scholarly journals Effect of Ethanol Extract of Cucurbita pepo Leaves on the Lipid Profile of Wistar Albino Rats

2018 ◽  
pp. 1-7
Author(s):  
F. U. Eneh ◽  
G. C. Ugochukwu ◽  
C. M. Okoye
Keyword(s):  
Lipid Profile ◽  
Test Group ◽  
Ethanol Extract ◽  
Albino Rats ◽  
Phytochemical Investigation ◽  
Significant Difference ◽  
The Rich ◽  
Wistar Albino Rats ◽  
And Control

Aim: The role of nutrition in lipid metabolism continues to generate a lot of research interest, especially as dyslipidaemia is implicated in a host of diseases. The work investigated the effect of ethanol extract of  Curcubita pepo leaves on the lipid profile of Wister rats. Methodology: A total of 18 rats divided into three groups of 6 rats each were employed in the investigation. The first group (baseline) was sacrificed after purchase; second group (control) was fed rat chow, and the third group (test) was fed a composite feed containing rat chow and 10% extract of C. pepo leaves.  Results: The lipid profile (total cholesterol, triglycerides, LDL, HDL and VLDL) of the rats was assayed after 18 days of feeding and the result showed no significant difference (P>0.05) between the test group and control for the lipids assayed. However, there was a marked increase in the HDL level of the test group (0.500±0.057 mmol/L) compared to the control (0.268±0.043 mmol/L), it was nonetheless statistically non-significant (P=0.068).  The HDL increasing effect observed may stem from the rich phytochemistry of the leaves, as preliminary phytochemical investigation showed the leaves to contain saponin, tannin, alkaloids, flavonoids and glycosides. Conclusion: We conclude that the leaves of C. pepo may play a medicinal role in maintaining lipid homeostasis.

scholarly journals Evaluation of Anti-oxidant Enzymes, Lipid Peroxidation, Lipid Profile and Liver Function in Albino Rats Orally Administered Tartrazine

2020 ◽  
pp. 19-29
Author(s):  
Uyota Anthony Adele ◽  
Geraldine Iroh ◽  
Ojoye Ngoye Briggs ◽  
Helen Anthony Waribo ◽  
Ibioku Elekima
Keyword(s):  
Lipid Peroxidation ◽  
Liver Function ◽  
Lipid Profile ◽  
Whole Blood ◽  
Test Group ◽  
Control Group ◽  
Albino Rats ◽  
Central Vein ◽  
Significant Difference ◽  
Anti Oxidant

Aim: To evaluate the anti-oxidant enzymes, lipid peroxidation, lipid profile and liver function in albino rats orally administered tartrazine. Study Design: A total number of 63 female albino rats weighing approximately 0.2 kg were used for this study. The study was divided into two phases, phase 1 which lasted for the first 30 days, comprised of 35 rats, 20 rats were used as test group while 15 rats served as the control group. Phase 2 of the study was for 60 days and 28 rats were used with 16 as test group and 12 as the control. The test groups were orally administered with 7.5 mg/kg of tartrazine (ADI) daily over the specified periods while the control groups were not treated with tartrazine but given only food and water. Place and Duration of Study: The study was carried out in the Department of Medical Laboratory Science, Rivers State University, Port Harcourt, Nigeria within a period of 12 months (Feb., 2019 – Jan., 2020). Methodology: At the end of the study, 5 mls of whole blood specimens was collected by means of cardiac puncture into plain bottles. To obtain the serum, the whole blood samples were allowed to clot and later dislodged and spun at 3500 rpm for 10 minutes. The collected serum specimens were used to analyze SOD, MDA, GPX, ALT, GGT, ALP, TG, TCHOL, and HDL-C, while LDL-C was calculated using Friedwald equation. Results: The chronic treatment of rats with tartrazine azo food dye at the ADI dose caused an increase in MDA levels after 30 and 60 days test rats compared to the control, while TCHOL and HDL-C showed significant decrease after 30 and 60 days of treatment in the test group compared to the control group. In addition, ALT indicated significant increase in test group after 60 days of treatment compared to control group. ALP, GGT, TG, LDL-C, SOD and GPX showed no significant difference after 30, and 60 days of treatment at ADI doses. Histologic examination of the liver indicated hydropic dilation, degenerating hepatocyes and infiltration of central vein with parenchymal materials alongside kupffer cells. Conclusion: The results from this study revealed that orally administered tartrazine at the recommended ADI dose increased lipid peroxidation as seen in the elevated MDA levels. Hepatic derangements were also seen as revealed by increased ALT and histologic distortions as well fall in TCHOL and HDL-C lipid fractions.

