scholarly journals Characterization of Soluble Sugar Content, Related Enzyme Activity and Gene Expression in the Fruits of ‘Minihyang’ Mandarin on Different Rootstocks

Horticulturae ◽  
2022 ◽  
Vol 8 (1) ◽  
pp. 47
Author(s):  
Ha Rim Hong ◽  
Eun Ui Oh ◽  
Seung Gab Han ◽  
Su Hyun Yun ◽  
Ho Bang Kim ◽  
...  
Keyword(s):  
Gene Expression ◽  
Enzyme Activity ◽  
Sugar Content ◽  
Soluble Sugar ◽  
Regulation Of Gene Expression ◽  
Fruit Size ◽  
Pcr Analysis ◽  
Total Sugar Content ◽  
Significant Difference ◽  
Days After Anthesis

‘Minihyang’ mandarin bears fruits with small size and high sugar content. ‘Minihyang’ mandarin grafted on trifoliate orange (TO) tends to be vigorous and develops water sprout open. It is associated with insufficient floral differentiation and fruit set. Recently, the use of Flying Dragon (FD) as rootstock with a high dwarf effect has been proposed to improve this situation. Therefore, this study was conducted to evaluate the effect of two different rootstock genotypes on tree growth, fruit yield, and fruit quality at the physiological, biochemical, and molecular levels. As a result of the study, in FD, tree vigor was stably maintained, fruit size was large, and the sugar content was high compared to the TO. Fructose, glucose, and sucrose of fruit continued to increase from development to maturity. In particular, fructose and sucrose were significantly higher in the fruits of the FD than those in TO at 150 and 220 days after anthesis. The total sugar content was also significantly higher in the fruit of the FD. The activities of SPS and SS associated with sucrose synthesis tended to be increased during the fruit maturity season, but there was no significant difference between the two rootstocks. On the other hand, the activities of SS and AI breaking down sucrose were high in FD at 150 and 220 days after anthesis. These results suggest that the unloading of sucrose might be increased and affect the sugar content. However, the results of real-time PCR analysis of gene expression related to sucrose metabolism did not show an association with changes in enzyme activity affecting sugar content. Therefore, further detailed studies on the process after the regulation of gene expression are likely to be needed.

Germination vigour difference of superior and inferior rice grains revealed by physiological and gene expression studies

2018 ◽  
Vol 156 (3) ◽  
pp. 350-357
Author(s):  
Y. F. Zhao ◽  
H. Z. Sun ◽  
H. L. Wen ◽  
Y. X. Du ◽  
J. Zhang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Sugar Content ◽  
Soluble Sugar ◽  
Yield Potential ◽  
Rice Grains ◽  
Number Of Factors ◽  
Significant Difference ◽  
Early Germination ◽  
Relative Water ◽  
Gene Expression Studies

AbstractSuperior and inferior rice grains have different weights and are located on the upper primary branch and lower secondary branches of the panicle, respectively. To study differences in germination vigour of these two types of grain, a number of factors were investigated from 0 to 48 h of germination. The present study demonstrated that in inferior grains the starch granule structure was looser at 0 h, with full water absorption at 48 h, while in superior grains the structure was tight and dense. Relative water content increased, and dry matter decreased, more rapidly in inferior grains than in superior ones. Abscisic acid and gibberellin levels, as well as α-amylase activity, also changed more rapidly in inferior grains, while soluble sugar content and amylase coding gene expression increased more rapidly in inferior than superior grains during early germination. The expression of OsGAMYB was higher in inferior grains at 24 h but higher in superior grains at 48 h. The phenotypic index of seedlings was higher in seedlings from superior grains at the two-leaf stage. However, the thousand-grain weight and yield per plant in superior and inferior plants showed no significant difference at harvest. The present study indicates that inferior grains germinate faster than superior ones in the early germination stage. Although inferior grains produced weaker seedlings, it is worthwhile using them in rice production due to their comparative yield potential over that of superior grains.

scholarly journals PHYSICO-CHEMICAL CHARACTERIZATION OF COCONUT ECOTYPES IN BENIN COASTAL ZONE

