Functional analysis reveals pleiotropic effects of rice RING-H2 finger protein gene OsBIRF1 on regulation of growth and defense responses against abiotic and biotic stresses

2008 ◽  
Vol 68 (1-2) ◽  
pp. 17-30 ◽  
Author(s):  
Huizhi Liu ◽  
Huijuan Zhang ◽  
Yayun Yang ◽  
Guojun Li ◽  
Yuxia Yang ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Protein Gene ◽  
Defense Responses ◽  
Pleiotropic Effects ◽  
Abiotic And Biotic Stresses ◽  
Biotic Stresses ◽  
Finger Protein ◽  
Regulation Of Growth

scholarly journals Molecular and Functional Analysis of U-box E3 Ubiquitin Ligase Gene Family in Rice (Oryza sativa)

2021 ◽  
Vol 22 (21) ◽  
pp. 12088
Author(s):  
Me-Sun Kim ◽  
Kwon-Kyoo Kang ◽  
Yong-Gu Cho
Keyword(s):  
Functional Analysis ◽  
Drought Stress ◽  
Gene Family ◽  
Ubiquitin Ligase ◽  
Expression Patterns ◽  
E3 Ubiquitin Ligase ◽  
Sequencing Analysis ◽  
Abiotic And Biotic Stresses ◽  
Cis Acting ◽  
Biotic Stresses

Proteins encoded by U-box type ubiquitin ligase (PUB) genes in rice are known to play an important role in plant responses to abiotic and biotic stresses. Functional analysis has revealed a detailed molecular mechanism involving PUB proteins in relation to abiotic and biotic stresses. In this study, characteristics of 77 OsPUB genes in rice were identified. Systematic and comprehensive analyses of the OsPUB gene family were then performed, including analysis of conserved domains, phylogenetic relationships, gene structure, chromosome location, cis-acting elements, and expression patterns. Through transcriptome analysis, we confirmed that 16 OsPUB genes show similar expression patterns in drought stress and blast infection response pathways. Numerous cis-acting elements were found in promoter sequences of 16 OsPUB genes, indicating that the OsPUB genes might be involved in complex regulatory networks to control hormones, stress responses, and cellular development. We performed qRT-PCR on 16 OsPUB genes under drought stress and blast infection to further identify the reliability of transcriptome and cis-element analysis data. It was confirmed that the expression pattern was similar to RNA-sequencing analysis results. The transcription of OsPUB under various stress conditions indicates that the PUB gene might have various functions in the responses of rice to abiotic and biotic stresses. Taken together, these results indicate that the genome-wide analysis of OsPUB genes can provide a solid basis for the functional analysis of U-box E3 ubiquitin ligase genes. The molecular information of the U-box E3 ubiquitin ligase gene family in rice, including gene expression patterns and cis-acting regulatory elements, could be useful for future crop breeding programs by genome editing.

scholarly journals Lectin Receptor-Like Kinases: The Sensor and Mediator at the Plant Cell Surface

2020 ◽  
Vol 11 ◽  
Author(s):  
Yali Sun ◽  
Zhenzhen Qiao ◽  
Wellington Muchero ◽  
Jin-Gui Chen
Keyword(s):  
Cell Surface ◽  
Plant Cell ◽  
Environmental Changes ◽  
Abiotic And Biotic Stresses ◽  
Biotic Stresses ◽  
Lectin Receptor ◽  
Protection Systems ◽  
The Status ◽  
Signaling Components ◽  
Regulation Of Growth

Lectin receptor-like kinases (LecRLKs), a plant-specific receptor-like kinase (RLK) sub-family, have been recently found to play crucial roles in plant development and responses to abiotic and biotic stresses. In this review, we first describe the classification and structures of Lectin RLKs. Then we focus on the analysis of functions of LecRLKs in various biological processes and discuss the status of LecRLKs from the ligands they recognize, substrate they target, signaling pathways they are involved in, to the overall regulation of growth-defense tradeoffs. LecRLKs and the signaling components they interact with constitute recognition and protection systems at the plant cell surface contributing to the detection of environmental changes monitoring plant fitness.

scholarly journals SlWRKY45 Interacts with Jasmonate-ZIM Domain Proteins to Negatively Regulate Defense Against Root Knot Nematode Meloidogyne incognita in Tomato

