Controlling Morphology and Functions of Cardiac Organoids by Two-Dimensional Geometrical Templates

Traditionally, tissue-specific organoids are generated as 3D aggregates of stem cells embedded in Matrigel or hydrogels, and the aggregates eventually end up a spherical shape and suspended in the matrix. Lack of geometrical control of organoid formation makes these spherical organoids limited for modeling the tissues with complex shapes. To address this challenge, we developed a new method to generate 3D spatial-organized cardiac organoids from 2D micropatterned hiPSC colonies, instead of directly from 3D stem cell aggregates. This new approach opens the possibility to create cardiac organoids that are templated by 2D non-spherical geometries, which potentially provides us a deeper understanding of biophysical controls on developmental organogenesis. Here, we designed 2D geometrical templates with quadrilateral shapes and pentagram shapes that had same total area but different geometrical shapes. Using this templated substrate, we grew cardiac organoids from human induced pluripotent stem cells (hiPSCs) and collected a series of parameters to characterize morphological and functional properties of the cardiac organoids. In quadrilateral templates, we found that increasing the aspect ratio impaired cardiac tissue 3D self-assembly, but the elongated geometry improved the cardiac contractile functions. However, in pentagram templates, cardiac organoid structure and function were optimized with a specific geometry of an ideal star shape. This study will shed a light on “organogenesis-by-design” by increasing the intricacy of starting templates from external geometrical cues to improve the organoid morphogenesis and functionality.

IR-Spectroscopic Study of Complex Formation of Nitrogen Oxides (NO, N2O) with Cationic Forms of Zeolites and the Reactivity of Adsorbed Species in CO and CH4 Oxidation

The formation of complexes and disproportionation of nitrogen oxides (NO, N2O) on cationic forms of LTA, FAU, and MOR zeolites was investigated by diffuse-reflectance IR spectroscopy. N2O is adsorbed on the samples under study in the molecular form and the frequencies of the first overtone of the stretching vibrations ν10–2 and the combination bands of the stretching vibrations with other vibrational modes for N2O complexes with cationic sites in zeolites (ν30–1 + ν10–1, ν10–1 + δ0–2) are more significantly influenced by the nature of the zeolite. The presence of several IR bands in the region of 2400–2600 cm−1 (the ν10–1 + δ0–2 transitions) for different zeolite types was explained by the availability of different localization sites for cations in these zeolites. The frequencies in this region also depend on the nature of the cation (its charge and radius). The data can be explained by the specific geometry of the N2O complex formed, presumably two-point adsorption of N2O on a cation and a neighboring oxygen atom of the framework. Adsorption of CO or CH4 on the samples with preliminarily adsorbed N2O at 20–180 °C does not result in any oxidation of these molecules. NO+ and N2O3 species formed by disproportionation of NO are capable of oxidizing CO and CH4 molecules to CO2, whereas NOx is reduced simultaneously to N2 or N2O. The peculiarities in the behavior of cationic forms of different zeolites with respect to adsorbed nitrogen oxides determined by different density and localization of cations have been established.

Subspace Detours Meet Gromov–Wasserstein

In the context of optimal transport (OT) methods, the subspace detour approach was recently proposed by Muzellec and Cuturi. It consists of first finding an optimal plan between the measures projected on a wisely chosen subspace and then completing it in a nearly optimal transport plan on the whole space. The contribution of this paper is to extend this category of methods to the Gromov–Wasserstein problem, which is a particular type of OT distance involving the specific geometry of each distribution. After deriving the associated formalism and properties, we give an experimental illustration on a shape matching problem. We also discuss a specific cost for which we can show connections with the Knothe–Rosenblatt rearrangement.

Fractional order plasma modeling based on linear polarization of LASER light: an Atangana–Baleanu Caputo approach

PurposeThe purpose of this offered research is to articulate a multifaceted kind of highly unstable initial perturbation and further analyze the performance of the plasma particles for time-fractional order evaluation.Design/methodology/approachFor this purpose, the authors designed specific geometry and further interpreted it into the mathematical model using the concepts of the Vlasov Maxwell system. The suggested algorithm is based on the finite-difference and spectral estimation philosophy. The management of time and memory in generic code for computational purposes is also discussed.FindingsThe main purpose is to analyze the fractional behavior of plasma particles and also the capability of the suggested numerical algorithm. Due to initial perturbations, there are a lot of sudden variations that occurred in the formulated system. Graphical behavior shows that SR parameter produces devastation as compared to others. The variation of fractional parameter between the defend domain demonstrates the hidden pictures of plasma particles. The design scheme is efficient, convergent and has the capability to cover the better physics of the problem.Practical implicationsPlasma material is commonly used in different areas of science. Therefore, in this paper, the authors increase the capability of the mathematical plasma model with specific geometry, and further suitable numerical algorithm is suggested with detailed physical analysis of the outcomes. The authors gave a new direction to study the performance of plasma particles under the influence of LASER light.Originality/valueIn the recent era, science has produced a lot of advancements to study and analyze the physical natural process, which exist everywhere in the real word. On behalf of this current developments, it is now insufficient to study the first-order time evaluation of the plasma particles. One needs to be more precise and should move toward the bottomless state of it, that is, macroscopic and microscopic time-evaluation scales, and it is not wrong to say that there exits a huge gap, to study the time evaluation in this discussed manner. The presented study is entirely an advanced and efficient way to investigate the problem into the new directions. The capability of the proposed algorithm and model with fractional concepts can fascinate the reader to extend to the other dimensions.

