scholarly journals The Maturity of Female Gonads of G2 Transgenic Mutiara Catfish (Clarias sp.)

2021 ◽  
pp. 9-19
Author(s):  
Ayuniar Puteri ◽  
Ibnu Dwi Buwono ◽  
Roffi Grandiosa Herman ◽  
. Iskandar
Keyword(s):  
Reproductive Performance ◽  
Clarias Gariepinus ◽  
Transgenic Fish ◽  
Egg Weight ◽  
Gonad Maturity ◽  
Gonadal Maturity ◽  
Randomized Design ◽  
Gonad Growth ◽  
Completely Randomized Design ◽  
Egg Diameter

The G2 trangsenic Mutiara catfish (Clarias sp.) (MTG) is a Mutiara catfish that is inserted with the CgGH gene (Clarias gariepinus Growth Hormone) through the transgenesis. The effect of transgenesis stimulates gonad growth of G2 transgenic Mutiara catfish (Clarias sp.) faster than non-transgenic fish. Study aimed to analyze the maturity of the gonads and the spawning ability of female G2 transgenic Mutiara catfish (Clarias sp.) to obtain superior broodstock candidates. Experimental method with completely randomized design (pair of parents used as treatment and repeated four times) for spawning was used for this study. Three pairs of parent G2 were crossed semi-artificially as treatment A (female 1 MTG G2 crossed with male 1 MTG G2), B (female 2 MTG G2 crossed with male 2 MTG G2) and C (female 3 MTG G2 crossed with male 3 MTG G2). The results showed that the performance of female G2 transgenic Mutiara catfish (Clarias sp.) (treatment A, B and C) was higher given non-transgenic with an average relative fecundity of 82,438 eggs / kg of broodstock, an average egg diameter of 1.76 mm and an average egg weight 1.75 mg. These indications suggest that GH transgenesis increases gonadal maturity. The gonad maturity profile reached the stage of complete maturity (full ripe) compared to Sangkuriang catfish (Clarias gariepinus) (immature gonads). Therefore it is necessary to compare the reproductive performance of G2 transgenic Mutiara catfish (Clarias sp.) with non-transgenic fish (Sangkuriang) as candidates for superior broodstock of catfish.

scholarly journals THE STIMULATION OF GONAD MATURITY OF ASIAN REDTAIL CATFISH Hemibagrus nemurus (Valenciennes, 1840) THROUGH INDUCTION OF OOCYTE DEVELOPER (Oodev) HORMONE

2019 ◽  
Vol 8 (1) ◽  
pp. 965
Author(s):  
Devika Kharisma Putri ◽  
Tarsim Tarsim ◽  
Deny Sapto Chondro Utomo ◽  
Indra Gumay Yudha
Keyword(s):  
Research Method ◽  
Spawning Season ◽  
Gonad Maturity ◽  
Gonadal Maturity ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Egg Diameter ◽  
Somatic Index ◽  
Stimulation Of

Oodev hormone application has been applied to stimulate gonadal maturity so that fish fry could be available outside the spawning season. This research aimed to study the effect of Oodev hormone on the gonadal maturity of asian redtail catfish Hemibagrus nemurus (Valenciennes, 1840). This research method used a completely randomized design (CRD) with 4 doses treatments, i.e 0, 0,25, 0,50, 1,00 ml/kg. A total of 8 fish were used as replications for each treatment. The parameters measured were the gonado somatic index (GSI) and egg diameter. The results showed that the effect of Oodev hormone on GSI and egg diameter was significantly different (P<0.05). A higher Oodev hormone dose caused an increase of GSI and egg diameter size. The recommended Oodev dose for application is 1 ml/kg.

scholarly journals MATURASI LELE MUTIARA Clarias gariepinus BETINA MELALUI PENAMBAHAN HORMON OODEV DAN ESTRADIOL-17ß PADA PAKAN

