scholarly journals Investigation of the Phytochemistry and Antioxidant Activity of Pterocarpus angolensis (Mubvamaropa) from Mupandawana

2021 ◽  
pp. 29-36
Author(s):  
S. Zengeni ◽  
J. M. Chifamba ◽  
I. Mutingwende ◽  
T. Manyarara
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Water Extract ◽  
Ethanolic Extract ◽  
Bark Extract ◽  
Phytochemical Analysis ◽  
Constant Weight ◽  
Medicinal Uses ◽  
Pterocarpus Angolensis ◽  
Dpph Scavenging

The present study investigated the phytochemistry and antioxidant activity of Pterocarpus angolensis (Mubvamaropa) from Mupandawana. Pt. angolensis has numerous medicinal uses. The bark, sap, leaves and roots are all used to treat different ailments. The sap is used to stop nose bleeds, treatment of ulcers and kill ringworms. Several studies have supported the use of the tree’s sap to treat cataracts, malaria as well as skin inflammations. Pterocarpus angolensis bark was collected from Gutu, Masvingo province and authenticated by a plant taxonomist at the Zimbabwe National herbarium (Mr Chapano). The bark was cleaned using distilled water and oven dried at 40°C for 4 hours to constant weight. Size reduction was achieved using a Thomas-Wiley laboratory mill model with a 2 mm sieve mesh. The phytochemical analysis revealed that the ethanolic extract had more phyto-constituents than the water extract which only four phyto-constituents present all of which were weakly positive. DPPH scavenging activity was 95.11% at a concentration of 500 μg/mL bark extract, while that of the control, ascorbic acid, was 97.60%. Ascorbic acid had an IC50 value of 4.35 µg/mL while the bark extract had a value of 150.64 µg/mL.

scholarly journals Antioxidant Activity of the Extracts Derived from Terminalia catappa

2011 ◽  
Vol 54 (2) ◽  
pp. 93-98
Author(s):  
Rahmanullah Siddiqi ◽  
Syed Asad Sayeed ◽  
Shahina Naz ◽  
Syed Muhammad Ghufran Saeed
Keyword(s):  
Antioxidant Activity ◽  
Peroxide Value ◽  
Corn Oil ◽  
Ethanolic Extract ◽  
Phytochemical Analysis ◽  
Peroxide Formation ◽  
Aqueous Fraction ◽  
Scavenging Effect ◽  
Terminalia Catappa ◽  
Dpph Scavenging

The extracts derived from Terminalia catappa leaves and fruit following antioxidant activity directed isolation, were screened for their antioxidant activity through their ability to scavenge DPPH radicals. Only fractions which exhibited >50% DPPH scavenging effect at each step of isolation were selected for further purification and judge their ability to reduce peroxide formation (peroxide value) in heated corn oil. The results indicated that crude ethanolic extract, aqueous fraction of crude extract and its sub fractions (petroleum ether and ethylacetate) possessed prominent antioxidant activity. In addition, phytochemical analysis showed that the five fractions obtained finally contain simple phenols, anthocyanins, phenyl propanoids and flavanols.

scholarly journals Phytochemical analysis and Invitro antioxidant activity of silver nanoparticle synthesized Crateva religiosa bark extract

2021 ◽  
Vol 23 (05) ◽  
pp. 190-198
Author(s):  
Charu Prabha. V ◽  
◽  
Dr. K. Chandra Prabha ◽  
Dr.S.Vijaya kumar ◽  
◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Aqueous Extract ◽  
Silver Nanoparticle ◽  
Antioxidant Property ◽  
Bark Extract ◽  
Total Phenol Content ◽  
Phytochemical Analysis ◽  
Frap Assay ◽  
Ic50 Value ◽  
Dpph Scavenging

The aim of the study is to determine the phytochemical analysis and antioxidant activity of silver nanoparticle synthesized aqueous bark extract of Crateva religiosa. The aqueous extract of Crateva religiosa barks are prepared by maceration method. The qualitative phytochemical and quantitative phytochemical analysis was carried out using standard procedures. Silver nanoparticle was synthesized for the aqueous extract. Then the antioxidant activity was determined by DPPH scavenging activity and FRAP assay. The qualitative phytochemical analysis shows the presence of alkaloids, flavonoids, phenols, tannins, saponins. Total phenol content of bark extract was found to be 1.423mg/g. Total flavonoid content was found to be 1.6mg/g.UV spectrophotometry peak confirms the presence of silver nanoparticle. The extract shows effective antioxidant property with IC50 value 42.32 µg/ml for DPPH assay and IC50 value 45 µg/ml for FRAP. The Crateva religiosa bark extract could be a novel source of antioxidant.

