Europan Molecular Indicators of Life Investigation (EMILI) for a Future Europa Lander Mission

The Europan Molecular Indicators of Life Investigation (EMILI) is an instrument concept being developed for the Europa Lander mission currently under study. EMILI will meet and exceed the scientific and technical/resource requirements of the organic composition analyzer identified as a core instrument on the Lander. EMILI tightly couples two complementary analytical techniques, based on 1) liquid extraction and processing with capillary electrophoresis and 2) thermal and chemical extraction with gas chromatography, to robustly detect, structurally characterize, and quantify the broadest range of organics and other Europan chemicals over widely-varying concentrations. Dual processing and analysis paths enable EMILI to perform a thorough characterization of potential molecular biosignatures and contextual compounds in collected surface samples. Here we present a summary of the requirements, design, and development status of EMILI with projected scientific opportunities on the Europa Lander as well as on other potential life detection missions seeking potential molecular biosignatures in situ.

Testing Laser-Structured Antimicrobial Surfaces Under Space Conditions: The Design of the ISS Experiment BIOFILMS

Maintaining crew health and safety are essential goals for long-term human missions to space. Attaining these goals requires the development of methods and materials for sustaining the crew’s health and safety. Paramount is microbiological monitoring and contamination reduction. Microbial biofilms are of special concern, because they can cause damage to spaceflight equipment and are difficult to eliminate due to their increased resistance to antibiotics and disinfectants. The introduction of antimicrobial surfaces for medical, pharmaceutical and industrial purposes has shown a unique potential for reducing and preventing biofilm formation. This article describes the development process of ESA’s BIOFILMS experiment, that will evaluate biofilm formation on various antimicrobial surfaces under spaceflight conditions. These surfaces will be composed of different metals with and without specified surface texture modifications. Staphylococcus capitis subsp. capitis, Cupriavidus metallidurans and Acinetobacter radioresistens are biofilm forming organisms that have been chosen as model organisms. The BIOFILMS experiment will study the biofilm formation potential of these organisms in microgravity on the International Space Station on inert surfaces (stainless steel AISI 304) as well as antimicrobial active copper (Cu) based metals that have undergone specific surface modification by Ultrashort Pulsed Direct Laser Interference Patterning (USP-DLIP). Data collected in 1 x g has shown that these surface modifications enhance the antimicrobial activity of Cu based metals. In the scope of this, the interaction between the surfaces and bacteria, which is highly determined by topography and surface chemistry, will be investigated. The data generated will be indispensable for the future selection of antimicrobial materials in support of human- and robotic-associated activities in space exploration.

Micro-Bioreactors in Space: Case Study of a Yeast (Saccharomyces cerevisiae) Bioreactor With a Non-Invasive Monitoring Method

Bioreactors in space have applications from basic science to microbial factories. Monitoring bioreactors in microgravity has challenges with respect to fluidics, aeration, sensor size, sample volume and disturbance of medium and cultures. We present a case study of the development of small bioreactors and a non-invasive method to monitor dissolved oxygen, pH, and biomass of yeast cultures. Two different bioreactor configurations were tested for system volumes of 60 ml and 10.5 ml. For both configurations, the PreSens SFR vario, an optical sensor array, collected data autonomously. Oxygen and pH in the cultures were monitored using chemically doped spots, 7 mm in diameter, that were fixed to the bottom of sampling chambers. Spots emitted a fluorescent signal for DO and pH when reacted with oxygen molecules and hydrogen ions, respectively. Biomass was sensed using light reflectance at centered at 605 nm. The, optical array had three light detectors, one for each variable, that returned signals that were pre- and post-calibrated. For heterotrophic cultures requiring oxygen and respiring carbon dioxide, a hollow fiber filter, in-line with the optical array, oxygenated cells and remove carbon dioxide. This provided oxygen levels that were sufficient to maintain aerobic respiration for steady state conditions. Time series of yeast metabolism in the two bioreactors are compared and discussed. The bioreactor configurations can be easily be modified for autotrophic cultures such that carbon dioxide is enhanced and oxygen removed, which would be required for photosynthetic algal cultures.

Design and Validation of a Device for Mitigating Fluid Microgravity Effects in Biological Research in Canister Spaceflight Hardware

The major factor influencing the behavior of microbes growing in liquids in space is microgravity. We recently measured the transcriptomic response of the Gram-positive bacterium Bacillus subtilis to the microgravity environment inside the International Space Station (ISS) in spaceflight hardware called Biological Research in Canisters-Petri Dish Fixation Units (BRIC-PDFUs). In two separate experiments in the ISS, dubbed BRIC-21 and BRIC-23, we grew multiple replicates of the same B. subtilis strain in the same hardware, growth medium, and temperature with matching ground control samples (npj Micrograv. 5:1.2019, doi: 10.1038/s41526-018-0061-0). In both experiments we observed similar responses of the transcriptome to spaceflight. However, we also noted that the liquid cultures assumed a different configuration in microgravity (a toroidal shape) compared with the ground control samples (a flat disc shape), leading us to question whether the transcriptome differences we observed were a direct result of microgravity, or a secondary result of the different liquid geometries of the samples affecting, for example, oxygen availability. To mitigate the influence of microgravity on liquid geometry in BRIC canisters, we have designed an insert to replace the standard 60-mm Petri dish in BRIC-PDFU or BRIC-LED sample compartments. In this design, liquid cultures are expected to assume a more disk-like configuration regardless of gravity or its absence. We have: (i) constructed a prototype device by 3D printing; (ii) evaluated different starting materials, treatments, and coatings for their wettability (i.e., hydrophilicity) using contact angle measurements; (iii) confirmed that the device performs as designed by drop-tower testing and; (iv) performed material biocompatibility studies using liquid cultures of Bacillus subtilis and Staphylococcus aureus bacteria. Future microgravity testing of the device in the ISS is planned.

