scholarly journals Isolation and Identification of Microsporum canis in Companion Animals from Selected Local Government Areas in Abia State

2020 ◽  
pp. 9-16
Author(s):  
Nwiyi, Paul Okechukwu ◽  
Ottah, Betsy
Keyword(s):  
Local Government ◽  
Companion Animals ◽  
Thick Wall ◽  
Microsporum Canis ◽  
Frequency Of Occurrence ◽  
Isolation Rate ◽  
Isolation And Identification ◽  
Light Yellow ◽  
Cotton Blue ◽  
Demographic Indices

The aim of the study is to isolate and identify Microsporum canis from companion animals (dogs and cats) in three local government areas of Abia State. A total of one hundred and fifty skin scrapings from infected dogs (100) and cats (50) were screened. Saboruad destroxe agar was used for the culture and Needle mount technique was adopted. Lactophenol Cotton Blue (LCB) was used for staining. Demographic indices like; age, sex, and breed of the animals were considered. This organism at macroscopy appears as white, light yellow, cottony to powdery colonies. At microscopic view, the spores of M. canis appear as large and spindle shaped with thick wall. The dogs has a predominant isolation rate of 36.0%.The female dogs and cats presented the highest frequency of occurrence at 58.2% and 63.6% respectively. Dogs of 9months old and above had more M. canis isolation rate at 70.0%, while cats between 5 and 8months of age had the highest isolation rate at 33.3%. Dogs and cats at 1 to 4 months of age had the least M. canis isolation rate at 7.5% and 14.5% respectively. The indigenous breeds of dogs had the highest isolation rate of M. canis at 53.8% while the Caucasian breed was the least at 7.7%. Statistical analysis shows that (p=.05) there is significance in isolation rate of M. canis in dogs and cats.

scholarly journals Isolation and identification of common fungal spp. from commercial broiler feeds available in market of Sylhet District, Bangladesh

2016 ◽  
Vol 4 (2) ◽  
pp. 38-41 ◽  
Author(s):  
MT Islam ◽  
MK Hossain ◽  
ATMM Elahi ◽  
M Purkayastha ◽  
MM Rahman
Keyword(s):  
Fungal Species ◽  
Natural Sciences ◽  
Local Market ◽  
Frequency Of Occurrence ◽  
Isolation And Identification ◽  
Fungal Isolates ◽  
Commercial Broiler ◽  
Rhizopus Sp ◽  
Feed Samples ◽  
Broiler Feed

The present study was designed to investigate the mycological contamination of commercial broiler feeds used in poultry establishments in sylhet, Bangladesh. The feed samples of commercial broiler feed (Starter, Grower and Finisher) were collected from the different areas of Sylhet district. A total of 189 commercial broiler feed samples where 63 Starter, 63 Grower and 63 Finisher were collected from the different areas of local market in Sylhet. The selected areas were Kadamtali, Shibjong, Khadim, Kamal Bazar, Dakshin Surma, Fenchugonj. From the feed samples analyzed for the presence of fungal agents, 144 (76.2%) were found positive for one or more fungal species. Fungal isolates were found among 36 (57%) of the 63 Starter feed samples, 45 (71.4%) of the 63 Grower feed samples and 63 (100%) of the 63 Finisher feed samples. The fungal agents isolated from Broiler Starter Feeds, Aspergillus spp. 51 (70.8%) has the highest frequency of occurrence, followed by Fuserium spp. 12 (16.7%) and least is Rhizopus sp. 9(12.5%). Similarly, in case of Broiler Grower Feeds, Aspergillus spp. 66 (68.8%) has the highest frequency of occurrence, followed by Fuserium spp. 18 (18.7%) and least is Rhizopus sp. 12(12.5%). In case of Broiler Finisher Feeds, Aspergillus spp. 90 (69.8%) has the highest rate of occurrence followed by Fuserium spp. 24 (18.6%) and least is Rhizopus sp. 15 (11.6%) respectively.International Journal of Natural Sciences (2014), 4(2) 38-41

scholarly journals Isolation and identification of fungal assemblages in the necrotic spots of Bambusa pallida (L.) Voss

