Determination of In Vitro Antidiabetic Effects, Antioxidant Activities and Phenol Contents of Some Herbal Teas

2008 ◽  
Vol 63 (1) ◽  
pp. 27-33 ◽  
Author(s):  
Aynur Büyükbalci ◽  
Sedef Nehir El
Keyword(s):  
Antioxidant Activities ◽  
Antidiabetic Effects

scholarly journals Leaf Extracts of Aerva lanata Inhibit the Activities of Type 2 Diabetes-Related Enzymes and Possess Antioxidant Properties

2018 ◽  
Vol 2018 ◽  
pp. 1-7 ◽  
Author(s):  
Musbau Adewunmi Akanji ◽  
Samson Olasunkanmi Olukolu ◽  
Mutiu Idowu Kazeem
Keyword(s):  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Leaf Extracts ◽  
Chemically Induced ◽  
Radical Scavenging Ability ◽  
Aerva Lanata ◽  
Radical Scavenging Properties

The leaves of Aerva lanata are one of the indigenous medicinal plants used in the management of diabetes mellitus and its associated complications in Africa. However, its effect on the activities of diabetes-related enzymes has not been investigated. This study evaluated the in vitro inhibitory effects of different extracts of the A. lanata leaf on the activities of diabetes-related enzymes (α-amylase and α-glucosidase) and chemically induced free radicals. Aqueous, ethanol, and hydroethanol extracts of A. lanata leaves were subjected to a standard enzyme inhibition assay followed by determination of modes of inhibition of the enzymes. The antioxidant activities of the extracts were evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS). The results obtained showed that the hydroethanol extract of the A. lanata leaf optimally inhibited both α-amylase (IC50: 2.42 mg/mL) and α-glucosidase (IC50: 0.23 mg/mL). The Lineweaver-Burk plot revealed that the mode of inhibition of both enzymes by the hydroethanol extract was uncompetitive. However, the hydroethanol and aqueous extracts displayed the best DPPH and ABTS radical-scavenging ability, respectively. It can be concluded that the A. lanata extract inhibited the activities of both α-amylase and α-glucosidase uncompetitively, which may be attributed to its free radical-scavenging properties and rich phenolic composition.

scholarly journals CHEMICAL CONSTITUENTS, IN VITRO ANTIOXIDANT ACTIVITY, ORAL ACUTE TOXICITY AND LD50 DETERMINATION OF MORINGA OLEIFERA LEAVES

2017 ◽  
Vol 9 (5) ◽  
pp. 240
Author(s):  
Manal Mortady Hamed ◽  
Aboelfetoh Mohamed Abdalla ◽  
Mosad Ahmed Ghareeb ◽  
Said Abdelhalim Saleh
Keyword(s):  
Antioxidant Activity ◽  
Acute Toxicity ◽  
Antioxidant Activities ◽  
Total Phenolic ◽  
Dry Extract ◽  
Phenolic Contents ◽  
Moringa Oleifera Leaves ◽  
Oral Acute Toxicity

