scholarly journals The Apelinergic System Immuno-Detection in the Abomasum and Duodenum of Sheep Grazing on Semi-Natural Pasture

Animals ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 3173
Author(s):  
Elisa Palmioli ◽  
Cecilia Dall’Aglio ◽  
Michele Bellesi ◽  
Federico Maria Tardella ◽  
Sara Moscatelli ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Neuroendocrine Cells ◽  
Food Supplementation ◽  
Fundic Gland ◽  
Sheep Grazing ◽  
Lower Intensity ◽  
Feed Supplementation ◽  
Natural Pasture ◽  
Group Feeding ◽  
Experimental Group

Apelin (APLN) is an adipokine mainly produced by adipose tissue and related to an individual’s nutritional status as well as digestive apparatus functions. In this work, APLN and its receptor (APLNR) were investigated, by immunohistochemistry, in the abomasum and duodenum of 15 Comisana × Appenninica adult sheep reared in a semi-natural pasture. Organ samples were collected after maximum pasture flowering (M × F group) and after maximum pasture dryness (M × D group); the experimental group (E × p group) received a feed supplementation of 600 grams/day/head of barley and corn in addition to M × D group feeding. APLN and APLNR were identified in the lining epithelium and the fundic gland chief cells of the abomasum. APLNR was observed in the lining epithelium, in the crypts and the serotonin secreting cells of the duodenum. Similar reactivity was observed between the M × F and E × p groups, while the M × D group showed a lower intensity of immunostaining for both APLN and APLNR in all positive structures but the duodenal serotonin neuroendocrine cells. Hence, our findings show that the E × p group presents a picture quite overlapped with M × F and suggest that food supplementation has a maintaining effect on the apelinergic system expression in the investigated digestive tracts of the sheep.

Correction of morphofunctional disorders with grapes polyphenols in rats with experimental metabolic syndrome

2018 ◽  
pp. 43-48
Author(s):  
Ю.И. Шрамко ◽  
А.В. Кубышкин ◽  
А.А. Давыдова ◽  
И.И. Фомочкина ◽  
Л.Л. Алиев ◽  
...  
Keyword(s):  
Metabolic Syndrome ◽  
Adipose Tissue ◽  
Visceral Adipose Tissue ◽  
Normal Structure ◽  
Hepatic Tissue ◽  
Standard Diet ◽  
Grape Polyphenols ◽  
Morphological Studies ◽  
Visceral Adipose ◽  
Experimental Group