2021 ◽  
Vol 9 (8) ◽  
pp. 215-225
Author(s):  
Ulysse Ayihaou Daa-Kpode ◽  
Patrice Hodonou Avogbe ◽  
Gustave Djedatin ◽  
Daniel Datchiglo ◽  
Aristide Bakpe ◽  
...  
Keyword(s):  
Coastal Zone ◽  
Nutritional Value ◽  
Ph Value ◽  
Sugar Content ◽  
Soluble Sugar ◽  
Chemical Characterization ◽  
Titratable Acidity ◽  
Total Sugar Content ◽  
Physico Chemical

Coconut water is highly consumed for refreshment due to its nutritional value. However, its nutritional value varies according to the ecotype and stage of maturity of ecotype. In order to identify best ecotypes with high nutritious value, a physico-chemical composition of ten (10) ecotypes was performed at earlier stage of maturity (3 months). The measured parameters were: coconut height, mass of water, total sugar content, soluble sugar content, protein, titratable acidity and pH.  The obtained results showed that all parameters vary from one ecotype to another. All coconut ecotypes contain water slightly acid with a pH value less than 7.  Furthermore, there is no relationship between physical and biochemical parameters. However, within characterized ecotypes, Ecotype_2, appeared to have the best biochemical composition with a significant amount of water. This study provided important informations related to physico-chemical characteristics of coconuts ecotypes found in the coastal zone.

scholarly journals Analysis of pomological traits in new promising sweet cherry genotypes

Genetika ◽  
2013 ◽  
Vol 45 (3) ◽  
pp. 873-880 ◽  
Author(s):  
Milica Fotiric-Aksic ◽  
Tihomir Nikolic
Keyword(s):  
Sweet Cherry ◽  
Sugar Content ◽  
Fruit Size ◽  
Fruit Weight ◽  
Solid Content ◽  
Soluble Solid Content ◽  
Fresh Market ◽  
Total Sugar Content ◽  
Ripening Time ◽  
Chemical Content

Sweet cherry breeding programs are interested in developing new cultivars that are very early or very late in ripening, high yielding, with excellent fruit size and quality. The aim of this study was to evaluate new, promising, sweet cherry genotypes (G-1 and G-2) for ripening time, fruit morphology and fruit chemical content, and compare it with the standard sweet cherry cultivars (Bigarreau Jaboulay, B. H. Burlat, Seneca, Hedelfiger Riesenkirsche, Germersdorfer Grosse Kirsche, Emperor Francis and Bing). Experiment was done during four consecutive years (2006-2009) in Grocka, near Belgrade. The earliest fruit ripening was recorded in G-2 (22nd April) while the latest in `Bing` (2nd June). Fruit weight varied from 5.83 g (G-2) up to 8.93 g (G-1). Soluble solid content and total sugar content were the lowest in genotype G-2 (10.48%; 8.45%, respectively) but the highest in Emperor Francis (18.28%; 15.98%, respectively). No matter the fact that some standard cultivars showed better results for some traits, genotypes G-1 and G-2 are considered very promising. So, genotype G-1 could be intended for table consumption since it ripens 2-3 days after cv. B. H. Burlat, but have much higher fruit weight. Also, genotype G-2, the earliest in this trial, showed satisfactory fruit weight for this ripening time, which makes it very interesting for fresh market production.

scholarly journals Spatial regulation of gene expression in nonsyndromic sagittal craniosynostosis

2018 ◽  
Vol 22 (6) ◽  
pp. 620-626 ◽  
Author(s):  
Garrett N. Cyprus ◽  
Jefferson W. Overlin ◽  
Rafael A. Vega ◽  
Ann M. Ritter ◽  
René Olivares-Navarrete
Keyword(s):  
Gene Expression ◽  
Wnt Signaling ◽  
Transforming Growth Factor ◽  
Quantitative Polymerase Chain Reaction ◽  
Regulation Of Gene Expression ◽  
Micro Ct ◽  
Sagittal Suture ◽  
Sagittal Craniosynostosis ◽  
Significant Difference ◽  
Suture Fusion