2021 ◽  
Vol 9 (1) ◽  
pp. 30-40
Author(s):  
Oktavia Rugus Ayu Enditha ◽  
Deny Sapto Chondro Utomo ◽  
Munti Sarida
Keyword(s):  
Key Words ◽  
Reproductive Cycle ◽  
Clarias Gariepinus ◽  
Spawning Season ◽  
Maturity Index ◽  
African Catfish ◽  
North African ◽  
Gonadal Maturity ◽  
Randomized Design ◽  
Completely Randomized Design

The problem in Mutiara strain of North African catfish Clarias gariepinus culture is that the production of both larvae and seeds is still inhibited by the seasonal reproductive cycle where the catfish only spawn once during the spawning season.  The purposes of this study were to evaluate the effect of administration of oodev and estradiol-17ß on the maturity process and to determine the best doses in accelerating the maturion of female Mutiara strain. This study used a Completely Randomized Design with 5 treatments and 3 individual replications. The treatments were combination between dose Oodev (PMSG and Anti-Dopamine) and dose Estradiol-17ß in feed (both doses in ml/kg feed):A(0+0), B(0.5+0), C(0+0.6), D(0.5+0,6), E(1+1). The results of this study showed that the administration of Oodev and Estradiol-17ß significant affect on gonadal maturity index about 82.21% compared to control and acclerating maturation of female mutiara strain with doses the best is the treatment E (oodev 1 ml/kg feed+estradiol-17ß 1 ml/kg feed).Key words: Clarias gariepinus, dose, estradiol-17ß, maturation, oodev

scholarly journals Effects of GnRH and Anti-Dopamine on Gonad Maturation of Osteochilus melanopleurus (Bleeker, 1852)

2021 ◽  
Vol 934 (1) ◽  
pp. 012014
Author(s):  
N Asiah ◽  
N Aryani ◽  
N Nuraini ◽  
I Lukistyowaty ◽  
Y Harjoyudanto
Keyword(s):  
Body Weight ◽  
Research Method ◽  
Economic Value ◽  
Gonad Maturation ◽  
Gonad Maturity ◽  
River Waters ◽  
Randomized Design ◽  
Injection Dose ◽  
Completely Randomized Design ◽  
Egg Diameter

Abstract The Osteochilus melanopleurus (Bleeker, 1852) is one of the endogenous fish in the Siak River waters of Riau province which has high economic value. However, the fulfillment of needs still depends on the catch in nature. Efforts to overcome these problems are the availability of mature gonads. Broadly to accelerate the maturation of gonads through hormone induction until the fish are ready to be spawned to produce fish fry continuously. The purpose of this study was to analyze the effect of gonadotropin and anti-dopamine hormone induction with the trademark “Oodev” on the final gonad maturity (TKG) of the prospective broodstock of O. melanopleurus. The research was conducted from April to August 2021 in the fish rearing column and the experimental pond, Faculty of Fisheries and Marine Science. The research method was a Completely Randomized Design with 4 treatment levels and 3 replications, while the treatments were P0 (without Oodev injection), P1 (Oodev dose 0.5 mL/kg), P2 (0.7 mL/kg), and P3 (0,9 mL/kg). Measured responses: percentage of broodstock that reached TKG and egg diameter. The results of the research that have been carried out, the measurement of the diameter of the eggs produced showed that the use of the Oodev hormone at a dose of 0.7 mL/kg body weight of O.melanopleurus given every week for twelve weeks was able to stimulate the development of the gonads of O.melanopleurus. Ovaprim injection of 0.7 mL/kg body weight produced 89,566 eggs with a latency of 4 hours. oodev injection dose of 0.7 ml/kg BW or P2 treatment has succeeded in achieving a TKG of 82%.