Цель работы состояла в изучении влияния полифенолов винограда на органы-мишени при экспериментальном метаболическом синдроме у крыс. Методы. В течение 12 недель полифенолы винограда применялись у крыс линии Вистар. Все крысы находились на стандартном рационе. Животные были разделены на 6 групп: 1-я контрольная получала питьевую воду; 2-я контрольная и все 4 экспериментальные - 2,5% раствор фруктозы в качестве питья. 1-я экспериментальная группа дополнительно получала препарат «Фэнокор» с суммарным содержанием полифенолов 181,53 г/дм, 2-я экспериментальная - виноматериал с суммарным содержанием полифенолов 1,73 г/дм; 3-я экспериментальная - виноматериал с суммарным содержанием полифенолов 4,33 г/дм и 4-я экспериментальная - виноматериал с суммарным содержанием полифенолов 8,58 г/дм. После окончания опыта у крыс проводили морфологические исследования висцеральной жировой ткани, тканей миокарда и печени. Результаты. Анализ результатов показал, что применение полифенольных продуктов переработки винограда в концентрациях 181,53 г/дм при моделировании метаболического синдрома приводило к минимизации морфофункциональных нарушений в висцеральной жировой ткани (уменьшение интенсивности лимфоплазмоцитарной инфильтрации), миокарде (мышечные волокна имели типичное строение и адипоциты между ними встречались лишь очагово) и печени (имелись лишь слабые очаговые дистрофические изменения гепатоцитов). Заключение. Результаты работы свидетельствуют о возможности применения виноматериалов с наибольшей концентрацией полифенолов и препарата «Фэнокор» в коррекции и профилактике поражений при метаболическом синдроме. The aim of this work was to study the effect of grape polyphenols on target organs in rats with experimental metabolic syndrome. Methods. Grape polyphenols were used in Wistar rats for 12 weeks. All rats received a standard diet. The animals were divided into 6 groups: group 1, control, received drinking water; group 2, the second control, and four experimental groups received a 2.5% fructose solution for drinking. The first experimental group additionally received a drug, Fenocor, containing polyphenols at 181.53 g/dm; the second experimental group - wine material containing polyphenols at 1,73 g/dm; the third experimental group - wine material containing polyphenols at 4,33 g/dm; and the fourth experimental group - wine material containing polyphenols at 8,58 g/dm. At the end of experiment, morphological studies of visceral adipose tissue, myocardial tissue, and hepatic tissue were performed. Results. The treatment of rats with experimental metabolic syndrome with grape polyphenolic products at a concentration of 181.53 g/dm minimized morphological and functional disorders in visceral adipose tissue (intensity of lymphoplasmocytic infiltration was decreased), myocardium (muscle fibers had normal structure with only occasional adipocytes between them), and liver (only slight focal degenerative changes were observed in hepatocytes). Conclusion. The study indicated a possibility of using wine materials with the highest concentration of polyphenols and the drug Fenocor for correction and prevention of damages in metabolic syndrome.

scholarly journals The Influence of Coaches’ Communication on The Level of Players’ Pre-Competitive Anxiety and Selfesteem

2016 ◽  
Vol 56 (2) ◽  
pp. 129-142
Author(s):  
Peter Kačúr
Keyword(s):  
Educational Program ◽  
Self Esteem ◽  
Control Group ◽  
Intervention Period ◽  
Lower Intensity ◽  
Somatic Anxiety ◽  
Competitive Anxiety ◽  
Cognitive Anxiety ◽  
Experimental Group ◽  
Mastery Approach

Summary The purpose of the study was to evaluate the influence of coaches` communication discourse determined by educational program Mastery Approach to Coaching (MAC) on changes of players` level of pre-competitive anxiety and self-esteem. The research was applied on 10 coaches of collective sports and 161 players. For diagnosing, the level of cognitive, somatic anxiety and selfesteem was used standardized questionnaire CSAI-2R. Players of experimental group decreased level of intensity of cognitive (p = 0.004) and somatic anxiety as well as frequency and increased level of selfesteem intensity (p = 0.021) and frequency during intervention period. After three months’ experiment period players of experimental group experienced lower intensity (p = 0.000) and frequency of cognitive anxiety as well as lower intensity (p = 0.012) and frequency (p = 0.037) of somatic anxiety than players in control group. Players of experimental group perceived direction of self-esteem intrusions (p = 0.041) as well as intrusions of cognitive and somatic anxiety more positively than players of control group after intervention period.

(-)-Epicatechin reduces adiposity in male offspring of obese rats

2019 ◽  
Vol 11 (1) ◽  
pp. 37-43 ◽  
Author(s):  
Sergio De los Santos ◽  
Luis Antonio Reyes-Castro ◽  
Ramón Mauricio Coral-Vázquez ◽  
Juan Pablo Méndez ◽  
Marcela Leal-García ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Visceral Adipose Tissue ◽  
Visceral Fat ◽  
Metabolic Profile ◽  
Maternal Obesity ◽  
Male Offspring ◽  
Model Assessment ◽  
Obese Rats ◽  
Visceral Adipose ◽  
Experimental Group

