scholarly journals Microscopic Profile of Mice Liver Tissue (Mus musculus) Fixed with Neutral Buffered Formalin (NBF 10%) and Helly Solution

2021 ◽  
Vol 3 (2) ◽  
pp. 90-94
Author(s):  
Melly Agustin
Keyword(s):  
Experimental Study ◽  
Mus Musculus ◽  
Descriptive Analysis ◽  
Tissue Preparation ◽  
Long Time ◽  
Microscopic Picture ◽  
Mice Liver ◽  
Good Preparation ◽  
Anatomical Pathology ◽  
Better Than

The fixation solution that is widely used in anatomical pathology laboratories is NBF 10%, the excess of NBF 10% because the pH is close to neutral, can be stored in large quantities and a long time. Helly's fixation solution is a good fixation solution for the cytoplasm, and only requires 2-3 hours of fixation. Knowing the microscopic picture of the preparation of hepar mencit tissue (Mus musculus) fixated with NBF 10% and Helly solution. This research is an experimental study with a descriptive analysis approach. picture of hepar mencit tissue preparation (Mus musculus) fixation with NBF 10% obtained as much as 100% good preparation. While the fixated with Helly solution obtained as much as 66% good preparation. Conclusion: Microscopic picture of hepar mencit tissue preparation (Mus musculus) fixated with NBF is 10% better than Helly solution.

scholarly journals EFEK KONSUMSI KERANG BULU (Anadara antiquata) TERHADAP KUANTITAS DAN KUALITAS SPERMATOZOA

2019 ◽  
Vol 12 (2) ◽  
pp. 207-219
Author(s):  
Neny Safitri ◽  
Evi Hanizar
Keyword(s):  
Experimental Study ◽  
Mus Musculus ◽  
Motility Of Sperm ◽  
Better Than

Kerang Bulu (Anadara antiquata) merupakan salah satu makanan yang mengandung nutrisi tinggi seperti protein, lemak, vitamin dan mineral yang berpotensi memperbaiki serta meningkatkan kualitas spermatozoa. Penelitian ini bertujuan untuk menganalisis efek kerang bulu (A.antiquata) terhadap kuantitas dan kualitas spermatozoa Mus musculus. Penelitian ini merupakan penelitian eksperimental murni dengan rancangan acak lengkap pola faktorial, faktor pertama kondisi kerang  yakni mentah dan kukus, faktor kedua konsentrasi 0%(kontrol), 75% dan 100%. M. musculus  yang digunakan sebanyak 25 ekor terdiri dari limAbstrakKerang bulu (Anadara antiquata) merupakan salah satu makanan yang mengandung nutrisi tinggi, seperti protein, lemak, vitamin, dan mineral, yang berpotensi memperbaiki serta meningkatkan kualitas spermatozoa. Penelitian ini bertujuan untuk menganalisis efek A. antiquata terhadap kuantitas dan kualitas spermatozoa Mus musculus. Penelitian ini merupakan penelitian eksperimental murni dengan rancangan acak lengkap pola faktorial. Faktor pertama adalah kondisi kerang, yakni mentah dan kukus, dan faktor kedua adalah konsentrasi 0 g/mL, 75 g/100 mL, dan 100 g/100 mL. M. musculus yang digunakan sebanyak 25 ekor, terdiri dari lima perlakuan dengan masing-masing lima kali ulangan. Parameter yang diamati adalah konsentrasi (kuantitas), motilitas, dan morfologi (kualitas) spermatozoa. Data dianalisis menggunakan uji Kruskal-Wallis dan uji lanjut Duncan. Hasil penelitian menunjukkan bahwa A. antiquata berpengaruh nyata terhadap konsentrasi dan motilitas spermatozoa, sedangkan morfologi tidak memberikan pengaruh yang nyata. Kondisi kerang mentah dengan konsentrasi 100 g/100 mL memberikan efek rata-rata terendah terhadap konsentrasi dan motilitas spermatozoa, sementara kerang kukus dengan konsentrasi 75 g/100 mL meningkatkan rata-rata konsentrasi dan motilitas spermatozoa tertinggi. Penelitian ini memberi kesimpulan bahwa mengonsumsi kerang kukus lebih baik untuk meningkatkan konsentrasi dan motilitas spermatozoa dibandingkan dalam kondisi mentah. Peningkatan dosis kerang kukus tidak linier terhadap respon kuantitas dan kualitas spermatozoa.Kata kunci: Anadara antiquata; Konsentrasi spermatozoa; Motilitas; Morfologi; Mus musculusAbstract Shellfish (Anadara antiquata) was one of the foods that contained high nutrients such as protein, fat, vitamins, and minerals that had the potential to improve and increase the quality of sperm. The study aims to analyze the effect of the shellfish on the quantity and quality of Mus musculus sperm. This study was a purely experimental study with a completely randomized factorial pattern; the first factor was the condition of shellfish which was raw and steamed, and the second factor was concentrations of 0 g/mL, 75 g/100 mL and 100 g/100 mL. Twenty-five individuals of M. musculus were subject to five treatments, each with five replications. Parameters observed were concentration, motility, and morphology of sperm. Data were analyzed using the Kruskal-Wallis test and Duncan's advanced test. The results showed that shellfish had a significant effect on the concentration and motility of sperm, whereas there was no significant effect on morphology. The dose which gave the effect was 100 g/100 mL of raw shellfish and 75 g/100 mL of steamed shellfish. This study concluded that consuming steamed shellfish was better than raw for increasing the concentration and motility of sperm. The shellfish dose increased is not linear in response to the quantity and quality of sperm.a perlakuan masing-masing lima kali ulangan. Parameter yang diamati jumlah (kuantitas), motilitas dan morfologi (kualitas) spermatozoa. Data dinalisis menggunakan uji Kruskal-wallis dan uji lanjut Duncan. Hasil penelitian menunjukkan kerang bulu (A.antiquata ) pengaruh nyata terhadap jumlah dan motilitas spermatozoa sedangkan pada morfologi  tidak memberikan pengaruh yang nyata. Kondisi kerang mentah dengan konsentrasi 100g/100 ml air memberikan efek rata-rata terendah terhadap jumlah dan motilitas spermatozoa, sementara kerang kukus dengan konsentrasi 75g/100 ml meningkatkan rata-rata jumlah dan motilitas spermatozoa tertinggi. Penelitian ini memberi kesimpulan mengkonsumsi kerang kukus lebih baik untuk meningkatkan jumlah dan motilitas spermatozoa dibanding dalam kondisi mentah. Peningkatan dosis tidak linier terhadap respon kuantitas dan kualitas spermatozoa. 