scholarly journals Effect of Moderate Intake of Ethanol on Lipid Profile of Wistar-Albino Rats Fed High Fat Diet

2020 ◽  
Vol 2 (3) ◽  
Author(s):  
Kathiravan Vayanaperumal ◽  
Ravindran Rajan
Keyword(s):  
Lipid Profile ◽  
Blood Cholesterol ◽  
Ethanol Administration ◽  
Control Group ◽  
Albino Rats ◽  
High Fat ◽  
Group I ◽  
Significant Difference ◽  
Wistar Albino Rats ◽  
The Impact

Many evidences suggest that light to moderate alcohol drinking may provide some cardiovascular benefits. The present study examined the impact of how moderate ethanol consumption provides a protective effect against high blood cholesterol. Wistar-Albino rats were divided into a control group (I) and test groups (II, III, IV). The test groups (II, III, IV) were administered with a predefined dosage of 10%, 20% and 30% ethanol respectively following twenty days of high fat routine however, control animals were managed with normal saline for the next fifteen days. Significant difference in lipid profile parameters witnessed before and after ethanol administration between the control and test groups. Findings from the study suggests that 10% moderate ethanol is adequately least enough to reduce the risk of cardiovascular disease by improving the blood cholesterol in Wistar-Albino rats.

scholarly journals Partial Effect of Resveratrol on Wound Healing: Study on Wistar Albino Rats

2021 ◽  
Author(s):  
Tayfun Bilgiç ◽  
Nazlı Caf ◽  
Ayşe Nilhan Atsü
Keyword(s):  
Wound Healing ◽  
Blood Perfusion ◽  
Female Rats ◽  
Control Group ◽  
Albino Rats ◽  
New Therapeutic Approaches ◽  
Significant Difference ◽  
Carcinogenic Effects ◽  
Wistar Albino Rats ◽  
And Control

Abstract Purpose: There are multiple and specific phases during wound healing. Resveratrol (RSV) is a polyphenol compound found naturally in fruits. Resveratrol's antioxidant, anti-aging, cardioprotective, anti-inflammatory, and anti-carcinogenic effects have been shown. This study aims to determine the effects of RSV on perfusion, neovascularization, inflammation, and granulation stages of wound healing in addition to its antioxidant capacity over GPX and SOD.Methods: 16 female rats were divided into two groups as the RSV and control groups. Six excisions were made in each rat. Three excisions in each rat were sutured and the other 3 were left as open wounds. Topical RSV was applied to the wounds of the rats in the RSV group. No treatment was given to the control group. Blood perfusion of the wounds in both groups were measured using the PeriScan PIM 3 System Laser Doppler Blood Perfusion Imager. Punch biopsies were taken for histopathological examinations and for the evaluation of SOD, GPX, and MDA.Results: GPX level of the closed wounds belonging to the RSV group was higher compared to the control group. There was no statistically significant difference in SOD and MDA levels between the groups. Neovascularization was registered higher in the open wounds of RSV group. Conclusion: Within parameters, resveratrol has been shown to be effective in wound healing in various ways. The results of this study may shed light on new therapeutic approaches that can be used in the reversal of oxidative stress in wound-causing diseases.

