scholarly journals Antioxidants from Callus Technology

Author(s):  
Gali Adamu Ishaku ◽  
Ftepti Benson Jelani ◽  
Kyugah Jacob Tersur ◽  
Audu Sanusi Kiri

Antioxidants are very important compounds that are very vital in human health and they have been proven to reduce the risk of diseases such as cancer in human health. Many researchers have used callus to produce antioxidant and most of them used different techniques to get reasonable amounts of antioxidants. The technique used determines the number of antioxidants that will be produced from any explants. Callus Technology involves the techniques of producing callus and metabolites in the presence of explants using different plant hormonal combination in media, different environmental culture condition (light, relative humidity and tempreture), use of elicitors and under a sterile conditions. Callus technology is very promising due to its ability to produce a larger quantity of metabolites (antioxidants) compare to the raw extract of its explants. The use of callus to produce antioxidants is very important and very useful in discovering new plants as a source of antioxidants. The use of callus technology was reviewed for production of antioxidant from the callus of the following plants: Sericostoma pauciflorum, Helicteres angustifolia L, Lepidium sativum L, Randia echinocarpa, Andrographis paniculata Nees, Citrullus colocynthis, Rauwolfia vomitoria Afzel, Decalepis hamiltonii, Bacopa monnieri (Linn.) and Isodon rugosus (Wall. Ex Benth). Callus technology can be utilized to produce antioxidants and other metabolites in industrial quantity. Most of the metabolites from plants have been found to have medicinal values or useful to mankind and antioxidant is one of them.

Ecotoxicology ◽  
2021 ◽  
Vol 30 (2) ◽  
pp. 277-291
Author(s):  
D. Montvydienė ◽  
A. Jagminas ◽  
Ž. Jurgelėnė ◽  
M. Kazlauskas ◽  
R. Butrimienė ◽  
...  

Environments ◽  
2021 ◽  
Vol 8 (6) ◽  
pp. 49
Author(s):  
Markéta Šourková ◽  
Dana Adamcová ◽  
Jan Winkler ◽  
Magdalena Daria Vaverková

Illegal dumps and landfills with disposed of tires are a fact of today, which should not be neglected as they represent a great ecological burden for the environment, affect the surrounding nature and disturb the landscape. This research was focused on testing the phytotoxicity of aqueous leachates from the fractions of tires in two sets of experiments—to simulate laboratory conditions (tire leaching in distilled water) and natural conditions (tire leaching in water from a recipient) using the Phytotoxkit testing kit (kit for the establishment of inhibition/stimulation effect on the root development) and the watercress test of phytotoxicity (biological method for the assessment of leachate phytotoxicity). Plants whose seeds were selected for the test were watercress (Lepidium sativum L.) and white mustard (Sinapis alba L.). The aqueous leachate was tested for 38 weeks. During the experiment, physical and chemical parameters were measured at intervals of 14 days by the testing instrument HACH TEST KIT: electric conductivity (EC), amount of dissolved oxygen (LDO) and pH. Results of root growth inhibition (IR) on the seeds of Lepidium sativum L. and Sinapis alba L. exhibited values ranging from 11.73% to 47.74% in the tested samples. Results of germination index (GI) on the seeds of Lepidium sativum L. exhibited values below 66% in the tested samples, which indicated the leachate phytotoxicity. In spite of the fact that similar studies are tackling the acute toxicity of leachates from tires (particularly to algae, embryos and animals), this research brings complementary information in testing the acute phytotoxicity of tire leachates to higher plants.


1982 ◽  
Vol 37 (9) ◽  
pp. 793-801 ◽  
Author(s):  
Rudolf Karl ◽  
Wolfhart Rüdiger

Extracts from oat husks inhibit germination of a variety of seeds including Avena sativa L., Sorghum spec., Phalleris spec., Raphanus spec., Amaranthus caudatus, Lepidium sativum L. A quantitative assay for this inhibition was developed on the basis of percentage of root growth of Avena in the presence of extracted material compared with root growth of water controls. Fractionation of the extracts revealed that about half of the total inhibitory activity was found in the fraction of free organic acids. The inhibition was not due to known inhibitors. Abscisic acid. was not found in this extract. Phenole carbonic acids were determined in the extract. Their con­centration was too low to significantly contribute to the observed inhibitory activity.


1999 ◽  
Vol 27 (03n04) ◽  
pp. 397-405 ◽  
Author(s):  
S. E. I. Adam

A toxicity study was made on Lepidium sativum L. seeds used in Saudi traditional medicine for the treatment of various ailments. Lepidium sativum L. seed fed to Wistar albino rats at 2% (w/w) was non-toxic, Ten percent (w/w) was toxic but not fatal and 50% (w/w) of the diet for 6 weeks was lethal and caused depression in growth rate and entero-hepato-nephrotoxicity. Organ lesions accompanied by anemia and leukopenia were correlated with alterations in serum AST and ALT activities and concentrations of total protein, cholesterol, urea, and other serum constituents.