Risk Management on Information Security in ABC Company

2017 ◽  
Vol 4 (1) ◽  
pp. 62-66
Author(s):  
Luyen Ha Nam
Keyword(s):  
Risk Management ◽  
Information Security ◽  
Data Management ◽  
Management System ◽  
Risk Mitigation ◽  
Data Management System ◽  
Data Centre ◽  
Long Time ◽  
Mitigation Action ◽  
Better Than

From long, long time ago until nowadays information still takes a serious position for all aspect of life, fromindividual to organization. In ABC company information is somewhat very sensitive, very important. But how wekeep our information safe, well we have many ways to do that: in hard drive, removable disc etc. with otherorganizations they even have data centre to save their information. The objective of information security is to keep information safe from unwanted access. We applied Risk Mitigation Action framework on our data management system and after several months we have a result far better than before we use it: information more secure, quickly detect incidents, improve internal and external collaboration etc.

DESAIN PEMBELAJARAN BAHASA INDONESIA BERBASIS PEMAHAMAN NILAI SIKAP GOTONG ROYONG

2020 ◽  
Vol 13 (2) ◽  
pp. 01-10
Author(s):  
Alpansyah Alpansyah ◽  
Abdul Talib Hasim
Keyword(s):  
Experimental Study ◽  
Language Learning ◽  
Reader Response ◽  
Female Students ◽  
Male Students ◽  
Mutual Cooperation ◽  
Male And Female ◽  
Quasi Experimental ◽  
Bahasa Indonesia ◽  
Better Than

The aims of this study were: (1) to identify an increase in students' understanding of the value of mutual cooperation through the use of reader response rules in Indonesian Language Learning (KRPDPBI); (2) identifying the use of the reader response principle in Indonesian Language learning (KRPDPBI) there are differences between male and female students. The design of this study used a quasi-experimental study with two different methods. The results showed that (1) the achievement of the score of understanding the value of mutual cooperation for students taught by KRPDPBI was better than for students taught by regular learning according to the curriculum; (2) the achievement of the understanding of the value of male students' mutual cooperation is no better than that of female students.

scholarly journals Inhibition of CCL7 derived from Mo-MDSCs prevents metastatic progression from latency in colorectal cancer

2021 ◽  
Vol 12 (5) ◽  
Author(s):  
Xiaoli Ren ◽  
Jianbiao Xiao ◽  
Wanning Zhang ◽  
Feifei Wang ◽  
Yongrong Yan ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Suppressor Cells ◽  
Metastatic Progression ◽  
Ht29 Cells ◽  
Metastatic Cells ◽  
Long Time ◽  
Mice Liver ◽  
Short Time ◽  
Related Proteins

