scholarly journals Effect of Dorsomorphin Homolog 1 (DMH1) against Diabetic Dyslipidemia in Streptozotocin-induced Diabetic Rats

2021 ◽  
pp. 246-256
Author(s):  
Abdulaziz M. Alzahrani ◽  
Mai A. Alim A. Sattar Ahmad ◽  
Basma T. Alharthy
Keyword(s):  
Bone Morphogenetic Proteins ◽  
Wistar Rats ◽  
Fasting Blood Glucose ◽  
Experimental Studies ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Smad Pathway ◽  
Blood Samples ◽  
Adverse Cardiovascular Outcome

Dyslipidemia is usually observed in both types of diabetes and, particularly, “atherogenic dyslipidemic triad” is strongly linked to a higher risk of adverse cardiovascular outcome. On the other hand, bone morphogenetic proteins (BMP) are a group of wide variety of proteins which were found overexpressed and implicated in contribution and acceleration of atherosclerotic calcification. So, the present study aimed to assess effect of DMH1, a selective BMP inhibitor, in a rat model of diabetic-induced dyslipidemia. Methods: STZ-induced diabetes in Wistar rats was used as a model to assess the antihyperlipidemic effect of DMH1(5mg/kg) for a period of 8 weeks. Rats were divided intonormal control (C=10), diabetic control (DC=10), diabetic+vehicle (DV=10) and diabetic DMH1-treated rats (DT=10). Fasting blood glucose (FBG) level was measured on weekly bases. Then, at the end of the experiment, rats were anesthetized and blood samples were collected for the determination of total cholesterol (TC), triglyceride (TG), LDL and HDL levels using the appropriate ELISA assay. Results: FBG levels for all diabetic groups were significantly high, during the experiment period, compared to the control (P< 0.001). While dyslipidemia was remarkable in the diabetic non-treated groups, DMH1 treatment showed a significant decrease in TC (P< 0.001), TG (P< 0.05) and LDL levels (P< 0.001) compared to the non-treated groups (DC & DV). Concurrently, HDL levels for DT group were significantly increased compared to DC or DV groups (P< 0.01). Conclusion: The present experiment showed that DMH1 possessed encouraging activityagainst dyslipidemia in STZ-induced diabetic rats. Our results are promoting for more interest and investigation regarding antihyperlipidemic effect of DMH1 and BMP/Smad pathway in further experimental studies.

Effects of dietary fiber on glycemia and lipid profile in aging diabetic rats

2021 ◽  
Vol 11 (4) ◽  
pp. 362-368
Author(s):  
Khayra Mebarek ◽  
Meryem Bensalah ◽  
Samira Bouanane ◽  
Fatima Zohra Baba Ahmed ◽  
Nesrine Samira Karaouzene ◽  
...  
Keyword(s):  
Lipid Metabolism ◽  
Dietary Fiber ◽  
Wistar Rats ◽  
Control Diet ◽  
Plasma Lipid ◽  
Diabetic Control ◽  
Physiological Effect ◽  
Diabetic Rats ◽  
Lipolytic Enzyme ◽  
Pure Cellulose

Dietary fiber is a group of food components which is the subject of many studies on several aspects of human health. Recent research demonstrate that dietary fiber intake is associated with reduced diabetes risk. The aim of the present work was to test the effect of dietary fiber such as cellulose and mucilage on disorders of lipid metabolism induced by experimental diabetes in the aged Wistar rats. Diabetes was induced by intraperitoneal injection of streptozotocin. Aging male Wistar rats diabetic and control rats were fed highly-pure-cellulose-mucilage-enriched (HPCME) diet or control diet for 2 months. At the end of study, blood samples and tissue are collected for de-termination of biochemical parameters (glucose, total cholesterol, triglycer-ides and lipoproteins) and lipases activities. 2 months of HPCME diet intake by diabetic aged rats improves diabetic control, induced a decrease of body weight, a reduction of plasma lipid concentration, lower blood-glucose and a significant decrease in expression of pathway lipolytic enzyme activities va-lues witch decrease the prevalence of the specific disorders of diabetes. This study suggests that dietary fiber (HPCME), has an important physiological effect on glucose and lipid metabolism during aging which reduces the risk of developing complications of diabetes.

scholarly journals Antidiabetic potential of the leaf extract of Centella asiatica in alloxaninduced diabetic rats

2016 ◽  
Vol 4 (1) ◽  
pp. 51-59 ◽  
Author(s):  
Talha Bin Emran ◽  
Mycal Dutta ◽  
Mir Muhammad Nasir Uddin ◽  
Aninda Kumar Nath ◽  
Md Zia Uddin
Keyword(s):  
Body Weight ◽  
Leaf Extract ◽  
Total Cholesterol Level ◽  
Serum Insulin ◽  
Fasting Blood Glucose ◽  
Centella Asiatica ◽  
Liver Glycogen ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Diabetic Rat

