Detection of Babesia spp. in High Altitude Cattle in Ecuador, Possible Evidence of the Adaptation of Vectors and Diseases to New Climatic Conditions

Background: Babesia species are intraerythrocytic protozoa, distributed in tropical and subtropical areas of the world, causing anemic diseases in many animals, including cattle. This disease, called babesisosis, is transmitted from one animal to another through ticks (Tick Borne-Disease or TBD). On the other hand, Ecuador has a tropical climate that allows the development of the vector Rhipicephalus microplus, and therefore favors the transmission of Babesia spp. in cattle. Methods and principal findings: We determined the presence of Babesia spp. by PCR using 18s ribosomal gene as target (18s PCR) in 20 farms in the area of El Carmen (zone below 300 m above sea level) and 1 farm in Quito (2469 m.a.s.l.). In addition, we analyzed parameters such as age, sex, and packed cell volume (PCV) as explanatory variable associated with the disease. Results: The 18s PCR test showed that 18.94% (14.77% Babesia bovis and 4.17% Babesia bigemina) and 20.28% (14.69% B. bovis and 5.59% B. bigemina) of the cattle were positive for Babesia spp in farms sampled in El Carmen and in Quito, respectively. Age influenced the presence of animals positive for Babesia spp., but sex and PCV did not. The phylogenetic analysis of sequences showed 4 isolates of B. bovis and 3 isolates of B. bigemina in the 2 study zones, with similarities between 99.73 and 100% with other sequences. One B. bovis isolate was similar in the zone of El Carmen and Quito. Conclusion and significance: This work is the first molecular characterization of B. bigemina and B. bovis in Ecuador, and it is also the first evidence of Babesia spp. in cattle in the area of Quito at an altitude of 2469 m.a.s.l., being the highest altitude reported for animals with babesiosis and for the tick R. microplus. Climatic factors as well as mobility of tick-carrying animals without any control allow the presence of Babesiosis outbreaks in new geographical areas.

Development of a Kriging Assessment Framework and Impact Pathway for Researching Contamination With Potentially Toxic Elements in Urban Landfill Soil

Landfill post-closure with contaminant concentration in soil below permissible limit assessed at limited spot does not represent the contamination issue. Assessment limit to professionals also does not gives a potential of change to practice constant assessment to a wider context of assessor - citizen living nearby - as a collaborative effort to sustain a safe environment. Therefore sizeable, qualitative, and cost-effective analysis of the concentrations of contaminants is needed and this work recommends kriging assessment and the logical impact pathway framework as factors of change in landfill aftercare management. The kriging framework is developed utilising lead (Pb) and chromium (Cr) data from inductively coupled plasma mass spectrometry (ICP-MS) analysis. The development of the kriging framework is conducted based on the observation of censored data from ICP-MS analysis. The estimation analysis involves the analysis of ordinary kriging with regression analysis, showing the interpolation of spatial correlation and regression error. Hence, ordinary kriging with regression of the variable of interest, i.e., Pb, using the data of the explanatory variable, i.e., Cr, is inappropriate. Further investigation with the utilisation of guess-field kriging analysis hypothetically exposed a potential contaminated area using an existing but limited number of explanatory variables; although, guess-field kriging may possibly result immense uncertainty at the area where the explanatory variable does not exist. Besides, this work anticipated outcomes in societal impact and sustainability practices from the proposed kriging framework by recommending a logical impact pathway. The development of the kriging framework and impact pathway reassure the necessary actions to be executed by responsible parties and act as the stimulus of a wider spectrum of improvement initiatives to oversee real issues, such as the time of occurrence, and to prevent negative impacts on the environment and humans.

The association of workplace smoke-free policies on individual smoking and quitting-related behaviours

Background Our study aims to provide information about workplace smoke-free (SF) policy coverage in mainland China and to assess the relationship between workplace SF policies and secondhand smoke (SHS) exposure, current smoking, smoking harm awareness and quitting intention among smokers. Method Data from the 2018 Asia Best Workplace Mainland China programme were used to address these aims. This cross-sectional study included 14,195 employees from the 2018 survey and 14,953 employees from the 2019 survey. Logistic regression with year-fixed effects was applied to these data. The dependent variables were SHS exposure, smoking or smoking harm awareness. The explanatory variable was the SF workplace policy. Results A total of 21,275 participants (73.0%) reported working under SF policies. The overall prevalence of smoking and SHS exposure were 20.3% and 52.5%, respectively. The workplace SF policy was significantly associated with lower SHS exposure (OR: 0.48, 95% CI: 0.45–0.51), lower current smoking employees (OR: 0.81, 95% CI: 0.76–0.87) and higher awareness of smoking harm (OR: 1.76, 95% CI: 1.61–1.91). However, workplace SF policy was not significantly associated with quitting intention (OR: 0.99, 95% CI: 0.84–1.16). Conclusion Our study identified that although most companies had established workplace SF policies, the overall prevalence of SHS exposure remained very high. Workplace SF policy is associated with lower SHS exposure, lower overall current smoking and higher awareness of smoking harm. These findings provide valuable evidence to promote such policies in all workplaces.