Pengaruh ovaprim, aromatase inhibitor, dan hipofisa terhadap kualitas telur ikan Lele Dumbo (Clarias gariepinus)

2017 ◽  
Vol 5 (1) ◽  
Author(s):  
Erwin A. Aziz ◽  
Ockstan Kalesaran
Keyword(s):  
Survival Rate ◽  
Experimental Design ◽  
Aromatase Inhibitor ◽  
Fish Larvae ◽  
Clarias Gariepinus ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
The Difference ◽  
Hatching Eggs

This study aimed to determine the effect of ovaprim hormone, aromatase inhibitor and pituitary on the quality of the catfish eggs (Clarias gariepinus). Experimental Design used was Completely Randomized Design (CRD) with four treatments, each with three replications. Treatment A: ovaprim; treatment B: Aromatase inhibitors, treatment C: hypophysis and treatment D: Control. The results showed that the difference in treatment gave highly significant effect on fertilization and hatching eggs but no significant effect on the survival rate of larvae. Aromatase inhibitor hormone was the best because it provided highly significant effect on fertilization (92.66%), hatchability of eggs (95%), and surviva rate (81.33%) of fish larvae.   Keywords : Clarias gariepinus. Ovaprim, Aromatase Inhibitor, Hypophysis, egg, larvae

PSIII-9 The Effect of Sire Line on Reproductive Performance During Lactation

2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 169-169
Author(s):  
H Manu ◽  
M Fletcher ◽  
K Fang ◽  
Sudario Roberto Silva Junior ◽  
J Dunkelberger ◽  
...  
Keyword(s):  
Reproductive Performance ◽  
Large White ◽  
Randomized Design ◽  
Genetic Source ◽  
Completely Randomized Design ◽  
Line P ◽  
Synthetic Line

Abstract The objective of this study was to estimate the effect of sire line on sow and piglet performance during lactation. Commercial Landrace x Large White females (n = 60) were bred to one of the following sire lines: a purebred Duroc line (Line S1), a synthetic (Line S2), or purebred Duroc (Line S3) from a different genetic source. Matings were balanced by line and parity. Females were fed a common gestation and lactation diet during these respective phases. Sow and piglet data were analyzed as a completely randomized design using the GLM procedure of SAS. Results show that, during lactation, females bred to line S3 lost significantly (P = 0.01) more BW (-16.93 ± 3.56 kg) than females bred to line S2 (-2.00 ± 3.50 kg). However, no evidence of a difference (P = 0.18) in BW loss was detected between females bred to line S3 (-16.93 ± 3.56 kg) vs. Line S1 (-7.90 ± 3.56 kg). Further, sows bred to line S3 weaned significantly (P = 0.03) more piglets (12.0 ± 0.28) than females bred to line S2 (10.9 ± 0.27), but not (P = 0.1) compared to females bred to line S1 (11.7 ± 0.27). No significant effect of line (P &gt; 0.05) or parity (P &gt; 0.05) on total number of piglet’s born, number born alive, number of stillborn piglets, or preweaning mortality was detected. The number of mummified fetus tended to be lower in line S2 (0.17 vs 0.66; P = 0.079) and line S3 (0.12 vs 0.66; P = 0.051) sows compared with line S1 sows, respectively. In conclusion, sire line did not have a significant effect on total number born, number born alive, number stillborn, or preweaning mortality. However, females bred to line S3 lost significantly more BW during lact but weaned significantly more piglets than females bred to line S2.

scholarly journals Fish by-product meal in diets for commercial laying hens

2017 ◽  
Vol 39 (3) ◽  
pp. 273
Author(s):  
André Ferreira Silva ◽  
Frank George Guimarães Cruz ◽  
João Paulo Ferreira Rufino ◽  
Waldo Mateus Plácido Miller ◽  
Nathália Siqueira Flor ◽  
...  
Keyword(s):  
Production Cost ◽  
Egg Production ◽  
Laying Hens ◽  
Polynomial Regression ◽  
Egg Quality ◽  
Feed Conversion ◽  
Egg Weight ◽  
Feed Cost ◽  
Randomized Design ◽  
Completely Randomized Design

