scholarly journals Increase of Nonspecific Immunity and Productivity of Piglets when using the Probiotic Lactobacillus reuteri

Author(s):  
Ovcharova Anastasiya ◽  
Ostrenko Konstantin ◽  
Kutin Ivan

The stress of weaning piglets negatively affects all factors of the body. First of all, this affects the violation of nutrition and a decrease in the activity of the immune system. The use of probiotics allows to reduce the negative consequences caused by weaning from a sow. The aim of the study was to study the effect of probiotics based on Lactobacillus reuteri on the indicators of nonspecific resistance and productivity of piglets. The obtained data showed a high efficiency of the use of probiotics. In the experimental group, the phagocytic activity of neutrophils increased by 4.4%, the phagocytic index was 6.6% higher, and the bactericidal activity increased by 29% compared to the control group. In piglets treated with Lactobacillus reuteri, body weight increased by 9.3%, and the average daily gains in the control group increased by 15.8%. The use of probiotics in the period of stress after weaning allows you to activate the immune status.

Author(s):  
I.I. Okulova ◽  
◽  
I.A. Domsky ◽  
Yu.A. Berezina ◽  
Z.N. Beltyukova ◽  
...  

The use of «Arkusit» at a dose of 20 μg / kg of body weight indicates a beneficial effect on the factors of nonspecific resistance of the silver-black fox. The concentration of alkaline phosphatase in the blood serum in the experimental group decreased by 1.8 times (P <0.001), the ALT index - by 1.2 (P <0.05). The concentration of glucose in the experimental group increased 2 times (P <0.05) compared with the control group. In animals of the experimental group, the lysozyme activity of blood serum increased 1.3 times (P <0.05), the OPR reaction and the phagocytic index - 1.4 times (P <0.05), the bactericidal activity of blood serum - 1.8 times (P <0.05). «Arcusit» stimulates the synthesis of gamma globulins, increases the opsonophagocytic, bactericidal activity of blood serum. Thus, the analysis of experimental data indicates that the drug «Arcusit» has a pronounced immunostimulating effect, providing the activation of factors of nonspecific resistance of the body of the silver-black fox.


2021 ◽  
Vol 4 (2) ◽  
pp. 31-37
Author(s):  
O. P. Dobrianska ◽  
M. I. Zhyla ◽  
O. I. Vishchur ◽  
O. V. Deren ◽  
M. Z. Koryliak

Currently, it is important in aquaculture to study and use products and additives, which have positive effects on the digestibility of nutrients by helping to normalize the intestinal microflora, thereby modulating fish immune response. The aim of the study was to assess the effect of a prebiotic based on mannan oligosaccharides produced from the outer walls of Saccharomyces cerevisiae cells on the histological structure, morphometric state of the intestine and activity of cellular and humoral components of nonspecific resistance of age -2+ carp. The experiment was conducted in four analogous ponds, which were stocked with age-1 scaly carp with an average weight of 55–58 g with a stocking density of 1000 fish/ha. Carp had been fed balanced compound feed for 60 days. Experimental groups of fish were fed with a prebiotic as a feed additive in the amount of: 0.025 % – Experiment 1, 0.05 % – Experiment 2 and 0.075 % – Experiment 3. Use of the studied prebiotic did not damage the intestinal histological structure of carp of the experimental groups. The villi height and intestinal crypt depth of carp in Experiment 1 (P < 0.05) and Experiment 2 (P < 0.01) were larger compared to the control group. Experiment 3 showed destructive changes in intestinal mucosa. The results of histological examination indicated the effectiveness of the use of the studied prebiotic in the amount of 0.025 and 0.05 % in the diet of age -1+ carp due to the absence of pathological changes and active morphofunctional state of the intestine, which, in turn, had a positive effect on feed absorption and metabolic processes in fish body. Analysis of the indicators of cellular and humoral links of natural resistance showed that in carp of the second and third experimental groups lysozyme activity of serum was 5 and 11 % (P < 0.001) higher than in the control group. Similar changes, though found in a greater extent, were recorded in the study of bactericidal activity of blood serum, especially in individuals of the second and third experimental groups (by 6.8 (P < 0.01) and 15.2 %). While with carps of the third experimental group, which used a prebiotic drug in the amount of 0.075 %, the content of CEC was 20.5 % higher than in the control group, which indicates an additional antigenic load on the body and is an unfavorable diagnostic factor. At the same time, with the fish of the second experimental group, which received, respectively, 0.05 % of the supplement of the drug “Actigen” to the main diet, probably higher phagocytic activity of blood neutrophils was recorded. A direct dependence of the growth of the phagocytic index and the phagocytic number of neutrophils on the dose of the studied prebiotic was detected. Immunological studies indicated a dose-dependent effect of the prebiotic on the activity of cellular and humoral components of non-specific resistance of carp. Given this, for the effective functioning of innate protective mechanisms, for the increase of the body's immune potential and productivity, it is most appropriate to apply to the diet of carp prebiotic supplement “Actigen” in the amount of 0.025 and 0.05 %.