AbstractObjective:To determine whether (-)-epicatechin (Epi) could decrease visceral adipose tissue and improve the metabolic profile of male offspring rats, after maternal obesity was induced by a high-fat diet (HFD).Design:Maternal obesity in albino Wistar rats was induced with a HFD, whereas male offspring were fed with chow diet throughout the study. Eight male offspring per group, from different litters, were randomly assigned to the experimental or to the control groups. In the experimental group, Epi was administered at a dose of 1 mg/kg of body weight to the male offspring twice daily for two weeks, beginning at postnatal day (PND).Main measures:Weight of visceral adipose tissue, adipocyte size, and several metabolic parameters.Results:Epi administration in the male offspring induced a significant decrease in the amount of visceral fat (11.61 g less, P < 0.05) and in the size of adipose cells (28% smaller, P < 0.01). Besides, Epi was able to decrease insulin, leptin, and Homeostasis Model Assessment -Insulin Resistance (HOMA-IR) (P < 0.05), as well as triglycerides, when the experimental group was compared to the untreated male offspring of obese rats (P < 0.01).Conclusions:Epi administration can reverse the negative effects that maternal obesity has on the male offspring. This could be because Epi reduces the amount of visceral fat and improves metabolic profile.

scholarly journals Disponibilite, Valeurs Marchande Et Nutritionnelle Des Sous-Produits Agricoles Et Agroindustriels Utilises Dans L’alimentation Des Ruminants Au Benin

2016 ◽  
Vol 12 (33) ◽  
pp. 422
Author(s):  
Montcho M. ◽  
Babatoundé S. ◽  
Aboh B. A. ◽  
Bahini M. J. D. ◽  
Chrysostome A. A. M. C. ◽  
...  
Keyword(s):  
Crude Protein ◽  
The Other ◽  
Food Supplementation ◽  
Natural Pasture ◽  
Maize Bran ◽  
Oil Cakes ◽  
Commercial Value ◽  
Cassava Peels ◽  
By Products ◽  
Very High

The availabilities and the commercial values of 31 agricultural and agro-industrial by-products were evaluated through a semi structural interview carried out on 360 breeders of Benin. A sample of each diet was collected to determine its chemical composition and nutritive values. Maize bran, cassava peels, cakes of soya and cotton are available in any season and have a commercial value. Among fruit by-products used in the food of the ruminants, the pineapple peels occupy the first place and their commercial values are three times higher than those of the cassava peels. Soya cakes has a very high commercial value (higher than 200 FCFA/kg).The leguminous pods and the cereal hulls are also largely available but without commercial value. Roots cassava and tubers of yam by-products and the cakes are very digestible and these values do not go down below 60 %. Low values of digestibility were observed on hulls and stems of cereals and leguminous pods. These digestibilities are generally below 40 %. The groundnut haulms are more digestible than cowpea haulms (57.8 vs 49.7 %). Cotton cakes and palm oil cakes are less digestible than the other oil cakes. The digestibilities of crude protein (DCP) are very low in fruit and cereals by-products. Except for the pods of leguminous, all the leguminous by-products provided considerable quantities in DCP. The studies give opportunities of choice of food supplementation to ruminants during the dry season where graminaceous and the other herbaceous becoming rare in the natural pasture.

scholarly journals Growth and development of bees using organic acids and probiotics

2016 ◽  
Vol 18 (2) ◽  
Author(s):  
I. V. Dmitruk ◽  
S. M. Suhovuha
Keyword(s):  
Lactic Acid ◽  
Citric Acid ◽  
Lactic Acid Bacteria ◽  
Research Group ◽  
Growth And Development ◽  
The Third ◽  
Group Feeding ◽  
The Difference ◽  
Bee Colonies ◽  
Experimental Group