AbstractIn colorectal cancer (CRC), overt metastases often appear after years of latency. But the signals that cause micro-metastatic cells to remain indolent, thereby enabling them to survive for extended periods of time, are unclear. Immunofluorescence and co-immunoprecipitation assays were used to explore the co-localization of CCL7 and CCR2. Immunohistochemical (IHC) assays were employed to detect the characters of metastatic HT29 cells in mice liver. Flow cytometry assays were performed to detect the immune cells. Bruberin vivo MS FX Pro Imager was used to observe the liver metastasis of CRC in mice. Quantitative real-time PCR (qRT-PCR) and western blot were employed to detect the expressions of related proteins. Trace RNA sequencing was employed to identify differentially expressed genes in MDSCs from liver micro-M and macro-M of CRC in mice. Here, we firstly constructed the vitro dormant cell models and metastatic dormant animal models of colorectal cancer. Then we found that myeloid-derived suppressor cells (MDSCs) were increased significantly from liver micro-metastases to macro-metastases of CRC in mice. Moreover, monocytic MDSCs (Mo-MDSC) significantly promoted the dormant activation of micro-metastatic cells compared to polymorphonuclear MDSCs (PMN-MDSC). Mechanistically, CCL7 secreted by Mo-MDSCs bound with membrane protein CCR2 of micro-metastatic cells and then stimulated the JAK/STAT3 pathway to activate the dormant cells. Low-dose administration of CCL7 and MDSCs inhibitors in vivo could significantly maintain the CRC metastatic cells dormant status for a long time to reduce metastasis or recurrence after radical operation. Clinically, the level of CCL7 in blood was positively related to the number of Mo-MDSCs in CCR patients, and highly linked with the short-time recurrence and distant metastasis. CCL7 secreted by Mo-MDSCs plays an important role in initiating the outgrowth of metastatic latent CRC cells. Inhibition of CCL7 might provide a potential therapeutic strategy for the prevention of metastasis recurrence.

The Experimental Study on the Inhibition Effect of Fusarium oxyspomm with Bio-Slurry Based on Material Properties

2013 ◽  
Vol 675 ◽  
pp. 317-321
Author(s):  
Meng Ying Fang ◽  
Li Chun Liu ◽  
Fang Yin ◽  
Wu Di Zhang ◽  
Shi Qing Liu ◽  
...  
Keyword(s):  
Experimental Study ◽  
Material Properties ◽  
Water Soluble ◽  
Inhibition Mechanism ◽  
Main Subject ◽  
Soluble Substance ◽  
Inhibition Effect ◽  
Water Soluble Substance ◽  
Main Component ◽  
Better Than

Using petroleum ether to extract the fermentative fluid (bio-slurry), then to get the inhibition mechanism of it, and infer which is the main component in inhibition mechanism of biogas. The conclusion found by the experiment is that fat soluble substance is better than water soluble substance in inhibition mechanism, and fat soluble substance is close to 75% biogas fermentation fluid, while water soluble substance is worst. That is to say, the main subject in inhibition mechanism is hided in the fat soluble substance.

Experimental Study on Mechanical Property of Concrete Based on Seawater and Sea Sand

2013 ◽  
Vol 641-642 ◽  
pp. 574-577 ◽  
Author(s):  
Ying Tao Li ◽  
Ling Zhou ◽  
Mao Jiang ◽  
Yu Zhang ◽  
Jun Shao
Keyword(s):  
Mechanical Property ◽  
Experimental Study ◽  
Fly Ash ◽  
Raw Materials ◽  
Growth Trend ◽  
Sea Sand ◽  
Significant Strength ◽  
Dry Conditions ◽  
Early Strength ◽  
Better Than

In this paper, the mechanical property experiments of concrete based on the seawater and sea sand have been carried in different raw materials preparation and different conservation environments. The results show that the early strength and late strength of concrete based on seawater and sea sand are better than concrete based on freshwater and sand. There is no significant strength decreased for concrete based on seawater and sea sand under accelerated alternating wet and dry conditions. For concrete based on seawater and sea sand mixed with admixture, the downward trend of late strength is significantly delayed, the late strength of concrete based on the seawater and sea sand mixed with slag gets the most obvious growth trend, while the late strength of seawater and sea sand concrete mixed with fly ash gets the largest increment.