Effects of Virgin Coconut Oil as Adjunct Therapy in the Treatment of Allergic Rhinitis

2016 ◽  
Vol 1 (1) ◽  
pp. 22
Author(s):  
Nazli Zainuddin ◽  
Nurul Azira Mohd Shah ◽  
Rosdan Salim
Keyword(s):  
Allergic Rhinitis ◽  
Side Effects ◽  
Coconut Oil ◽  
Test Group ◽  
Nasal Symptom ◽  
Control Group ◽  
Virgin Coconut Oil ◽  
Control Groups ◽  
Significant Difference ◽  
And Control

Introduction: The role of virgin coconut oil in the treatment of allergic rhinitis is controversial. Thus, the aim of the present study is to determine the effects of virgin coconut oil ingestion, in addition to standard medications, on allergic rhinitis. We also studied the side effects of consumption of virgin coconut oil. Methods: Fifty two subjects were equally divided into test and control groups. All subjects received a daily dose of 10mg of loratadine for 28 days. The test group was given 10ml of virgin coconut oil three times a day in addition to loratadine. The symptoms of allergic rhinitis were scored at the beginning and end of the study. Results:, the symptom score were divided into nasal and non-nasal symptom scores. Sneezing score showed a significant difference, however the score was more in control group than test group, indicating that improvement in symptom was more in control group. The rest of the nasal symptom and non-nasal symptom score showed no significant difference between test and control groups. Approximately 58% of the test subjects developed side effects from consumption of virgin coconut oil, mainly gastrointestinal side effects. Conclusion: In the present study, ingestion of virgin coconut oil does not improve the overall and individual symptoms of allergic rhinitis, furthermore it has side effects.

Investigation of the Association Between Carotid Artery Intima-Media Thickness (IMT) and Cardiac Risk Factors in Patients with Systemic Lupus Erythematosus

2020 ◽  
Vol 16 (2) ◽  
pp. 125-133
Author(s):  
Zahra Rezaieyazdi ◽  
Sima Sedighi ◽  
Masoumeh Salari ◽  
Mohammadreza H. Fard ◽  
Mahmoud R. Azarpazhooh ◽  
...  
Keyword(s):  
Risk Factors ◽  
Insulin Resistance ◽  
Lupus Erythematosus ◽  
Serum Insulin ◽  
Venous Blood ◽  
Test Group ◽  
Control Groups ◽  
Significant Difference ◽  
The Mean ◽  
And Control

Background: The relationship between SLE and traditional risk factors for cardiovascular events was evaluated. Methods: The data regarding sixty patients with SLE and 30 healthy controls (age and sex matched) were gathered using SLEDAI forms. Venous blood (10mL) from all the participants was examined for hs-CRP, homocysteine, VCAM1, CBC, anti-DNA antibody, C3, C4, low-density lipoprotein (LDL), cholesterol, FBS and triglyceride. : The IMT of carotid arteries was determined bilaterally by ultrasound. Other measurements included insulin levels via Elisa (Linco/Millipore Corp) and the HOMA-IR index for insulin resistance. Results: The mean age (in years) in the test and control groups was 28.8±10.3 (18-52) and 33.8±9.13 (18-48), respectively. Results: The mean age (in years) in the test and control groups was 28.8±10.3 (18-52) and 33.8±9.13 (18-48), respectively. : The average IMT in the test group was directly related to serum levels of VCAM1 (p<0.001), homocysteine (p<0.001), cholesterol (p<0.009), LDL (p<0.001), TG (p<0.001), and FPG (p=0.004). The association between other risk factors, insulin resistance, carotid IMT and SLEDAI, was nonexistent. Mean insulin and insulin resistance levels in all the participants were 0.43±2.06 µU/mL and 0.09±0.44, respectively. There was no significant difference between the test and control groups regarding serum insulin and insulin resistance levels (p=0.42 and p=0.9, respectively). None of the risk factors, such as hsCRP, VCAM1, or homocysteine, were shown to be related to insulin resistance (p=0.6, p=0.6, p=0.09, respectively). Conclusion:: Our findings did not show an increase in the prevalence of atherosclerosis in patients with SLE. There was no association between IMT and insulin resistance. However, the former was associated with FPG, total cholesterol, LDL, TG, homocystein and VCAM1.

