scholarly journals Anti-inflammatory and Analgesic Activities of the Methanolic Extract and the Residual Fraction of the Stem Bark of Daniellia oliveri (Fabaceae)

2021 ◽  
pp. 104-111
Author(s):  
Mariam Traore ◽  
Adjaratou C. Coulibaly ◽  
Kadiatou T. Traore ◽  
Abdoul G. L. Boly ◽  
Esther W. L. M. B. Kabre ◽  
...  
Keyword(s):  
Analgesic Effect ◽  
Methanolic Extract ◽  
Lethal Dose ◽  
Clinical Signs ◽  
Residual Fraction ◽  
Dependent Manner ◽  
Oral Toxicity ◽  
Anti Inflammatory ◽  
Analgesic Activities ◽  
Daniellia Oliveri

Aims: The aim of this study was to evaluate the anti-inflammatory and analgesic activities of Daniellia oliveri methanolic extract and its fractions in NMRI mice. Study Design: In vivo acute toxicity, anti-inflammatory and analgesic assays. Place and Duration of Study: The work was carried out in the Department of Traditional Pharmacopoeia and Pharmacy (MEPHATRA / PH) of the Research Institute for Health Sciences (IRSS) Ouagadougou (Burkina- Faso) between December 2020 and February 2021. Methodology: The toxicity of the extracts was assessed according to OECD guideline 423 of 2001 at a single dose of 2000 mg / kg body weight. Analgesic effect was evaluated on the number of abdominal contortions induced by the intraperitoneal injection of acetic acid and the anti-inflammatory activity using the Carrageenan anti-edematous test was determined according to Winter. Results: The results of the acute oral toxicity study in mice showed no clinical signs of toxicity at dose of 2000 mg/kg b.w. The lethal dose (LD50) value estimated to 5000 mg/kg. The extracts reduced edema from the first hour, then by the third hour and maximum inhibition was achieved by the fifth hour after the injection of carrageenan. Extract and methanolic fraction at different doses showed significant inhibition of abdominal contortions in mice in a dose dependent manner. At 200mg the analgesic effect of methanolic fraction and crude extract was 53.70±1.29% and 41.38±1.25% respectively. At 400 mg/kg, the methanolic fraction inhibited carragenaan-induced edema by 85.97±5.67%. Conclusion: Daniellia oliveri is an important source of anti-inflammatory and analgesic compounds, justifying the use of this plant in traditional medicine for the treatment of inflammatory diseases.

Antioxidant and Anti-inflammatory Activities of Organic and Aqueous Extracts of Northeast Algerian Marrubium vulgare

2018 ◽  
Vol 16 (S1) ◽  
pp. S119-S129
Author(s):  
I. Namoune ◽  
B. Khettal ◽  
A.M. Assaf ◽  
S. Elhayek ◽  
L. Arrar
Keyword(s):  
Ethyl Acetate ◽  
Methanolic Extract ◽  
Ethyl Acetate Extract ◽  
Interleukin 1 ◽  
Total Phenolic ◽  
Dependent Manner ◽  
Aqueous Extracts ◽  
Traditional Use ◽  
Marrubium Vulgare ◽  
Anti Inflammatory

Marrubium vulgare (Lamiaceae) is frequently used in traditional medicine to treat many illnesses from ancient times. Its beneficial effects include antibacterial, antioedematogenic, and analgesic activities. This study was designed to evaluate the antioxidant and anti-inflammatory activities of organic and aqueous extracts of the leaves, the flowers, the stems, and the roots of Marrubium vulgare. The total phenolic and flavonoid contents as well as the antioxidant and the anti-inflammatory effects of methanol, chloroform, ethyl acetate, and aqueous extracts have been investigated by using different in-vitro methods. It was found that the ethyl acetate extract from Marrubium vulgare stems had the highest total phenolic content, while the ethyl acetate extract from the leaves yielded a high concentration of flavonoids. The ethyl acetate extract from the stems exhibited the highest activity in scavenging of 2,2-diphenyl- 1-picrylhydrazyl (DPPH), as well as in protecting erythrocytes. The leaves aqueous extract exhibited the highest ferrous chelating activity and its methanolic extract was found to be the strongest inhibitor of lipid peroxidation in β-carotene bleaching assay. The leaves chloroform extracts as well as the flowers methanol, chloroform, and ethyl acetate extracts were found to decrease the pro-inflammatory tumor necrosis factor alpha (TNF-α) cytokine levels in a dose-dependent manner. On the other hand, the flowers methanolic extract and the leaves methanol, ethyl acetate, and aqueous extracts decreased the interleukin-1 beta (IL- 1β) release. It was also found that the methanol extract from the flowers and the chloroform extract from the stems of Marrubium vulgare inhibited interleukin-8 (IL-8) release. This study provides a scientific basis for the traditional use of Marrubium vulgare as an anti-inflammatory agent and for the plant to be considered as an important resource of natural antioxidants.