2022 ◽  
Author(s):  
Huang Huang ◽  
Wenchao Zhao ◽  
Hui Qiao ◽  
Chonghua Li ◽  
Xuechun Ma ◽  
...  
Keyword(s):  
Meloidogyne Incognita ◽  
Defense Responses ◽  
Wrky Transcription Factor ◽  
Parasitic Nematodes ◽  
Allene Oxide ◽  
Wild Type ◽  
Root Knot Nematode ◽  
Abiotic And Biotic Stresses ◽  
Biotic Stresses ◽  
Gall Index

Root knot nematode (RKN), a kind of plant parasitic nematodes, leads to large reduction of crop yield, and seriously damages the agricultural production. The phytohormone jasmonates (JAs) act as important signals to regulate resistance against multiple abiotic and biotic stresses. However, little is known about the mechanism of JA-mediated defense responses against RKN in tomato. In this study, we found that the WRKY transcription factor SlWRKY45 interacts with most of the Jasmonate-ZIM domain proteins (JAZs) in yeast and plant. Overexpression of SlWRKY45 decreased plant resistance to RKN Meloidogyne incognita with increased gall index. We further generated slwrky45 mutants using the CRISPR/Cas9 technology, and discovered that the gall index and the number of nematodes and females in slwrky45 mutants are significantly reduced compared with wild type, as inoculated with RKN Meloidogyne incognita. Moreover, the contents of jasmonic acid and JA-isoleucine (JA-Ile) were highly increased in slwrky45 mutants with RKN Meloidogyne incognita infection compared with wild type. Furthermore, EMSA, and Dual-LUC assays demonstrated that SlWRKY45 directly binds and represses jasmonate biosynthesis gene ALLENE OXIDE CYCLASE ( AOC). Overall, our findings reveled that JAZ-interaction protein SlWRKY45 negatively controls plant defense against RKN Meloidogyne incognita by the regulation of JA biosynthesis in tomato.

Cloning and functional analysis of SCTF-1 encoding a C2H2-type zinc finger protein from soybean

2012 ◽  
Vol 34 (6) ◽  
pp. 749-756 ◽  
Author(s):  
Bing SONG ◽  
Pi-Wu WANG ◽  
Yong-Ping FU ◽  
Xu-Hong FAN ◽  
Hai-Feng XIA ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Zinc Finger ◽  
Zinc Finger Protein ◽  
Finger Protein

Identification of Plant Protein Kinases in Response to Abiotic and Biotic Stresses Using SuperSAGE

2011 ◽  
Vol 12 (7) ◽  
pp. 643-656 ◽  
Author(s):  
Ederson Akio Kido ◽  
Pedranne Kelle de Araujo Barbosa ◽  
Jose Ribamar Costa Ferreira Neto ◽  
Valesca Pandolfi ◽  
Laureen Michelle Houllou-Kido ◽  
...  
Keyword(s):  
Protein Kinases ◽  
Plant Protein ◽  
Abiotic And Biotic Stresses ◽  
Biotic Stresses

scholarly journals Proteomics and Metabolomics Studies on the Biotic Stress Responses of Rice: an Update

Rice ◽  
2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Kieu Thi Xuan Vo ◽  
Md Mizanor Rahman ◽  
Md Mustafizur Rahman ◽  
Kieu Thi Thuy Trinh ◽  
Sun Tae Kim ◽  
...  
Keyword(s):  
Stress Responses ◽  
Biotic Stress ◽  
Defense Mechanisms ◽  
Defense Responses ◽  
Global Changes ◽  
Biotic Stresses ◽  
Novel Proteins ◽  
Stress Agent ◽  
Global Food ◽  
Shed Light

AbstractBiotic stresses represent a serious threat to rice production to meet global food demand and thus pose a major challenge for scientists, who need to understand the intricate defense mechanisms. Proteomics and metabolomics studies have found global changes in proteins and metabolites during defense responses of rice exposed to biotic stressors, and also reported the production of specific secondary metabolites (SMs) in some cultivars that may vary depending on the type of biotic stress and the time at which the stress is imposed. The most common changes were seen in photosynthesis which is modified differently by rice plants to conserve energy, disrupt food supply for biotic stress agent, and initiate defense mechanisms or by biotic stressors to facilitate invasion and acquire nutrients, depending on their feeding style. Studies also provide evidence for the correlation between reactive oxygen species (ROS) and photorespiration and photosynthesis which can broaden our understanding on the balance of ROS production and scavenging in rice-pathogen interaction. Variation in the generation of phytohormones is also a key response exploited by rice and pathogens for their own benefit. Proteomics and metabolomics studies in resistant and susceptible rice cultivars upon pathogen attack have helped to identify the proteins and metabolites related to specific defense mechanisms, where choosing of an appropriate method to identify characterized or novel proteins and metabolites is essential, considering the outcomes of host-pathogen interactions. Despites the limitation in identifying the whole repertoire of responsive metabolites, some studies have shed light on functions of resistant-specific SMs. Lastly, we illustrate the potent metabolites responsible for resistance to different biotic stressors to provide valuable targets for further investigation and application.