2018 ◽  
Vol 7 (4) ◽  
pp. 2160
Author(s):  
Sanjit Debnath ◽  
Pintu Karmakar ◽  
Sukla Bhattacharjee ◽  
Koushik Majumdar ◽  
Panna Das ◽  
...  
Keyword(s):  
Rural Economy ◽  
Present Finding ◽  
Leaf Spot Disease ◽  
Curvularia Lunata ◽  
Isolation Rate ◽  
Isolation And Identification ◽  
Good Source ◽  
Fusarium Redolens ◽  
Necrotic Spots ◽  
Fungal Assemblages

Bamboos are the most important plant due to their different uses and good source of income for upliftment of rural economy. Different parts of bamboos are damaged by many harmful bacteria, fungi and other organisms causing huge loss of economy. Diseased leaves of Bambusa pallida were used for isolation of fungal assemblages in symptom sites. A total of 7 fungi were isolated from the cultured medium; these were Lasiodiplodia theobromae, Drechslera rostrata, Curvularia lunata, Fusarium redolens, Sterile sp1, Sterile sp2, and Sterile sp3. Isolated L. theobromae, D. rostrata, C. lunata, F. redolens may be the causal fungi for necrotic spots disease in B. pallida. Colonization rate and isolation rate (%) were observed highest in L. theobromae, C. lunata F. redolens and Sterile sp2. The present finding will be helpful for knowing the causes of damage and relation with leaf spot disease. Further studies needed to be undertaken for finding the mode of colonization to causes diseases.

scholarly journals Establishment and Characterization of a High and Stable Porcine CD163-Expressing MARC-145 Cell Line

2018 ◽  
Vol 2018 ◽  
pp. 1-9
Author(s):  
Xiangju Wu ◽  
Jing Qi ◽  
Xiaoyan Cong ◽  
Lei Chen ◽  
Yue Hu ◽  
...  
Keyword(s):  
Disease Management ◽  
Cell Line ◽  
Immunofluorescence Assay ◽  
Productive Infection ◽  
Cellular Receptor ◽  
Cell Tropism ◽  
Vaccine Production ◽  
Isolation Rate ◽  
Isolation And Identification

Isolation and identification of diverse porcine reproductive and respiratory syndrome viruses (PRRSVs) play a fundamental role in PRRSV research and disease management. However, PRRSV has a restricted cell tropism for infection. MARC-145 cells are routinely used for North American genotype PRRSV isolation and vaccine production. But MARC-145 cells have some limitations such as low virus yield. CD163 is a cellular receptor that mediates productive infection of PRRSV in various nonpermissive cell lines. In this study, we established a high and stable porcine CD163- (pCD163-) expressing MARC-145 cell line toward increasing its susceptibility to PRRSV infection. Indirect immunofluorescence assay (IFA) and Western blotting assays showed that pCD163 was expressed higher in pCD163-MARC cell line than MARC-145 cells. Furthermore, the ability of pCD163-MARC cell line to propagate PRRSV was significantly increased as compared with MARC-145 cells. Finally, we found that pCD163-MARC cell line had a higher isolation rate of clinical PRRSV samples and propagated live attenuated PRRS vaccine strains more efficiently than MARC-145 cells. This pCD163-MARC cell line will be a valuable tool for propagation and research of PRRSV.

scholarly journals Isolation and Identification of Soil Mycoflora in the Upland and Lowland Soils of Usmanu Danfodiyo University, Sokoto, Sokoto State