scholarly journals Evaluation of Dimer of Epicatechin from an Endophytic Fungus Curvularia australiensis FC2AP on Acute Toxicity Levels, Anti-Inflammatory and Anti-Cervical Cancer Activity in Animal Models

Molecules ◽  
2021 ◽  
Vol 26 (3) ◽  
pp. 654
Author(s):  
Vellingiri Manon Mani ◽  
Arockiam Jeyasundar Parimala Gnana Soundari ◽  
Balamuralikrishnan Balasubramanian ◽  
Sungkwon Park ◽  
Utthapon Issara ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Acute Toxicity ◽  
Endophytic Fungus ◽  
Lethal Dose ◽  
Chemotherapeutic Agents ◽  
Cancer Drug ◽  
Dependent Manner ◽  
Anti Cancer ◽  
Albino Mice ◽  
Anti Inflammatory

Cervical cancer, as the most frequent cancer in women globally and accounts almost 14% in India. It can be prevented or treated with vaccines, radiation, chemotherapy, and brachytherapy. The chemotherapeutic agents cause adverse post effects by the destruction of the neighboring normal cells or altering the properties of the cells. In order to reduce the severity of the side effects caused by the chemically synthesized therapeutic agents, the current research developed an anti-cancer agent dimer of epicatechin (DoE), a natural bioactive secondary metabolite (BSM) mediated from an endophytic fungus Curvularia australiensis FC2AP. The investigation has initiated with the evaluation of inhibiting the angiogenesis which is a main activity in metastasis, and it was assessed through Hen’s Egg Test on Chorio Allantoic Membrane (HET-CAM) test; the BSM inhibited the growth of blood vessels in the developing chick embryo. Further the DoE was evaluated for its acute toxicity levels in albino mice, whereas the survival dose was found to be 1250 mg/kg and the lethal dose was 1500 mg/kg body weight of albino mice; hematological, biochemical, and histopathological analyses were assessed. The anti-inflammatory responses of the DoE were evaluated in carrageenan induced Wistar rats and the reduction of inflammation occurred in a dose-dependent manner. By fixing the effective dose for anti-inflammation analysis, the DoE was taken for the anti-cervical cancer analysis in benzo (a) pyrene induced female Sprague-Dawley rats for 60 days trial. After the stipulated days, the rats were taken for hematological antioxidants, lipid peroxidation (LPO), member bound enzymes, cervical histopathological and carcinogenic markers analyses. The results specified that the DoE has the capability of reducing the tumor in an efficient way. This is the first report of flavonoid-DoE production from an endophytic fungus C. australiensis has the anticancer potentiality and it can be stated as anti-cancer drug.

In vitro antihemolytic effect, in vivo anti inflammatory and In vitro antioxidant activity of Anchusa azurea Mill

2021 ◽  
Vol 20 ◽  
Author(s):  
Boussoualim Naouel ◽  
Trabsa Hayat ◽  
Krache Imane ◽  
Ouhida Soraya ◽  
Arrar Lekhmissi ◽  
...  
Keyword(s):  
Methanolic Extract ◽  
Folk Medicine ◽  
Oxidative Degradation ◽  
Negative Control ◽  
Dependent Manner ◽  
Anti Inflammatory ◽  
Β Carotene ◽  
Inflammatory Effect