scholarly journals Core RNA Interference Genes Involved in miRNA and Ta-siRNA Biogenesis in Hops and Their Expression Analysis after Challenging with Verticillium nonalfalfae

2021 ◽  
Vol 22 (8) ◽  
pp. 4224
Author(s):  
Urban Kunej ◽  
Jernej Jakše ◽  
Sebastjan Radišek ◽  
Nataša Štajner
Keyword(s):  
Rna Interference ◽  
Expression Analysis ◽  
Cannabis Sativa ◽  
Defense Responses ◽  
Resistant Cultivar ◽  
Post Inoculation ◽  
Biotic Stresses ◽  
Argonaute 2 ◽  
Expression Of Genes ◽  
Verticillium Nonalfalfae

RNA interference is an evolutionary conserved mechanism by which organisms regulate the expression of genes in a sequence-specific manner to modulate defense responses against various abiotic or biotic stresses. Hops are grown for their use in brewing and, in recent years, for the pharmaceutical industry. Hop production is threatened by many phytopathogens, of which Verticillium, the causal agent of Verticillium wilt, is a major contributor to yield losses. In the present study, we performed identification, characterization, phylogenetic, and expression analyses of three Argonaute, two Dicer-like, and two RNA-dependent RNA polymerase genes in the susceptible hop cultivar Celeia and the resistant cultivar Wye Target after infection with Verticillium nonalfalfae. Phylogeny results showed clustering of hop RNAi proteins with their orthologues from the closely related species Cannabis sativa, Morus notabilis and Ziziphus jujuba which form a common cluster with species of the Rosaceae family. Expression analysis revealed downregulation of argonaute 2 in both cultivars on the third day post-inoculation, which may result in reduced AGO2-siRNA-mediated posttranscriptional gene silencing. Both cultivars may also repress ta-siRNA biogenesis at different dpi, as we observed downregulation of argonaute 7 in the susceptible cultivar on day 1 and downregulation of RDR6 in the resistant cultivar on day 3 after inoculation.

scholarly journals Recent Development on Plant Aldehyde Dehydrogenase Enzymes and Their Functions in Plant Development and Stress Signaling

Genes ◽  
2020 ◽  
Vol 12 (1) ◽  
pp. 51
Author(s):  
Adesola J. Tola ◽  
Amal Jaballi ◽  
Hugo Germain ◽  
Tagnon D. Missihoun
Keyword(s):  
Plant Development ◽  
Critical Analysis ◽  
Current Knowledge ◽  
Stress Signaling ◽  
Oxygen Species ◽  
Abiotic And Biotic Stresses ◽  
Aldehyde Dehydrogenases ◽  
Biotic Stresses ◽  
Wide Range ◽  
Excessive Accumulation

Abiotic and biotic stresses induce the formation of reactive oxygen species (ROS), which subsequently causes the excessive accumulation of aldehydes in cells. Stress-derived aldehydes are commonly designated as reactive electrophile species (RES) as a result of the presence of an electrophilic α, β-unsaturated carbonyl group. Aldehyde dehydrogenases (ALDHs) are NAD(P)+-dependent enzymes that metabolize a wide range of endogenous and exogenous aliphatic and aromatic aldehyde molecules by oxidizing them to their corresponding carboxylic acids. The ALDH enzymes are found in nearly all organisms, and plants contain fourteen ALDH protein families. In this review, we performed a critical analysis of the research reports over the last decade on plant ALDHs. Newly discovered roles for these enzymes in metabolism, signaling and development have been highlighted and discussed. We concluded with suggestions for future investigations to exploit the potential of these enzymes in biotechnology and to improve our current knowledge about these enzymes in gene signaling and plant development.

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