Experimental Study on Application of Boron Mud Secondary Resource to Oxidized Pellets Production

2017 ◽  
Vol 36 (7) ◽  
pp. 649-655
Author(s):  
Xiao-Jiao Fu ◽  
Man-Sheng Chu ◽  
Jia-Qi Zhao ◽  
Shuang-Yin Chen ◽  
Zheng-Gen Liu ◽  
...  
Keyword(s):  
Experimental Study ◽  
Blast Furnace ◽  
Furnace Operation ◽  
Blast Furnace Operation ◽  
Fundamental Study ◽  
Secondary Resource ◽  
Oxidized Pellets ◽  
Rich Material ◽  
Boron Mud ◽  
Better Than

AbstractIn order to realize comprehensive and massive treatment of boron mud secondary resource, fundamental study on boron mud applied to oxidized pellets production as additive was carried out in the paper under laboratory conditions. The effects of boron mud on the performance of oxidized pellets were investigated systemically, and boron mud was combined with other boron-rich material innovatively. The results showed that, within certain limits, boron mud can improve properties of oxidized pellets. The bentonite content decreased to 0.3 % when adding 1.0 % boron mud additive and the pellets met blast furnace requirements. With the combination additive content 0.8 %, bentonite content can be further decreased to 0.2 %, and the pellets properties were better than base pellet. Therefore, it was an effective way to reduce environmental pollution and optimize blast furnace operation by developing boron mud secondary resource as pellets additive.

Experimental Study on Earth Pressure of Corrugated Steel Culvert under High Fill Embankment

2013 ◽  
Vol 405-408 ◽  
pp. 1815-1819
Author(s):  
Wen Sheng Yu ◽  
Zhu Long Li ◽  
Xiao Ru Xie ◽  
Li Yuan Guo
Keyword(s):  
Mechanical Property ◽  
Experimental Study ◽  
Reinforced Concrete ◽  
Concrete Slab ◽  
Earth Pressure ◽  
Reinforced Concrete Slab ◽  
The Earth ◽  
Filling Height ◽  
Test Points ◽  
Better Than

To analyze the earth pressure of corrugated steel culvert under high fill embankment, a field test was taken and the change law was got with the filling height increasing, the force state when geotechnical grilles were laid on the top of corrugated steel culvert was compared to that of reinforced concrete slab culvert. Results show that the pressure on the top of corrugated steel culvert is smaller than that on the external in same level when test points are near to culvert, the values of test points above and below geotechnical grilles are close, and the pressure of corrugated steel culvert is smaller than that of reinforced concrete slab culvert when filling height is above 7.3 m. So analysis indicates corrugated steel culvert spreads the upper load better, the geotechnical grille can reduce the pressure effectively through earth pressure redistribution, and the mechanical property of corrugated steel culvert is better than reinforced concrete slab culvert under high fill embankment.

Deliberate Presleep Control of Dream Content: An Experimental Study

1977 ◽  
Vol 45 (2) ◽  
pp. 660-662 ◽  
Author(s):  
M. L. Griffin ◽  
D. Foulkes
Keyword(s):  
Experimental Study ◽  
Dream Content ◽  
Reliable Evidence ◽  
Better Than ◽  
Do So

29 subjects attempted, over a period of 10 nights, to influence their dreams using techniques described in Garfield's book, Creative Dreaming (1974). A target suggestion was selected from a list of six suggestions compiled by, or for, each subject. Subjects kept daily records during the experiment both of their efforts at dream influence and of the dreams they recalled. Four judges attempted to identify from the dream material the target suggestion on each subject's suggestion list. The results indicated that the judges were unable to do so at better than chance levels. Thus analysis indicated no reliable evidence that conscious presleep suggestions become incorporated into dream content.

scholarly journals Efektivitas Pembelajaran Kooperatif Tipe STAD Pada Materi Pokok Perbandingan Di SMPN II Megaluh Jombang

2018 ◽  
Vol 4 (2) ◽  
pp. 141-152
Author(s):  
Rifa Nurmilah
Keyword(s):  
Cooperative Learning ◽  
Learning Process ◽  
Teaching And Learning ◽  
Teaching Practice ◽  
Descriptive Analysis ◽  
Learning Outcome ◽  
Cooperative Teaching ◽  
Conventional Teaching ◽  
Quasi Experimental ◽  
Better Than

The aims of this research are: 1) to investigate the effectiveness of cooperative teaching practice using STAD type in the topic of comparison; 2). investigate whether the learning outcome of the students who were involved in cooperative learning process is better than those who were involved in conventional learning process in the topic of comparison. This research was quasi experimental research. The samples of this research were the students of VIIA as the experimental classroom and the students of VIIB as the control classroom. Based on the descriptive analysis, it was concluded that the use of cooperative learning using STAD type in the topic of comparison in SMPN II Megaluh was proven effective with criteria 1) students mastery reach 87.09%, 2) the students active participation is effective, 3) the teachers capability in managing classroom is effective, 4) the students response of the teaching and learning practice are positive. Based on the analysis by using inferential t-test, it was gained ttest= 2.197 and ttest > ttable,. It means the students learning outcome using cooperative teaching and learning process was better than those using conventional teaching and learning process in SMPN II Megaluh Jombang, academic year 2013/2014.

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