CASP microdomain formation requires cross cell wall stabilization of domains and non-cell autonomous action of LOTR1

Efficient uptake of nutrients in both animal and plant cells requires tissue-spanning diffusion barriers separating inner tissues from the outer lumen/soil. However, we poorly understand how such contiguous three-dimensional superstructures are formed in plants. Here, we show that correct establishment of the plant Casparian Strip (CS) network relies on local neighbor communication. We show that positioning of Casparian Strip membrane domains (CSDs) is tightly coordinated between neighbors in wild-type and that restriction of domain formation involves the putative extracellular protease LOTR1. Impaired domain restriction in lotr1 leads to fully functional CSDs at ectopic positions, forming 'half strips'. LOTR1 action in the endodermis requires its expression in the stele. LOTR1 endodermal expression cannot complement, while cortex expression causes a dominant-negative phenotype. Our findings establish LOTR1 as a crucial player in CSD positioning acting in a directional, non-cell-autonomous manner to restrict and coordinate CS positioning.

A Shaky “Wedge”: An Analysis of India’s Strategic Behavior under the US “Indo-Pacific” Strategy

America’s Indo-Pacific strategy is essentially a combination of its Asia-Pacific and Indian strategies: through the consolidation of its strategic alliances, it can deepen its relations with Asia-Pacific allies, and through its “wedging strategy” it can pull India into its orbit to become a strategic “fulcrum” bridging the region. India was both a key member and leader of the non-aligned movement, while also previously forming a “quasi-alliance” with the Soviet Union. At present, it has responded to the US Indo-Pacific strategy with cautious initiative. From the vantage point of a state targeted vis-à-vis a wedging strategy, India’s strategic behavior is shaped by the strategic environment, its primary strategic objectives, and the relations between allies within the context of a unique strategic environment. This paper identifies two types of strategic environments: the general strategic environment and the specialized strategic environment; it further identifies primary strategic objectives as economic development, sovereign integrity and independence, national security and great state status (especially with respect to that of regional or global major powers); meanwhile, the alliance relationship is defined according to the extent to which there are divisions across state interests and the capacity of states to act autonomously vis-à-vis the alliance. Through an analysis of India’s diplomatic experiences, this paper argues that against the backdrop of America’s “Indo-Pacific” strategy and a lose general strategic environment, the primary strategic objective of achieving major state status and a “large divide over interests and a major space for autonomous action” shape India’s cautious initiative with respect to its alliance relationships.

From Unicorns to Mules: Phillip Pettit, Hannah Arendt, and the Liberal Human Rights Regime

<p>The research presented here is an effort to interpret the discrepancy between the theoretical inalienability of human rights and the ease with which they are alienated in practice; a paradox Hannah Arendt regarded as the most conspicuous and cruel contradiction of human rights discourse. Proponents of the contemporary human rights regime have recognised that two principal characteristics of liberal human rights politics—namely, the double appellation of the Rights of Man and Citizen and an insistence on sovereignty and power-politics—directly contribute to this paradox. Nonetheless, they deem the current approach to combating rights violations to be ‘the best we can hope for’. After discussing this pragmatic liberal approach, this paper continues by analysing the alternative approaches championed by two republican traditions which criticise liberal human rights—Pettit’s neo-republicanism and Arendt’s participatory republicanism. The former of these proposes an institutional commitment to the rights of the citizen, whereas the latter deems the direct action of political subjects to be the most effective form of guaranteeing written rights in practice. Finally, in agreement with Arendt’s thought, this paper argues that while liberal power-politics and neo-republican institutionalism have their place in human rights politics, rights are at their most secure as expressions of autonomous action.</p>

From Unicorns to Mules: Phillip Pettit, Hannah Arendt, and the Liberal Human Rights Regime

<p>The research presented here is an effort to interpret the discrepancy between the theoretical inalienability of human rights and the ease with which they are alienated in practice; a paradox Hannah Arendt regarded as the most conspicuous and cruel contradiction of human rights discourse. Proponents of the contemporary human rights regime have recognised that two principal characteristics of liberal human rights politics—namely, the double appellation of the Rights of Man and Citizen and an insistence on sovereignty and power-politics—directly contribute to this paradox. Nonetheless, they deem the current approach to combating rights violations to be ‘the best we can hope for’. After discussing this pragmatic liberal approach, this paper continues by analysing the alternative approaches championed by two republican traditions which criticise liberal human rights—Pettit’s neo-republicanism and Arendt’s participatory republicanism. The former of these proposes an institutional commitment to the rights of the citizen, whereas the latter deems the direct action of political subjects to be the most effective form of guaranteeing written rights in practice. Finally, in agreement with Arendt’s thought, this paper argues that while liberal power-politics and neo-republican institutionalism have their place in human rights politics, rights are at their most secure as expressions of autonomous action.</p>

A Real Entity Theory of Company Law

This chapter provides an overview of a real entity theory of company law. It begins by exploring three main theories of the company. The first theory explains the company as a contract; it forms the basis on which agency theory builds. The second theory conceives the company as a concession of the state, while the third theory characterizes the company as a real entity. The chapter then looks at a modern version of real entity theory and its application to company law. According to real entity theory, organizations or firms are social phenomenon outside of the law and they are autonomous actors in their own right. This occurs because human beings change their behaviour when they act as members of a group or an organization. Company law finds this phenomenon and evolves with a view to supporting autonomous action by organizations.