OBJECTIVECranial suture patterning and development are highly regulated processes that are not entirely understood. While studies have investigated the differential gene expression for different sutures, little is known about gene expression changes during suture fusion. The aim of this study was to examine gene expression in patent, fusing, and fused regions along sagittal suture specimens in nonsyndromic craniosynostosis patients.METHODSSagittal sutures were collected from 7 patients (average age 4.5 months) who underwent minimally invasive craniotomies at the Children’s Hospital of Richmond at VCU under IRB approval. The sutures were analyzed using micro-CT to evaluate patency. The areas were classified as open, fusing, or fused and were harvested, and mRNA was isolated. Gene expression for bone-related proteins, osteogenic and angiogenic factors, transforming growth factor–β (TGF-β) superfamily, and Wnt signaling was analyzed using quantitative polymerase chain reaction and compared with normal sutures collected from fetal demise tissue (control).RESULTSMicro-CT demonstrated that there are variable areas of closure along the length of the sagittal suture. When comparing control samples to surgical samples, there was a significant difference in genes for Wnt signaling, TGF-β, angiogenic and osteogenic factors, bone remodeling, and nuclear rigidity in mRNA isolated from the fusing and fused areas of the sagittal suture compared with patent areas (p < 0.05).CONCLUSIONSIn nonsyndromic sagittal craniosynostosis, the affected suture has variable areas of being open, fusing, and fused. These specific areas have different mRNA expression. The results suggest that BMP-2, FGFR3, and several other signaling pathways play a significant role in the regulation of suture fusion as well as in the maintenance of patency in the normal suture.

scholarly journals Regulation of proton-translocating V-ATPases.

1997 ◽  
Vol 200 (2) ◽  
pp. 225-235 ◽  
Author(s):  
H Merzendorfer ◽  
R Gräf ◽  
M Huss ◽  
W R Harvey ◽  
H Wieczorek
Keyword(s):  
Gene Expression ◽  
Enzyme Activity ◽  
Plasma Membranes ◽  
Regulation Of Gene Expression ◽  
Transcriptional Level ◽  
Structural Domains ◽  
Sh Groups ◽  
Signalling Molecules ◽  
Membrane Spanning ◽  
Regulation Of Enzyme Activity

Vacuolar-type ATPases (V-ATPases) are proton-translocating enzymes that occur in the endomembranes of all eukaryotes and in the plasma membranes of many eukaryotes. They are multisubunit, heteromeric proteins composed of two structural domains, a peripheral, catalytic V1 domain and a membrane-spanning V0 domain. Both the multitude of locations and the heteromultimeric structure make it likely that the expression and the activity of V-ATPases are regulated in various ways. Regulation of gene expression encompasses control of transcription as well as control at the post-transcriptional level. Regulation of enzyme activity encompasses many diverse mechanisms such as disassembly/reassembly of V1 and V0 domains, oxidation of SH groups, control by activator and inhibitor proteins or by small signalling molecules, and sorting of the holoenzyme or its subunits to target membranes.

Effect of shading during growth on the soluble sugar contents of short rotation ryegrass.

1961 ◽  
Vol 1 (3) ◽  
pp. 153 ◽  
Author(s):  
JF Melvin ◽  
MA Sutherland
Keyword(s):  
Sugar Content ◽  
Soluble Sugar ◽  
Total Sugar ◽  
Total Sugar Content ◽  
Short Rotation

The effect of shading for 64 hours before cutting on the soluble sugar contents of short rotation ryegrass has been determined at weekly intervals during growth. From grass with five to six leaves emergent to the stage of growth usually accepted for hay-making, the average reduction in the total sugar content of the shaded plants was about 30 per cent, made up of decreases in sucrose (12 per cent), oligosaccharides (5 per cent) and fructosans (13 per cent).

221 EPIGENETIC DYNAMICS OF PLURIPOTENCY GENES IN THE CONTEXT OF DIFFERENTIATION AND NUCLEAR REPROGRAMMING DETERMINED BY Q2ChIP, A QUICK AND QUANTITATIVE CHROMATIN IMMUNOPRECIPITATION TECHNIQUE APPLICABLE TO SMALL CELL SAMPLES

2007 ◽  
Vol 19 (1) ◽  
pp. 227 ◽  
Author(s):  
J. A. Dahl ◽  
C. K. Taranger ◽  
P. Collas
Keyword(s):  
Gene Expression ◽  
Chromatin Immunoprecipitation ◽  
Regulation Of Gene Expression ◽  
Cell Extract ◽  
Proximal Promoter ◽  
Pcr Analysis ◽  
Cross Linking ◽  
Nuclear Reprogramming ◽  
Distal Enhancer ◽  
Developmentally Regulated