Objective: The objective of this study was undertaken to estimate the total phenolic contents (TPCs), in vitro antioxidant of different solvent extracts of M. oleifera leaves, oral acute toxicity and LD50 determination of the 85% methanolic extract as well as the chromatographic isolation and identification of the extract constituents.Methods: The antioxidant activity of different solvent extracts of Moringa oleifera leaves were estimated using three antioxidant assays and the total phenolic contents (TPCs) were also evaluated using Folin-Ciocalteu’s assay. The n-BuOH extract undergoes further chromatographic isolation owing to the high antioxidant activity using 2, 2'-diphenyl-1-picrylhydrazyl radical (DPPH) method, which resulted in the isolation of seven compounds.Results: The results showed that the TPCs values of the tested extracts were varied from 309.52 to 43.28 mg gallic acid equivalent/g dry extract. The reducing power antioxidant activities (RPAA) were 0.434, 0.402, 0.395, 0.149, 0.143 and 0.124, while the total antioxidant capacity (TAC) values were 316.43, 203.35, 181.56, 86.70, 76.62 and 50.83 mg ascorbic acid equivalent/g dry extract; for n-BuOH, EtOAc, 85% MeOH, H2O, CH2Cl2, and pet. ether extracts, respectively. The oral acute toxicity study of the 85% methanol extracts of M. oleifera and M. peregrina revealed that; their LD50 values were 3458.3 and 4125 mg/kg respectively, thus the two plants could be classified as slightly toxic in the scale of Hodge and Sterner which reflected their nutrient values as edible plants. The isolated compounds were identified on the basis of their 1H and 13C-NMR spectra as; cis-p-coumaric acid 4-O-(2'-O-β-D-apiofuranosyl)-β-D-glucopyranoside (1), chlorogenic acid (2), niazirin (3), 3,4-dihydroxy-β-phenylethoxy-O-α-L-rhamnopyranosyl-(l→2)-α-L-rhamnopyranosyl-(1→3)-4-O-caffeoyl-β-D-glucopyranoside (4), gallic acid (5), taxifolin (6), and benzyl-carbamo-thioethionate (7).Conclusion: The M. oleifera leaves showed promising antioxidant activities and slightly toxic behavior.

scholarly journals Determination of in vitro antidiabetic effects of Zingiber officinale Roscoe

2012 ◽  
Vol 48 (4) ◽  
pp. 601-607 ◽  
Author(s):  
Naila Abdul Sattar ◽  
Fatma Hussain ◽  
Tahira Iqbal ◽  
Munir Ahmad Sheikh
Keyword(s):  
Therapeutic Potential ◽  
Zingiber Officinale ◽  
Postprandial Glucose ◽  
Protein Glycation ◽  
Zingiber Officinale Roscoe ◽  
In Vitro Effects ◽  
Dose Dependent ◽  
Antidiabetic Effects

Aqueous extracts of Zingiber officinale rhizomes were studied to evaluate their antidiabetic effects on protein glycation and on the diffusion of glucose in vitro in the present study. Zingiber officinale rhizome aqueous extract were examined at concentrations of 5, 10, 20 and 40 g/L. The antidiabetic effects were found to be dose-dependent. Antidiabetic potential of Zingiber officinale was mainly through inhibition of the glucose diffusion and to a limited extent by reducing the glycation. However, further studies are needed to determine in vitro effects of therapeutic potential by restraining postprandial glucose absorptions and plasma protein glycations in diabetic subjects.

scholarly journals In Vitro Antimicrobial and Antioxidant Activities of Lactobacillus coryniformis BCH-4 Bioactive Compounds and Determination of their Bioprotective Effects on Nutritional Components of Maize (Zea mays L.)

Molecules ◽  
2020 ◽  
Vol 25 (20) ◽  
pp. 4685
Author(s):  
Mahwish Salman ◽  
Anam Tariq ◽  
Anam Ijaz ◽  
Shazia Naheed ◽  
Abeer Hashem ◽  
...  
Keyword(s):  
Aspergillus Flavus ◽  
Antioxidant Activities ◽  
Fungal Growth ◽  
Good Alternative ◽  
Proximate Analysis ◽  
Nutritional Components ◽  
Lactobacillus Coryniformis ◽  
Β Carotene