2018 ◽  
pp. 1-7
Author(s):  
I. Y. Tafinta ◽  
K. Sheh ◽  
H. M. Maishanu ◽  
S. S. Noma ◽  
S. A. Yusif ◽  
...  
Keyword(s):  
Aspergillus Niger ◽  
Fusarium Oxysporum ◽  
Trichoderma Harzianum ◽  
Fungal Species ◽  
Potato Dextrose Agar ◽  
Morphological Features ◽  
Rhizopus Stolonifer ◽  
Isolation And Identification ◽  
Dilution Plate ◽  
Cotton Blue

A total of 14 different fungal species belonging to 7 genera were isolated from the upland and lowland soils of Usmanu Danfodiyo University, Sokoto, between October to December, 2015. The mycoflora were isolated using dilution plate technique on Potato dextrose agar amended by 1% streptomycin. Identification was made microscopically using the lacto-phenol cotton blue method and macroscopically by comparing the cultural and morphological features with the help of authentic fungal manual and taxonomic key. The identified species are; Aspergillus niger, A. flavus, A. fumigatus, A. nidulans, A. oryzae, Alternaria longifes, Fusarium oxysporum, F. solani, F. mangifera, Rhizopus stolonifer, R. orizae, Saccharomyces cerevisae, Trichoderma harzianum and Trichophytum quallinum. The highest number of fungi (50 isolates) were obtained from lowland uncultivated soils (D), followed by the upland uncultivated soils (B) with about 34 strains, and the least (22 isolates) were obtained from upland cultivated soils (A) out of the total 132 isolates. Variations between sites were statistically analyzed.

scholarly journals A Survey of Trypanosome Species Circulating In Pigs In Three Selected Local Government District of Enugu State, Nigeria

2018 ◽  
Vol 1 (2) ◽  
pp. 136-143
Author(s):  
J.N. Omeje ◽  
J.S. Akinbobola
Keyword(s):  
Local Government ◽  
Local Governments ◽  
Age Groups ◽  
Buffy Coat ◽  
Isolation Rate ◽  
Cross Sectional Survey ◽  
Cross Sectional ◽  
Predominant Species ◽  
Thin Smear ◽  
Government District

A cross sectional survey of trypanosomosis of pigs was carried out in three randomly selected Local Government Areas (Nsukka, Udenu and Igbo Eze South) out of the six local government areas in Enugu North Senatorial Zone. Blood samples were randomly collected from a total of 1800 pigs from abattoirs and farms and were examined for trypanosomes parasites using standard parasitological methods: wet film, buffy coat and Giemsa-stained thin smear preparations. A total of 93 (5.2%) pigs were positive overall. Among the positive samples, Trypanosoma brucei was the predominant species (86.0%), followed by T. congolense (13.9%). The different relative isolation rate for Nsukka, Igbo-Eze South and Udenu Local government areas were 14 (4.7%), 14 (4.7%), and 19 (4.0%) respectively in the dry season, and 17 (5.7%), 17 (5.7%) and 12 (6.3%) in the rainy season respectively. The trypanosome prevalence in males from Nsukka, Igbo-Eze South and Udenu local governments were 6.5%, 6.2% and 4.2% respectively, while report in female were 3.8%, 4.1% and 5.9% in the three local governments respectively. There was no significant variations (p<0.05) with seasons, sex and age groups. It was therefore concluded that porcine trypanosomosis is still endemic in Enugu North Senatorial Zone with T. brucei being the most incriminated as the cause of the disease in the zone.

scholarly journals Isolation and identification of some pathogenic species of Fungi from Shatt-al-Arab River

2021 ◽  
Vol 2063 (1) ◽  
pp. 012021
Author(s):  
Shrouk Abdulrazak Hassan Al-Ibraheem ◽  
Angham O S Al-Zeadei
Keyword(s):  
Aspergillus Niger ◽  
Aspergillus Flavus ◽  
Room Temperature ◽  
Pathogenic Fungi ◽  
Pathogenic Species ◽  
Isolation And Identification ◽  
Cotton Blue ◽  
Blue Stain ◽  
The Media ◽  
Aspergillus Candidus