The study was designed to evaluate the glucose and cholesterol lowering effect of the aqueous extract of Centella asiatica leaf using the alloxan-induced diabetic rats and compared the activity with diabetic control and antidiabetic drug (Glibenclamide). Leaf extract (50 mg/kg) of C. asiatica and Glibenclamide were administered to normal and experimental diabetic rats for the duration of 10 days. In the alloxan-induced diabetic rat model, C. asiatica extract (50 mg/kg) significantly (p < 0.05) lowered the fasting blood glucose level as well as the total cholesterol level. Serum insulin levels were not stimulated in the animals treated with the extract. In addition, changes in body weight, serum lipid profiles and liver glycogen levels assessed in the extract treated diabetic rats were compared with diabetic control and normal animals. Significant results (p < 0.05) were observed in the estimated parameters. Surprisingly, body weight was increased significantly (p < 0.05) in the C. asiatica treated diabetic group. Phytochemical screening showed the presence of alkaloids, flavonoids, glycosides, steroids and tannins in significant amountsJahangirnagar University J. Biol. Sci. 4(1): 51-59, 2015 (June)

scholarly journals Hypoglycaemic and Hypolipidemic Effects of Treculia africana Aqueous Leaves Extract in Alloxan Induced Diabetic Rats

2018 ◽  
pp. 1-9
Author(s):  
Idris A. Kankara ◽  
Gayus A. Paulina ◽  
M. Aliyu
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Total Cholesterol ◽  
Ldl Cholesterol ◽  
Fasting Blood Glucose ◽  
Hdl Cholesterol ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Albino Rats ◽  
Treculia Africana

This study investigated the hypoglycaemic and hypolipidaemic effects of Treculia africana plant used in Nigeria as medicinal plant. Diabetes mellitus was induced by a single dose intraperitoneal injection of alloxan 150 mg/kg body weight. Twenty five (25) male albino rats were divided into five groups, five (5) rats per group; normal control, diabetic control and diabetic groups treated with aqueous leaves extract of 200,400 and 800 mg/Kg body weight respectively for 21 days orally. The effects of the extract on some biochemical parameters were evaluated; fasting blood glucose level was assayed using glucose oxidase method, total cholesterol and HDL –cholesterol were assayed using enzymatic method while LDL- cholesterol was determined by Friedewald equation. The results showed that, extract significantly (p<0.05) decrease the elevated fasting blood glucose levels, total cholesterol, triglyceride and LDL- cholesterol when compared with the diabetic control rats. The extract also caused significant (p<0.05) increased in HDL –cholesterol and body weight when compared with diabetic control rats. Aqueous leave extract of Treculia africana possess hypoglycemic effect and the most effective dose was 800 mg/Kg body weight in amelioration of hyperglycaemia and most all toxicity effects of alloxan on lipid profile.

scholarly journals Extracts of Guiera senegalensis J.F. Gmel (Combretaceae) Galls Ameliorates Streptozotocin-Induced Diabetes Mellitus Rats Status

2019 ◽  
pp. 1-11
Author(s):  
Pierre Alexandre Eric Djifaby Sombié ◽  
Rahman Hafizur ◽  
Martin Kiendrébéogo ◽  
Muhammad Iqbal Choudhary ◽  
Odile Germaine Nacoulma
Keyword(s):  
Blood Glucose ◽  
Blood Glucose Level ◽  
Antioxidant Status ◽  
Wistar Rats ◽  
Methanol Extract ◽  
Insulin Level ◽  
Diabetic Rats ◽  
Antidiabetic Activity ◽  
Guiera Senegalensis

Background: A good number of medicinal and dietary plants are used for diabetes treatment in Burkina Faso. Aim of the Study: The present study aimed to investigate the antidiabetic activity of Guiera senegalensis galls extracts and its potential mechanisms in streptozotocin-induced diabetic rats. Methodology: The methanol extract was administered by gavage to healthy Wistar rats for the determination of toxicity, to normal and diabetic Wistar rats for the determination of glucose reduction level, lipid profile, insulin level and glycaemic parameters in serum. The histology and immunohistochemistry of the pancreas were also determined. Results: The acute toxicity results showed that the medium lethal dose (LD50) of the methanol galls extract of Guiera senegalensis is greater than 2000 mg/kg body weight in rats. Guiera senegalensis methanolic extract (250 mg/kg) and the tolbutamide (100 mg/kg) recorded a significantly (p < 0.05) lower level of triglyceride compared to the diabetic group. The methanol extract (250 and 500 mg/kg pc) significantly (p < 0.05) decreased the blood glucose level and increased the serum insulin level in diabetic rats. Interestingly, improved ß-cell function and antioxidant status were also observed in G. senegalensis-treated diabetic rats when compared to tolbutamide-treated diabetic rats. Conclusion: These data showed direct evidence that G. senegalensis has antidiabetic activity by decreasing blood glucose level, improving insulin secretion and β-cell functions and modulating antioxidant status.

