermore, CTS with a molecular weight of 1 kDa was the most effective in mitigating Cd toxicity in wheat seedlings, which indicates that the activity of CTS is depend on its molecular weight. It can be concluded that the use of foliar spraying, especially with 1 kDa CTS, could have potential in reducing the damage of Cd stress.

scholarly journals Salinity Stress in Wheat (Triticum aestivum L.) in the Changing Climate: Adaptation and Management Strategies

2021 ◽  
Vol 3 ◽  
Author(s):  
Ayman EL Sabagh ◽  
Mohammad Sohidul Islam ◽  
Milan Skalicky ◽  
Muhammad Ali Raza ◽  
Kulvir Singh ◽  
...  
Keyword(s):  
Salinity Stress ◽  
Molecular Mechanisms ◽  
Climate Adaptation ◽  
Management Strategies ◽  
Wheat Yield ◽  
Triticum Aestivum L ◽  
Mitigation Strategies ◽  
Growth And Yield ◽  
Changing Climate ◽  
Yield And Quality

Wheat constitutes pivotal position for ensuring food and nutritional security; however, rapidly rising soil and water salinity pose a serious threat to its production globally. Salinity stress negatively affects the growth and development of wheat leading to diminished grain yield and quality. Wheat plants utilize a range of physiological biochemical and molecular mechanisms to adapt under salinity stress at the cell, tissue as well as whole plant levels to optimize the growth, and yield by off-setting the adverse effects of saline environment. Recently, various adaptation and management strategies have been developed to reduce the deleterious effects of salinity stress to maximize the production and nutritional quality of wheat. This review emphasizes and synthesizes the deleterious effects of salinity stress on wheat yield and quality along with highlighting the adaptation and mitigation strategies for sustainable wheat production to ensure food security of skyrocketing population under changing climate.

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