Background: Anchusa azurea Mill. (AA) is a medicinal plant largely used traditionally in folk medicine in Algeria, it is locally named: hamham. It is effective in the treatment of various diseases. Objectives: The aim of the present study is to determine the antioxidant, anti-inflammatory and anti-hemolytic effects of phenolic fractions from Anchusa azurea Mill. Methods: In this study, various extracts from Anchusa azurea Mill. (AA) using solvents with increasing polarity were prepared. The quantification of polyphenols and flavonoids was determined. The anti-radical activity of the different extracts was evaluated using DPPH and by measuring the inhibition of the oxidative degradation of β-carotene. The In vitro antihemolytic effect of the plant extracts is determined (CrE, ChE, AcE and AqE). For each extract, four concentrations were tested: 10.59, 21.18, 42.37, 84.74 µg/ml. Vitamin C is used as a standard. Free-radical attack was measured by measuring the HT50 (Half-Hemolysis Time). The anti-inflammatory effect using PMA on mice of the methanolic extract (CrE) was evaluated. Results: The quantification of polyphenols and flavonoids showed that ethyl acetate extract (AcE) contains a higher amount of polyphenols. However, chloroform extract (ChE) presents a higher amount of flavonoids. AcE showed an important scavenging activity using the DPPH radical (IC50= 68.35 µg/ml). The results showed that AcE also exhibited very great inhibition on the oxidation of β-carotene/linoleic acid (84.33%). All extracts increased the HT50 values (Half-Hemolysis Time) in a dose-dependent manner. The three highest concentrations (21.18, 42.37 and 84.74 µg / ml) of ChE caused a very significant delay (p ≤ 0.001) of hemolysis compared to the negative control and the positive control "VIT C". The anti-inflammatory effect using PMA on mice showed that the methanolic extract (CrE) of AA reduced the weight of the ear edema. Conclusions: This plant has a strong pharmacological power, which supports its traditional medicinal use.

scholarly journals INVESTIGATING THE ROLE OF NF-κB, COX-1, COX-2, COMT, IL-10, IL-6 AND TNF-α IN MODULATING ANTI-NOCICEPTIVE ACTIVITY OF METHANOLIC EXTRACT OF ENTADA PHASEOLOIDES

2020 ◽  
pp. 172-179
Author(s):  
CHANDANA CHOUDHURY BARUA ◽  
S. M. ABDUL AZIZ BARBHUIYA ◽  
LIPIKA BURAGOHAIN ◽  
AJAY KAKATI ◽  
ACHEENTA GOHAIN BARUA
Keyword(s):  
Methanolic Extract ◽  
Water Immersion ◽  
Hot Water ◽  
Dependent Manner ◽  
Hot Plate ◽  
Writhing Test ◽  
Tnf Α ◽  
Entada Phaseoloides ◽  
Anti Inflammatory ◽  
Cox 1

Objective: To investigate the analgesic activity of methanolic extract of Entada phaseoloides (MEEP) along with its molecular mechanistic pathway. Methods: Swiss albino female mice and Wistar rats of either sex were administered orally with MEEP extracts (100, 200 and 400 mg/kg) and pentazocine, tramadol and diclofenac sodium, as standard drugs. Following administration, anti-nociceptive activity was evaluated using an acetic acid-induced writhing test, Eddy’s hot plate, and hot water immersion test. Serum was collected for molecular expression of various proteins and genes using Reverse Transcriptase PCR and Western Blotting. Results: Acetic acid writhing test, a frequently used method to assess peripheral analgesic activity, revealed that MEEP reduced peripherally induced pain in a dose-dependent manner. Likewise, Eddy’s hot plate and hot water immersion methods, often implicated for testing central analgesic activities, showed that MEEP is bestowed with the capability to counteract analgesia in a dose and time-dependent manner. Pro-inflammatory cytokines and factors like COX-2, IL-6, TNF-α, and NF-κB that cause inflammatory responses and pain were significantly reduced, suggesting its analgesic and anti-inflammatory potential. This analgesic and the anti-inflammatory role played by MEEP is also supported by the up-regulation of anti-inflammatory cytokine IL-10 and COMT and COX-1 enzyme demonstrated no significant difference between the groups. Conclusion: The study revealed the weak peripheral and potent central analgesic property MEEP by modulating pro-inflammatory and anti-inflammatory pathways.

scholarly journals Acute and Subacute Oral Toxicity of Mumefural, Bioactive Compound Derived from Processed Fruit of Prunus mume Sieb. et Zucc., in ICR Mice

Nutrients ◽  
2020 ◽  
Vol 12 (5) ◽  
pp. 1328
Author(s):  
Jungim Kim ◽  
Mira Han ◽  
Won Kyung Jeon
Keyword(s):  
Hematological Parameters ◽  
Lethal Dose ◽  
Clinical Signs ◽  
Bioactive Compound ◽  
Toxicity Assessment ◽  
Prunus Mume ◽  
Male Mice ◽  
Health Food ◽  
Oral Toxicity ◽  
Icr Mice