Determination of Antioxidant Activities and Total Phenolic Contents of Three Cyanobacterial Species

2014 ◽  
Vol 625 ◽  
pp. 826-829 ◽  
Author(s):  
Hamdy Elsayed Ahmed Ali ◽  
Sanaa Mahmoud Metwally Shanab ◽  
Mervat Aly Mohamed Abo-State ◽  
Emad Ahmed Ahmed Shalaby ◽  
Usama Mohamed Nour El Demerdash ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Water Extract ◽  
Correlation Coefficients ◽  
Ethanolic Extract ◽  
Natural Antioxidants ◽  
Total Phenolic ◽  
Phenolic Contents ◽  
Cyanobacterial Species ◽  
Dpph Scavenging

The antioxidant activity and total phenolic content of different extracts (hexane, chloroform, ethyl acetate, ethanol (70%) and water) of three tested cyanobacterial species (Phormidium sp., Oscillatoria sp. and Nostoc muscorum ) were evaluated, using 2,2 ́-diphenyl-1-picrylhydrazyl (DPPH) scavenging method and Folin–Ciocalteu method, respectively. The ethanolic extract of Oscillatoria sp. possessed the highest antioxidant activity (69.13±0.35 %) and therefore could be potential source of natural antioxidants. The correlation coefficients between the antioxidant activities and the phenolic contents were very small in most of extracts (R2 = 0.0291-0.2934). However, the water extract demonstrated relatively higher value (R2= 0.7446). Thus, phenolic compounds may not be considered the major contributors for the antioxidant activities of these cyanobacterial species. So, other antioxidant substances may be present in the extract and share in this activity.

scholarly journals Phytochemical Analysis, Pharmacological and Safety Evaluations of Halophytic Plant, Salsola cyclophylla

Molecules ◽  
2021 ◽  
Vol 26 (8) ◽  
pp. 2384
Author(s):  
Hamdoon A. Mohammed ◽  
Mohsen S. Al-Omar ◽  
Salman A. A. Mohammed ◽  
Ahmad H. Alhowail ◽  
Hussein M. Eldeeb ◽  
...  
Keyword(s):  
Ethanolic Extract ◽  
Phytochemical Analysis ◽  
High Concentration ◽  
Halophytic Plant ◽  
Anti Oxidant ◽  
Aqueous Ethanolic Extract ◽  
Phytochemical Constituents ◽  
Anti Inflammatory ◽  
Dpph Scavenging ◽  
Anti Inflammation

Salsola cyclophylla, an edible halophyte, is traditionally used for inflammation and pain. To confirm the claimed anti-inflammatory and analgesic properties, a detailed study on respective pharmacological actions was undertaken. The activities are contemplated to arise from its phytoconstituents. The LC-MS analysis of S. cyclophylla 95% aqueous-ethanolic extract revealed the presence of 52 compounds belonging to phenols, flavonoids, coumarins, and aliphatics class. A high concentration of Mn, Fe, and Zn was detected by atomic absorption spectroscopic analysis. The ethyl acetate extract showed the highest flavonoid contents (5.94 ± 0.04 mg/g, Quercetin Equivalents) and Fe2+-chelation (52%) potential with DPPH radicals-quenching IC50 at 1.35 ± 0.16 mg/mL, while the aqueous ethanolic extract exhibited maximum phenolics contents (136.08 ± 0.12 mg/g, gallic acid equivalents) with DPPH scavenging potential at IC50 0.615 ± 0.06 mg/mL. Aqueous ethanolic extract and standard quercetin DPPH radicals scavenging’s were equal potent at 10 mg/mL concentrations. The aqueous ethanolic extract showed highest analgesic effect with pain reduction rates 89.86% (p = 0.03), 87.50% (p < 0.01), and 99.66% (p = 0.0004) after 60, 90, and 120 min, respectively. Additionally, aqueous ethanolic extract exhibited the highest anti-inflammation capacity at 41.07% (p < 0.0001), 34.51% (p < 0.0001), and 24.82% (p < 0.0001) after 2, 3, and 6 h of extract’s administration, respectively. The phytochemical constituents, significant anti-oxidant potential, remarkable analgesic, and anti-inflammatory bioactivities of extracts supported the traditionally claimed anti-inflammatory and analgesic plant activities.

