scholarly journals Phytochemical analysis and Invitro antioxidant activity of silver nanoparticle synthesized Crateva religiosa bark extract

2021 ◽  
Vol 23 (05) ◽  
pp. 190-198
Author(s):  
Charu Prabha. V ◽  
◽  
Dr. K. Chandra Prabha ◽  
Dr.S.Vijaya kumar ◽  
◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Aqueous Extract ◽  
Silver Nanoparticle ◽  
Antioxidant Property ◽  
Bark Extract ◽  
Total Phenol Content ◽  
Phytochemical Analysis ◽  
Frap Assay ◽  
Ic50 Value ◽  
Dpph Scavenging

The aim of the study is to determine the phytochemical analysis and antioxidant activity of silver nanoparticle synthesized aqueous bark extract of Crateva religiosa. The aqueous extract of Crateva religiosa barks are prepared by maceration method. The qualitative phytochemical and quantitative phytochemical analysis was carried out using standard procedures. Silver nanoparticle was synthesized for the aqueous extract. Then the antioxidant activity was determined by DPPH scavenging activity and FRAP assay. The qualitative phytochemical analysis shows the presence of alkaloids, flavonoids, phenols, tannins, saponins. Total phenol content of bark extract was found to be 1.423mg/g. Total flavonoid content was found to be 1.6mg/g.UV spectrophotometry peak confirms the presence of silver nanoparticle. The extract shows effective antioxidant property with IC50 value 42.32 µg/ml for DPPH assay and IC50 value 45 µg/ml for FRAP. The Crateva religiosa bark extract could be a novel source of antioxidant.

scholarly journals Phytochemical analysis and antioxidant activity of leaf extracts of some selected plants of the family Acanthaceae

2020 ◽  
Vol 7 (2) ◽  
pp. 264
Author(s):  
Kshetrimayum Kripasana ◽  
Jobi Xavier
Keyword(s):  
Antioxidant Activity ◽  
Linear Regression Analysis ◽  
Antioxidant Property ◽  
Phytochemical Analysis ◽  
Leaf Extracts ◽  
Ic50 Value ◽  
Dpph Method ◽  
Phytochemical Compounds ◽  
Dpph Scavenging ◽  
Immense Potential

The present era of scientific research has witnessed an enumerable amount of evidences to showcase the immense potential of medicinal plants. In the present investigation, the phytochemical analysis of Phlogacanthus pubinervius T. Anderson., Adhatoda vasica (L.) Nees,Phlogacanthus thyrsiflorus Nees, Phlogacanthus curviflorus (Wall.) Nees, and Ruellia tuberosa L. was carried out for the different plants extracted with methanol. Analysis was carried out to estimate the quantity of phenols, carbohydrates, tannins, flavonoids and proteins. The antioxidant property of these plants were analysed using DPPH method. The concentration of the plant samples required to decrease the DPPH concentration by 50% was calculated by interpolation from linear regression analysis and denoted IC50 value (µg/ml). The qualitative analysis showed the presence of alkaloids, tannins, saponins, proteins, carbohydrate and phenols in all the sample extracts. The highest amount of tannins and phenols was observed in P. thyrsiflorus. P. pubinervius (77.83%), A. vasica (74.81%), P. curviflorus (94.20%), and R. tuberosa (70.78%) which showed highest antioxidant activity of DPPH-scavenging at 150 µg/ml of methanol extract. The high percent of scavenging activities of those plants add value to their medicinal properties. The presence of the high amount of phytochemical compounds suggests that the plants have high amount of medicinal compounds and can be extensively used to extract the natural compounds.

scholarly journals Phytochemical analysis and DPPH scavenging activity of Combretum punctatum var. squamosum (Combretaceae), an ethnomedicinal plant of Mizoram.

