Pineapple Peel Extract as an Effective Substrate for Esterase Production from Bacillus subtilis E9

2020 ◽  
Vol 77 (10) ◽  
pp. 3024-3034
Author(s):  
Padinjarakavil Soumya ◽  
Jayachandran Kochupurackal
Keyword(s):  
Bacillus Subtilis ◽  
Pineapple Peel ◽  
Esterase Production ◽  
Peel Extract

scholarly journals UJI AKTIVITAS ANTIBAKTERI SPRAY BAU KAKI EKSTRAK KULIT JERUK NIPIS (Citrus aurantifolia) DENGAN VARIASI Gelling agent TERHADAP BAKTERI Bacillus subtilis

2021 ◽  
Vol 9 (1) ◽  
pp. 18-26
Author(s):  
Ade Maria Ulfa ◽  
◽  
Nofita Nofita ◽  
Bangun Saras Sandi ◽  
◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Bacterial Infections ◽  
Inhibition Zone ◽  
Gelling Agent ◽  
Apocrine Glands ◽  
Citrus Aurantifolia ◽  
Bacterium Bacillus Subtilis ◽  
Bacterium Bacillus ◽  
Lime Peel ◽  
Peel Extract

ABSTRACT Sweat is produced by the apocrine glands, if infected by bacteria that play a role in the decay process will certainly produce foot odor. Some of the bacteria that cause, including Staphylococcus epidermis, Corynebacterium acne and there is one bacterium that causes pungent foot odor that is Bacillus subtilis. Bacillus subtilis enzyme leucine dehydrogenase produced the highest, resulting in isovaleric acid foot odor. Lime peel (Citrus aurantifolia) has the potential to be developed for the antibacterial active ingredient of foot odor contained in tannins, alkaloids and flavonoids. Spray can be effective for inhibition of feet due to water fleas or bacterial infections. The purpose of this research is to test the inhibitory zone of the preparation of foot odor spray ethanol extract of lime peel (Citrus aurantifolia) with variations of gelling agent. Bacterial inhibition zone testing on extracts of lime peel spray preparations using the disc method. This test was carried out on spray with extract concentration of 0% extract base carbopol, 0% extract base HPMC, 0.2% extract base carbopol, 0.2% extract base HPMC, 0.4% extract base carbopol, 0.4% extract base HPMC and positive control with an average inhibition zone of 9,13 mm, 9,12 mm, 11,86 mm, 11,29 mm, 13,17 mm, 12,30 mm, 8,13 mm against the bacterium Bacillus subtilis. Antibacterial test results were analyzed using ONE WAY ANOVA, the results of statistical analysis on the preparation of lime peel extract showed a significant inhibition zone difference of 0.000 (P = <0.05) between all concentrations. Lime peel extract spray is effective in inhibiting the bacterium Bacillus subtilis. Key words: Sweat of foot odor, Lime skin (Citrus aurantifolia), Spray, bacteri Bacillus subtilis

scholarly journals Alcoholic lung injury: pineapple peel extract modulates antioxidant enzymes and attenuates lipid peroxidation in rat models

2016 ◽  
Vol 2 (1) ◽  
Author(s):  
Ochuko L. Erukainure ◽  
John A. Ajiboye ◽  
Oluwatoyin Y. Okafor ◽  
Emeka E. Okoro ◽  
Godfrey Asieba ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Enzymes ◽  
Lung Injury ◽  
Rat Models ◽  
Pineapple Peel ◽  
Peel Extract

scholarly journals Antibacterial activity of mixed pineapple peel (Ananas comosus) extract and calcium hydroxide paste against Enterococcus faecalis

2018 ◽  
Vol 51 (1) ◽  
pp. 20
Author(s):  
Intan Fajrin Arsyada ◽  
Devi Rianti ◽  
Elly Munadziroh
Keyword(s):  
Antibacterial Activity ◽  
Calcium Hydroxide ◽  
Enterococcus Faecalis ◽  
Root Canal ◽  
Antibacterial Properties ◽  
Root Canal Treatment ◽  
Ananas Comosus ◽  
Treatment Groups ◽  
Pineapple Peel ◽  
Peel Extract