The second record number of sealed brood, which took place on May 15 showed that consumption of succinic acid pergi helped increase the number of brood grown by 11.2% (P <0.001) and in the experimental group. Feeding bees use citric acid positively affects the amount of brood in the second experimental group. Compared with controls, the number of brood is higher by 7.5% (P < 0.001). Use of feeding bees III experimental group of lactic acid bacteria has increased the brood at 10.0% (P < 0.001). May 27 The difference between the number of counted squares and the control and experimental group is 16.3% (P <0.001), the second experimental group 11.5% (P < 0.001), the third experimental group 13.4% (P < 0.001). In the period, which fell on June 8 development of bee colonies increased, so the difference between the number of counted squares and the control and development of 22.2% (P < 0.001), the second experimental group 17.8% (P < 0.001), the third research group 18.9% (P < 0.001). June 20, recorded the best performance of the experimental group, the difference between the number of counted squares and the control and experimental group is 23.1% (P < 0.001), the second experimental group 18.3% (P < 0.001), the third experimental group 19.3 % (P < 0.001). Compared with the rate on May 3 yaytsenosnosti intensity oviposition of the uterus and research group for 27 May increased by 62.5%. Consumption pergi bees II nd experimental group caused an increase yaytsenosnosti cancer – by 56.3%, the third second experimental group – by 58.2%. Compared to control subjects in increasing the number of families found grown brood on 18,2 –23,1% (P <0.001).

scholarly journals The effect of compound feed on the productivity of broiler chickens

2021 ◽  
Vol 37 ◽  
pp. 00107
Author(s):  
Inna Korsheva ◽  
Elena Chaunina
Keyword(s):  
Broiler Chickens ◽  
Live Weight ◽  
Feed Additives ◽  
Poultry Meat ◽  
Control Group ◽  
Meat Production ◽  
Average Weight ◽  
Balanced Diet ◽  
Group Feeding ◽  
Experimental Group

The article analyses the issues of balanced feeding of broiler chickens and their balanced diet. The results of studies aimed to determine the effect of mixed feeds on the productivity of Arbor Acres cross broiler chickens and the economic efficiency of poultry meat production companies are presented. It was found that broilers from the experimental group, who were fed with improved compound feed with additional sources of proteins and carbohydrates and feed additives exceeded broilers from the control group by their live weight at all ages. At the slaughter age, the average weight was more than 39.2%. The experimental group consumed 10-15% less feed whose conversion was 2.4 and 1.9 kg in favour of the group feeding with the new compound feed. The new compound feed had a positive effect on the viability of broilers: there were no deaths during the period under study. According to the control slaughter results, it was found that the pre-slaughter live weight was higher by 35% in the experimental group. The weight of edible parts increased by 6%. The improved compound feed can be used both in industrial conditions and by poultry farms.

scholarly journals Effectiveness of a feed additive with a high degree of protein protection in feeding fresh cows

2021 ◽  
pp. 134-141
Author(s):  
E. G. Chupurina ◽  
D. A. Iurin ◽  
A. B. Vlasov ◽  
N. A. Iurina
Keyword(s):  
Feed Additive ◽  
Control Group ◽  
Group Feeding ◽  
High Level ◽  
Daily Milk Yield ◽  
High Degree ◽  
To Receive ◽  
Experimental Group ◽  
Daily Milk ◽  
Degradable Protein

Intensive dairy farming in modern conditions is impossible without a solid forage base and the use of high-quality forage resources. A high level of cow productivity presupposes adequate nutrition for the animal at all physiological periods of its life. Due to the fact that cows consume relatively little feed during the first period of lactation, the primary task of livestock breeders is to provide the body of highly productive animals with the necessary amount of energy, protein and other nutrients. The article presents the data of a scientific experiment on the use of non-degradable protein in the rumen PassPro Balance in diets for fresh cows. The feed additive under study contributes to an increase in the level of dry matter consumption by animals. The average daily milk yield of the experimental group, which received the studied supplement, significantly increased by 12.0% (P <0.05) in comparison with the control group. There was also established a tendency to an increase in the fat content in the milk of cows from the experimental group. According to the biochemical analysis of the blood serum of cows, the level of albumin in all animals was within the physiological norm. The content of globulins was below the norm in animals of all groups without significantly significant differences. The activity of the AST and ALT enzymes between the groups did not differ significantly. However, it should be noted that in both groups these indicators were within the normal range. The level of alkaline phosphatase tended to increase in the experimental group by 20.8% in comparison with the control group. Feeding PassPro Supplements Balance in the diets for the experimental group made it possible to receive 2565,00 rubles of additional profit, while the level of profitability of milk production increased by 2.02% relative to control.