2016 ◽  
Vol 8 (3) ◽  
pp. 422
Author(s):  
Kawl Thanzami ◽  
Bibhuti Bhusan Kakoti ◽  
Christopher VL Famkima ◽  
H Lalhlenmawia
Keyword(s):  
Aqueous Extract ◽  
Phytochemical Analysis ◽  
Scavenging Activity ◽  
Flavonoid Content ◽  
Ic50 Value ◽  
Dpph Scavenging Activity ◽  
Phytochemical Constituents ◽  
Dpph Scavenging ◽  
Crude Aqueous Extract ◽  
Very High

<p>The main objective of the study is to determine the phytochemical constituents and the DPPH scavenging activity of the aqueous extract of <em>Combretum punctatum</em> var. <em>squamosum</em>.  The phytochemical screening revealed that the crude aqueous extract contained phenols, flavonoids, phytosterols and diterpenes while alkaloids, glycosides, tannins, carbohydrates, proteins and amino acids were found to be absent. The total phenol and flavonoid content in the crude aqueous extract was found to be comparatively high, i.e. 111 mg/g GAE and 120.99 mg/g QE respectively. It was also found that the % DPPH scavenging activity was very high and comparable to the standard used. The IC50 value of the aqueous extract was found to be 1.76 mg/ml which is lower but comparable to the IC50 value of the standard.</p>

EVALUATION OF ANTIOXIDANT ACTIVITY AND PHYTOCHEMICAL ANALYSIS OF HARDWIKIA BINATA (ROXB.)

2021 ◽  
pp. 256-258
Author(s):  
Varsha D. Hutke ◽  
Vaishali Rathod
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Secondary Metabolites ◽  
Aqueous Extract ◽  
Ethanol Extract ◽  
Single Species ◽  
Antioxidant Property ◽  
Phytochemical Analysis ◽  
Phytochemical Screening ◽  
The Family

The genus Hardwickia of the family Caesalpiniaceae is represented by a single species Hardwickia binata Roxb. The present study deals with screening of secondary metabolites and antioxidant activity of different extracts of leaf and bark of Hardwickia binata. Phytochemical screening revealed the presence of various secondary metabolites viz. alkaloids, saponins, phenolics compound, tannins, phenols and steroids. Both leaf and bark extracts scavenged free radicals signicantly. Whereas aqueous extract of bark and ethanol extract of leaf exhibited good antioxidant property

scholarly journals Investigation of the Phytochemistry and Antioxidant Activity of Pterocarpus angolensis (Mubvamaropa) from Mupandawana

2021 ◽  
pp. 29-36
Author(s):  
S. Zengeni ◽  
J. M. Chifamba ◽  
I. Mutingwende ◽  
T. Manyarara
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Water Extract ◽  
Ethanolic Extract ◽  
Bark Extract ◽  
Phytochemical Analysis ◽  
Constant Weight ◽  
Medicinal Uses ◽  
Pterocarpus Angolensis ◽  
Dpph Scavenging

The present study investigated the phytochemistry and antioxidant activity of Pterocarpus angolensis (Mubvamaropa) from Mupandawana. Pt. angolensis has numerous medicinal uses. The bark, sap, leaves and roots are all used to treat different ailments. The sap is used to stop nose bleeds, treatment of ulcers and kill ringworms. Several studies have supported the use of the tree’s sap to treat cataracts, malaria as well as skin inflammations. Pterocarpus angolensis bark was collected from Gutu, Masvingo province and authenticated by a plant taxonomist at the Zimbabwe National herbarium (Mr Chapano). The bark was cleaned using distilled water and oven dried at 40°C for 4 hours to constant weight. Size reduction was achieved using a Thomas-Wiley laboratory mill model with a 2 mm sieve mesh. The phytochemical analysis revealed that the ethanolic extract had more phyto-constituents than the water extract which only four phyto-constituents present all of which were weakly positive. DPPH scavenging activity was 95.11% at a concentration of 500 μg/mL bark extract, while that of the control, ascorbic acid, was 97.60%. Ascorbic acid had an IC50 value of 4.35 µg/mL while the bark extract had a value of 150.64 µg/mL.

scholarly journals Optimization of Ultrasonic Cellulase-Assisted Extraction and Antioxidant Activity of Natural Polyphenols from Passion Fruit

