Nutritional Evaluation of Brillantaisia patula Leaves

Aim: To determine the nutritional and anti-nutritional compositions of Brillantaisia patula leaves using standard analytical methods. Place and Duration of Study: The proximate, mineral and anti-nutritional compositions were determined in the chemistry laboratory of Ekiti State University, Ado – Ekiti while the amino acid was determined at the Analytical Laboratory of Multi-Environmental Management Consultant, Lagos, Nigeria. Methodology: The proximate composition was carried out using the methods of Association of Official Chemists (AOAC) while mineral and anti-nutritional compositions were determined using standard analytical methods. Amino acid analysis was carried out through ion exchange chromatography (IEC) using the Technicon Sequential Multisample (TSM) Amino Acid Analyser. Results: The proximate composition ranged from 3.18% (crude fat) to 38.6% (carbohydrate). The major mineral constituents of the sample were: P (1061 mg/100g), K (874 mg/100g), Ca (799 mg/100g), Na (82.6 mg/100g) and Mg (24.3 mg/100g) while the minor mineral constituents were: Fe (26.9 mg/100g), Zn (7.7 mg/100g) and Mn (6.05 mg/100g). The evaluated anti-nutritional contents were: 6.71 mg/g oxalate, 5.37 % saponin, 1.0 mg/100g tannin and 4.72 mg/kg cyanide. Additional results showed that the leaves contained eighteen amino acids with values ranging from 0.504 g/100g cp (tryptophan) to 14.0 g/100g cp (glutamic acid). The value of the total essential amino acids (TEAA) with histidine was 45.6g/100g cp while the total non-essential amino acid (TNEAA) was evaluated to be 46.5 g/100g cp. Conclusion: Brillantaisia patula leaves could be utilized as a good source of essential amino acids and important mineral elements.

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Proximate composition, mineral content and amino acid profile of Irvingia gabonensis O’Rorke baill leaf

International Journal of Scientific World ◽

10.14419/ijsw.v5i1.6969 ◽

2016 ◽

Vol 5 (1) ◽

pp. 23

Author(s):

Efosa Ewere ◽

Oboso Etim ◽

Usunomena Usunobun

Keyword(s):

Amino Acids ◽

Amino Acid ◽

Proximate Composition ◽

Essential Amino Acids ◽

Amino Acid Profile ◽

Antinutritional Factors ◽

World Health ◽

Isoleucine Leucine ◽

Irvingia Gabonensis ◽

Mineral Analyses

Several plants are utilized for medicinal and nutritional purposes. Irvingia gabonensis O’Rorke Baill leaf is used in herbal medicine for treatment of a number of ailments. This study was therefore carried out to investigate the proximate composition, antinutritional factors, mineral composition and amino acid profile of Irvingia gabonensis O’Rorke Baill leaf. The proximate and antinutritional factors analyses were done using standard procedures. The mineral analyses were done using flame photometry, titrimetic method, molybdo vanadate method and atomic absorption spectrophotometry and the amino acid profile was done with the aid of Applied Biosystems PTH amino acid analyzer. Results of proximate analyses were carbohydrates (75.15±1.29 %), protein (11.43±1.07 %), fat (1.99±0.74 %), fibre (4.89±0.61 %), ash (6.71±0.28 %), moisture (5.12±0.03 %) and caloric value (364.30±5.95 Kcal). Antinutrients (phytate, oxalate and cyanide) levels in the leaf were also very low. Results from mineral analyses obtained revealed that the leaf is also a very rich source of calcium, potassium, sodium, magnesium and so on. Compared with the World health organization (WHO) standards, results of the amino acid profile showed that the leaf is very rich in isoleucine, leucine, lysine, phenylalanine, threonine, valine and tyrosine which are nutritionally essential amino acids. Furthermore, extraction of the leaf using ethanol reduced the levels of these amino acids but not below the recommended WHO standard levels for most of the essential amino acids. Irvingia gabonensis O’Rorke Baill leaf is therefore a potential source of key nutrients.