Fish morphology and passage through velocity barriers. Experience with northern straight-mouth nase (Pseudochondrostoma duriense Coelho, 1985) in an open channel flume

Knowing the relationship between size, morphological traits and swimming performance of fish is essential to understand the swimming capacity to successfully surpass these obstacles and the selective pressure that barriers in rivers and streams could exert on fish. Northern straight-mouth nase, an endemic potamodromous cyprinid fish species from the Northwest of the Iberian Peninsula, was selected to carry out volitionally swimming performance experiments in an open channel against three different flow velocities, using telemetry and video cameras. The use of thin-plate spline, on 10 landmarks, evidenced unknown patterns linked to velocity barriers. At lower flow velocity, size is the main factor explaining the swimming performance; thus, large individuals swim up more efficiently. In contrast, at high flow velocities, shape becomes the essential explanatory variable; thereby, streamlined body shapes with a higher relative position of the tail and a narrower caudal peduncle are more efficient. The obtained results show the existence of a relationship between fish morphology and swimming performance, with potential consequences due to selection pressures associated with velocity barriers and their implications on behavioural and dispersal processes. To sum up, velocity barriers could exert a selection pressure on nase populations, so the fishway design and removal should be (re)considered.

The Influence of Individual Set-Pieces in Elite Rink Hockey Match Outcomes

The main objective of this study was to analyze the influence of individual set-pieces (Free Direct Hits and Penalties) in elite rink hockey match outcomes in different game situations. A sample of 161 matches played between high-standard teams during ten consecutive seasons (2009–2010 to 2018–2019) were analyzed using a binary logistic regression. The full evaluated model was composed of an explanatory variable (set-pieces scored) and five potential confounding and interaction variables (match location, match level, match importance, extra time, and balanced score). However, the final model only included one significant interaction variable (balanced score). The results showed that scoring more individual set-pieces than the opponent was associated with victory (OR = 6.1; 95% CI: 3.7, 10.0) and was more relevant in unbalanced matches (OR = 19.5; 95% CI: 8.6, 44.3) than in balanced matches (OR = 2.3; 95% CI: 1.2, 4.5). These findings indicate that individual set-pieces are strongly associated with match outcomes in matches played between high-standard teams. Therefore, it is important for teams to excel in this aspect, and it is suggested that these data can encourage coaches to reinforce the systematic practice of individual set-pieces in their training programs. Additionally, it is suggested that teams have specialist players in this kind of action to mainly participate in these specific match moments.

Global Diffusion of a New Product Class: A Study of New World Wine

<p>Understanding the drivers of product diffusion in global markets is a key determinant of success in marketing a product internationally. Factors such as national culture that are specific to a particular country market play a significant role in determining the speed of diffusion in that market. Examining the diffusion of New World wine provides an opportunity to test existing theories in the context of a new product class innovation as opposed to a new product innovation (as is the case in the existing literature). In addition, the study of diffusion of a new product class provides an opportunity to measure diffusion rates, within global markets, against a key explanatory variable in diffusion theory, 'prior experience of a previously introduced idea'. This is due to the fact that global markets have had previous experience with other wine product classes, before the advent of New World wine. This explanatory variable is an important part of classic diffusion theory however it has not been tested in a cross national sense. Through the study of 47 countries/regions that encompasses all countries in the world (at time of publication), the research found a strong negative correlation between 'prior experience of a previously introduced idea' and market share growth of New World wine across global markets. The author suggests that a key potential factor for this finding relates to the existence of a quality status hierarchy in existence in countries with higher levels of prior experience, resulting in a lower quality perception for a new product class in this market, when compared to a market that does not have an existing status hierarchy in place due to lower prior experience with a similar product. This concept potentially extends the cross national diffusion literature. In addition this research may provide important implications for managers in developing global marketing strategies.</p>