Molecules ◽  
2021 ◽  
Vol 26 (9) ◽  
pp. 2494
Author(s):  
Wei Wang ◽  
Yu-Ting Gao ◽  
Ji-Wen Wei ◽  
Yin-Feng Chen ◽  
Qing-Lei Liu ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Extraction Method ◽  
Ph Value ◽  
Soxhlet Extraction ◽  
Passion Fruit ◽  
Total Phenol Content ◽  
Extraction Temperature ◽  
Scavenging Activity ◽  
Solid Ratio ◽  
Dpph Scavenging

In this paper, ultrasonic cellulase extraction (UCE) was applied to extract polyphenols from passion fruit. The extraction conditions for total phenol content (TPC) and antioxidant activity were optimized using response surface methodology (RSM) coupled with a Box-Behnken design (BBD). The results showed that the liquid-to-solid ratio (X2) was the most significant single factor and had a positive effect on all responses. The ANOVA analysis indicated quadratic models fitted well as TPC with R2 = 0.903, DPPH scavenging activity with R2 = 0.979, and ABTS scavenging activity with R2 = 0.981. The optimal extraction parameters of passion fruit were as follows: pH value of 5 at 30 °C for extraction temperature, 50:1 (w/v) liquid-to-solid ratio with extraction time for 47 min, the experimental values were found matched with those predicted. Infrared spectroscopy suggested that the extract contained the structure of polyphenols. Furthermore, three main polyphenols were identified and quantified by HPLC. The results showed the content of phenolic compounds and antioxidant activity of the optimized UCE were 1.5~2 times higher than that determined by the single extraction method and the Soxhlet extraction method, which indicates UCE is a competitive and effective extraction technique for natural passion fruit polyphenols.

scholarly journals UJI AKTIVITAS ANTIOKSIDAN DAN PENENTUAN TOTAL KANDUNGAN FENOLIK EKSTRAK ETANOL DAUN NANAMUHA (Bridelia monoica Merr)

PHARMACON ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 178
Author(s):  
Julia Elsa Lakoro ◽  
Max R. J. Runtuwene ◽  
Paulina V. Y. Yamlean
Keyword(s):  
Antioxidant Activity ◽  
Extraction Method ◽  
Total Phenol ◽  
Total Phenolic ◽  
Total Phenol Content ◽  
Phenol Compounds ◽  
Activity Test ◽  
Ic50 Value ◽  
Dpph Method ◽  
Ethanol Extracts

ABSTRACTNanamuha (Bridelia monoica Merr.) is a plant that was used as a cancer and tumor drug by the Sangihe Islands community. This plant contains phenol compounds, flavonoids, alkaloids and tannins. The purpose of this study was to determine the potential of nanamuha leaves that grow in the Sangihe Island to have antioxidant activity and determine the total phenolic ethanol extracts of nanamuha leaf. The extraction method used is maceration with ethanol solvent p.a. The extracts obtained was determined total phenolic and antioxidant activity test using DPPH method. Based on the results obtained, the ethanol extracts of nanamuha leaves has antioxidant activity with an IC50 value of 75,03 µg / mL and the result showed that the total phenol content the ethanol extracts of nanamuha leaves of  53,34 mg / L.  Keywords : Nanamuha leaf, total phenol, antioxidant activity, DPPH  ABSTRAKNanamuha (Bridelia monoica Merr.) merupakan tanaman yang dimanfaatkan sebagai obat kanker dan tumor oleh masyarakat Kepulauan Sangihe. Tanaman ini memiliki kandungan senyawa fenol, flavonoid, alkaloid dan tanin. Tujuan dari penelitian ini adalah unutuk mengetahui potensi dari daun nanamuha yang tumbuh di Kepulaun Sangihe ini memiliki aktivitas antioksidan dan menentukan total fenolik dari ekstrak etanol daun nanamuha. Metode ekstraksi yang digunakan adalah maserasi dengan pelarut etanol p.a. Ekstrak yang diperoleh ditentukan total fenolik dan uji aktivitas antioksidan dengan metode DPPH. Berdasarkan hasil yang didapatkan ekstrak etanol daun nanamuha memiliki aktivitas antioksidan dengan nilai IC50 75,03 µg/mL dan hasil penelitian menunjukan total kandungan fenolik ekstrak etanol daun nanamuha sebesar 53,34 mg/L. Kata kunci : Daun Nanamuha, Total fenol, Aktivitas antioksidan, DPPH