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The Proximate Composition, Amino Acid Profile and Chemical Indices in Five Freshwater Fishes from Tiga Dam Reservoir, Nigeria

Croatian Journal of Fisheries ◽

10.2478/cjf-2019-0009 ◽

2019 ◽

Vol 77 (2) ◽

pp. 87-92

Author(s):

Audu Michael Elaigwu

Keyword(s):

Amino Acids ◽

Amino Acid ◽

Acid Content ◽

Proximate Composition ◽

Crude Protein ◽

Amino Acid Content ◽

Essential Amino Acids ◽

Amino Acid Profile ◽

High Energy ◽

Dam Reservoir

Abstract The study assesses the proximate composition, amino acid profile and its content and chemical indices of the sun-dried Schilbe mystus, Bagrus bayad, Oreochromis niloticus, Clarias anguillaris and Petrocephalus bane bane from Tiga Dam Reservoir, Nigeria. The proximate composition varied significantly (p<0.05) in all five species of fish and these ranged as follows: Moisture (4.79 - 9.52 g/100 g), Crude Protein (42.20 - 57.71 g/100 g), Ash Content (0.90 - 12.51 g/100 g), Ether Extract (3.41 - 9.93 g/100 g), Crude Fibre (0.62 - 5.08 g/100 g), Nitrogen Free Extract (12.28 - 42.70 g/100 g) and Dry Matter (90.48 - 95.21 g/100 g). The amino acid also differed significantly (p<0.05). The nine essential amino acids found in the five species of fish were Lysine (4.21 - 6.34 g/100 g), Histidine (1.96 - 4.30 g/100 g), Arginine (5.80 - 8.21 g/100 g), Threonine (1.93 - 5.05 g/100 g), Valine (2.91 - 5.53 g/100 g), Methionine (1.74 - 3.80 g/100 g), Isoleucine (2.04 - 3.37 g/100 g), Leucine (3.64 - 7.18 g/100 g) and Phynylalanine (1.90 - 4.23 g/100 g). Whereas, the eight non-essential amino acids included: Serine (2.12 - 5.22 g/100 g), Glutamic acid (13.24 - 16.30 g/100 g), Proline (3.12 - 6.29 g/100 g), Glycine (4.20 - 9.08 g/100 g), Alanine (5.00 - 6.36 g/100 g), Cysteine (0.94 - 1.24 g/100 g), Tyrosine (2.33 - 3.33 g/100 g) and Aspartic acid (6.34 - 11.01 g/100 g). P. bane bane was first in terms of crude protein; S. mystus had the highest lipid and essential amino acid content. Also, C. anguillaris recorded the highest calorific value and best amino acid content. Thus, P. bane bane can serve as a source of animal protein to balance deficiencies in humans. Both S. mystus and C. anguillaris can be used as a nutrient base for high energy food and oil in the food industry.

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Fortification of Ogi with Whey Increases Essential Amino Acids Content of Fortified Product

International Scholarly Research Notices ◽

10.1155/2017/7450845 ◽

2017 ◽

Vol 2017 ◽

pp. 1-3

Author(s):

J. O. Omole ◽

O. M. Ighodaro ◽

O. Durosinolorun

Keyword(s):

Amino Acids ◽

Amino Acid ◽

Proximate Composition ◽

Cheese Whey ◽

Food Product ◽

Essential Amino Acids ◽

Amino Acid Profile ◽

Usual Procedure ◽

Normal Maize ◽

Typical Food

The inability of humans to synthesize essential amino acids (EAA) necessitates the need to increase the levels of these nutrient molecules in certain foods in which they are deficient. Maize ogi is a typical food product for both infants and adults in Africa, but with poor EAA content. This study therefore sought to assess the possibility of increasing the EAA content in maize ogi by processing it with cheese whey instead of water. Maize ogi and whey-fortified ogi were prepared by the usual procedure of grain soaking, milling, and drying. Samples from both treatments were subjected to proximate composition and amino acid profile analyses using Waters 616/626 LC (HPLC) instrument. L-lysine, L-trytophan, and L-methionine contents in maize ogi remarkably increased from 0.52, 0.15, and 0.90 mg/100 gm sample, respectively, to 0.90, 240, and 1.320 mg/100 gm sample in whey-fortified ogi. There were also significant increases in other EAA contents of whey-fortified ogi relative to its counterpart (normal maize ogi). The sum increase in EAA contents (9,405 mg) correlates with the increase in protein (1 gm) per gram sample. This study demonstrates that cheese whey increases EAA content in maize ogi and suggests that whey-fortified maize ogi may be a preferred alternative to water processed maize ogi.