scholarly journals Modulations of Some Carbohydrate Metabolic Enzymes by Aqueous and Ethanol Buchholzia coriacea Seed Extract in Alloxan Induced Diabetic Rats

2018 ◽  
pp. 1-9
Author(s):  
K. O. Gbagbeke ◽  
A. O. Naiho ◽  
P. R. C. Esegbue ◽  
M. O. Odigie ◽  
M. A. Omoirri
Keyword(s):  
Blood Glucose ◽  
Aqueous Extract ◽  
Fasting Blood Glucose ◽  
Ethanol Extract ◽  
Metabolic Enzymes ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Total Carbohydrate ◽  
Adult Rats ◽  
Ethanol Extracts

Characterised by abnormal increase in blood glucose level, Diabetes is a metabolic disorder that is associated with complications in carbohydrate, protein and fat metabolism. In recent times, medicinal herbs have been implicated in traditional medical practice for the treatment of this ailment. Studies have shown that Buchholzia coriacea seed possesses some anti-hyperglycemic properties that may be useful in the management of diabetes. To this point, present study investigated the effect(s) of oral administration of aqueous and ethanol extracts of Buchholzia coriacea on some carbohydrate metabolism parameters in normal and alloxan-induced diabetic rats. Forty (40) adult rats of both sexes were randomly assigned into two groups (normoglycemic and hyperglycemic). While group 1 (normoglycemic) had normal control, metformin, aqueous extract (250mg/kg) and ethanol extract (250 mg/kg) treated sub-groups respectively, Group 2 (hyperglycemic) contained the diabetic control, metformin, aqueous extract (250 mg/kg), and ethanol extracted (250mg/kg) treated sub-groups dosed daily by oral gavage for 14 days. At the end of the treatment, rats were euthanized via cervical dislocation; blood samples were collected by cardiac puncture for statistical analysis. One-way analysis of variance (ANOVA) revealed that dosing with extracts had insignificant effect(s) on body weight of rats. Fasting Blood Glucose (FBG) levels were elevated before and after extracts administration. Metformin, aqueous and ethanol extracts significantly reduced (p<0.05) FBG levels. Also, compared with control, total carbohydrate, liver glucose, glycosylated haemoglobin, Lactate Dehydrogenase, Isocitrate dehydrogenase, MDH, SDH, 6-phosphogluconate dehydrogenase, G6PD and CcO activities were significantly reduced (p<0.05) in diabetic treated rats. Buccholzia Coriacea was therefore seen to pose hypoglycemic and glycolytic effects, regulating activities of carbohydrate metabolic enzymes. Apparently, there is a scientific merit in the use of the extract in the management of diabetes.

scholarly journals The Effect of Belimbing Wuluh (Averrhoa Bilimbi L.) and Red Guava (Psidium Guajava L.) Extract on Fasting Blood Glucose Levels of Diabetic Wistar Rats Model

2019 ◽  
Vol 5 (2) ◽  
pp. 57
Author(s):  
Yesi Warisman ◽  
Arinda Lironika Suryana ◽  
Zora Olivia
Keyword(s):  
Blood Glucose ◽  
Blood Glucose Level ◽  
Glucose Level ◽  
Wistar Rats ◽  
Fasting Blood Glucose ◽  
Diabetic Rats ◽  
Control Group ◽  
Standard Diet ◽  
Glucose Levels ◽  
Blood Glucose Levels

