Studies on the level of neutralizing antibodies produced by inactivated COVID-19 vaccines in the real world

Background Although effective vaccines have been developed against COVID-19, the level of neutralizing antibodies (Nabs) induced after vaccination in the real world is still unknown. To evaluate the level and persistence of NAbs induced by two inactivated COVID-19 vaccines in China. Methods and findings Serum samples were collected from 1,335 people aged 18 and over who were vaccinated with COVID-19 inactivated vaccine in Peking University People's Hospital from January 19 to June 23, 2021, for detection of COVID-19 antibodies. The WHO standard of SARS-CoV-2 NAbs was detected. The coefficients of variation between the detection results and the true values of the NAbs detected by the WHO standard were all lower than the WHO international standard 3% after the dilution of the original and the dilution of the theoretical concentrations of 500 IU/mL, 250 IU/mL, 125 IU/mL, 72.5 IU/mL, 36.25 IU/mL and 18.125 IU/mL. On day 11-70, the positive rate of NAbs against COVID-19 was 82% to 100%; From day 71 to 332, the positive rate of NAbs decreased to 27%. The level of NAbs was significantly higher at 3-8 Weeks than at 0-3 Weeks. There was a high linear correlation between NAbs and IgG antibodies in 1335 vaccinated patients. NAbs levels were decreased in 31 of 38 people (81.6%) at two time points after the second dose of vaccine. There was no significant difference in age between the group with increased and decreased neutralizing antibody levels (x2 =-0.034, P>0.05). The positive rate of NAbs in the two-dose vaccine group (77.3%) was significantly higher than that in the one-dose group (18.1%), with statistical difference (x2=312.590, P<0.001). A total of 206 people who were 11-70 days after receiving the second dose were tested and divided into three groups: 18-40 years old, 41-60 years old and >60 years old. The positive rates of NAbs in three groups (18-40 years old, 41-60 years old and >60 years old) were 95.14%, 78.43% and 81.8%, respectively. The positive rate of NAbs was significantly higher in 18-40 years old than in 41-60 years old (x2=12.547, P <0.01). The titer of NAbs in 18-40 years old group was significantly higher than that in 41-60 years old group (t=-0.222, P <0.01). The positive rate of NAbs in male group (89.32%) was lower than in female (91.26%), but there was no significant difference (x2=0.222, P >0.05). Conclusions The positive rate of NAbs was the highest from 10 to 70 days after the second dose of vaccine, and the positive rate gradually decreased as time went by. There was a high linear correlation between COVID-19 NAbs and IgM/IgG antibodies in vaccinators, suggesting that in cases where NAbs cannot be detected, IgM/IgG antibodies can be detected instead. The level of NAbs produced after vaccination was affected by age, but not by gender. The highest levels of NAbs were produced between shots 21 to 56 days apart, suggesting that 21 to 56 days between shots is suitable for vaccination.

Regression models for predicting physical and EQD2 plan parameters of two methods of hybrid planning for stage III NSCLC

