scholarly journals Partial Effect of Resveratrol on Wound Healing: Study on Wistar Albino Rats

2021 ◽  
Author(s):  
Tayfun Bilgiç ◽  
Nazlı Caf ◽  
Ayşe Nilhan Atsü
Keyword(s):  
Wound Healing ◽  
Blood Perfusion ◽  
Female Rats ◽  
Control Group ◽  
Albino Rats ◽  
New Therapeutic Approaches ◽  
Significant Difference ◽  
Carcinogenic Effects ◽  
Wistar Albino Rats ◽  
And Control

Abstract Purpose: There are multiple and specific phases during wound healing. Resveratrol (RSV) is a polyphenol compound found naturally in fruits. Resveratrol's antioxidant, anti-aging, cardioprotective, anti-inflammatory, and anti-carcinogenic effects have been shown. This study aims to determine the effects of RSV on perfusion, neovascularization, inflammation, and granulation stages of wound healing in addition to its antioxidant capacity over GPX and SOD.Methods: 16 female rats were divided into two groups as the RSV and control groups. Six excisions were made in each rat. Three excisions in each rat were sutured and the other 3 were left as open wounds. Topical RSV was applied to the wounds of the rats in the RSV group. No treatment was given to the control group. Blood perfusion of the wounds in both groups were measured using the PeriScan PIM 3 System Laser Doppler Blood Perfusion Imager. Punch biopsies were taken for histopathological examinations and for the evaluation of SOD, GPX, and MDA.Results: GPX level of the closed wounds belonging to the RSV group was higher compared to the control group. There was no statistically significant difference in SOD and MDA levels between the groups. Neovascularization was registered higher in the open wounds of RSV group. Conclusion: Within parameters, resveratrol has been shown to be effective in wound healing in various ways. The results of this study may shed light on new therapeutic approaches that can be used in the reversal of oxidative stress in wound-causing diseases.

scholarly journals Doxorubicin Deteriorates the Histomorphology of the Kidneys of Albino Rats

2017 ◽  
Vol 15 (1) ◽  
pp. 40-43
Author(s):  
Anup Pandeya ◽  
Chandra Bhushan Jha ◽  
Smriti Karki ◽  
Gajendra Prashad Rauniar
Keyword(s):  
Body Weight ◽  
The Body ◽  
Female Rats ◽  
Control Group ◽  
Albino Rats ◽  
Final Body Weight ◽  
Histological Features ◽  
Male And Female Rats ◽  
Significant Difference ◽  
And Control

Background and Objectives: Nephrotoxicity is one of the limiting factors for using doxorubicin as an anticancer chemotherapeutic. Reactive oxygen species and cytokines have been implicated in the nephrotoxicity induced by doxorubicin. The main objective of the present study is to identify and compare the histomorphological features in kidneys of albino rats and gross morphological features such as weight of rats and weight of the kidneys due to administration of doxorubicin. Materials and Methods: In the study, albino rats were taken as the animal model. Sixty animals were taken as the sample size. They were divided into two equal groups: experimental (n=30) and control (n=30). Rats of experimental group were treated with anticancer drug doxorubicin at a single intraperitoneal dose of 10 mg/kg body weight while the Control group of rats received a similar volume of 0.9% normal saline. The ethical clearance was taken prior to the research from IERB committee BPKIHS Dharan.  Results: Our results showed that there was high effect of drug in experimental groups of rats. It was seen that there was significant decrease in the body weight and weight of kidneys. The final body weight and kidney weight between experimental and control group showed the significant difference. Similarly there were no significant differences in the normal architecture between the male and female rats. The normal renal histological features were seen on the kidneys in the control group whereas the rats intervened with the drug had some disrupted histological features which reveal the toxicity of the drugs in the kidneys. Conclusion: The study showed toxicity of the drug in the kidneys of experimental groups of rats irrespective of gender and suggest that doxorubicin causes significant loss of the body weight and weight of kidneys and causes the disruption in the normal histological features.