Diabetes mellitus is a metabolic disease with special characteristic which increased blood glucose level (hiperglicemic), its happens because of the abnormality of insulin secretion, insulin mechanisme or both of them. Belimbing wuluh and red guava extract can become alternative functional drink that contain rich of vitamin C to help controlling blood glucose level. The aimed of this study was to determined the effect of belimbing wuluh and red guava extract on blood glucose levels of Rattus novergicus strain Wistar diabetic model. This research was an true experimental study with a randomized post-test control group design. Twentyfour (24) male wistar strain rats weighing 200-250 grams, aged 2-3 months, were divided into 4 groups. The control group (K-) non-diabetic rats, the control group (K+) diabetic rats given standard diet, and the treatment group diabetic rats given standard diet and belimbing wuluh and red guava extract intervetion doses 10,7 ml/day (P1) and 21ml/day (P2). Measurement of pretest posttest fasting blood glucose levels was carried out using biolyzer. The data were analyzed by One Way Anova test. The results showed that there were significant differences in delta pretest and posttest fasting blood glucose levels between all groups. Blood glucose levels decreased at 55.6%. In conclusion, belimbing wuluh and red guava extract 21ml/day most effectively decreased fasting blood glucose levels in diabetic wistar rats moldel.   Keywords: belimbing wuluh, blood glucose level, diabetes mellitus, red guava

scholarly journals ANNONA MURICATA LINN. ETHANOLIC LEAF EXTRACT AMELIORATES REPRODUCTIVE COMPLICATIONS IN STREPTOZOTOCIN-INDUCED DIABETIC WISTAR RATS

2020 ◽  
Vol 18 (1) ◽  
pp. 166-175
Author(s):  
O. E. ADELEYE ◽  
N. A. ABOAJAH ◽  
A. I. ADELEYE ◽  
E. A.O. SOGEBI ◽  
F. M. MSHELBWALA ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Sperm Count ◽  
Fasting Blood Glucose ◽  
Diabetic Rats ◽  
Male Rats ◽  
Annona Muricata ◽  
Major Health Problem ◽  
Ethanolic Leaf Extract

Diabetes mellitus is an endocrine and metabolic disorder of humans and animals characterized by hyperglycemia and low blood insulin levels or insensitivity of target organs to insulin and it’s a major health problem affecting patient’s quality of life due to its many complications. Infertility is one of the major secondary complications in diabetes. Although numerous drugs have been used for intervention studies on diabetes-induced infertility worldwide, there are currently no treatments for diabetes associated infertility in humans. This study was performed to investigate the effects of Annona muricata ethanolic leaf extract (AMELE) on fertility of male diabetic rats and levels of blood glucose. Twenty male Wistar rats (150-200g) were randomly distributed into 4 groups (n=5) treated thus: CTRL (control), DNT, DT1 and DT2 (diabetic, single intraperitoneal injection, streptozotocin, 60 mg/kg). Group DT1 and DT2 received AMELE orally at 100 mg/kg and 200 mg/kg respectively daily for fourteen days. Data were analysed using ANOVA at α0.05. The animals were sacrificed after 2 weeks via thiopental injection and testicular weights were recorded. Fasting blood glucose was determined using a digital glucometer. Sperm count, motility, viability and morphology were assessed microscopically. Testes were histologically evaluated. The results showed that oral administration of AMELE at 100 mg/kg and 200 mg/kg to diabetic male rats for fourteen days significantly decreased blood glucose level and also ameliorated diabetes-induced decreases in sperm functions in streptozotocin-induced diabetic male rats.    

scholarly journals Polygonatum sibiricum extract exerts inhibitory effect on diabetes in a rat model

2021 ◽  
Vol 18 (7) ◽  
pp. 1493-1497
Author(s):  
Lai-zeng Yu ◽  
Xue-peng Zhang ◽  
Ying-xin Wang
Keyword(s):  
Lipid Peroxidation ◽  
Blood Glucose ◽  
Fasting Blood Glucose ◽  
Insulin Level ◽  
Serum Levels ◽  
Inhibitory Effect ◽  
Diabetic Control ◽  
Negative Control ◽  
Diabetic Rats ◽  
Dependent Manner

Purpose: To investigate the effect of Polygonatum sibiricum extract (PSE) on streptozotocin-induced diabetic rats. Methods: PSE was obtained by steeping the dried Polygonatum sibiricum in water at 60 oC three times, each for 1 h, before first drying in an oven at 100C and then freeze-drying the final extract, thus obtained. Diabetic model rats were prepared by a single intraperitoneal injection of a freshly prepared solution of streptozotocin (50 mg/kg). The rats were randomly divided into 6 groups of ten rats each: negative control, normal control, reference (glibenclamide1 mg/kg) as well as PSE groups, (35, 70 and 140 mg/kg). Blood glucose and plasma insulin levels were measured to determine antihyperglycemic effect. Oxidative stress was evaluated in liver and kidney by their antioxidant markers, viz, lipid peroxidation (LPO), superoxide dismutase (SOD), reduced glutathione (GSH), glutathione peroxidase (GPx) and catalase (CAT). Blood serum levels of creatinine and urea were determined in both diabetic control and treated rats. Results: Compared with diabetic rats, oral administration of PSE at a concentration of 120 mg/kg daily for 30 days showed a significant decrease in fasting blood glucose (118.34 ± 3.29 mg/dL) (p < 0.05) and increased insulin level (12.86± 0.62 uU/mL, p < 0.05). Furthermore, it significantly reduced biochemical parameters (serum creatinine, 0.83 ±0.21 mg/dL, p < 0.05) and serum urea (43.26±1.42 mg/dL, p < 0.05). Conclusion: The results suggest that PSE may effectively normalize impaired antioxidant status in streptozotocin-induced diabetes in a dose-dependent manner. Thus, PSE has a protective effect against lipid peroxidation by scavenging free radicals, restoration of insulin function, and reduction of the incidence of complications.