Author(s):  
R. Milushev ◽  
D. Engovatov ◽  
V. Engovatov

A protein-vitamin-mineral concentrate (PVMC) containing plant protein has been developed for balancing compound feed for early weaned piglets. It uses a component from complete-fat fl axseeds, which contains polyunsaturated linolenic acid. The composition of the concentrate includes legumes, processed by special technology for the removal of anti-nutritional substances. Data on the effect of pre-starter compound feed containing the developed concentrate on the metabolism of nitrogenous substances in the body and some indicators of the neurohumoral link of the immunity of suckling pigs have been presented in the article. The animals had an intensive metabolism of nitrogenous substances. The total protein content in the blood serum was in the range of 65,53–65,73 g/l, and the ratio of albumins to globulins (coefficient A/G) was optimal – 1,55–1,67. The presence of a large amount of urea and glucose in the blood (3,05–3,72 and 6,60–5,80 mmol/l) has indicated that crossbred piglets had intensive synthetic processes associated with their growth. They contributed to the accumulation of M and G classes of immunoglobulins in the blood. The IgG content in the blood was 0,58–1,36 mg/ml, and IgM was 0,71–0,80 mg/ml. In terms of the amount of immunoglobulin G, the experimental group of piglets significantly exceeded the control group. Intestinal microbiocenosis in animals that have been received the new pre-starter compound feed was optimal. It has been dominated by positive microfloralactic acid and bifidum-bacteria, which inhibited the growth of pathogenic microflora, increasing the resistance of the piglets of the experimental group. Average daily gains in body weight were almost the same in both groups at the level of 253–254 g. Research results indicate that the new concentrate and pre-starter compound feed containing it had had the favorable effect on nitrogen metabolism and the immune status of the body of suckling piglets.


REPORTS ◽  
2021 ◽  
Vol 335 (1) ◽  
pp. 53-60
Author(s):  
V.G. Semenov ◽  
D.A. Baimukanov ◽  
S.G. Kondruchina ◽  
Kh.A. Aubakirov ◽  
T.N. Ivanova ◽  
...  

Productive insemination of cows against the background of heat detection or synchronization of the estrous cycle within a relatively short time after the previous calving is the main factor that determines the productivity and profitability of dairy cattle breeding. Many obstetric and gynecological diseases often occur in the first 2 months of lactation. Postpartum obstetric and gynecological diseases of cows impair their reproductive qualities, which leads to prolonged anestrus, decreased fertility, and depletion of the body. Inflammatory processes of the female reproductive organs are accompanied by enhanced stress. The research aims to prevent diseases of the postpartum period and realize the potential of the reproductive qualities of cows by activating the nonspecific resistance of the organism with the Prevention-N-B-S biological preparation. Based on the performed studies, a method for the prevention of obstetric-gynecological diseases of the postpartum period and an increase in the reproductive function of cows by increasing the nonspecific resistance of the body with the biological Prevention-N-B-S is proposed. The newly designed biological preparation was for the first time used in cows of the 2nd experimental group intramuscularly at a dose of 10 ml 35-30, 15-10, and 10-5 days before the expected calving time, and the animals of the 1st experimental group were injected with Dorogov's antiseptic stimulator of fraction 2 in combination with eleovitum in a ratio of 1:9 60 days before the scheduled calving dates. It was found that in cows of the 2nd trial group (28.8±0.56 days), the first estrus began 5.8 and 14.4 days earlier than in the mates of the 1st trial group (34.6±0.93 days) and control (43.2±1.64 days) groups. There was a decrease in the conception rate in cows of the 1st (1.8±0.24) and 2nd (1.4±0.36) trial groups, in comparison with the control (2.6±0.26). The service period in the 1st (64.6±1.62 days) and 2nd (57.8±1.50 days) experimental groups decreased compared to the control group (89.2±3.02 days). In the first sexual estrus in the control, 1st and 2nd experimental groups, 2, 4, and 6 cows have become fertile, respectively. The use of biological in the critical periods of cow pregnancy reduced the risks of uterine subinvolution, endometritis, and mastitis in the postpartum period, and also increased the reproductive qualities of cows. The most expressed corresponding effect was obtained when using the biological preparation Prevention-N-B-S.