Author(s):  
DA Narutdinov ◽  
RS Rakhmanov ◽  
ES Bogomolova ◽  
SA Razgulin

Introduction: Extreme climate conditions have a negative impact on human health. Purpose: The study aimed to assess weather and climate-related risks to human health in different areas of the Krasnoyarsk Region by effective temperatures estimated during two long-term observation periods. Materials and methods: We analyzed ambient temperatures (average monthly and minimum), wind speed (average and maximum), and relative humidity in the subarctic and temperate continental zones estimated during the periods of determining climatic norms in 1961–1990 and 1991–2020. The health risk was assessed on the basis of effective temperatures. Results: In the subarctic zone, the wind strength (average and maximum values) decreased, the duration of such periods increased just like the ambient temperature while the relative humidity did not change. In temperate climates, all indicators have changed. In the subarctic zone, in the second observation period, frostbite was possible within 20–30 minutes during two months (versus 3 in the first). In the temperate climate, there was no such risk to humans. At the minimum temperature and maximum wind speed in the subarctic zone, the risk of frostbite is possible during 5 months (versus 6): after 10–15 minutes during two months and after 20–30 minutes – during three months of the year. In temperate climates, frostbite is possible within 20–30 minutes during two months (versus 3 in the first period). Conclusions: In the interval of establishing climatic norms (1991–2020), a significant increase in effective temperatures was determined: in the subarctic zone with the average wind strength and temperature in February–April and June, with maximum wind and minimum temperature – in March–July; in temperate climates, in April and June, respectively. The duration of periods of health risks posed by cold temperature exposures in the subarctic climate with average wind and temperature values equaled two months (I–II), with maximum wind speed and minimum temperatures – five months (XI–III); in the temperate climate, it was null and 2 (3) months (I, II, and XII), respectively.


2018 ◽  
Vol 16 ◽  
pp. 32-40 ◽  
Author(s):  
Legesse Tadesse ◽  
Firew Mekbib ◽  
Adugna Wakjira ◽  
Zerihun Tadele

Molecules ◽  
2021 ◽  
Vol 26 (22) ◽  
pp. 6842
Author(s):  
Kamila Rybczyńska-Tkaczyk

The aim of this study was to evaluate the bioremoval of anthracycline antibiotics (daunomycin-DNR, doxorubicin–DOX, and mitoxantrone-MTX) by immobilized mycelium of B. adusta CCBAS 930. The activity of oxidoreductases: versatile peroxidases (VP), superoxide dismutase (SOD), catalase (CAT), and glucose oxidase (GOX), and the levels of phenolic compounds (PhC) and free radicals (SOR) were determined during the biotransformation of anthracyclines by B. adusta strain CCBAS 930. Moreover, the phytotoxicity (Lepidium sativum L.), biotoxicity (MARA assay), and genotoxicity of anthracyclines were evaluated after biological treatment. After 120 h, more than 90% of anthracyclines were removed by the immobilized mycelium of B. adusta CCBAS 930. The effective biotransformation of anthracyclines was correlated with detoxification and reduced genotoxicity.


2017 ◽  
Vol 34 (1) ◽  
pp. 95-102 ◽  
Author(s):  
Tomasz Ciesielczuk ◽  
Czesława Rosik-Dulewska ◽  
Joanna Poluszyńska ◽  
Irena Sławińska

Abstract The study presents the results of research on the acute toxicity of a fertilizer formulas made of spent coffee ground (SCG) with addition of ash from low-temperature combustion of biomass or ash with an admixture of magnesium sulphate and blood meal. The experimental fertilizer formulas included also rape oil used as a plasticizer for controlling the nutrients release from the fertiliser. Mustard (Sinapis alba L.), oats (Avena sativa sp. L.), cucumber (Cucumis sativus L.) and cress (Lepidium sativum L.) were used as test plants species in the experiment. The toxicity tests were performed using a standard procedure of 72 h with the use of Phytotoxkit microbiotest and fertilizer application of 2.5; 5 and 10% (v/v). The obtained results indicated an increase of acute toxicity for all tested plant species, proportionally to the applied doses of the fertilizer. During the 72 h period, the strongest inhibition of seedling growth was recorded in samples consisting of 10% of the tested fertilizers, particularly when they showed considerable level of salinity or low pH values. From the tested plant species, cress (Lepidium sativum L.) turned out to be the most sensitive to the applied fertilizers, the least was cucumber (Cucumis sativus L.) for which only a small inhibition of root system growth was observed. The inhibited growth of roots could be attributed to a reduced oxygen access and excessive salinity of the substratum caused by the applied additives.


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