 This study aimed to evaluate the increasing levels (0, 1, 2, 3 e 4%) of fish by-product meal in diets for laying hens on performance, egg quality and economic analysis. A total of 160 Dekalb White hens with 52-wk old were distributed in a completely randomized design with five treatments and four replicates of eight birds each. The experiment lasted 84 days divided into four periods of 21 days. Estimates of fish by-product meal levels were determined by polynomial regression. Differences (p < 0.05) were detected for all variables of performance, in egg weight, yolk and albumen percentage, yolk and albumen height, feed cost and production cost, in which the inclusion of fish by-product meal in the diets showed better results. It can be concluded that fish by-product meal can be used in diets for hens as alternative feed, with better results in egg production, feed conversion, egg weight, yolk-albumen ratio and a reduction in feed cost and production cost. 

scholarly journals The evaluation of turmeric (Curcuma longa) supplementation within feed as an antioxidant towards growth performance of catfish Clarias gariepinus Burchell 1822 in zero water exchange condition

2020 ◽  
Vol 20 (2) ◽  
pp. 105
Author(s):  
Thoy Batun Citra Rahmadani ◽  
Dedi Jusadi ◽  
Mia Setiawati ◽  
Yuni Puji Hastuti
Keyword(s):  
Growth Performance ◽  
Liver Damage ◽  
Water Exchange ◽  
Curcuma Longa ◽  
Clarias Gariepinus ◽  
African Catfish ◽  
Randomized Design ◽  
Growth Performances ◽  
Exchange Condition ◽  
Completely Randomized Design

The objective of this research was to evaluate the supplementation of turmeric in the diet on antioxidant status and growth performance of African catfish (Clarias gariepinus) in zero water exchange condition. This study used a completely randomized design with 4 treatments and 4 replications. Each treatment consisted of feed supplementation turmeric at dosage of 0; 2.5; 5 or 7.5 g kg-1 diet. One hundred catfish juvenile (5.95±0.05 g) were stocked in intermediate bulk container (IBC) tank (1×1×1 m3) and rearing in zero water exchange condition for 60 days. Catfish were fed at satiation twice a day, in the morning and evening. The results showed that an increase in antioxidant content in catfish fed with the addition of turmeric, which simultaneously also reduced the percentage of liver damage. The parameters of liver damage can be seen from several parameters i.e. pale liver, droplet fat and fat content in the addition of turmeric treatment is lower than without the addition of turmeric. However, catfish fed with the addition of turmeric did not show significant results in terms of growth performances.

scholarly journals EFEK PENAMBAHAN ENZIM (Amylase, Protease, Xylanase) DALAM PAKAN TERHADAP BERAT TELUR DAN DIAMETER KUNING TELUR PADA ITIK CAMPURAN

2021 ◽  
Vol 11 (1) ◽  
pp. 39-47
Author(s):  
Bagus Uda Palgunadi ◽  
Ratna Widyawati ◽  
Dian Ayu Kartika Sari ◽  
Halimah Tusadiah
Keyword(s):  
Egg Yolk ◽  
Egg Weight ◽  
Experimental Animals ◽  
Anova Test ◽  
Average Value ◽  
Randomized Design ◽  
Significant Difference ◽  
Completely Randomized Design

The purpose of this research aims to determine the effect of adding enzymes (amylase, protease, xylanase) in feed on egg weight and egg yolk diameter in mixed ducks. The experimental animals used were 24 laying ducks. The design used was a completely randomized design (CRD) with 4 treatments and 6 replications. The four treatments were P0 (Control), P1 (6 ducks with Avizym1502®5gram / kg), P2 (6 ducks with Avizym1502® 10gram / kg), and P3 (6 ducks given Avizym1502). ® 15gram / kg). This treatment is carried out once a day for 9 weeks. Before the treatment, the ducks were adapted for a week. After that, the initial egg weight and yolk diameter were measured at week 0, week 6, and week 9. Data obtained from the results of weighing eggs found an average value of P0 (59.1667 ± 3.9200a) g, P1 5gram / kg (56.1667 ± 2.9268a) g, P2 10gram / kg (60.1667 ± 4.9966a) g, and P3 15gram / kg (67.6667 ± 2.7325ᵇ) g. The results of data from examining the diameter of the egg yolk found an average value of P0 (45.03 ± 1.2956a) mm, P1 5gram / kg (44.35 ± 0.7969a) mm, P2 10gram / kg (45.75 ± 1.5694a) mm, and P3 15gram / kg (48.83 ± 0.7394ᵇ) mm. The data obtained were then analyzed using the ANOVA test which statistically showed that there was a significant difference (P <0.05) between egg weight and yolk diameter in mixed ducks treated with enzymes (amylase, protease, xylanase) in the feed.