scholarly journals Apelin System in Mammary Gland of Sheep Reared in Semi-Natural Pastures of the Central Apennines

Animals ◽  
2018 ◽  
Vol 8 (12) ◽  
pp. 223 ◽  
Author(s):  
Francesca Mercati ◽  
Margherita Maranesi ◽  
Cecilia Dall’Aglio ◽  
Linda Petrucci ◽  
Rolando Pasquariello ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Central Italy ◽  
Receptor Expression ◽  
Food Supplementation ◽  
System Control ◽  
Receptor System ◽  
Apelin Receptor ◽  
Experimental Group ◽  
Grassland Biodiversity ◽  
Shed Light

Sheep are the most bred species in the Central Italy Apennine using the natural pastures as a trophic resource and grazing activity is fundamental to maintain the grassland biodiversity: this goal can be reached only ensuring an economical sustainability to the farmers. This study aimed to investigate the apelin/apelin receptor system in ovine mammary gland and to evaluate the differences induced by food supplementation, in order to shed light on this system function. A flock of 15 Comisana x Appenninica adult dry ewes were free to graze from June until pasture maximum flowering (MxF). From this period to pasture maximum dryness (MxD), in addition to grazing, the experimental group (Exp) was supplemented with 600 g/day/head of cereals. Apelin and apelin receptor were assessed by Real-Time PCR and immunohistochemistry on the mammary glands of subjects pertaining to MxF, MxD and Exp groups. They were detected in alveolar and ductal epithelial cells. The pasture maximum flowering group showed significant differences in apelin expression compared with experimental and MxD groups. Apelin receptor expression significantly differed among the three groups. The reduced apelin receptor expression and immunoreactivity levels during parenchyma involution enables us to hypothesize that apelin receptor plays a modulating role in the system control.

Grass seed contamination of the wool and carcases of sheep grazing natural pasture on the north-western slopes of New South Wales

1981 ◽  
Vol 21 (111) ◽  
pp. 382 ◽  
Author(s):  
GM Lodge ◽  
BA Hamilton
Keyword(s):  
New South ◽  
Experimental Period ◽  
Sheep Grazing ◽  
Grass Seed ◽  
The North ◽  
South Wales ◽  
Natural Pasture ◽  
Hordeum Leporinum ◽  
The Mean

A group of six-month-old Merino wether weaners was grazed from January to July 1977 on a natural pasture containing seeds of wiregrass (Aristida ramosa), corkscrew grass (Stipa variabilis), barley grass (Hordeum leporinum) and burrs of Medicago minima. The sheep were then removed from the natural pasture, shorn and grazed for a further 23 months on lucerne and oats pastures that were free of grass seed. The number and species of grass seeds and burrs in the wool, hide and carcases were measured to determine the levels of seed and burr contamination and the long-term fate of seeds entering the carcase. The highest number of seeds in the wool, hide and carcase of sheep grazing natural pasture were those of wiregrass. Numbers of seeds of this species per 100 cm2 ranged from 85 to 651 in the wool, 43 to 159 in the hide, and 1 to 8 in the carcase. Most of the grass seeds in the wool were located on the stomach and shoulders, while most of the burrs were on the hind legs and stomach. Percentages of grass seed were highest about the stomach area and shoulders for the hide and carcase, respectively. After the sheep were removed from the natural pasture, and grazed on lucerne and oats pastures for 23 months, the mean grass seed contents decreased by 81% in the hide and 83% in the carcase. Wiregrass seeds were the only seeds that penetrated the hide and remained in the carcase. Numbers of contaminating seeds decreased most rapidly within 6 months of the sheep being removed from the natural pasture. Over the experimental period no seed-free hides or carcases were observed.

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