Low Threshold Microlasers Based on Organic-Conjugated Polymers

Conjugated polymers have emerged as ideal organic laser materials for the excellent optoelectrical properties and facile processability. During a typical lasing process, resonator configurations with specific geometry are essential to provide optical feedback and then amplified light. Herein, we summarized the geometry and working mechanism of several typical resonator configurations formed with conjugated polymers. Meanwhile, recent advances in fabrication techniques and lasing performance are also discussed to provide new ideas for the design and optimization of microcavity geometries. Followed by the advances of practical applications in fields of laser sensing, bioimaging, and laser illumination/display, we make a summary of the existing bottlenecks and future perspectives of electrically driven organic lasers toward laser display and illumination.

Recent Developments of Noise Attenuation Using Acoustic Barriers for a Specific Edge Geometry

The aim of this research is to provide a better prediction for noise attenuation using thin rigid barriers. In particular, the paper presents an analysis on four methods of computing the noise attenuation using acoustic barriers: Maekawa-Tatge formulation, Kurze and Anderson algorithm, Menounou formulation, and the general prediction method (GPM-ISO 9613). Accordingly, to improve the GPM, the prediction computation of noise attenuation was optimized for an acoustic barrier by considering new effects, such as attenuation due to geometrical divergence, ground absorption-reflections, and atmospheric absorption. The new method, modified GPM (MGPM), was tested for the optimization of an y-shape edge geometry of the noise barrier and a closed agreement with the experimental data was found in the published literature. The specific y-shape edge geometry of the noise barrier contributes to the attenuation due to the diffraction phenomena. This aspect is based on the Kirchhoff diffraction theory that contains the Huygens-Fresnel theory, which is applied to a semi-infinite acoustic barrier. The new method MGPM of predicting the noise attenuation using acoustic barriers takes into consideration the next phenomena: The effect of the relative position of the receiver, the effect of the proximity of the source or receiver to the midplane of the barrier, the effect of the proximity of the receiver to the shadow boundary, the effect of ground absorption-reflections, the effect of atmospheric absorption, and the meteorological effect due to downwind. The conclusion of the paper reveals the optimization of the method for computing the noise attenuation using acoustic barriers, including the necessary corrections for ISO-9613 and the Sound PLAN software, as well as the optimization on a case study of a specific geometry of the edge barrier.

Mode coupling and locking of a Π-shaped cantilever resonator using laser-induced asymmetric modulation

Mechanical resonators, such as microcantilevers, demonstrate significant potential for use in information technology. Cantilevered beams of various geometries clamped at one end form the most ubiquitous structures in microelectromechanical systems (MEMSs) that support multimode vibration for the detection, conversion, and processing of small signals. In this study, we demonstrate that the potential of these devices can be further extended by utilizing a strategy based on mode coupling and locking induced by asymmetric photothermal modulation. A cantilever was designed to have a Π-shape with a specific geometry such that the resonant frequencies of the two orthogonal modes are close to one another. Additionally, we show that mode coupling between the two modes, which are originally orthogonal to one another, can be achieved through laser-induced photothermal modulation. In particular, the two modes can be parametrically tuned to become degenerate through mode coupling with a significant increase in the quality factor from 112 to 839. This approach is universal and can be extended to improve the detection limits of microresonators in high-dissipation environments with enhanced signal-to-noise ratios.

Effect of Steel Fibre Reinforcement on Flexural Fatigue Behaviour of Notched Structural Concrete

One of the biggest challenges in facilitating the installation of concrete is the development of fibre-reinforced concrete. Although nowadays fibre reinforced concrete is relatively common, it is still necessary to deepen in the study on its behaviour, especially regarding its fatigue behaviour. This paper proposes a new methodology to analyse the bending fatigue behaviour of notched test specimens. From these tests, it was possible to verify that, despite carrying out the tests with load control, the presence of fibres extends the fatigue life of the concrete after cracking. This effect is of great importance since during the extra lifetime with the cracked concrete, the damage to the concrete will be evident and the corresponding maintenance measures can be carried out. Regarding the analysis of the results, in addition to obtaining a traditional S-N curve, two new criteria have been applied, namely energy and notch growth. From these two new approaches, it was possible to determine critical energy values that can be used as predictive indicators of the collapse of the element. Moreover, from the notch growth analysis, it was possible to determine crack growth rate as a function of the stress conditions for the concrete and the specific geometry. From the comparison among the results obtained from the different tests, a limit cracking index of 0.05 mm can be defined.

Design of SiC-Doped Piezoresistive Pressure Sensor for High-Temperature Applications

Within these studies the piezoresistive effect was analyzed for 6H-SiC and 4H-SiC material doped with various elements: N, B, and Sc. Bulk SiC crystals with a specific concentration of dopants were fabricated by the Physical Vapor Transport (PVT) technique. For such materials, the structures and properties were analyzed using X-ray diffraction, SEM, and Hall measurements. The samples in the form of a beam were also prepared and strained (bent) to measure the resistance change (Gauge Factor). Based on the results obtained for bulk materials, piezoresistive thin films on 6H-SiC and 4H-SiC substrate were fabricated by Chemical Vapor Deposition (CVD). Such materials were shaped by Focus Ion Beam (FIB) into pressure sensors with a specific geometry. The characteristics of the sensors made from different materials under a range of pressures and temperatures were obtained and are presented herewith.