Evaluation of the Effect of Platelet-Rich Fibrin on Wound Healing at Split-Thickness Skin Graft Donor Sites: A Randomized, Placebo-Controlled, Triple-Blind Study

2020 ◽  
pp. 153473461990043 ◽  
Author(s):  
Mojtaba Vaheb ◽  
Maryam Karrabi ◽  
Mahtab Khajeh ◽  
Alireza Asadi ◽  
Ehsan Shahrestanaki ◽  
...  
Keyword(s):  
Wound Healing ◽  
Chronic Wounds ◽  
Outcome Evaluation ◽  
Control Group ◽  
Platelet Rich Fibrin ◽  
Thickness Skin ◽  
Split Thickness Skin Grafting ◽  
Significant Difference ◽  
The Mean ◽  
And Control

Split-thickness skin grafting (STSG) is widely used to heal wounds resulting from trauma, burns, and chronic wounds. This study aimed to determine the true effect of platelet-rich fibrin (PRF) on patients with burn wounds requiring STSG during treatment of donor wounds. This randomized, triple-blind clinical trial was conducted on patients who referred to the burn ward of Vasei Hospital of Sabzevar, Iran, from May 2017 to May 2018. The donor site was randomly divided into 2 groups: PRF and control (Vaseline petrolatum gauze) using Vaseline gauze. In the intervention group, the PRF gel was applied to the wound and covered with Vaseline gauze and wet dressing. Conversely, only Vaseline gauze and wet dressing were applied to the control group. Outcome evaluation was conducted using paired t test and Wilcoxon signed rank-sum test, as appropriate, on days 8 and 15. The mean age of the patients was 33.10 ± 2.60 years, and 51.50% were male. The mean wound healing time in the PRF and control groups was 11.80 ± 3.51 and 16.30 ± 4.32 days, respectively ( P < .001). The PRF group showed significantly higher wound healing rates than the control group at 8 and 15 days dressing ( P < .001 and P < .001, respectively). Moreover, the mean wound healing for all wound healing indices diagnosed by 2 specialists in PRF was higher than control group on days 8 and 15 ( P < .001). We found a statistically significant difference on days 8 and 15 regarding the mean pain levels between the 2 groups ( P < .001). The findings showed that PRF can significantly increase the time and rate of donor wound healing compared with conventional treatment and also reduce the severity of pain.

scholarly journals Pharmacological Investigation of the Wound Healing Activity of Cestrum nocturnum (L.) Ointment in Wistar Albino Rats

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
Hemant Kumar Nagar ◽  
Amit Kumar Srivastava ◽  
Rajnish Srivastava ◽  
Madan Lal Kurmi ◽  
Harinarayan Singh Chandel ◽  
...  
Keyword(s):  
Wound Healing ◽  
Ethanolic Extract ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Albino Rats ◽  
Healing Effect ◽  
Wound Model ◽  
Wound Healing Effect ◽  
Wistar Albino Rats

Objectives. The present study was aimed at investigating the wound healing effect of ethanolic extract of Cestrum nocturnum (L.) leaves (EECN) using excision and incision wound model. Methods. Wistar albino rats were divided into five groups each consisting of six animals; group I (left untreated) considered as control, group II (ointment base treated) considered as negative control, group III treated with 5% (w/w) povidone iodine ointment (Intadine USP), which served as standard, group IV treated with EECN 2% (w/w) ointment, and group V treated with EECN 5% (w/w) ointment were considered as test groups. All the treatments were given once daily. The wound healing effect was assessed by percentage wound contraction, epithelialization period, and histoarchitecture studies in excision wound model while breaking strength and hydroxyproline content in the incision wound model. Result. Different concentration of EECN (2% and 5% w/w) ointment promoted the wound healing activity significantly in both the models studied. The high rate of wound contraction (P<0.001), decrease in the period for epithelialization (P<0.01), high skin breaking strength (P<0.001), and elevated hydroxyproline content were observed in animal treated with EECN ointments when compared to the control and negative control group of animals. Histopathological studies of the EECN ointments treated groups also revealed the effectiveness in improved wound healing. Conclusions. Ethanolic extract of Cestrum nocturnum (EECN) leaves possesses a concentration dependent wound healing effect.

scholarly journals Preliminary Study on Wound Healing Activity of Propolis in Albino Rats