Interactions between proteins and DNA are essential for cellular functions such as genomic stability, DNA replication and repair, chromosome segregation, transcription, and epigenetic silencing of gene expression. Chromatin immunoprecipitation (ChIP) is a key technique for mapping histone modifications and transcription factor binding on DNA and thereby unraveling the role of epigenetics in the regulation of gene expression. Current ChIP protocols require extensive sample handling and large numbers of cells (5-10 million). primarily owing to ample loss of material during the procedure. We altered critical steps of conventional ChIP to develop a quick and quantitative (Q2) ChIP assay suitable for cell numbers 100- to 1000-fold lower than those required for conventional ChIP. Key modifications of the ChIP procedure include (i) formaldehyde DNA–protein cross-linking in suspended cells, (ii) cross-linking in the presence of 20 mM sodium butyrate to enhance specificity of precipitation of acetylated histones, (iii) transfer of washed precipitated immune complexes to a clean tube ('tube shift') to increase ChIP specificity by virtually eliminating nonspecifically bound chromatin, and (iv) combination of cross-link reversal, protein digestion, and DNA elution into a single 2-h step. We used Q2ChIP to monitor changes in 6 histone H3 modifications on the human developmentally regulated genes OCT4 (POU5F1), NANOG, and LMNA (lamin A) in the context of retinoic acid (RA)-mediated differentiation of embryonal carcinoma cells and upon reprogramming of kidney epithelial 293T cells to pluripotency in carcinoma cell extract (Taranger et al. 2005 Mol. Biol. Cell 16, 5719–5735). Real-time PCR analysis of precipitated DNA unravels an unexpected two-step heterochromatin assembly elicited by RA on the OCT4 proximal promoter, proximal enhancer, and distal enhancer, and on the NANOG promoter, whereby methylation of H3K9 and H3K27 is followed by H3K9 deacetylation. H3K4 di- and trimethylation remain relatively unaffected by RA treatment. In contrast, reprogramming of 293T cells in carcinoma extract promotes assembly of histone marks characteristic of transcriptional induction of OCT4 and NANOG, such as acetylation and demethylation of H3K9. The results argue toward ordered chromatin repackaging at developmentally regulated promoters upon differentiation or, conversely, nuclear reprogramming to pluripotency.

scholarly journals Effects of Biochar on Soluble Sugar Content in Peach Seedlings

2019 ◽  
Vol 136 ◽  
pp. 07010
Author(s):  
Jing Sun ◽  
Zhiyu Li ◽  
Jinpeng Zhu ◽  
Yuxi Wang ◽  
Tonghao Cui ◽  
...  
Keyword(s):  
Wheat Straw ◽  
Plant Height ◽  
Sugar Content ◽  
Soluble Sugar ◽  
Stem Diameter ◽  
Corn Straw ◽  
Paddy Straw ◽  
Soluble Sugar Content ◽  
Rape Straw ◽  
Significant Difference

A pot experiment was conducted to study the effects of different biochar types [addition of rape straw biochar (RB), addition of paddy straw biochar (PB), addition of wheat straw biochar (WB) and addition of corn straw biochar (MB)] on the soluble sugar content and plant height of peach seedlings. The result showed that the soluble sugar content in roots and leaves of peach seedlings treated with biochar was lower than the control. However, the soluble sugar content in stems was higher than control compared with the control. In the soluble sugar content in the aboveground part, there was no significant difference between the treatment of adding biochar and the control except for the treatment of PB. All the treatment of adding biochar increased the plant height of peach seedlings except the treatment of MB than the control and the treatment of RB and PB were at a higher level. Compared to control, all biochar addition treatments increased stem diameter of peach seedlings and the treatment of RB and PB were at a higher level. Therefore, the treatment of RB and PB was beneficial to the growth of peach seedlings.

scholarly journals Effects of storage temperature on the physiological characteristics and vegetative propagation of desiccation-tolerant mosses