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AMINO ACID PROFILE AND BIOACTIVE COMPOUNDS OF SEAHORSE Hippocampus comes

Jurnal Ilmu dan Teknologi Kelautan Tropis ◽

10.29244/jitkt.v9i2.19295 ◽

2018 ◽

Vol 9 (2) ◽

pp. 605-617

Author(s):

Evi Maya Sari ◽

Mala Nurilmala ◽

Asadatun Abdullah

Keyword(s):

Amino Acids ◽

Amino Acid ◽

Bioactive Compounds ◽

Proximate Composition ◽

Ethanol Extract ◽

Essential Amino Acids ◽

Amino Acid Profile ◽

Proximate Analysis ◽

Ash Content ◽

Phytochemical Analysis

Seahorse is one of the marine living resources usually used as ornamental fish, traditional medicinal materials, and souvenirs. The purpose of the study was to determine the proximate composition of wet and dry seahorses, determine the profile of amino acid hydrolyzate and powder of seahorses, and determines to content of bioactive compounds from the ethanol extract of seahorses on qualitatively. The sample of this study is seahorses obtained from nature. Prviously, seahorses were morphometric identified, subsequently, seahorses were made of the powder, hydrolyzate, and ethanol extract. Several analyzes used were qualitative analysis of proximate, amino acid, and phytochemical analysis. Morphometric identification results indicate that the type is Hippocampus comes. The proximate composition is water content is 66.16 ± 0.33% (wet) and 10.33 ± 0.16% (dry), ash content of 9.55 ± 0.15% (wet) and 9.65 ± 0.16% (dry), lipid content 1.18 ± 0.23% (wet) and 4.89 ± 0.37% (dry), protein content of 22.73 ± 0.17% (wet) and 69.83 ± 0.31% (dry), carbohydrate 0.39 ± 0.23 (wet) and 5.50 ± 0.34 (dry). The amino acid composition both on hydrolyzate and powder comprising 9 essential amino acids are lysine, leucine, isoleucine, phenylalaline, valine, methionine, histidine, arginine, and threonine and 6 non essential amino acids are tyrosine, alanine, glycine, serine, glutamic acid, and aspartic acid. The results of identification of bioactive compounds is flavonoids, triterpenoids, steroids, saponins, and phenol of hydroquinone. Keywords: Hippocampus comes, proximate analysis, amino acid, and bioactive compounds

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Comparative Analysis of the Nutritive Value of Smoked, Dried Procambarus clarkii (Freshwater Crayfish) from Three Nigerian States

Asian Journal of Fisheries and Aquatic Research ◽

10.9734/ajfar/2021/v11i330206 ◽

2021 ◽

pp. 39-45

Author(s):

Oluwafemi Akinsola Omole ◽

Oluwafunmike Blessing Adepoju ◽

Oluwasola Toluwalope Moradeyo ◽

Olayinka Josephine Okuneye ◽

Adunola Abosede Bello

Keyword(s):