2019 ◽  
Vol 20 (4) ◽  
pp. 387-397
Author(s):  
A. A. Ivanovsky ◽  
N. P. Timofeev ◽  
S. A. Ermolina

The effect of Phytoplus on weaned piglets was studied for the period of 50 days in 2018 and the influence of Algasol preparation on pregnant sows was investigated during 60 days in 2017. Phytoplus contains extracts from Rhaponticum carthamoides, Serratula coronate, and Filipendula ulmaria plants. It was administered daily, against the background of the control, into the diet of piglets aged from 40 to 90 days (n = 50 in the group): group No. 1 ‒ 0.3 g; group No. 2 ‒ 0.5 g; group No. 3 ‒ 1 gram per head per day. When Phytoplus was used, livability of piglets in all groups was 100%, the incidence in the experimental groups (2.3±0.3 heads) was 1.6-2.5 times lower than in the control group (5 heads). The live weight in the experimental groups (48,3±5,9 ‒ 51,4±7,5 кг) exceeded the result in the control group (41.8 kg) by 15-22%, and the intensity of the average daily weight gain by 24-33% (764,0±3,0 g ‒ 818,0±4,3 g against 616±2,2 g). Significant difference (P <0.05) was observed in piglets of the 3rd group, where AST increased, as compared with the beginning of the experiment, by 21.3% (from 15.9±0.6 to 19.3±0.5 units / l), and ALT by 12% (from 23.3±1.1 to 26.1 ± 2.1 units / l) and in the 2nd group where ALT increased by 46% (from 17.7±1.3 to 23.9±1.3 units /l). Algasol is a preparation from the extracts of brown algae and licorice roots. The experiment was carried out on nursing sows of large white breed pigs (2 groups of 48 animals each) when farrowing and before weaning piglets at 28 days of age. Sows of the experimental group were given Algasol daily at a dose of 50 ml per head. Algasol optimized their overall condition. The sows of the experimental group yielded 2% (572 pigs) more than in the control (561 pigs), the stillbirth decreased by 8% (23 vs. 25 animals). The death rate in the experiment was 2.1 times lower than in the control (17 vs. 8 goals), and livability was 98.5%. It has been established that Phytoplus and Algasol exert influence on the adaptive-compensatory reactions in the body of piglets and sows, that is proved by an increase in weight gain, livability of the young, and rise in the milk yield of the sows.


2021 ◽  
Vol 23 (103) ◽  
pp. 35-42
Author(s):  
V. B. Dukhnitskyi ◽  
I. M. Derkach ◽  
S. S. Derkach