Abstract This study aimed to isolation and identification of pathogenic fungi from Shatt – al-Arab River in Basra city, Fourteen water samples were collected from different area from Shatt-al-Arab River (AL Ashar, AL Tnoma, AL Makal, AL Qurna, AL Karma, AL Jabiluh, AL-Hartha), from October to December in 2017, with 250 ml volume, this samples centrifuged at 5000 rpm for 10 min at room temperature, the floating was removed and then take the precipitate and pour directly into the center of the media of SDA and PDA and then incubation in a temperature range25-27c for 4 days after that the growth on the media made pure culture and each fungi species diagnosed based on the cultural and microbiological phenotypes, smear prepared with lacto phenol cotton blue stain and the results show 57.1% of growth was Aspergillus niger, 85.7% Aspergillus flavus and 42.8% was Aspergillus candidus and 14.2% was Rhizopus, while the results show 42.8% of growth was Penicillium..

Isolation and Identification of Hyperoside in Abelmoschus manihot Flower

2013 ◽  
Vol 781-784 ◽  
pp. 880-883
Author(s):  
Yan Qiu ◽  
Ming Zhu Wang
Keyword(s):  
Thin Layer ◽  
Formic Acid ◽  
Ethyl Acetate ◽  
Gradient Elution ◽  
Isolation And Identification ◽  
Ethanol Extraction ◽  
Light Yellow ◽  
Yellow Stripe ◽  
Layer Chromatography ◽  
Chloroform Methanol

Abelmoschus Manihot (L.) Medic flowers are traditionally used for food and medicinal materials. With the aim of acquiring the best method of isolating hyperoside from total flavone in A. Manihot flowers, this study investigated the effects of different eluted factors on separating hyperoside in total flavone of crude extracts. Gradient elution was used to separate the total flavone of ethanol extraction. The eluates were isolated by silica gel thin-layer chromatography (TLC) and identified by HPLC. The results showed that the elution components (chloroform: methanol = 50: 50) isolated by TLC (developing agent ethyl acetate: formic acid: water = 8:3:1) presented a clear light yellow stripe which was identified as hyperoside by HPLC.

Isolation and Identification of Microorganisms Associated with Bioremediation of Oil Spilled Site in Bodo West, Rivers State, Nigeria.

2019 ◽  
pp. 1-8
Author(s):  
Tombari Bodo ◽  
Lekpa Kingdom David ◽  
Batombari Gbidum Gimah
Keyword(s):  
Aspergillus Niger ◽  
Local Government ◽  
Bacillus Anthracis ◽  
Micrococcus Luteus ◽  
Mineral Salt ◽  
Morphological Observation ◽  
Isolation And Identification ◽  
Streptococcus Faecalis ◽  
Rivers State

The samples collected from an oil spilled sites in Bodo West in Gokana Local Government of Rivers State in Nigeria were isolated to identify microorganisms associated with bioremediation. The population of about 311 different forming colonies were recorded in the study area; out of which 18 distinctive colonies were identified based on their morphological observation. From the selected isolates, 10 of them were assumed to be degraders because they form maximum clear zones on the mineral salt media. The results of the analysis show that notable number of microorganism of which seven bacteria and seven fungi were isolated and identified. The bacteria are Micrococcus luteus, Streptococcus lactic, Streptococcus epidemidis, Streptococcus faecalis, Clostridium sprogenes, Aerococcus viridems, and Bacillus anthracis. The fungi are Articulosspara inflate, Dendospora erecta, Aspergillus niger, Liodioderium Species, Geotichrum albdum, Aspergillus funigatus and Sreptothric atrax. On the strength of the result, it is inferred that microorganisms are associated with bioremediation and can be used for environmental and petroleum cleanup exercise in an oil spilled site.

scholarly journals Patterns of Antimicrobial Resistance Observed in Escherichia coli Isolates Obtained from Domestic- and Wild-Animal Fecal Samples, Human Septage, and Surface Water