Amino Acids ◽

Amino Acid ◽

Proximate Composition ◽

Nutritive Value ◽

Procambarus Clarkii ◽

Essential Amino Acids ◽

Freshwater Crayfish ◽

Crude Fibre ◽

Mineral Contents ◽

Significant Difference

The nutritive value of smoked, dried Procambarus clarkii sourced from Akwa Ibom, Rivers and Lagos states of Nigeria were evaluated and compared. The proximate composition of the crayfish samples were determined using official methods of analysis, mineral composition were determined using Atomic Absorption Spectrophotometer and the amino acid composition were analysed using Amino Acid Analyser. The proximate composition of the crayfish samples from three different locations (Akwa Ibom, Lagos and Rivers states) such as moisture, crude protein, crude fat and ash contents showed significant difference (p<0.05) across the selected locations while crude fibre and carbohydrate content showed no significant difference (p<0.05) across the selected locations. The amino acids composition gave the crayfish sample from Akwa Ibom state as the richest source of essential amino acids. The mineral contents of the crayfish samples such as potassium, phosphorus, magnesium, manganese and calcium showed significant difference (p<0.05) across the selected locations while calcium, zinc and sodium contents showed no significant difference (p<0.05) across the selected locations. Comparing their nutrient component, smoked, dried P. Clarkia from Awka Ibom state possess the richest nutritive value.

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Proximate Composition, Amino Acid Profile, and Mineral Content of Four Sheep Meats Reared Extensively in Morocco: A Comparative Study

The Scientific World JOURNAL ◽

10.1155/2021/6633774 ◽

2021 ◽

Vol 2021 ◽

pp. 1-11

Author(s):

Kamal Belhaj ◽

Farid Mansouri ◽

Abdessamad Ben moumen ◽

Marianne Sindic ◽

Marie-Laure Fauconnier ◽

...

Keyword(s):

Amino Acids ◽

Amino Acid ◽

Proximate Composition ◽

Mineral Content ◽

Essential Amino Acids ◽

Amino Acid Profile ◽

Point Of View ◽

Iron And Zinc ◽

Longissimus Lumborum ◽

Phosphorus And Potassium

This study aimed to compare the organoleptic and nutritional quality of four sheep meats produced in Morocco. This comparison was carried out by analyzing the proximate composition, amino acid profile, and mineral content of meat. The majority of the evaluated parameters were influenced by genetic and geographical factors ( p < 0.05 ). The longissimus lumborum muscle had higher a ∗ value in Timahdite and Ouled-Djellal breeds. The highest values of macroelement were recorded in samples of Beni-Guil meat sampled in the Tendrera region (BGT; 1067.3 mg/100 g), while the highest microelement content was registered in Timahdite meat (5.7 mg/100 g). Iron and zinc were the major identified trace elements, while phosphorus and potassium were the most abundant macroelements. The abundant amino acid was glutamic, while cysteine and methionine were least abundant. The higher essential amino acids index (IEAA) was recorded in the Timahdite sheep meat (162.20, 158.71, 161.02, and 159.65, respectively, for Timahdite, BGT, Ouled-Djellal breeds, and Beni-Guil meat sampled in Ain Beni Mathar region). From a nutritional point of view, the studied meats had a good protein and mineral quality, due to their richness in essential amino acids and microelements. The present study provided new insights on the organoleptic quality and the nutritional value of three Moroccan sheep meats reared in outdoor production system.

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Determination of proximate composition, amino acids, minerals and phytochemical profile of Cassava (Manihot esculenta) peel from sweet cassava variety grown in Yobe State of North Eastern Nigeria

Nigerian Journal of Animal Production ◽

10.51791/njap.v48i1.2894 ◽

2021 ◽

Vol 48 (1) ◽

pp. 124-134

Author(s):

I. B. Amaza

Keyword(s):