Lactic acid bacteria (LAB) can synthesize antimicrobial compounds (AMCs) with nutritional and bioprotective properties in crops and food products. In the current study, AMCs of Lactobacillus coryniformis BCH-4 were evaluated to control fungal spoilage in maize grains. On maize grains treated with 75%–100% (v/v) concentrated AMCs, no fungal growth was observed even after 72 h of Aspergillus flavus inoculation. Proximate analysis of treatments A1 (raw grains), A2 (A. flavus inoculated grains) and A3 (A. flavus + AMCs inoculated grains) revealed that moisture was significantly (p ≤ 0.05) high in A2 than A3 and A1. Meanwhile, protein, fat, fiber and ash contents were significantly decreased in A2 compared to A1 and A3. Moreover, β-carotene contents were not statistically different between A1 and A3, while in A2 it was significantly decreased. HPLC analysis revealed the presence of 2-oxopropanoic acid, 2-hydroxypropane-1,2,3-tricarboxylic acid, 2-hydroxybutanedioic acid, 2-hydroxypropanoic acid, propanedioic acid and butanedioic acid, which also showed antifungal activity against Aspergillus flavus. FTIR spectroscopy revealed the presence of hydroxyl, carbonyl and ester-groups along with organic and fatty acids, thereby indicating their participation in inhibitory action. Furthermore, the AMCs were found to be a good alternative to chemical preservatives, thereby not only preserving the nutritive qualities but increasing the shelf life as well.

scholarly journals Anticholinergic, Antidiabetic and Antioxidant Activities of Ferula orientalis L. Determination of Its Polyphenol Contents by LC-HRMS

2021 ◽  
Vol 15 (6) ◽  
pp. 513-528
Author(s):  
Hatice Kızıltaş ◽  
Zeynebe Bingöl ◽  
Ahmet C. Gören ◽  
Saleh H. Alwasel ◽  
İlhami Gülçin
Keyword(s):  
Metal Ion ◽  
Antioxidant Activities ◽  
Water Extract ◽  
Ethanolic Extract ◽  
Scavenging Activity ◽  
Ic50 Values ◽  
Dpph Scavenging Activity ◽  
Dpph Scavenging

To evaluate the antioxidant activity of evaporated ethanolic extract of Ferula orientalis L. (EEFO) and lyophilized water extract of Ferula orientalis L. (WEFO) several in vitro antioxidant methods such as ABTS•+ scavenging activity, DPPH· scavenging activity, Fe3+reduction method, cupric ions (Cu2+) reduction capacity, and metal ion (Fe2+)-binding activities using ferrozine reagent were separately performed. Also, BHT, α-tocopherol and ascorbic acid were used as the standard antioxidant molecules. Moreover, some phenolic compounds that are responsible for antioxidant abilities of EEFO and WEFO were determined by LC-HRMS. EEFO and WEFO demonstrated effective antioxidant abilities when compared with the standards. EEFO demonstrated IC50 values of 1.946 µg/mL against acetylcholinesterase (AChE), 0.815 µg/mL against α-glycosidase, and 0.675 µg/mL against α-amylase.

scholarly journals Studies on the Determination of Antioxidant Activity and Phenolic Content of Plant Products in India (2000–2017)

2019 ◽  
Vol 102 (5) ◽  
pp. 1407-1413
Author(s):  
Ramesh K Sharma ◽  
Maria Micali ◽  
Alessandra Pellerito ◽  
Anna Santangelo ◽  
Sofia Natalello ◽  
...  
Keyword(s):  
Food Intake ◽  
Antioxidant Activities ◽  
Antioxidant Compounds ◽  
Spectroscopic Techniques ◽  
Herbal Extracts ◽  
Plant Products ◽  
Food Intake Regulation ◽  
Intake Regulation

Abstract Background: Ayurveda, the traditional Indian therapeutic system, involves herbs and spices as drug ingredients and a kind of food intake regulation. The health and curative aspects of plant products are often ascribed to their antimicrobial and antioxidant activities (AA). However, it seems somewhat possible to correlate the AA of herbal extracts or plant isolates with their phenolic contents (PC). Objective: Indian researchers have carried out some tests for the determination of AA and PC of plant products that are worth mentioning. Methods: Among the herbal analysis techniques, the important contributions of Indians and people of Indian origin in this field include the remarkable separation of phenolic compounds, including the use of reversed-phase columns, on-line HPLC, the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay to effectively screen and identify antioxidant compounds from herbal extracts, and other chromatographic, spectrographic, and spectroscopic techniques, in the analysis of complex biological matrices. Results: The aim of this paper is to present an overview of such arguments with reference to herbal drugs and food intake regulation for specific ailments. Conclusions: The lack of uniformity in the use of test protocols for antioxidant assays (particularly using the DPPH free radical) and differences in in vitro mechanisms of antiradical activity and in vivo metabolism of polyphenols have been the issues associated with these experiments that have been raised by researchers in the period from 2000 onward.