The degree of the physiological development of the body of piglets in the first months after birth is important because, during this period, there is an active formation of many organism systems, including those that provide homeostasis. The resistance of piglets to diseases of various etiologies during life depends on the formation of the immune system. Our study aimed to assess the immune status of piglets born to sows that received injections of solutions of Iron(IV) clatrochelate and cyanocobalamin during pregnancy. To achieve this goal, two groups of newborn piglets-analogs were formed during their retention with suckling sows – control and experimental, 15 animals each. Piglets from 5 sows (3 from each) were injected into the experimental group, which were injected 10 ml of 10 % solution of Iron(IV) clatrochelate and cyanocobalamin solution twice during pregnancy (14 and 7 days before the expected farrowing) intramuscularly. Piglets of the control group, according to the traditional scheme of prevention of iron deficiency anemia on the second day of life, were administered irondextran drug uniferon (200 mg of iron (III) per injection). Piglets and sows were observed for two months; for studies of the content of immunoglobulins in the piglets' serum, the blood samples were taken at 1, 5, 12, and 30 days of life. During the experiment, the indicators of the content of immunoglobulins of classes G, A, M in the serum of piglets of the experimental group and the dynamics of their changes within 30 days after birth did not differ from the control. Two injections of 10 % solution of Iron(IV) clatrochelate in a dose of 10 ml in combination with cyanocobalamin injections at a dose of 500 mg of active substance to pregnant sows 14 and 7 days before the expected farrowing did not adversely affect the immune status of newborn piglets. Furthermore, this has a high prophylactic effect against iron deficiency anemia.


2020 ◽  
pp. 15-18
Author(s):  
Inna R. Kilmetova ◽  
◽  
Igor A. Rodin ◽  
Nazira I. Khayrullina ◽  
Nikolay G. Fenchenko ◽  
...  

Summary. The disbalanced feeding and the uneven distribution of micro- and macroelements in the environment leads to a trace element, in particular hypomelanosis. To accelerate the growth and preservation of young farm animals include in the diet of various biological additives and drugs, which include selenium. For stimulation of weight gain in the livestock industry, as well as for the prevention and treatment of pathological processes in addition to micro - and macrouse amino acids, primarily methionine. The aim of this work was to study the influence of composition of DAFS-25+Polizon on morpho-biochemical parameters of blood and functional state of the liver in fattening bulls of black-motley breed in the conditions of the Republic of Bashkortostan. Experiments using were conducted on bull-calves of black-motley breed of the properties in the properties age from 6 to 15 months. The first experimental group during the experiment was additionally given the composition of DAFS-25+Polizon at a dose of 2 mg/kg, the animals of the control group received a standard diet. To assess the impact of the composition DAFS-25+Polizon on metabolism cattle studied morphological and biochemical indicators of blood and conducted histological examination of the liver. It is established that the use of the composition of DAFS-25+Polizon at a dose of 2 mg/kg increases the number of erythrocytes and hemoglobin in the experimental group and reduces the amount of white blood cells. The serum content of total protein, phosphorus and calcium increases in the group of experimental animals. Microscopic examination of the liver revealed no changes in the structure of the organ and hepatocytes in the experimental group, whereas in the control group hemodynamic disorders and dystrophic changes in liver cells were observed. Thus, the use of the composition DAFS-25+Polizon at a dose of 2 mg/kg of live weight in fattening bulls black-and-white breed contributes to the increase of redox processes in the body, stimulation of metabolism, prevent the development of liver disorders of cellular mechanisms of metabolism, optimizes the structure of the liver, which generally provides higher productivity.


Author(s):  
M. Grigoryev ◽  
N. Chernogradskaya

Animals imported to Yakutia from other regions fall into feeding and housing conditions that differ from their usual agro-climatic conditions, which causes the problem of their adaptation. Yakutia is one of the areas where the feed of its own production has a lack of basic minerals, which affects the health and productivity of animals, which has an additional negative influence on the adaptation process of imported cattle. In order to replenish macro- and microelements in the body, it is necessary to introduce various local mineral feed additives, premixes, and use mineral salts into the ration. Under the conditions of Central Yakutia the influence of local mineral feed additives (zeolite of the Khonguruu Deposit in the Suntar district and sapropels of local lakes) containing macro- and microelements that increase the meat productivity of steers has been studied. As a result of the research the influence of local mineral feed additives on meat productivity and physiological condition of Hereford steers has been established. Influence of zeolite and sapropel in a complex with mineral salts on digestibility of nutrients of the ration and use of nitrogen, calcium and phosphorus by experimental animals has been defined. The influence of feed additives on the quality of meat products has been studied. The results have shown that the use of local mineral feed additives in the composition of ration of steers during the fattening period at a dose of 0,7 g zeolite per 1 kg of live weight of the animal and 200 g of sapropel in combination with 10 g of mineral salts contributed to the gain in live weight, carcass weight, carcass yield, yield of edible parts, and economic efficiency of fattening. For the entire period of the experiment the gain in live weight in groups was: in control group 2222,9 kg, in the 1st experimental group 2396,2 kg, in the 2nd experimental group 2471,1 kg. The total economic effect for the 1st experimental group for the period of experiment amounted to 44,3 thousand Rubles, for the 2nd experimental group 78,0 thousand Rubles or per 1 head/day 14,06 and 24,76 Rubles, respectively.