Mumefural is a bioactive compound derived from the processed fruit of Prunus mume Sieb. et Zucc., a traditional health food; however, its safety has not been evaluated. We investigated the toxicity of mumefural through single and repeated oral administration at doses of 1250, 2500, and 5000 mg/kg in Institute of Cancer Research (ICR) mice. The acute toxicity assessment was not associated with adverse effects or death. Similarly, the subacute (four weeks) toxicity assessment did not reveal any mumefural-associated mortality, abnormal organ damage, or altered clinical signs, body weight, food consumption, or hematological parameters. However, albumin/globulin ratio and chloride ion levels were significantly increased in male mice treated with mumefural at ≥2500 mg/kg. Female mice exhibited significantly higher levels of chloride, sodium, and potassium ions, at a dose of 5000 mg/kg. Furthermore, the administration of 2500 and 5000 mg/kg mumefural decreased the absolute weight of spleen in male mice. These findings indicated that the approximate lethal dose of mumefural in ICR mice was >5000 mg/kg. No significant mumefural toxicity was observed at ≤5000 mg/kg. Our findings provide a basis for conducting future detailed studies to evaluate reproductive, neurological, genetic, and chronic toxicity of mumefural.

scholarly journals Anti-inflammatory and antinociceptive activities of methanolic extract from red seaweed Dichotomaria obtusata

2013 ◽  
Vol 49 (1) ◽  
pp. 65-74 ◽  
Author(s):  
Neivys García Delgado ◽  
Ana Iris Frías Vázquez ◽  
Hiran Cabrera Sánchez ◽  
Roberto Menéndez Soto del Valle ◽  
Yusvel Sierra Gómez ◽  
...  
Keyword(s):  
Phospholipase A2 ◽  
Methanolic Extract ◽  
Therapeutic Potential ◽  
Intraperitoneal Administration ◽  
Moderate Activity ◽  
Dependent Manner ◽  
Ear Edema ◽  
Antinociceptive Effects ◽  
Anti Inflammatory ◽  
Phospholipase A2 Activity

The aim of the present work was to investigate the anti-inflammatory and antinociceptive effects of methanolic extract from D. obtusata using classic models in mice (croton oil-induced ear edema and acetic acid-induced writhing) and a phospholipase A2 activity test. Qualitative analysis of the chemical composition of seaweed was also determined by extraction with solvents of increasing polarity and precipitation and color tests. Results of qualitative chemical study showed the presence of lactonic and phenolic compounds, reduced carbohydrates, other sugars, flavonoids, fatty compounds, triterpenes and steroids. The extract inhibited mouse ear edema in a dose-dependent manner with an efficacy higher than 90% and a mean effective dose of 4.87µg/ear, while intraperitoneal administration presented a moderate activity. The extract did not inhibit phospholipase A2 activity. In the writhing test, the intraperitoneal administration of the extract showed a strong antinociceptive activity (80.2%), while the oral route showed a lower efficacy. In conclusion, this study demonstrated the anti-inflammatory and antinociceptive effects of methanol extract of D. obtusata in experimental models, suggesting its therapeutic potential in the treatment of peripheral painful and/or inflammatory pathologies.

scholarly journals ANALGESIC EFFECT AND ANTI-INFLAMMATORY ACTIVITY OF AQUEOUS EXTRACT OF BOSWELLIA DALZIELII (BURSERACEAE) STEM BARK

2018 ◽  
Vol 10 (4) ◽  
pp. 139
Author(s):  
Iyabo M. ◽  
Adebisi . ◽  
Abba Abubakar ◽  
Kabiru Abubakar ◽  
Tijjani R. ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Aqueous Extract ◽  
Analgesic Effect ◽  
Late Phase ◽  
Significant Activity ◽  
Oral Toxicity ◽  
Standard Drug ◽  
Writhing Test ◽  
Paw Oedema ◽  
Anti Inflammatory