Amino Acids ◽

Amino Acid ◽

Proximate Composition ◽

Amino Acid Content ◽

Essential Amino Acids ◽

Metabolizable Energy ◽

Phytochemical Content ◽

Nitrogen Free Extract ◽

Acides Aminés ◽

Cassava Peel

The proximate composition, amino acid, mineral, and Phytochemical content of Cassava peel was determined. The peels were found to contain 8.70 % moisture, 4.89 % crude protein, 8.75 % crude fiber, 2.15 % crude fat, 8.93 % ash. Nitrogen free extract and metabolizable energy values were 66.56 % and 2717.96kcal, respectively. The results of amino acid content revealed that the histidine and tyrosine are the most limiting amino acid with values of 0.005% each. Essential amino acids such as lysine, leucine, methionine and Isoleucine with values of 0.032, 0.067, 0.076 and 0.070% were low. Mineral profile revealed that magnesium (913.74mg/kg) and iron (111.50mg/kg) as the most abundant mineral. Concentrations of phytochemicals were 26.06, 699.8, 0.063, 24.81,8.37,1457mg/100g and 2.84 for saponin, tannin, hydrogen cyanide, phytate, trypsin inhibitors, alkaloid and oxalate respectively. Based on results of this study, cassava peel can be potential source of energy; however, protein and amino acids contents are very low. It is therefore, recommended that feeding of animal with cassava peel based diets be supplemented with good quality amino acids. La composition immédiate, l'acide aminé, la teneur minérale et phytochimique de la peau de manioc ont été déterminées. Les pelures contenaient 8,70 % d'humidité, 4,89 % de protéines brutes, 8,75 % de fibres brutes, 2,15 % de matières grasses brutes, 8,93 % de cendres. L'extrait sans azote et les valeurs énergétiques métabolisables étaient respectivement de 66,56 % et 2 717,96 kcal. Les résultats de la teneur en acides aminés ont révélé que l'histidine et la tyrosine sont l'acide aminé le plus limitant avec des valeurs de 0,005% chacune. Les acides aminés essentiels tels que la lysine, la leucine, la méthionine et l'isoleucine avec des valeurs de 0,032, 0,067, 0,076 et 0,070% étaient faibles. Le profil minéral a indiqué que le magnésium (913.74mg/kg) et le fer (111.50mg/kg) sont des minéraux les plus abondants. Les concentrations de produits phytochimiques étaient de 26,06, 699,8, 0,063, 24,81,8,37,1457mg/100g et 2,84 pour la saponine, le tanin, le cyanure d'hydrogène, le phytate, les inhibiteurs de la trypsine, l'alcaloïde et l'oxalate respectivement. D'après les résultats de cette étude, la peau de manioc peut être une source potentielle d'énergie ; cependant, la teneur en protéines et en acides aminés est très faible. Il est donc recommandé que l'alimentation des animaux avec des régimes à base de pelures de manioc soit complétée par des acides aminés de bonne qualité.

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Screening of amino acid constituents from date palm fruits

International Journal of Bioassays ◽

10.21746/ijbio.2016.10.0011 ◽

2016 ◽

Vol 5 (10) ◽

pp. 4972

Author(s):

Lata Birlangi

Keyword(s):

Amino Acids ◽

Amino Acid ◽

United Arab Emirates ◽

Nutritional Value ◽

Date Palm ◽

Amino Acid Content ◽

Essential Amino Acids ◽

Cultivated Plants ◽

Palm Fruits ◽

Social Part

The date palm (Phoenix dactylifera L.) is one of mankind’s oldest cultivated plants. The fruit of the date palm is an important crop of the hot arid and semi-arid regions of the world. It has always played a genuine economic and social part in the lives of the people of these areas. The present objective in examining the amino acid content of different varieties of date palm fruits from Middle-East region; is to determine whether its protein could effectively supplement the nutritional value and it is also aimed in finding which variety is rich in number of amino acids. The phytochemical screening revealed the presence of eight essential amino acids and five non-essential amino acids in the date fruits. Among all the date fruit varieties taken as samples for the study, Dabbas cultivar of United Arab Emirates found to exhibit eight types of amino acids which includes five as non-essential ones. Total of thirteen amino acids were detected in the seven date cultivars. Determination of amino acid can serve as a guide to the possible nutritional value.