2016 ◽  
Vol 1 (07) ◽  
Author(s):  
A.Timucin ATAYOGLU ◽  
Sibel SILICI
Keyword(s):  
Wound Healing ◽  
Treated Group ◽  
Control Group ◽  
Albino Rats ◽  
Wound Model ◽  
In Vitro Antibacterial Activity ◽  
Group 3 ◽  
Group 2 ◽  
Wistar Albino Rats

Background: Infection can lead to delayed wound healing. Recently it has been shown that propolis which is used in complementary medicine has an antibacterial and anti-inflammatory effect. The aim of this study is to determine whether propolis may contribute to wound healing. Material and Methods: Twenty-one male Wistar albino rats were randomly divided into three groups. Group1 and Group 2 were topically treated with propolis ointment and Thiocillin® oinment, respectively while Group 3 was the control group. On incision wound model, Thiocillin® and propolis ointments were applied on wound sites once daily for 30 days and the mean epidermal thickness (MET) at the 30th day was compared while antimicrobial activity of propolis was studied against different pathogens as well. Results: Propolis exhibited in vitro antibacterial activity against Staphylococcus aureus, Escherichia coli, Streptococcus sp. and Pseudomonas sp. It is observed that the MET in the groups of Propolis ointment and Thiocillin® ointment were significantly greater than that of the control group, while the MET in the group of propolis ointment was significantly greater than that of Thiocillin® ointment treated group. Conclusion: Propolis is effective in wound healing. Further study in-depth is necessary to probe into clinical correlation.

scholarly journals Ellagic Acid Activity in Healing Process of Incision Wound on Male Albino Rats (Rattus norvegicus)

2017 ◽  
Vol 3 (6) ◽  
pp. 224
Author(s):  
Hardany Primarizky ◽  
Wiwik Misaco Yuniarti ◽  
Bambang Sektiari Lukiswanto
Keyword(s):  
Wound Healing ◽  
Treatment Group ◽  
Ellagic Acid ◽  
Healing Process ◽  
Control Group ◽  
Albino Rats ◽  
Wound Healing Process ◽  
Treatment Groups ◽  
Significant Difference ◽  
Incision Wound

This study was conducted to prove whether ellagic acid can be used as a regulator of incision wound healing process in male albino rats as experimental animals. A total of 24 male healthy rats based on physical examination, aged at 3 months old and weight at 150-180 grams used in this study. Rats were divided into three groups and each group has 8 replications, group of control (P0) has no incision wound and no medication, treatment group (P1) has incision wound and treated with Betadine® and (P2) has incision wound and treated with 2,5 % ellagic acid. The variable that observed are histopathology changes (collagen, PMN, angiogenesis and fibrosis) from the male albino rats. The samples collection was taken at day 15 and data were collected and analyzed by Analysis of Variance (ANOVA) test. The result of collagen and PMN cells score in the control group (P0) were 1.80 ± 0.16 and 1.6 ± 0.08 with mild angiogenesis and no fibrosis. The results in the treatment (P2) group, where the collagen and PMN scores were 1.60 ± 0.08 and 1.5 ± 0.8, accompanied by mild angiogenesis and fibrosis. Meanwhile, in the treatment (P1) group, the results of scoring of collagen and PMN were lower than the other groups, 0, 80 ± 0.08 and 0.6 ± 0.16 accompanied by moderate angiogenesis and fibrosis. There was a significant difference of collagen scoring, PMN cells number, fibrosis and angiogenesis among the treatment groups (p<0.05). P1 treatment group has significant different with treatment groups P0 and P2 (p<0.05), but there is no significant difference between treatment groups P0 and P2 (p>0.05).  From the results, it can be concluded that the topical application of ellagic acid ointment was proved to provide healing process on incision wound. Topical administration of ellagic acid ointment for 14 days was able to improve the skin condition and promoted the wound healing.Keywords: Ellagic acid ; wound healing ; collagen ; angiogenesis ; fibrosis ; anti-inflammatory ; antioxidant ; antibacterial.

scholarly journals Wound Healing Study of Panchavalkal Ointment in Wistar Albino Rats