Objective: To evaluate the analgesic effect and anti-inflammatory properties of Boswellia dalzielii (Burseraceae), a medicinal plant commonly used in northern Nigeria as an anti-diarrhoeal, antipyretic, analgesic and anti-inflammatory agent.Methods: Three doses (25 mg/kg, 50 mg/kg and 100 mg/kg) of the crude aqueous extract of Boswellia dalzielii were evaluated for analgesic and anti-inflammatory activities using the acetic acid-induced writhing test, formalin-induced nociception and formalin-induced hind paw oedema in rats. The acute oral toxicity was carried out using the up and down procedure as described by the OECD guidelines.Results: All doses (25, 50 and 100 mg/kg) of the extract tested were effective against acetic acid induced abdominal constrictions producing a percentage inhibition of (55.43, 69.56 and 71.73%) respectively. A percentage inhibition of the formalin-induced nociception of (7.31, 31.70 and 48.78%-early phase) and (12.82, 21.79 and 48.71%-late phase) respectively was also produced. For the acetic acid writhing test, the percentage inhibition obtained at the dose of 50 and 100 mg/kg (69.56 and 71.73%) were higher than that of the standard drug (Piroxicam, 10 mg/kg) (59.78%). For formalin-induced nociception, the test extract at 100 mg/kg showed a higher percentage inhibition compared to Piroxicam, in early (48.78 and 43.90%) and late phase (48.71 and 39.74 %) respectively. The extract, however, did not show a significant activity against formalin-induced paw oedema at all the doses used.Conclusion: The present study demonstrated that Boswellia dalzielii has significant analgesic properties comparable to that of the standard drug (10% Piroxicam), thus validating the traditional claim of its antinociceptive property.

Methyl Gallate Inhibits the Production of Interleukin-6 and Nitric Oxide via Down-Regulation of Extracellular-Signal Regulated Protein Kinase in RAW 264.7 Cells

2010 ◽  
Vol 38 (05) ◽  
pp. 973-983 ◽  
Author(s):  
Hee-Sung Chae ◽  
Ok-Hwa Kang ◽  
Jang-Gi Choi ◽  
You-Chang Oh ◽  
Young-Seob Lee ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Raw 264.7 Cells ◽  
Raw 264.7 ◽  
Dependent Manner ◽  
Methyl Gallate ◽  
Extracellular Signal ◽  
Anti Inflammatory ◽  
Analgesic Activities ◽  
Oxide Synthase

To determine the anti-inflammatory and analgesic activities of methyl gallate (MG) isolated from Galla Rhois, MG was studied in vivo for its analgesic activities using the writhing response in mice. Anti-inflammatory activity of MG was evaluated for NO and IL-6 production in RAW 264.7 cells. MG inhibited LPS-induced NO and IL-6 production. Consistent with these observations, the protein and mRNA expressions of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) were inhibited by MG. Moreover, MG suppressed the phosphorylation of ERK1/2 in LPS-induced RAW 264.7 cells in a dose-dependent manner. Taken together, the results of this study indicate that MG has anti-inflammatory effects.

scholarly journals Antinociceptive and Anti-inflammatory Effects of Methanolic Extract of Laurencia caspica

2020 ◽  
Vol 5 (2) ◽  
pp. 54-60
Author(s):  
Katayoon Karimzadeh ◽  
Mahdiyeh Ramzanpoor ◽  
Shadi Keihankhadiv
Keyword(s):  
Acetic Acid ◽  
Analgesic Effect ◽  
Pain Treatment ◽  
Methanolic Extract ◽  
Inflammatory Activity ◽  
Paw Edema ◽  
Hot Plate ◽  
Hot Plate Test ◽  
Second Phases ◽  
Anti Inflammatory

Introduction: Seaweeds are valuable resources for the discovery of efficient and safe drugs for pain treatment. In the present investigation, we evaluated the antinociceptive and anti-inflammatory properties of, methanolic extract of Laurencia caspica, a red algae, in mice models. Methods: The analgesic effect of methanolic extract of L. caspica was assessed by hot-plate and acetic acid-induced writhing tests in male Swiss albino mice (weight = 20-25 g). The anti-inflammatory activity of methanolic extract of L. caspica was also evaluated by formalin-induced ear edema and xylene-induced paw edema tests. Results: The total flavonoid content of the extract was estimated as 0.0537 mg quercetin/g extract. Both first and second phases of the nociception were significantly inhibited at a dose of 120 mg/kg of methanolic extract of L. caspica. The observed anti-inflammatory effect was dose-dependent. Acetic acid-induced writhing test and hot plate test showed that the extract significantly reduced pain in all evaluated doses (15, 30, 60, and 120 mg/kg). The antinociceptive activity of the methanolic extract was significantly reduced by naloxone (4 mg/kg). Moreover, the extract significantly reduced paw edema at the dose of 120 mg/kg in all the animals. Conclusion: Methanolic extract of L. caspica exhibited central analgesic effect, as well as anti-inflammatory activity probably due to the presence of constituents like flavonoids and triterpenoids.

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