Attenuation of Alloxan-induced Low-grade Inflammation by Persea Americana Polyphenolic Peel Extract in Male Wistar Rats

2021 ◽  
Author(s):  
OLAREWAJU MICHAEL oluba ◽  
Daniel O. Abasiri ◽  
Babatunde Ibitoye ◽  
Samuel Ojeaburu ◽  
George Eidangbe ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Wistar Rats ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Persea Americana ◽  
Low Grade ◽  
Distilled Water ◽  
Management Of Complications ◽  
Low Grade Inflammation ◽  
Peel Extract

Abstract Background: Based on the conceived causal relationship that exist between oxidative stress, hyperglycemia and low‐grade inflammation, this study was hypothesized to evaluate how treatment with polyphenolic peel extract of avocado pear (APPE), with established antioxidant activity, may be deployed in the management of complications arising from diabetes. Methods: Twenty adult male alloxan-induced diabetic Wistar rats randomly assigned to five groups (n = 5) designated: diabetic control (DC), treated with distilled water; and 25, 50, 100 and 200 mg/kg APPE, respectively. A sixth group comprising of five normal rats administered distilled water served as normal control (NC). Results: Following a 21-day single daily oral administration of APPE, remarkable improvements in body weight and blood glucose concentration were noted in diabetic rats especially at 200 mg/kg compared to DC. A dose-related significant reductions in glycated hemoglobin, interleukins (1β and 6), tumour necrosis factor-α (TNF-α) levels in diabetic rats treated with APPE compared to DC. Conversely, serum insulin and interleukin-10 concentrations were significantly elevated in a dose-related fashion in APPE administered diabetic rats compared to DC. Unlike the intestine and heart which were spared severe morphological treatment due to alloxan intoxication, diabetic control rat and those on low dose of the extract showed varying degree of interface hepatitis and glomerulosclerosis. However, diabetic rats on 200 mg/kg APPE were protected from the damaging effects of diabetes mellitus on the liver and kidney.Conclusion: APPE abrogated the oxidative stress-induced hepatic and nephrotic damage in diabetic rats.

scholarly journals Glycaemic, Lipidaemic and Antioxidant Profiles of Alloxan-Induced Diabetic Wistar Rats Treated with Glibenclamide and Aqueous Extract of Gongronema latifolium (Benth)

2016 ◽  
Vol 8 (4) ◽  
pp. 408-413
Author(s):  
Patrick Emeka ABA
Keyword(s):  
Wistar Rats ◽  
Combined Therapy ◽  
Low Density Lipoprotein ◽  
Fasting Blood Glucose ◽  
Density Lipoprotein ◽  
Study Groups ◽  
Oral Route ◽  
Diabetic Rats ◽  
Glucose Levels ◽  
Male Wistar Rats

The current study investigated the ameliorative effects of combined therapy of glibenclamide and G. latifolium (GL) on several biochemical parameters of alloxaized Wistar rats. Thirty adult male Wistar rats assigned into 5 groups of 6 rats each were used for the study. Groups 2-5 were intraperitoneally injected with 160 mg/kg of alloxan monohydrate and upon establishment of diabetes (Fasting Blood Glucose (FBG) ≥ 126 mg/dl) were treated with 10 ml/kg distilled water (DW), 2 mg/kg glibenclamide, 200 mg/kg GL and 2 mg/kg glibenclamide and 200 mg/kg GL respectively. Rats in group 1 were not made diabetic and served as normal control. All the treatments were realized through daily oral route using gastric tube, for 21 days. Results indicated that the treatment of diabetic rats with a combination of glibenclamide and GL significantly reduced the elevated glucose levels, cholesterol, triacylglycerol, low density lipoprotein and malondialdehyde levels, along with increases in the high density lipoprotein, glutathione values and catalase activities, when compared to diabetic untreated group. It was concluded that the combined therapy of glibenclamide and GL showed superior antihyperglycemic, hypolipidaemic and antioxidant effects compared to either of them used alone.

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