Background/purpose To establish regression models of physical and equivalent dose in 2 Gy per fraction (EQD2) plan parameters of two kinds of hybrid planning for stage III NSCLC. Methods Two kinds of hybrid plans named conventional fraction radiotherapy & stereotactic body radiotherapy (C&S) and conventional fraction radiotherapy & simultaneous integrated boost (C&SIB) were retrospectively made for 20 patients with stage III NSCLC. Prescription dose of C&S plans was 2 Gy × 30f for planning target volume of lymph node (PTVLN) and 12.5 Gy × 4f for planning target volume of primary tumor (PTVPT), while prescription dose of C&SIB plans was 2 Gy × 26f for PTVLN and sequential 2 Gy × 4f for PTVLN combined with 12.5 Gy × 4f for PTVPT. Regression models of physical and EQD2 plan parameters were established based on anatomical geometry features for two kinds of hybrid plans. The features were mainly characterized by volume ratio, min distance and overlapping slices thickness of two structures. The possibilities of regression models of EQD2 plan parameters were verified by spearman’s correlation coefficients between physical and EQD2 plan parameters, and the influence on the consistence of fitting goodness between physical and EQD2 models was investigated by the correlations between physical and EQD2 plan parameters. Finally, physical and EQD2 models predictions were compared with plan parameters for two new patients. Results Physical and EQD2 plan parameters of PTVLN CI60Gy have shown strong positive correlations with PTVLN volume and min distance(PT to LN), and strong negative correlations with PTVPT volume for two kinds of hybrid plans. PTV(PT+LN) CI60Gy is not only correlated with above three geometry features, but also negatively correlated with overlapping slices thickness(PT and LN). When neck lymph node metastasis was excluded from PTVLN volume, physical and EQD2 total lung V20 showed a high linear correlation with corrected volume ratio(LN to total lung). Meanwhile, physical total lung mean dose (MLD) had a high linear correlation with corrected volume ratio(LN to total lung), while EQD2 total lung MLD was not only affected by corrected volume ratio(LN to total lung) but also volume ratio(PT to total lung). Heart D5, D30 and mean dose (MHD) would be more susceptible to overlapping structure(heart and LN). Min distance(PT to ESO) may be an important feature for predicting EQD2 esophageal max dose for hybrid plans. It’s feasible for regression models of EQD2 plan parameters, and the consistence of the fitting goodness of physical and EQD2 models had a positive correlation with spearman’s correlation coefficients between physical and EQD2 plan parameters. For total lung V20, ipsilateral lung V20, and ipsilateral lung MLD, the models could predict that C&SIB plans were higher than C&S plans for two new patients. Conclusion The regression models of physical and EQD2 plan parameters were established with at least moderate fitting goodness in this work, and the models have a potential to predict physical and EQD2 plan parameters for two kinds of hybrid planning.

Transportation container for pre-processing cytogenetic assays in radiation accidents

We report a shipping container that enables a disruptive logistics for cytogenetic biodosimetry for radiation countermeasures through pre-processing cell culture during transportation. The container showed precise temperature control (< 0.01 °C) with uniform sample temperature (< 0.1 °C) to meet the biodosimetry assay requirements. Using an existing insulated shipping box and long shelf life alkaline batteries makes it ideal for national stockpile. Dose curve of cytogenetic biodosimetry assay using the shipping container showed clear dose response and high linear correlation with the control dose curve using a laboratory incubator (Pearson’s correlation coefficient: 0.992). The container’s ability of pre-processing biological samples during transportation could have a significant impact on radiation countermeasure, as well as potential impacts in other applications such as biobanking, novel molecular or cell-based assays or therapies.

Correlation between polychromatic image quality metrics to predict visual acuity in pseudophakic patients

The optical quality of three multifocal intraocular lenses (MIOLs) was measured in vitro using the area under the modulation transfer function (MTFa) and energy efficiency (EE). We evaluated those metrics through-focus (TF) by axially scanning the image space with three wavelengths (R, G, B). High linear correlation between MTFa and EE was found for all cases. Correlations between optical in vitro results (polychromatic TF-MTFa and TF-EE) and clinical visual acuity (VA) defocus curves for the three MIOLs allowed us to derive mathematical expressions. Both metrics proved to be equivalent to predict postoperative VA.

The Design and Application of Simplified Insole-Based Prototypes with Plantar Pressure Measurement for Fast Screening of Flat-Foot

This study aimed to find the correlation between conventional Arch Index (AI) measurements and our prototype of a simplified insole-based plantar pressure measurement system and to find out the effective plantar pressure sensor position. Twenty-one subjects participated in this study, which was divided into two groups: 10 subjects with flatfoot and 11 subjects with normal foot. Five force sensitive resistance sensors were used on this prototype using Arduino as the data acquisition device. Two types of trials, namely static and dynamic, were conducted to validate our system against the ink-type AI measurement as a golden standard. The results showed that in the static trial, there was a high linear correlation with the medial arch sensor configuration, while in the dynamic trial, there was a high linear correlation in the medial arch sensor configuration and sensor 5 configuration. This study showed that both static and dynamic tests using the self-developed device could effectively determine most of the flatfoot subjects and suggests that in the future, it can be applied in clinical applications because of its advantages when compared to the expensive-high tech graphic input board and conventional tools, like ink-type based measurements.