scholarly journals 173 The effects of Fescue EMTTM Mineral Defense on stocker heifer growth while grazing tall fescue

2019 ◽  
Vol 97 (Supplement_1) ◽  
pp. 60-60
Author(s):  
Anna R Taylor ◽  
Randy Dew ◽  
Ken Bryan ◽  
J Nathan Pike ◽  
T Ryan Lock
Keyword(s):  
Weight Gain ◽  
Reproductive Performance ◽  
Tall Fescue ◽  
Repeated Measures ◽  
Animal Nutrition ◽  
Forage Availability ◽  
Randomized Design ◽  
Endophyte Infection ◽  
Completely Randomized Design ◽  
Over Time

Abstract Previous research demonstrates grazing tall fescue can decrease reproductive performance and weight gain in cattle. The objective of this study was to evaluate Fescue EMTTM Mineral Defense (Cargill Animal Nutrition, Minneapolis, MN) on summer weight gain in cattle grazing tall fescue pastures in SW Missouri. Heifers (n = 120; initial BW = 236 ± 2.5 kg) were stratified by weight to replicated tall fescue pastures to either a control mineral treatment or Fescue EMT™ Mineral Defense treatment. Forage availability was estimated weekly by ultrasonic sensor. Pasture samples were collected every 21 d and analyzed for ergovaline concentrations. Heifer weights and blood prolactin were measured throughout the trial. Average daily mineral consumption was calculated by mineral offered less residual. Data were analyzed on a pen-mean basis as a completely randomized design using JMP with 6 pens/ treatment and 10 heifers/pen. Prolactin was analyzed as Repeated Measures in JMP. Initial weights between treatments were not different (P > 0.05). Endophyte infection measured 75% or greater in all pastures. No differences were detected in pasture ergovaline (149 ± 19 µg/kg) or pasture availability (2,600 ± 150 kg/ha) between treatments (P > 0.20 at each sampling). Heifer ADG consuming Fescue EMT™ Mineral Defense compared to control mineral was greater at 0.28 kg versus 0.22 kg resulting in total gains of 21.8 kg versus 16.6 kg, respectively (P < 0.05). However, blood prolactin numerically decreased over time in both treatments. Results from this trial demonstrate a 31% improvement in weight gain for cattle consuming Fescue EMTTM Mineral Defense compared with cattle consuming a control mineral while grazing toxic tall fescue.

scholarly journals Characteristics of Quality Semen, Hatching Arabic Chicken Eggs and Growth of Chicks from Crosses

2018 ◽  
Vol 3 (2) ◽  
pp. 40-47
Author(s):  
Rin Orin Ningrum ◽  
Takdir Saili ◽  
La Ode Baa
Keyword(s):  
Initial Phase ◽  
Semen Quality ◽  
Research Process ◽  
Feed Consumption ◽  
Feed Conversion ◽  
Egg Weight ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Chicken Eggs

This study aims to evaluate and analyze the characteristics of the quality of semen, and hatching of Arab chicken eggs and the growth of crosses from chicks. This research was conducted at the Permata Farmer Group business, Wua-wua Village, Wua-wua District Kendari, Southeast Sulawesi for three months. This study uses a Completely Randomized Design which is three treatments and six replications. The parameters measured were semen quality, egg weight, egg index, fertility, DOC weight gain, feed consumption, and feed conversion. The data analysis used is Analysis of Variance. The results of this study indicate that the consequences of crossing three males did not have a significant effect (P> 0.05) on the measured parameters. It can be seen in the research process which gives results that are not much different from the results of the crossing of three different males using the Arabic parent in the initial phase of production.

Sign in / Sign up

Export Citation Format

Share Document