scholarly journals Antimicrobial and antioxidant activity of Epimedium pinnatum

2013 ◽  
Vol 59 (2) ◽  
pp. 24-34 ◽  
Author(s):  
Mohaddese Mahboubi ◽  
Nastaran Kazempour ◽  
Hossein Hosseini ◽  
Mona Mahboubi
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Aqueous Extract ◽  
Methanol Extract ◽  
Ethanolic Extract ◽  
Total Phenolic ◽  
Spectrophotometric Methods ◽  
Ic50 Value ◽  
Antioxidant And Antimicrobial Activity ◽  
Antimicrobial And Antioxidant Activity

Summary Epimedium pinnatum (Berberidaceae family) is used as an aphrodisiac in traditional Chinese medicine (TCM). The aim of this study was to evaluate the antimicrobial and antioxidant activity of E. pinnatum extracts (ethanol, methanol and aqueous extracts). Total phenolic (TPC) and flavonoid contents (TFC) of each extract were assessed by spectrophotometric methods. It was exhibited that methanol extract had better antimicrobial activity than those of ethanolic extract or aqueous extract. The TPC and TFC of E. pinnatum extracts was higher in methanol extract (149 and 36.6 mg/g) than that of ethanolic extract (137.2 and 19.5 mg/g) and aqueous extract (86.2 and 8.4 mg/g). The methanol extract had lower IC50 value (200 µg/ml) than ethanolic (250 µg/ml) and aqueous extract (400 µg/ml). There was a positive correlation between TPC, TFC in E. pinnatum extract and their antioxidant and antimicrobial activity

scholarly journals Phytochemical analysis, antioxidant activity, and nephroprotective effect of the Raphanus sativus aqueous extract

2021 ◽  
Vol 11 (1) ◽  
pp. 84
Author(s):  
Hassan Zrouri ◽  
Amine Elbouzidi ◽  
Mohamed Bouhrim ◽  
Noureddine Bencheikh ◽  
Loubna Kharchoufa ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Aqueous Extract ◽  
Raphanus Sativus ◽  
Kidney Diseases ◽  
Body Weight Gain ◽  
Urinary Calcium ◽  
Control Group ◽  
Phytochemical Analysis ◽  
Distilled Water ◽  
Nephroprotective Effect

<p class="Default">In Morocco, Raphanus sativus is a widespread traditional medicinal plant used to treat various kidney diseases such as nephropathy.</p><p class="Mabstract"><strong>Objective</strong>:<strong> </strong>The present study aims to evaluate the protective effect of the <em>R. sativus </em>aqueous extract against the gentamicin-induced acute nephrotoxicity in rats.</p><p class="Mabstract"><strong>Methods</strong>: Rats were randomly separated into four groups (n=6; ♂/ ♀=1). The control group was treated only with distilled water (10 mL/kg; <em>p.o</em>). The gentamicin group was treated with distilled water (10 mL/kg; <em>p.o</em>) and injected intraperitoneally by the gentamicin (80 mg/kg; <em>i.p</em>). <em>R. sativus </em>groups were treated with the aqueous extracts of this plant at a dose of (200 or 400 mg/kg; <em>p.o</em>) and injected by the gentamicin (80 mg/kg; <em>i.p</em>). The plasma Creatinine, Urea, Uric Acid, Albumin, Total Protein, Alanine transaminase, Aspartate transaminase, and Calcium levels were measured. The urinary creatinine, urinary Calcium, urinary volume, water intake, creatinine clearance, body weight gain, relative right kidney weight, and kidney malondialdehydes were determined.</p><p class="Mabstract"><strong>Results</strong>: This study showed that the daily pretreatment with <em>R. sativus</em> aqueous extract at two doses of 200 and 400 mg/kg, p.o prevented the rats from the gentamicin-induced nephrotoxicity. Moreover, the <em>R. sativus </em>aqueous extract showed a high amount of polyphenols and flavonoids and a significant antioxidant activity.</p><p class="Mabstract"><strong>Conclusion</strong>: <em>R. sativus </em>aqueous extract, as a novel natural product, may have preventive properties against gentamicin-induced nephrotoxicity in rats. The present study describes new areas of investigation to introduce better therapeutic agents for renal disorders and dysfunction.</p>