Global Diffusion of a New Product Class: A Study of New World Wine

<p>Understanding the drivers of product diffusion in global markets is a key determinant of success in marketing a product internationally. Factors such as national culture that are specific to a particular country market play a significant role in determining the speed of diffusion in that market. Examining the diffusion of New World wine provides an opportunity to test existing theories in the context of a new product class innovation as opposed to a new product innovation (as is the case in the existing literature). In addition, the study of diffusion of a new product class provides an opportunity to measure diffusion rates, within global markets, against a key explanatory variable in diffusion theory, 'prior experience of a previously introduced idea'. This is due to the fact that global markets have had previous experience with other wine product classes, before the advent of New World wine. This explanatory variable is an important part of classic diffusion theory however it has not been tested in a cross national sense. Through the study of 47 countries/regions that encompasses all countries in the world (at time of publication), the research found a strong negative correlation between 'prior experience of a previously introduced idea' and market share growth of New World wine across global markets. The author suggests that a key potential factor for this finding relates to the existence of a quality status hierarchy in existence in countries with higher levels of prior experience, resulting in a lower quality perception for a new product class in this market, when compared to a market that does not have an existing status hierarchy in place due to lower prior experience with a similar product. This concept potentially extends the cross national diffusion literature. In addition this research may provide important implications for managers in developing global marketing strategies.</p>

Electricity Market Liquidity and Price Spikes: Evidence from Hungary

This article examines how electricity market liquidity, renewable production and cross-border activity together in combination explain price spikes in the Hungarian Power Exchange day-ahead auctions. In the applied logit model, the dependent variable representing the price spike is binary, and the key explanatory variable is a modified bid-ask spread depicting liquidity. Weather-dependent renewable production and the difference between exports and imports appear as control variables in the model. The empirical analysis was based on data from 2017 and 2018. The results show that the control variables have no effect on the bid-ask spread and that the model explains 96 per cent of the spikes well, with an AUC-ROC of 0.75 and a Gini coefficient of 0.5. Based on the results, it may be worthwhile for traders to incorporate their data from sales and purchase curves into their forecasts, as this will improve their chances of successfully predicting extreme prices.

DETERMINANTS OF INFLATION IN INDIA IN A DYNAMIC SETUP

The present study empirically examines the factors accounting for inflation in India in an open economy framework by utilizing the bounds testing approach to cointegration for the 2006: Q3-2019: Q4 period. The findings reveal the existence of a long-run relationship with the household survey-based inflation expectation, real output, narrow money aggregate and interest rate as important determinants of inflation. The study concludes that inflation is well explained by a combination of structural and monetary factors. Notably, the significance of inflation expectation as an important explanatory variable corroborates the utilization of inflation forecast by the RBI as an intermediate target in the flexible inflation targeting framework. In this backdrop, it is imperative for RBI to conduct a high frequency inflation expectations survey of households to account for frequent information updation on the part of certain groups of households.

Transcutaneous and End-Tidal CO2 Measurements in Hypoxia and Hyperoxia

BACKGROUND: Transcutaneous measurement of carbon dioxide (CO2) has been proposed for physiological monitoring of tactical jet aircrew because in some clinical settings it mirrors arterial CO2 partial pressure (Paco2). End-tidal monitoring in laboratory settings is known to give high-fidelity estimates of Paco2.METHODS: The correspondence between end-tidal (PETco2) and transcutaneous Pco2 (tcPco2) was examined in healthy volunteers under laboratory conditions of hyperoxia and hypoxia. Rest and exercise, skin heating and cooling, hyperventilation, and induced CO2 retention were employed.RESULTS: Neither measure followed all known changes in Paco2 and tcPco2 changed when the skin temperature near the probe changed. Bland-Altman analysis showed significant nonzero slopes under most conditions. Regression analysis indicated that oxygen partial pressure (Po2) in tissue measured as transcutaneous Po2 (tcPo2) is an important explanatory variable for tcPco2 in addition to PETco2, and that local skin temperature also has an effect. Additionally, absorption atelectasis from breathing 100% O2 may cause PETco2 to deviate from Paco2.DISCUSSION: Even as a trend indicator for Paco2, tcPco2 is not useful under conditions that resemble those in the highly dynamic tactical jet aircraft environment. PETco2 is also not a good indicator of CO2 status in pilots who breathe nearly 100% O2.Shykoff BE, Lee LR, Gallo M, Griswold CA. Transcutaneous and end-tidal CO2 measurements in hypoxia and hyperoxia. Aerosp Med Hum Perform. 2021; 92(11):864-872.