Phytochemical analysis, Antimicrobial and Antioxidant assay of Bhut Jolokia pepper

2021 ◽  
pp. 3775-3778
Author(s):  
Dharani Dharan ◽  
K. Venkatesh ◽  
S.S. Meenambiga ◽  
Dhivya Dhanasekar ◽  
P. Arumugam
Keyword(s):  
Staphylococcus Aureus ◽  
Ethanolic Extract ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Phytochemical Analysis ◽  
Zone Of Inhibition ◽  
Antioxidant Assay ◽  
Dpph Scavenging ◽  
Bhut Jolokia ◽  
Antimicrobial Assay

The presented work represents phytochemical analysis, Antioxidant assay and Antimicrobial activity of Bhut jolokia pepper (Capsicum chinense Jacq) extracted by ethanol as solvent. The ethanolic extract of Bhut jolokia pepper showed the presence of terpenoids, steroids, saponins and flavonoids. Antimicrobial assay was done with varying concentration (250-1000µg/ml) of pepper extract using tetracycline as control by well diffusion method, the extract at 750µg/ml shown best inhibition zone and Staphylococcus aureus showed the highest zone of inhibition at all concentration compared to other bacteria species with maximum zone of inhibition of 27mm. The DPPH scavenging assay for antioxidant activity at 517nm showed positive activity for scavenging, scavenging increased with the concentration of extract. Thus, Bhut jolokia could be effective in treating diseases caused by Staphylococcus aureus from antimicrobial assay result.

scholarly journals Antioxidant Activity of Averrhoa bilimbi Linn. Leaves Extract Using Two Different Types of Solvents

2021 ◽  
Author(s):  
Raja Nurul Ashiqin Raja Arifin ◽  
Juliana Jumal
Keyword(s):  
Antioxidant Activity ◽  
Gallic Acid ◽  
Skin Diseases ◽  
Water Extract ◽  
Ethanolic Extract ◽  
Natural Antioxidant ◽  
Aluminium Chloride ◽  
Total Phenolic ◽  
Different Types ◽  
Gallic Acid Equivalent

Exposed to the pollution has led to generation of reactive oxygen species (ROS) in human skin. ROS generated cause many skin diseases such as skin-aging, inflammation, melanogenesis and skin cancer. ROS is a family of oxygen-based free radicals that contains or capable of producing an unpaired electron. Antioxidant is a molecule that can inhibit the reaction of free radical from ROS by donating its electron. Averrhoa bilimbi Linn. (AVBL) is one of the potent natural antioxidant belongs to the group of Oxalidaceae which can be widely found in Asia including Malaysia. Traditionally, this plant has been used to treat many diseases such as cough, itchiness, pimple, fever and inflammation. As a result, much attention has been directed towards the studies regarding the potential of this plant in treating disease. The present study was undertaken to assess the antioxidant activity of AVBL leaves extract. The AVBL leaves were extracted using sonicator with ethanol and distilled water as two different types of solvent. The total phenolic content (TPC) and flavonoid content (TFC) of this study were determined by using Folin-Ciocalteu reagent and aluminium chloride colometric assay. Antioxidant activity of the plant extract was tested using 2,2- diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing power (FRAP). From the analyses, water extract of AVBL possessed greater extraction yield (11.231%) as compared to ethanolic extract (5.358 %). However, ethanolic extract of AVBL leaves revealed higher result of TPC (126.4±0.35 mg/g gallic acid equivalent), TFC (32.80±0.37 mg/g quercetin equivalent), DPPH (0.0019±0.0003) and FRAP (41.81±0.45 mg/g gallic acid equivalent). The results of TPC and TFC have strongly positive correlation with antioxidant capacity (r = 1). Thus, it can be concluded that this plant is a potent source of natural antioxidant.

scholarly journals Antioxidant Activities of Extracts from Celery Leaves (Apium Graveolens L) Grown in Jos, Nigeria

2020 ◽  
pp. 1-5
Author(s):  
M. Suleman Stephen ◽  
E. A. Adelakun ◽  
J. H. Kanus ◽  
Meshack M. Gideon
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Acetone Extract ◽  
Apium Graveolens ◽  
Isolation And Identification ◽  
Celery Plant

The presence of natural antioxidant in plants is well known. Plant phenolics constitute one of the major groups of components that act as antioxidant and free radical terminator. Hence, this study focused on investigating the antioxidant activity of Celery plant (Apium graveolens L). The fresh leaves were collected, crushed and extracted with ethanol and acetone by maceration. The radical scavenging properties of the extracts were determined by measuring changes in absorbance of DPPH radical at a wave lenght of 517 nm by UV and ascorbic acid is used as the standard. It showed that the crude ethanolic extract has higher antioxidant activity compared to ascorbic acid and acetone extract with less scavenging activity. The values were (IC50 114.6 µg/mL) for ascorbic acid, (IC50 112 µg/mL) for the crude ethanolic extract and (IC50172 µg/mL) for crude acetone extract. The result shows that Celery plant grown in Jos possess good antioxidant properties which may be linked to the presence of phenolics and flavonoids in the plant, which justifies its use as a medicinal plant. This can be further investigated for the isolation and identification of active compounds of medicinal utilities.