2005 ◽  
Vol 71 (3) ◽  
pp. 1394-1404 ◽  
Author(s):  
Raida S. Sayah ◽  
John B. Kaneene ◽  
Yvette Johnson ◽  
RoseAnn Miller
Keyword(s):  
Escherichia Coli ◽  
Surface Water ◽  
Antimicrobial Resistance ◽  
Antimicrobial Agent ◽  
Companion Animals ◽  
Diffusion Method ◽  
Resistant Bacteria ◽  
Isolation And Identification ◽  
Fecal Samples ◽  
E Coli

ABSTRACT A repeated cross-sectional study was conducted to determine the patterns of antimicrobial resistance in 1,286 Escherichia coli strains isolated from human septage, wildlife, domestic animals, farm environments, and surface water in the Red Cedar watershed in Michigan. Isolation and identification of E. coli were done by using enrichment media, selective media, and biochemical tests. Antimicrobial susceptibility testing by the disk diffusion method was conducted for neomycin, gentamicin, streptomycin, chloramphenicol, ofloxacin, trimethoprim-sulfamethoxazole, tetracycline, ampicillin, nalidixic acid, nitrofurantoin, cephalothin, and sulfisoxazole. Resistance to at least one antimicrobial agent was demonstrated in isolates from livestock, companion animals, human septage, wildlife, and surface water. In general, E. coli isolates from domestic species showed resistance to the largest number of antimicrobial agents compared to isolates from human septage, wildlife, and surface water. The agents to which resistance was demonstrated most frequently were tetracycline, cephalothin, sulfisoxazole, and streptomycin. There were similarities in the patterns of resistance in fecal samples and farm environment samples by animal, and the levels of cephalothin-resistant isolates were higher in farm environment samples than in fecal samples. Multidrug resistance was seen in a variety of sources, and the highest levels of multidrug-resistant E. coli were observed for swine fecal samples. The fact that water sample isolates were resistant only to cephalothin may suggest that the resistance patterns for farm environment samples may be more representative of the risk of contamination of surface waters with antimicrobial agent-resistant bacteria.

Continuous Source Outbreak of Campylobacteriosis Traced to Chicken

2000 ◽  
Vol 63 (3) ◽  
pp. 309-314 ◽  
Author(s):  
ANDREW D. PEARSON ◽  
MELODY H. GREENWOOD ◽  
JACKIE DONALDSON ◽  
TIMOTHY D. HEALING ◽  
DENNIS M. JONES ◽  
...  
Keyword(s):  
Local Population ◽  
Hygiene Practice ◽  
Frequency Of Occurrence ◽  
Epidemiological Investigation ◽  
Serotype Distribution ◽  
Isolation Rate ◽  
Specific Strain ◽  
Human Campylobacteriosis ◽  
Index Cases ◽  
Continuous Source

Poultry is a source of human campylobacteriosis, but a large continuous source outbreak, heretofore, has not been attributed to both a single source of poultry and single serotype of Campylobacter. Here we report an outbreak of C. jejuni affecting 6 catering college trainees and 13 patrons of a restaurant in southern England. An epidemiological investigation successfully tracked the outbreak source to the farm of origin. Frequency of occurrence of campylobacters and outbreak serotype distribution were determined in index cases, the local population, and local chicken suppliers. The source farm was investigated and the effect of interventions assessed. A single outbreak serotype of C. jejuni was isolated from trainee chefs, patrons, and chicken supplied to the college by Wholesaler A. The Campylobacter isolation rate for Wholesaler A was 89% (98% outbreak serotype), compared to 40% for non-Wholesaler A (10% outbreak serotype). The isolation rate for 14 months averaged 85% (99% outbreak serotype) in chickens grown on two farms (X and Y) supplying Wholesaler A, contributing ∼40% to all local cases. In the research reported here, a specific strain and hygiene practice were found to be important for understanding transmission of Campylobacter from poultry to humans in this outbreak.

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