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Progress towards reduced-crude protein diets for broiler chickens and sustainable chicken-meat production

Journal of Animal Science and Biotechnology ◽

10.1186/s40104-021-00550-w ◽

2021 ◽

Vol 12 (1) ◽

Author(s):

Sonia Yun Liu ◽

Shemil P. Macelline ◽

Peter V. Chrystal ◽

Peter H. Selle

Keyword(s):

Amino Acids ◽

Amino Acid ◽

Broiler Chickens ◽

Crude Protein ◽

Plasma Concentrations ◽

Essential Amino Acids ◽

Chicken Meat ◽

Meat Production ◽

Accurate Identification ◽

Reduced Crude Protein

AbstractThe prime purpose of this review is to explore the pathways whereby progress towards reduced-crude protein (CP) diets and sustainable chicken-meat production may be best achieved. Reduced-CP broiler diets have the potential to attenuate environmental pollution from nitrogen and ammonia emissions; moreover, they have the capacity to diminish the global chicken-meat industry’s dependence on soybean meal to tangible extents. The variable impacts of reduced-CP broiler diets on apparent amino acid digestibility coefficients are addressed. The more accurate identification of amino acid requirements for broiler chickens offered reduced-CP diets is essential as this would diminish amino acid imbalances and the deamination of surplus amino acids. Deamination of amino acids increases the synthesis and excretion of uric acid for which there is a requirement for glycine, this emphasises the value of so-called “non-essential” amino acids. Starch digestive dynamics and their possible impact of glucose on pancreatic secretions of insulin are discussed, although the functions of insulin in avian species require clarification. Maize is probably a superior feed grain to wheat as the basis of reduced-CP diets; if so, the identification of the underlying reasons for this difference should be instructive. Moderating increases in starch concentrations and condensing dietary starch:protein ratios in reduced-CP diets may prove to be advantageous as expanding ratios appear to be aligned to inferior broiler performance. Threonine is specifically examined because elevated free threonine plasma concentrations in birds offered reduced-CP diets may be indicative of compromised performance. If progress in these directions can be realised, then the prospects of reduced-CP diets contributing to sustainable chicken-meat production are promising.

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Further evidence that mechanisms of host/symbiont integration are dissimilar in the maternal versus embryonic Acyrthosiphon pisum bacteriome

EvoDevo ◽

10.1186/s13227-020-00168-5 ◽

2020 ◽

Vol 11 (1) ◽

Author(s):

Celeste R. Banfill ◽

Alex C. C. Wilson ◽

Hsiao-ling Lu

Keyword(s):

Amino Acids ◽

Amino Acid ◽

Acyrthosiphon Pisum ◽

Essential Amino Acids ◽

Developmental Time ◽

Follicular Epithelium ◽

Future Research ◽

Buchnera Aphidicola ◽

Bacterial Endosymbionts ◽

Asexual Embryogenesis

Abstract Background Host/symbiont integration is a signature of evolutionarily ancient, obligate endosymbioses. However, little is known about the cellular and developmental mechanisms of host/symbiont integration at the molecular level. Many insects possess obligate bacterial endosymbionts that provide essential nutrients. To advance understanding of the developmental and metabolic integration of hosts and endosymbionts, we track the localization of a non-essential amino acid transporter, ApNEAAT1, across asexual embryogenesis in the aphid, Acyrthosiphon pisum. Previous work in adult bacteriomes revealed that ApNEAAT1 functions to exchange non-essential amino acids at the A. pisum/Buchnera aphidicola symbiotic interface. Driven by amino acid concentration gradients, ApNEAAT1 moves proline, serine, and alanine from A. pisum to Buchnera and cysteine from Buchnera to A. pisum. Here, we test the hypothesis that ApNEAAT1 is localized to the symbiotic interface during asexual embryogenesis. Results During A. pisum asexual embryogenesis, ApNEAAT1 does not localize to the symbiotic interface. We observed ApNEAAT1 localization to the maternal follicular epithelium, the germline, and, in late-stage embryos, to anterior neural structures and insect immune cells (hemocytes). We predict that ApNEAAT1 provisions non-essential amino acids to developing oocytes and embryos, as well as to the brain and related neural structures. Additionally, ApNEAAT1 may perform roles related to host immunity. Conclusions Our work provides further evidence that the embryonic and adult bacteriomes of asexual A. pisum are not equivalent. Future research is needed to elucidate the developmental time point at which the bacteriome reaches maturity.

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