Author(s):  
S. Grikshas ◽  
N. Kulmakova ◽  
K. Spitsyna ◽  
A. Dar’in ◽  
T. Mittelshtein

Mycotoxins have been formed in feed are secondary metabolites of fungi and are quite stable substances that have teratogenic, mutagenic and carcinogenic effects. An effective way to combat mycotoxins in feed is the use of feed additives that adsorb toxins, prevent their absorption in the gastrointestinal tract of the animal and are excreted from the body. The infl uence of coconut enterosorbent Shelltic Es on fattening and meat qualities of young pigs has been studied. It has been found in the process of fattening that the highest feed digestibility was in pigs of the experimental group, in the diet of which enterosorbent has been added. The results of studies of the chemical composition and technological properties of pork have been provided. The positive eff ect of enterosorbent on precocity, absolute average daily gain of live weight and reduction of feed expenditures per 1 kg of gain has been revealed. In pigs from the experimental group the average thickness of the fat was higher and the area of the “muscle eye” was lower compared with animals of the control group, which indicates that higher rates of carcass yield have been obtained due to faster accumulation of fat tissue. The weight of internal organs of pigs indicates the intensity of metabolic processes in the body. In experimental animals the weight of the lungs was 0,1 kg lower, and the liver and heart were higher by 0,13 and 0,01 kg, respectively. Enterosorbent had no effect on the content of vitamins in the liver of pigs of the compared groups. The content of impurities of organochlorine toxicants and toxic elements in the meat and liver of animals of the experimental group was lower than that of control analogues. Therefore, the use of enterosorbent Shelltic Es promotes more active excretion of them from the body.


Author(s):  
Xiaona Yang ◽  
Yang Ji ◽  
Da Ke ◽  
Fang Li

Aerobics is a beautiful and rhythmic sport. In recent years, aerobics has developed rapidly, and has become one of the essential courses of physical education in Colleges and universities. But the existing aerobics courses in Colleges and universities are less involved in the training process of action strength. Lack of strength quality is a common shortcoming of Aerobics Athletes in Colleges and universities. Therefore, this paper puts forward the methods and means of action strength training in aerobics training. According to the characteristics of competitive aerobics, combined with the traditional training strategy of action strength, this paper formulates the method of action strength training suitable for College Aerobics athletes. In order to further verify the effectiveness of this method, this paper takes a university aerobics team as an experimental sample to carry out a comparative analysis of the effect of action strength training. Relevant studies have proved that core strength training has the following effects on aerobics players: 1. Helps the calisthenics team member stabilize the trunk, improve the body's control ability and balance ability. 2. It is conducive to completing difficult movements of calisthenics with high efficiency and low energy consumption. 3. Helps the aerobics team member to enhance the special strength. 4. It can effectively prevent sports injuries. 5. It can improve the direction change and displacement speed of the body. 6. It can improve the energy output of the core strength to the limbs and other muscle groups during movement, so that the movement can be completed more perfectly.According to the results of the experiment, the explosive force and endurance of the experimental group after 8 weeks of training have been significantly improved, and formed a more obvious difference with the control group. The aerobics training method developed in this paper can be divided into two stages: the initial stage and the middle stage, which can better meet the students with different physical qualities. At the end of this paper, a fast strength training method is introduced, and the characteristics of different training methods are analyzed.


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