2018 ◽  
Vol 15 (3) ◽  
pp. 797-808 ◽  
Author(s):  
Yuewei Guo ◽  
Yunge Zhao
Keyword(s):  
Vegetative Propagation ◽  
Storage Temperature ◽  
Sugar Content ◽  
Soluble Sugar ◽  
Storage Period ◽  
Physiological Characteristics ◽  
Regenerative Capacity ◽  
Moss Crusts ◽  
Significant Difference ◽  
Mda Content

Abstract. Mosses, as major components of later successional biological soil crusts (biocrusts), play many critical roles in arid and semiarid ecosystems. Recently, some species of desiccation-tolerant mosses have been artificially cultured with the aim of accelerating the recovery of biocrusts. Revealing the factors that influence the vegetative propagation of mosses, which is an important reproductive mode of mosses in dry habitats, will benefit the restoration of moss crusts. In this study, three air-dried desiccation-tolerant mosses (Barbula unguiculata, Didymodon vinealis, and Didymodon tectorum) were hermetically sealed and stored at five temperature levels (0, 4, 17, 25, and 30 °C) for 40 days. Then, the vegetative propagation and physiological characteristics of the three mosses were investigated to determine the influence of storage temperature on the vegetative propagation of desiccation-tolerant mosses and the mechanism. The results showed that the vegetative propagation of the three mosses varied with temperature. The most variation in vegetative propagation among storage temperatures was observed in D. tectorum, followed by the variation observed in B. unguiculata. In contrast, no significant difference in propagation among temperatures was found in D. vinealis. The regenerative capacity of the three mosses increased with increasing temperature from 0 to 17 °C, accompanied by a decrease in malondialdehyde (MDA) content, and decreased thereafter. As the temperature increased, the chlorophyll and soluble protein contents increased in B. unguiculata but decreased in D. vinealis and D. tectorum. As to storage, the MDA and soluble sugar contents increased after storage. The MDA content of the three mosses increased at each of the investigated temperatures by more than 50 % from the initial values, and the soluble sugar content became higher than before in the three mosses. The integrity of cells and cell membranes is likely the most important factor influencing the vegetative propagation of desiccation-tolerant mosses. A 40-day storage period caused cell injury. Our results suggest that storage temperature can enhance or suppress such injury and change the regenerative capacity of the three mosses. The data indicate that the suitable storage temperature is 4 °C for B. unguiculata and 17 °C for both D. vinealis and D. tectorum.

scholarly journals Effects of L-Theanine on Posttraumatic Stress Disorder Induced Changes in Rat Brain Gene Expression

2014 ◽  
Vol 2014 ◽  
pp. 1-10 ◽  
Author(s):  
Tomás Eduardo Ceremuga ◽  
Stephanie Martinson ◽  
Jason Washington ◽  
Robert Revels ◽  
Jessica Wojcicki ◽  
...  
Keyword(s):  
Gene Expression ◽  
Posttraumatic Stress Disorder ◽  
Posttraumatic Stress ◽  
Molecular Mechanisms ◽  
Stress Disorder ◽  
Traumatic Event ◽  
Pcr Analysis ◽  
Sprague Dawley ◽  
Significant Difference ◽  
Induced Changes

Posttraumatic stress disorder (PTSD) is characterized by the occurrence of a traumatic event that is beyond the normal range of human experience. The future of PTSD treatment may specifically target the molecular mechanisms of PTSD. In the US, approximately 20% of adults report taking herbal products to treat medical illnesses. L-theanine is the amino acid in green tea primarily responsible for relaxation effects. No studies have evaluated the potential therapeutic properties of herbal medications on gene expression in PTSD. We evaluated gene expression in PTSD-induced changes in the amygdala and hippocampus of Sprague-Dawley rats. The rats were assigned to PTSD-stressed and nonstressed groups that received either saline, midazolam, L-theanine, or L-theanine + midazolam. Amygdala and hippocampus tissue samples were analyzed for changes in gene expression. One-way ANOVA was used to detect significant difference between groups in the amygdala and hippocampus. Of 88 genes examined, 17 had a large effect size greater than 0.138. Of these, 3 genes in the hippocampus and 5 genes in the amygdala were considered significant (P<0.05) between the groups. RT-PCR analysis revealed significant changes between groups in several genes implicated in a variety of disorders ranging from PTSD, anxiety, mood disorders, and substance dependence.

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