scholarly journals STUDY ON MULTIPOTENT MEDICINAL ASPECTS OF SCHIMA WALLICHII (BARK) FROM NAGALAND, NE INDIA

2019 ◽  
pp. 155-158
Author(s):  
MINAKSHI BHATTACHARJEE ◽  
VITOLU SEMA Y ◽  
MANASH PRATIMSARMA
Keyword(s):  
Staphylococcus Aureus ◽  
Prothrombin Time ◽  
Standard Method ◽  
Water Extract ◽  
Ethanolic Extract ◽  
Bark Extract ◽  
Water Extracts ◽  
Ne India ◽  
Ic50 Value ◽  
Schima Wallichii

Objective: The present study focuses on the multipotent medicinal aspects of Schima wallichii bark. Methods: The ethanol and water extracts of the plant were investigated its phytochemical, antibacterial, antioxidant, and anticoagulant as per the standard method. Results: S. wallichii bark extract was effective against Staphylococcus aureus. It was also found to be a good antioxidant having an IC50 value of 98.7 in ethanolic extract. Further, the water extract increased in prothrombin time to 57.7 s against normal (12.3 s). The different phytochemicals, namely tannin, saponins, steroids, terpenoids, flavonoids, and glycosides, were present. Since the findings showed very less percent inhibition in comparison to curcumin-treated cells, thus both the extracts were both not effective in the antiproliferative assay. Conclusion: We conclude that this plant is a good candidate for developing a natural drug.

scholarly journals Antileishmanial Effect of 5,3′-Hydroxy-7,4′-dimethoxyflavanone ofPicramnia gracilisTul. (Picramniaceae) Fruit:In VitroandIn VivoStudies

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Sara M. Robledo ◽  
Wilson Cardona ◽  
Karen Ligardo ◽  
Jéssica Henao ◽  
Natalia Arbeláez ◽  
...  
Keyword(s):  
Native Species ◽  
Folk Medicine ◽  
Ethanolic Extract ◽  
Phytochemical Analysis ◽  
Medicinal Uses ◽  
Leishmanicidal Activity ◽  
Antileishmanial Drug ◽  
Low Toxicity

Species ofPicramniagenus are used in folk medicine to treat or prevent skin disorders, but only few species have been studied for biological activity and chemical composition.P. gracilisTul. is a native species from Central and South America and although its fruits are edible, phytochemical analysis or medicinal uses of this species are not known. In the search of candidates to antileishmanial drugs, this work aimed to evaluate the antileishmanial activity ofP. gracilisTul. inin vitroandin vivostudies. Only ethanolic extract of fruits showed leishmanicidal activity. The majoritarian metabolite was5,3′-hydroxy-7,4′-dimethoxyflavanoneether that exhibited high activity againstL. (V.) panamensis(EC5017.0 + 2.8 mg/mL, 53.7 μM) and low toxicity on mammalian U-937 cells, with an index of selectivity >11.8.In vivostudies showed that the flavanone administered in solution (2 mg/kg/day) or cream (2%) induces clinical improvement and no toxicity in hamsters with CL. In conclusion, this is the first report about isolation of5,3′-hydroxy-7,4′-dimethoxyflavanoneofP. gracilisTul. The leishmanicidal activity attributed to this flavanone is also reported for the first time. Finally, thein vitroandin vivoleishmanicidal activity reported here for5,3′-hydroxy-7,4′-dimethoxyflavanoneoffers a greater prospect towards antileishmanial drug discovery and development.

scholarly journals Phytochemical analysis and antioxidant activity of different plant parts of Pellacalyx axillaris

2019 ◽  
Vol 15 (3) ◽  
pp. 394-397
Author(s):  
Najwa Ahmad Kuthi ◽  
Norazah Basar
Keyword(s):  
Antioxidant Activity ◽  
Biological Activities ◽  
Radical Scavenging ◽  
Bark Extract ◽  
Phytochemical Analysis ◽  
Phytochemical Study ◽  
Antioxidant Power ◽  
Plant Parts ◽  
Crude Extracts ◽  
Radical Scavenging Assay

Pellacalyx axillaris or locally known as ‘membuloh’ is a mangrove species belonging to the Rhizophoraceae family. Till date, there has been only one phytochemical study found on this particular plant species and that without any documentation on its biological activities. Therefore, the present work aimed to reveal the phytoconstituents and the antioxidant activity of different crude extracts from different plant parts of P. axillaris. Experimentally, three organic solvents of different polarities i.e. n-hexane, ethyl acetate and methanol were used to prepare the crude extracts from the dried leaves, twigs and barks of P. axillaris. The preliminary phytochemical screening of this species indicated the presence of terpenoids, phenolic compounds, tannins, flavonoids, alkaloids, anthraquinone glycosides and carbohydrates. The in vitro antioxidant activity of the species evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay, 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radical scavenging assay, and ferric reducing antioxidant power (FRAP) suggested that the methanolic bark extract contained potential source of natural antioxidants. Further research into isolation of antioxidant compounds from this species is highly recommended.

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