Role of Nutrition Counseling and Lifestyle Modification in Managing Prediabetes

2021 ◽  
pp. 037957212110254
Author(s):  
Harleen Kaur ◽  
Neerja Singla ◽  
Rohini Jain
Keyword(s):  
Hemoglobin A1c ◽  
Lifestyle Modification ◽  
Fasting Blood Glucose ◽  
Nutrition Intervention ◽  
Control Group ◽  
Population Prevalence ◽  
Significant Difference ◽  
And Control ◽  
Experimental Group

Objective: India is the second country after China having the highest population prevalence of diabetes. Several research studies investigating diabetes have been done, but not much work has been done on prediabetes. The purpose of this study was to investigate the effect of nutrition and lifestyle modification on prediabetic females. Methods: A total of 120 prediabetic females from Ludhiana city were divided into 2 matched groups: control group (n = 60) and experimental group (n = 60). Impact of nutrition intervention for dietary and lifestyle modification (for 3 months) was assessed on the anthropometric, dietary, biochemical parameters, and diabetes risk score of the experimental group and control group (no intervention). Results: All the selected 120 subjects completed the study (experimental group = 60; control group = 60). There was significant difference in the changes between the 2 groups throughout the study. The fasting blood glucose and glycated hemoglobin A1c levels of the experimental group subjects reduced significantly ( P ≤ .01). However, no change was observed among the control group subjects. The lipid profile of the experimental group showed a significant improvement ( P ≤ .01). Conclusion: Nutrition counselling of the prediabetics regarding dietary and lifestyle modification is recommended so as to improve their metabolic control, thus preventing them from being diabetics.

scholarly journals Four Weeks of Aerobic Training Affects Cardiac Tissue Matrix Metalloproteinase, Lactate Dehydrogenase and Malate Dehydrogenase Enzymes Activities, and Hepatorenal Biomarkers in Experimental Hyperhomocysteinemia in Rats

2021 ◽  
Vol 22 (13) ◽  
pp. 6792
Author(s):  
Dusan Todorovic ◽  
Marija Stojanovic ◽  
Ana Medic ◽  
Kristina Gopcevic ◽  
Slavica Mutavdzin ◽  
...  
Keyword(s):  
Physical Activity ◽  
Lactate Dehydrogenase ◽  
Malate Dehydrogenase ◽  
Subcutaneous Injection ◽  
Cardiac Tissue ◽  
Albino Rats ◽  
Wistar Albino Rats ◽  
And Control ◽  
Tissue Matrix ◽  
Increased Activity

The aim of this study was to investigate the effect of the application of homocysteine as well as its effect under the condition of aerobic physical activity on the activities of matrix metalloproteinases (MMP), lactate dehydrogenase (LDH) and malate dehydrogenase (MDH) in cardiac tissue and on hepato-renal biochemical parameters in sera of rats. Male Wistar albino rats were divided into four groups (n = 10, per group): C: 0.9% NaCl 0.2 mL/day subcutaneous injection (s.c.); H: homocysteine 0.45 µmol/g b.w./day s.c.; CPA saline (0.9% NaCl 0.2 mL/day s.c.) and a program of physical activity on a treadmill; and HPA homocysteine (0.45 µmol/g b.w./day s.c.) and a program of physical activity on a treadmill. Subcutaneous injection of substances was applied 2 times a day at intervals of 8 h during the first two weeks of experimental protocol. Hcy level in serum was significantly higher in the HPA group compared to the CPA group (p < 0.05). Levels of glucose, proteins, albumin, and hepatorenal biomarkers were higher in active groups compared with the sedentary group. It was demonstrated that the increased activities of LDH (mainly caused by higher activity of isoform LDH2) and mMDH were found under the condition of homocysteine-treated rats plus aerobic physical activity. Independent application of homocysteine did not lead to these changes. Physical activity leads to activation of MMP-2 isoform and to increased activity of MMP-9 isoform in both homocysteine-treated and control rats.

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