Coherent Contention Resolution Diversity Slotted ALOHA: An Improved Multiple Access Method for Satellite IoT System

For Beyond 5G/6G system, satellite communication systems become an effective component of the space and terrestrial integrated network. Among typical applications, massive Machine Type of Communication (mMTC) is a promising and challenging application. The demands of low power consumption and light signaling make random access methods as the potential solution. Up to now, contention resolution-based ALOHA methods, such as contention resolution diversity slotted ALOHA (CRDSA), improve the throughput significantly. However, its throughput will meet the inflection point soon with the normalized load increasing since collisionless packets are hardly existing. In fact, the diversity of transmitting packets is not utilized totally. In this paper, an improved random access method, named coherent CRDSA method (C-CRDSA), is proposed. It accumulates replicated packets coherently at receiver and further improve the throughput over CRDSA. Detailed derivations and simulations are given. Simulation results corroborate the effectiveness of the proposed method.

Multi-Satellite Cooperative Beamforming ALOHA for LEO Satellite IoT Networks

In this paper, we proposed a cooperative beamforming ALOHA (CBA) scheme based on linearly constrained minimum variance criterion for low Earth orbit satellite (LEO) IoT networks to solve the problem of ‘deadlock’ in multi-satellite scenario. In multi-satellite overlapping coverage areas, packets can be received by multiple satellite receivers by sending them only once, which forms the concept of spatial diversity. The cooperative beamforming collision resolution technique combined with successive interference cancellation scheme is design to efficiently resolve packet collision by iteration way at the gateway station. The performance of cooperative beamforming ALOHA scheme is evaluated via mathematical analysis and simulations. Simulation results show that the proposed CBA scheme can effectively solve the problem of ‘deadlock’ and improve the performances of random access compared with benchmark problems.

Contamination Control for Ultra-Sensitive Life-Detection Missions

A key science priority for planetary exploration is to search for signs of life in our Solar System. Life-detection mission concepts aim to assess whether or not biomolecular signatures of life are present, which requires highly sensitive instrumentation. This introduces greater risk of false positives, and perhaps false negatives. Stringent science-derived contamination requirements for achieving science measurements on life-detection missions necessitate mitigation approaches that minimize, protect from, and prevent science-relevant contamination of critical surfaces of the science payload and provide high confidence to life-detection determinations. To this end, we report on technology advances that focus on understanding contamination transfer from pre-launch processing to end of mission using high-fidelity physics in the form of computational fluid dynamics and sorption physics for monolayer adsorption/desorption, and on developing a new full-spacecraft bio-molecular barrier design that restricts contamination of the spacecraft and instruments by the launch vehicle hardware. The bio-molecular barrier isolates the spacecraft from biological, molecular, and particulate contamination from the external environment. Models were used to evaluate contamination transport for a designs reference mission that utilizes the barrier. Results of the modeling verify the efficacy of the barrier and an in-cruise decontamination activity. Overall mission contamination tracking from launch to science operations demonstrated exceptionally low probability on contamination impacting science measurements, meeting the stringent contamination requirements of femtomolar levels of compounds. These advances will enable planetary missions that aim to detect and identify signatures of life in our Solar System.

Design and Implementation of Blind Source Separation Based on BP Neural Network in Space-Based AIS

In space-based AIS (Automatic Identification System), due to the high orbit and wide coverage of the satellite, there are many self-organizing communities within the observation range of the satellite, and the signals will inevitably conflict, which reduces the probability of ship detection. In this paper, to improve system processing power and security, according to the characteristics of neural network that can efficiently find the optimal solution of a problem, proposes a method that combines the problem of blind source separation with BP neural network, using the generated suitable data set to train the neural network, thereby automatically generating a traditional blind signal separation algorithm with a more stable separation effect. At last, through the simulation results of combining the blind source separation problem with BP neural network, the performance and stability of the space-based AIS can be effectively improved.

A Hybrid Beamforming Design for Massive MIMO LEO Satellite Communications

5G and future cellular networks intend to incorporate low earth orbit (LEO) satellite communication systems (SatCom) to solve the coverage and availability problems that cannot be addressed by satellite-based or ground-based infrastructure alone. This integration of terrestrial and non terrestrial networks poses many technical challenges which need to be identified and addressed. To this aim, we design and simulate the downlink of a LEO SatCom compatible with 5G NR, with a special focus on the design of the beamforming codebook at the satellite side. The performance of this approach is evaluated for the link between a LEO satellite and a mobile terminal in the Ku band, assuming a realistic channel model and commercial antenna array designs, both at the satellite and the terminal. Simulation results provide insights on open research challenges related to analog codebook design and hybrid beamforming strategies, requirements of the antenna terminals to provide a given SNR, or required beam reconfiguration capabilities among others.