Background: Enterococcus faecalis (E. faecalis) is the bacteria most commonly resulting from failed root canal treatment. Intracanal medicament is used to enhance the success of root canal treatment. A material widely used for this purpose is calcium hydroxide. However, its ineffectiveness in eliminating E. faecalis requires the addition of other antibacterial substances, such as iodoform which has the disadvantage of having toxic effects on tissues. Pineapple peel has antibacterial properties because it contains chemical compounds, such as flavonoid, saponin, tannin, as well as the enzyme bromelain. Purpose: The aim of the study was to determine the antibacterial activity of a mixture of pineapple peel extract at 6.25% and 12.5% concentrations and calcium hydroxide paste at a ratio of 1:1 compared to100% calcium hydroxide and a mixture of calcium hydroxide and iodoform paste against E. faecalis. Methods: The research was laboratory-based experiment in nature. Sample groups were divided into two control groups (one featuring100% calcium hydroxide paste and a second featuring a mixture of calcium hydroxide and iodoform paste) and two treatment groups (mixture of pineapple peel extract and calcium hydroxide paste in 6.25% and 12.5% concentrations with ratio 1:1). The method was using agar diffusion. The result data were analyzed by One Way Anova test. Results: The highest average of the inhibitory zone occurred in group with a mixture of pineapple peel extract 12.5% and calcium hydroxide paste while the smallest average was that of group with a mixture of calcium hydroxide and iodoform paste. Conclusion: Mixture of pineapple peel extract in 6.25% and 12.5% concentrations and calcium hydroxide paste ratio 1:1 has higher antibacterial activity than paste of 100% calcium hydroxide and mixture of calcium hydroxide and iodoform paste againts E. faecalis.

scholarly journals Effect of peroxide treatment on the structure and transparency of bacterial cellulose film

2018 ◽  
Vol 204 ◽  
pp. 05015 ◽  
Author(s):  
Heru Suryanto ◽  
Tito Arif Sutrisno ◽  
M. Muhajir ◽  
Neena Zakia ◽  
Uun Yanuhar
Keyword(s):  
Bacterial Cellulose ◽  
Diffraction Peak ◽  
Cellulose Film ◽  
X Ray Diffraction ◽  
X Ray ◽  
Structure And Morphology ◽  
Pineapple Peel ◽  
Diffraction Angle ◽  
Structure Observation ◽  
Peel Extract

The cellulose extracts from plants are need of energy and have potential in damaging the environment so that an alternative cellulose source with more efficient results is by using bacteria to produce the cellulose fibers. This study aims to determine the effect of hydrogen peroxide (H2O2) treatment on the structure and transparency of biopolymer bacterial cellulose film (BCF). The method used is the making of BCF by utilizing pineapple peel extract and then cooking at 80°C for 120 min. with H2O2 concentration of 0%, 2.5%, 5%, and 7,5%. Drying is done in the electric oven and then observed the structure and morphology using X-ray diffraction and transparency test, respectively. The result of structure observation shows that the intensity of the highest diffraction peak lies at a diffraction angle of 22° achieved at 5% peroxide treatment with crystallinity and crystal index of 85.1% and 82.4%, respectively. Transparency testing shows that the higher the concentration of peroxide used, the better the transparency.

Effect of Drying Methods on the Structure of Bacterial Cellulose from Pineapple Peel Extract

2020 ◽  
Vol 851 ◽  
pp. 79-85
Author(s):  
Heru Suryanto ◽  
Muhamad Muhajir ◽  
Neena Zakia ◽  
Uun Yanuhar ◽  
Aminnudin Aminnudin ◽  
...  
Keyword(s):  
High Pressure ◽  
Bacterial Cellulose ◽  
Freeze Drying ◽  
Film Structure ◽  
Drying Methods ◽  
Drying Method ◽  
Cellulose Films ◽  
Pineapple Peel ◽  
High Pressure Homogenizer ◽  
Peel Extract

Properties of Bacterial Cellulose was depended on the fermentation conditions to produce BC as well as the processing steps for modifying the Bacterial Cellulose microstructure. This study reports on the comparison effect of drying method on Bacterial Cellulose films structure produced from Pineapple Peel Extract. The drying method was done in the oven and freeze-drying. Pellicle as results of fermentation by bacteria was dried in the oven. High-pressure homogenization was applied before the freeze-drying method. BC film structure was observed using scanning electron microscopy and evaluated using X-ray diffraction. The results show that the peak of diffractogram shows crystalline peaks in a relatively similar position, which are at about 14° and 22°. High-pressure homogenizer process before freeze-drying results the structure with higher crystalline compare than oven drying. The index of crystalline and degree of crystalline of BC film in the freeze-drying method were higher than those in the oven with a value of 83% and 86% compared than 81% and 84%, respectively. Drying methods to pellicle in the oven and freeze-drying results in the degree of crystalline of 79% and 71%, respectively. The morphology of the freeze-drying methods contains a more porous structure.