2021 ◽  
pp. 110-116
Author(s):  
Vaikhari Dhurve ◽  
Pravin Jawanjal ◽  
Mukesh Naria ◽  
Tukaram Dudhamal ◽  
Minal Kalambe ◽  
...  
Keyword(s):  
Wound Healing ◽  
Treated Group ◽  
Control Group ◽  
Albino Rats ◽  
Wound Area ◽  
Mean Values ◽  
Wistar Strain ◽  
The Mean ◽  
Wistar Albino Rats ◽  
Wound Healing Activity

Introduction: Panchavalkal is a well-known Ayurvedic poly-herbal formulation that has been reported to be used against inflammation, to clean ulcer, wound. Aims and Objectives: To investigate the wound healing activity of Panchavalkal ointment. Materials and Methods: Wistar strain albino rats of either sex weighing 200±20 g were used for the experiments divided in four groups each consisted of six rats.  Statistical Analysis: One-way analysis of variance (ANOVA) was used to compare the mean values of quantitative variables among the groups followed by Dunnett’s multiple ‘t’ test Observations & Results: Sesame oil and Panchvalkal ointment showed almost similar wound healing effects in comparison to control group. Discussion: Panchavalkal ointment showed statistically highly significant percentage of contraction of excision wound area compared to the normal control. Epithelization period was significantly decreased in oil and Panchavalkal ointment treated group. Conclusion: Panchavalkal ointment decreased the pain, tenderness, redness and swelling that helps to control infection and enhanced the rate of wound healing in albino rats.

scholarly journals Investigating the effect of Sesame Ointment on Wound healing of Episiotomy

2018 ◽  
Vol 9 (3) ◽  
pp. 182-184
Author(s):  
Najmeh Amani Babadi ◽  
Masoomeh Kheirkhah ◽  
Faraz Mojab ◽  
Hamid Haghani
Keyword(s):  
Wound Healing ◽  
Intervention Group ◽  
Control Group ◽  
P Value ◽  
Significant Difference ◽  
The Mean ◽  
Randomized Control ◽  
Spss Software ◽  
And Control

Episiotomy is one of the most common midwifery interventions method for preventing injuries to the pelvic floor during the delivery process. Traditional medicine has a special place in improving the quality of postpartum care. Sesame is one of the herbs with anti-inflammatory, anti-bacterial and antioxidant activity.  This study was evaluate the effect of sesame ointment on episiotomy healing. Methods: This randomized control clinical trial was performed on 104 eligible women. The samples were block randomly assigned to one of the groups Intervention and control. Samples were used sesame and placebo ointment for epizootics from 4 hours after delivery for ten days every 8 hours. Clinical evaluation of episiotomy ulcer was performed 4 hours, 7 and 10 days after delivery with using REEDA tool. SPSS software version 16 was used for data analysis. P value less than 0.05 was considered significant. Results: The average of wound healing rate was 7 days after episiotomy in the intervention group 0.09 ± 0.29 and in the control group was 0.73 ± 0.44. Independent t-test showed that the two groups had a significant difference (p <0.001).Healing of the wound 10 days after episiotomy showed that the mean scores in the control group (0.4 ± 0.49) and in the intervention group (0.02 ± 0.13), healing in the intervention group significantly decreased from the control group (p <0.001). Conclusion: Sesame ointment can be used as a pain relief and accelerator for episiotomy healing.  

Stereological examination of effects of ethanol on optic nerve in experimental alcohol model

2019 ◽  
Vol 38 (5) ◽  
pp. 610-615 ◽  
Author(s):  
T Igit ◽  
N Colcimen
Keyword(s):  
Optic Nerve ◽  
Control Group ◽  
Albino Rats ◽  
Optic Chiasma ◽  
Ethanol Group ◽  
Time Period ◽  
Examination Methods ◽  
Wistar Albino Rats ◽  
Induced Changes ◽  
And Control