The Comparative Politics of Environmental Activism in Russia

How do environmental nongovernmental organizations (NGOs) continue their activism under conditions of increasingly authoritarian governance? In the 1990s, during the immediate post-Soviet period, Russian environmental organizations developed activist strategies in a period of political instability and economic recession. These strategies roughly corresponded to Hirschman’s ideal types of exit, voice, and loyalty. Since that time, many of the conditions that facilitated the creation of these organizational types have changed. Foreign funding for civil society development declined sharply, the government has embraced a more coercive approach to shaping social activism, and social media provide new platforms and resources for environmentalists. Following the passage of the 2012 Law on Foreign Agents, new forms of environmentalism in Russia emerged to adapt to and challenge the government’s effort to contract space for autonomous action. These activist strategies complicate Hirschman’s ideal types in ways that illuminate how environmentalism may endure under authoritarian regimes in Russia and beyond.

PROTECTIVE ROLE FOR SMOOTH MUSCLE CELL HEPCIDIN IN ABDOMINAL AORTIC ANEURYSM

Rationale: Hepcidin (HAMP) is a hormone produced primarily in the liver. It controls systemic iron homeostasis by inhibiting the iron exporter ferroportin (FPN) in the gut and spleen, respective sites of iron absorption and recycling. HAMP and FPN are also found ectopically in tissues not involved in systemic iron homeostasis. The physiological functions of ectopic HAMP and FPN are only just beginning to be uncovered. We observed that HAMP expression is markedly increased in smooth muscle cells (SMCs) of abdominal aortic aneurysms (AAA), both in patients and in an experimental mouse model of AAA. Objective: To understand the role of SMC-derived HAMP in the pathophysiology of AAA. Methods and Results: We generated mice harbouring an inducible, SMC-specific deletion of the hamp gene. We then applied the experimental model of AAA and simultaneously induced deletion of hamp in SMCs. We found that these mice developed large aneurysms and had greater incidences of rupture and of fatal dissection than mice with intact hamp in SMCs. A similar phenotype was observed in mice harbouring an inducible SMC-specific knock-in of HAMP-resistant FPNC326Y. Additionally, we observed that expression of Lipocalin-2 (LCN2), a protein known to promote AAA, was suppressed in AAA tissue from patients and from mice with intact hamp in SMCs, but not in mice lacking hamp in SMCs. Treatment of these mice with a LCN2-neutralising antibody protected them from the otherwise detrimental effects of loss of hamp in SMCs. Conclusions: The present study demonstrates that the rise in SMC-derived HAMP within the aneurysm tissue is protective in the setting of AAA, and that such protection involves the cell-autonomous action of HAMP, and suppression of local LCN2. These findings are the first example of a protective role for ectopic HAMP in disease. They expand understanding of the multifaceted functions of HAMP outside the liver.

Achieving Ethical Algorithmic Behaviour in the Internet of Things: A Review

The Internet of Things is emerging as a vast, inter-connected space of devices and things surrounding people, many of which are increasingly capable of autonomous action, from automatically sending data to cloud servers for analysis, changing the behaviour of smart objects, to changing the physical environment. A wide range of ethical concerns has arisen in their usage and development in recent years. Such concerns are exacerbated by the increasing autonomy given to connected things. This paper reviews, via examples, the landscape of ethical issues, and some recent approaches to address these issues concerning connected things behaving autonomously as part of the Internet of Things. We consider ethical issues in relation to device operations and accompanying algorithms. Examples of concerns include unsecured consumer devices, data collection with health-related Internet of Things, hackable vehicles, behaviour of autonomous vehicles in dilemma situations, accountability with Internet of Things systems, algorithmic bias, uncontrolled cooperation among things, and automation affecting user choice and control. Current ideas towards addressing a range of ethical concerns are reviewed and compared, including programming ethical behaviour, white-box algorithms, black-box validation, algorithmic social contracts, enveloping IoT systems, and guidelines and code of ethics for IoT developers; a suggestion from the analysis is that a multi-pronged approach could be useful based on the context of operation and deployment.

Research of relationship of functional-role positions (ego-states) with indicators of future specialists’ independence

The purpose of empirical research is to establish the relationship of functional-role positions (ego-states) with other indicators of future professionals ’independence to establish the level of manifestation of functional ego-states. The investigation contemplated methods are projective methods, psychodiagnostic methods with valid scales, author’s method “Indirect self-assessment of independence” (“ISI”) and the author’s questionnaire “Persuasion in independence” (“PII”). The results indicated that the most important for the respondents the value of independence, the more they are focused on taking into account the actual possibilities and autonomous action on the basis of objective data (ego-state “Adult”), as well as control, management, criticism of other people (ego-state “Parent-controller”). Therefore, the more autonomous respondents, the less interdependent they are, and vice versa.

Europeanised policy making in Ireland

In this chapter the influence of the European Union (EU) on the policy making process and policy analysis in Ireland is examined. The country’s EU membership has been marked by periods of volatility, with support for the institution wavering at times. Membership has aided Ireland’s global positioning and ability to cope with international competition, but has also constrained the policy analysis process as autonomous action by national actors can be limited by legal commitments to EU membership. Also, policy initiatives emanating from the EU have sometimes challenged conservative domestic constituencies. The chapter provides a pair of case studies to demonstrate a variety of Europeanisation effects across the Irish policy analysis spectrum. Being part of the EU has produced distinct patterns of adaptation and contestation at the domestic level. The consequences of EU membership have been both expansive and comprehensive, impacting on a myriad of domestic issues, sectors and policies.