scholarly journals ANTIOXIDANT POTENTIAL OF DIFFERENT PARTS OF BOGOR PINEAPPLE (ANANAS COMOSUS [L.] MERR. VAR. QUEEN) CULTIVATED IN WEST JAVA-INDONESIA

2018 ◽  
Vol 11 (1) ◽  
pp. 129
Author(s):  
Irda Fidrianny ◽  
Veliana Virna ◽  
Muhamad Insanu
Keyword(s):  
Ethyl Acetate ◽  
Antioxidant Activities ◽  
Ananas Comosus ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Antioxidant Power ◽  
Pineapple Peel ◽  
Dpph Method ◽  
Peel Extract ◽  
Different Parts

  Objective: The aims of this research were to observe antioxidant activities from different parts of Bogor pineapple (Ananas comosus [L.] Merr. Var. Queen) using two antioxidant testing methods which were 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) and correlation of total phenolic and flavonoid contents with their inhibitory concentration 50% (IC50) of DPPH and exhibitory concentration 50% (EC50) of FRAP.Methods: Each sample was extracted by reflux using different polarity solvents. Antioxidant activities were determined using DPPH and FRAP assays, total phenolic content (TPC) using Folin–Ciocalteu reagent, flavonoid content by Chang’s method, and correlation with their IC50 DPPH and EC50 FRAP were analyzed by Pearson’s method.Results: IC50 DPPH of various extracts of different parts of Bogor pineapple ranged from 0.13 to 68.17μg/ml. The ethyl acetate peel extract of Bogor pineapple presented the highest TPC (7.84 g GAE/100 g) while the highest total flavonoid content (10.84 g QE/100 g) was shown by ethyl acetate bract extract of Bogor pineapple. TPC in peel extract of Bogor pineapple had negative and significant correlation with their EC50 FRAP. The IC50 DPPH and EC50 FRAP of peel extract of Bogor pineapple showed positive and significant correlation.Conclusion: All different part extracts of Bogor pineapple (except n-hexane flesh extract, peel extract, and bract extract) were categorized as a very strong antioxidant by DPPH method. Phenolic compounds in peel extract of Bogor pineapple were the major contributor in antioxidant activities by FRAP method. DPPH and FRAP methods gave linear results in antioxidant activities of Bogor pineapple peel extract.

scholarly journals DAYA ANTI BAKTERI EKSTRAK KULIT NANAS (Ananas comosus) TERHADAP PERTUMBUHAN BAKTERI Enterococcus faecalis ANTIBACTERIAL POTENCY OF PINEAPPLE PEEL EXTRACT (Ananas comosus) ON Enterococcus faecalis GROWTH

2016 ◽  
Vol 6 (2) ◽  
pp. 61
Author(s):  
Rega Maurischa Arantika Putri ◽  
Tamara Yuanita ◽  
Mohammad Roelianto
Keyword(s):  
Enterococcus Faecalis ◽  
Root Canal ◽  
Positive Control ◽  
Ananas Comosus ◽  
Control Group ◽  
Control Group Design ◽  
Antibacterial Drugs ◽  
Bacteria Growth ◽  
Pineapple Peel ◽  
Peel Extract

Background: Root canal infections is caused by the colonization of microorganisms. Microorganisms that commonly found in root canal failure is Enterococcus faecalis. These bacteria can be found in 80-90% of root canal infections. Currently, many plants are used as antibacterial drugs. Pineapple is one plant that can be used as antibacterial drugs. Pineapple peel has a variety of antibacterial chemical compounds, such as, bromelain, saponins, tannins and flavonoids that can inhibit the growth of Enterococcus faecalis bacteria. Aim: To obtain concentration of antibacterial from pineapple peel extract on Enterococcus faecalis growth. Methods: This study was an experimental laboratory with Post Test Only control group design using Enterococcus faecalis ATCC 29212 bacteria that were diluted based on Mc. Farland standard 1.5 x 108 CFU / ml with pineapple peel extract treatment concentration of 100%, 50%, 25%, 12.5%, 6.25%, 3.125%, 1.56%, 0, 78% and then planted in nutrient agar media surface evenly. Results: At concentration of 3.125% pineapple peel extract showed that the growth of colonies was less than 90% of positive control and concentration of 6.25% pineapple peel extract had no visible Enterococcus faecalis bacteria growth as much as 99.9%. Conclusion: The pineapple peel extract (Ananas comosus) have antibacterial power on the growth of Enterococcus faecalis bacterial colonies with Minimal Inhibitory Concentration (MIC) of 3.125% and the Minimum Bactericidal Concentration (MBC) of 6.25%.

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