The objective of the present study was to perform histological and stereological examination of alcohol-induced changes in the optic nerve, considered an extension of white matter of the brain, in rats. This study included 20 male Wistar albino rats aged 60 days and weighing 190–220 g. The rats were divided into three groups: ethanol ( n = 7), maltodextrin ( n = 7), and control ( n = 6) groups. The ethanol group was administered ethanol at a dose of 6.4% (v/v) instead of water for 18 days; the maltodextrin group received maltodextrin for the same time period, and the control group was the sham group. At the end of the experiment, a 0.5-mm long section of the optic nerve starting from the optic chiasma was dissected and examined with routine microscopic histological examination methods. The modified Cavalieri method was used for stereological measurement. Total tissue area ratios were calculated with a point grid provided by the Shtereom 1.5 software package. The statistical comparison of the groups revealed that the ethanol group had a significant reduction in the number of axons and sheath area of the optic nerve compared to the control and maltodextrin groups ( p < 0.017, p < 0.022, respectively). These results indicate the toxic effects of ethanol on the optic nerve.

scholarly journals Effect of Ethanol Extract of Cucurbita pepo Leaves on the Lipid Profile of Wistar Albino Rats

2018 ◽  
pp. 1-7
Author(s):  
F. U. Eneh ◽  
G. C. Ugochukwu ◽  
C. M. Okoye
Keyword(s):  
Lipid Profile ◽  
Test Group ◽  
Ethanol Extract ◽  
Albino Rats ◽  
Phytochemical Investigation ◽  
Significant Difference ◽  
The Rich ◽  
Wistar Albino Rats ◽  
And Control

Aim: The role of nutrition in lipid metabolism continues to generate a lot of research interest, especially as dyslipidaemia is implicated in a host of diseases. The work investigated the effect of ethanol extract of  Curcubita pepo leaves on the lipid profile of Wister rats. Methodology: A total of 18 rats divided into three groups of 6 rats each were employed in the investigation. The first group (baseline) was sacrificed after purchase; second group (control) was fed rat chow, and the third group (test) was fed a composite feed containing rat chow and 10% extract of C. pepo leaves.  Results: The lipid profile (total cholesterol, triglycerides, LDL, HDL and VLDL) of the rats was assayed after 18 days of feeding and the result showed no significant difference (P>0.05) between the test group and control for the lipids assayed. However, there was a marked increase in the HDL level of the test group (0.500±0.057 mmol/L) compared to the control (0.268±0.043 mmol/L), it was nonetheless statistically non-significant (P=0.068).  The HDL increasing effect observed may stem from the rich phytochemistry of the leaves, as preliminary phytochemical investigation showed the leaves to contain saponin, tannin, alkaloids, flavonoids and glycosides. Conclusion: We conclude that the leaves of C. pepo may play a medicinal role in maintaining lipid homeostasis.

scholarly journals Assessment of Laser Biostimulation in Induction of Ovulation

2018 ◽  
Vol 6 (7) ◽  
pp. 1193-1198
Author(s):  
Mohamed A. El-Nourya ◽  
Mahmoud B. El-Begawyb ◽  
Mona E. Morsyc ◽  
Ehab M. Al Sherbiny
Keyword(s):  
Clomiphene Citrate ◽  
Study Groups ◽  
Control Group ◽  
Albino Rats ◽  
Serum Estradiol ◽  
Serum Progesterone ◽  
Induction Of Ovulation ◽  
Significant Difference ◽  
Conventional Medical Therapy ◽  
Wistar Albino Rats

AIM: This study aimed to evaluate a new modality of low power laser biostimulation in rat ovaries, in comparison with the conventional medical therapy by clomiphene citrate that depends on up-regulation of the hypothalamic-pituitary-ovarian axis to induce ovulation.STUDY DESIGN: A Prospective experimental study carried out from January 2014 to February 2016.SETTING: University-based photobiology laboratory.MATERIALS AND METHODS: Seventy-two (72) Female-Wistar Albino rats were used in this study, divided into three groups: 17 rats used as a control group, 19 rats received clomiphene citrate (Clomid group), 36 rats exposed to diode laser 660 nm wavelength (laser group).RESULTS: Biochemical assessment of serum Estradiol and serum Progesterone was done in the three study groups. Serum Estradiol & Progesterone levels were statistically significantly higher in clomiphene and laser treated groups than non-interventional controls, while no significant difference between clomiphene and laser groups as regard of both hormones.CONCLUSION: This study shows that ovarian laser biostimulation is a new encouraging method for induction of ovulation, at least in animals. This had been proven biochemically by a significant increase in serum Estradiol and serum Progesterone.

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