FWHM Dimentional Analysis From Scattered Light Intensity Profile for Dry Rubber Content Determination in Natural Rubber

Dry Rubber Content (DRC) describes a rubber particle percentage in natural rubber latex. In this paper, the relation between forward light scattering profiles of natural latex and rubber contents is reported for dry rubber content latex. The profile, characterized by Full Width at Half Maximum (FWHM), is increasing linearly with respect to rubber content. The measurement was performed immediately after latex being tapped with necessary addition of acetic acid. This addition was meant to prevent latex coagulation. There is a high linear correlation between DRC and FWHM of both domain: one and two dimension. This is indicated by correlation factor which are higher than 0.9 for both of domains and sufficient in DRC determination.

Effect of Fabric Structure and Weft Density on the Poisson's Ratio of Worsted Fabric

This paper investigates the impacts of weave structures and weft density on the Poisson's ratio of worsted fabric under uniaxial extension. In this study nine groups of worsted fabrics comprising of three weave structures (twill 2/2, twill 3/1 and hopsack 2/2), each produced in three different weft densities were examined. Samples were extended in weft direction uniaxially and the Poisson's ratio of fabric in various extensions was measured. Analysis showed that the effect of both weft density and weave structure are significant with no combination effect on the Poisson's ratio. It was found that there is an exponential correlation between warp and weft crimp during fabric extension. For the worsted fabrics used in this research in all three fabric structures, fabrics with higher weft yarn density have higher value of Poisson's ratio. It was also concluded that for the fabrics with the same condition but only different in structures, this ratio is related to the structural firmness of fabric. In all three fabric structures the value of the Poisson's ratio were following the same pattern of twill 2/2, twill 3/1 and hopsack 2/2 from highest to lowest value. It was revealed that there is a high linear correlation between the crimp interchange ratio and Poisson's ratio.

Comparison of Mobile-Radar Measurements of Tornado Intensity with Corresponding WSR-88D Measurements

In the United States, visual observations of tornadoes and/or the existence of tornado damage currently provide the sole evidence of tornadogenesis in association with a mesocyclone or other radar-detected storm-scale vortex. The severity of the tornado damage is currently the only means of estimating the intensity of tornadoes, radar detected or otherwise. The limitations of the damage-based record of tornado occurrence and intensity are well known and motivated this research. Weather Surveillance Radar-1988 Doppler (WSR-88D) measurements of the translating tornadic flow were compared with (semi-) coordinated measurements obtained near the surface with mobile radar. On the basis of a small yet fairly broad sample of tornadoes, high linear correlation was found between the vortex intensity (rotation plus translation) quantified using WSR-88D data and that quantified using Doppler on Wheels data. The possible effects of Doppler radar sampling on these results were explored through experiments with a simple vortex model. These experiments argued that the likelihood is high that a tornado would be sampled in a favorable way during at least one radar scan. Hence, the suggestion from this work is that WSR-88Ds (or similar operational radars) can potentially be used in isolation to estimate low-level tornado intensity. The proposed estimation is by way of a linear regression model, and application of this model is relevant only once a tornado is already confirmed.

The Motion Analysis System and The Maximal Area of Fingertip Motion

We have used the motion analysis system to evaluate the maximal area of fingertip motion. Some modification in setting the cameras and use of a smaller marker is required. In this series, 58 examinations have been accomplished on 28 fingers with various traumatic injuries. The closed curve derived from the motion analysis system and the area calculated from it were easier to interpret and could be compared in serial examinations. A high linear correlation between the fingertip motion area and total active motion was found. The computer-aided motion analysis system complements the traditional methods of assessing an injured finger.