scholarly journals Phytochemical analysis and antioxidant activity of different plant parts of Pellacalyx axillaris

2019 ◽  
Vol 15 (3) ◽  
pp. 394-397
Author(s):  
Najwa Ahmad Kuthi ◽  
Norazah Basar
Keyword(s):  
Antioxidant Activity ◽  
Biological Activities ◽  
Radical Scavenging ◽  
Bark Extract ◽  
Phytochemical Analysis ◽  
Phytochemical Study ◽  
Antioxidant Power ◽  
Plant Parts ◽  
Crude Extracts ◽  
Radical Scavenging Assay

Pellacalyx axillaris or locally known as ‘membuloh’ is a mangrove species belonging to the Rhizophoraceae family. Till date, there has been only one phytochemical study found on this particular plant species and that without any documentation on its biological activities. Therefore, the present work aimed to reveal the phytoconstituents and the antioxidant activity of different crude extracts from different plant parts of P. axillaris. Experimentally, three organic solvents of different polarities i.e. n-hexane, ethyl acetate and methanol were used to prepare the crude extracts from the dried leaves, twigs and barks of P. axillaris. The preliminary phytochemical screening of this species indicated the presence of terpenoids, phenolic compounds, tannins, flavonoids, alkaloids, anthraquinone glycosides and carbohydrates. The in vitro antioxidant activity of the species evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay, 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radical scavenging assay, and ferric reducing antioxidant power (FRAP) suggested that the methanolic bark extract contained potential source of natural antioxidants. Further research into isolation of antioxidant compounds from this species is highly recommended.

scholarly journals PHYTOCHEMICAL ANALYSIS, ANTIMICROBIAL AND ANTIOXIDANT ACTIVITIES OF THYMUS VULGARIS L.

2020 ◽  
Vol 1 (2) ◽  
Author(s):  
Moaiza Iftikhar ◽  
Salma Batool
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Biologically Active ◽  
Thymus Vulgaris ◽  
Plant Sample ◽  
Phytochemical Analysis ◽  
Bacterial Strains ◽  
Anti Oxidant ◽  
Dpph Scavenging ◽  
Dpph Scavenging Assay

Medicinal activities of Thymus vulgaris L. were studied in the underlying research which was conducted in University of Central Punjab, Lahore. The powdered plant sample was collected and subjected to extraction and filtration by dissolving it in seven different solvents and thus the fractions obtained, were used for further analysis. The qualitative and quantitative analysis was carried out which revealed that this plant contained many biologically active compounds like saponin, alkaloids etc. These compounds have enormous bioactive roles. Antimicrobial activity was also determined in which four bacterial strains were used and the susceptibility of all the fractions of plant extract was determined by their inhibition zones which were calculated in mm. The results showed that water and chloroform extracts of plant has highest activity. The antioxidant activity was also determined by DPPH scavenging assay for which different concentrations of all plant extracts were made and their absorbance was noted to measure the percentage inhibition of each concentration, which revealed that this plant has significant antioxidant activity. The results have been represented using statistical approach that revealed that T. vulgaris plant has significant antibacterial and anti-oxidant activities and phytochemical analysis also proved that it has many useful compounds which have voluminous biological activity and these could be used in the formation of novel drugs.

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