ABSTRACTObjectivesTo investigate associations between occupational physical activity (OPA) and all-cause mortality.MethodsFrom baseline (2006-2010), 452,884 UK Biobank participants (aged 40-69 years) were followed for a median 11.1 (IQR: 10.4-11.8) years. OPA was categorised by cross-tabulating degree of manual work and walking/standing work amongst those in paid employment (n=264,424), whereas categories of occupational status were used for those not in paid employment (n=188,460). Cox proportional hazards models were used to estimate hazard ratios (HR) and 95% confidence intervals (CI) for all-cause mortality by occupational category, and for working hours/week and non-occupational physical activity stratified by occupational category.ResultsDuring 4,965,616 person-years of follow-up, 23,310 deaths occurred. Compared to those in sedentary jobs, retirement was associated with lower mortality in women (HR=0.74, CI:0.68-0.81) and men (HR=0.85, CI:0.79-0.92), whereas unemployment was associated with higher mortality in men (HR=1.26, CI:1.10-1.45). There was no evidence of mortality differences by OPA category within the working population. Working <35 hours/week versus 35-40 hours/week was associated with lower mortality in both women (HR=0.86, CI:0.79-0.93) and men (HR=0.81, CI:0.75-0.88), with no interaction by OPA. Non-occupational physical activity was associated with lower mortality in both women (HR=0.90 per 5 kJ/day/kg, CI:0.84-0.96) and men (HR=0.88 per 5 kJ/day/kg, CI:0.84-0.92), with no interaction by OPA.ConclusionWork classified as having higher levels of OPA may not be as active as reported, or the types of physical activity performed in those jobs are not health-enhancing. Irrespective of OPA category or employment status, non-occupational physical activity appears to provide health benefits.SUMMARY BOXWhat are the new findings?Retirement was associated with lower all-cause mortality, compared to having a sedentary job in both men and women but unemployment was only associated with higher mortality in men.There were no differences in mortality between categories with different levels of self-reported OPAPhysical activity outside of work was associated with lower hazard of all-cause mortality and there was no interaction with occupational physical activity, indicating similar benefits across different jobs types.How might it impact on clinical practice in the future?Health professionals should be aware that occupations assumed to involve more physical activity may not be as active as reported, or the types of physical activity performed in those jobs may not be health-enhancing.Physical activity during leisure-time should continue to be recommended to adults of all paid and unpaid occupational categories.