Chemical properties, antioxidant activities and in vitro fermentation profiles of some shrubs of North Western Tunisia

2018 ◽  
Author(s):  
H. Selmi ◽  
M. Hasnaoui ◽  
A. Bahri ◽  
C. Abbes ◽  
S. Dallali ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Antioxidant Activities ◽  
Chemical Properties ◽  
Gas Production ◽  
Total Phenolic ◽  
Myrtus Communis ◽  
In Vitro Fermentation ◽  
In Vitro Gas Production

The main aims of this study were to analyze the chemical composition and total phenolic, and to evaluate the in vitro fermentation and antioxidant activities from some shrubs (Erica arborea, Myrtus communis, Arbutus unedo and Phillyrea latifolia). The determination of the chemical composition, total phenolic, flavonoid contents and the antioxidant activity were investigated by using in vitro methods. The in vitro gas production and ruminal parameters were determined in the presence of goat rumen juice. Total nitrogen content was significantly high with Myrtus communis (p less than 0.01). Total phenolic and flavonoid content vary by species and season (p less than 0.01). It can be concluded that all parameters were significantly affected by the seasonal period and the shrubs nature. Expect the OM that was similar regardless the seasonal period.

scholarly journals Antidiabetic Micro-/Nanoaggregates from Ge-Gen-Qin-Lian-Tang Decoction Increase Absorption of Baicalin and Cellular Antioxidant Activity In Vitro

2017 ◽  
Vol 2017 ◽  
pp. 1-8 ◽  
Author(s):  
Dai Lin ◽  
Qian Du ◽  
Huiqin Wang ◽  
Guanzhen Gao ◽  
Jianwu Zhou ◽  
...  
Keyword(s):  
Antioxidant Activities ◽  
Pharmacological Study ◽  
Cell Monolayer ◽  
Redox Status ◽  
Diabetic Rats ◽  
Cellular Viability ◽  
Active Components ◽  
Cellular Protection ◽  
Antidiabetic Effects

The antidiabetic effects of Ge-Gen-Qin-Lian-Tang decoction (GQD) have been proven clinically. In a pharmacological study conducted on STZ-induced diabetic rats, the constitutive aggregates/sediments of Ge-Gen-Qin-Lian-Tang decoction exhibited stronger hypoglycemic and antioxidant activities compared to the soluble compositions. This study aims to demonstrate the pharmacological properties of aggregates derived from GQD by measuring permeability of the active monomer phytochemicals (e.g., baicalin) in a Caco-2 cell monolayer and determine the cellular viability, intracellular redox status (MDA and SOD), and insulin secretion of pancreatic β-cell line, INS-1, following STZ-induced oxidative stress. The aggregates were separated into three fractions, namely, “MA (microaggregates),” “400 g supernatant,” and “MNA (micro-/nanoaggregates),” by centrifugation at 400 ×g and 15000 ×g, respectively. Aggregates in the sediment increased baicalin absorption, showed little toxicity to β-cells, elevated intracellular SOD levels, and significantly suppressed oxidative damage effects on cellular viability and functions. The “MA” fraction had a larger particle size and provided higher antioxidant cellular protection than “MNA” in vitro, implying that the sediments may be the active components in the herbal decoction. The actions of these micro-/nanoaggregates may provide a new perspective for understanding the antidiabetic effects of herbal decoctions and aid in interpretation of synergistic actions between the multiple components.

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