Visible light photocatalytic antibacterial activity of Ni-doped and N-doped TiO2 on Staphylococcus aureus and Escherichia coli bacteria

2015 ◽  
Vol 23 (5) ◽  
pp. 4111-4119 ◽  
Author(s):  
Jirapat Ananpattarachai ◽  
Yuphada Boonto ◽  
Puangrat Kajitvichyanukul
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Visible Light ◽  
Doped Tio2 ◽  
Escherichia Coli Bacteria ◽  
Visible Light Photocatalytic

scholarly journals UJI AKTIVITAS ANTIBAKTERI DAUN JERUK KALAMANSI (Citrofortunella microcarpa) TERHADAP BAKTERI Staphylococcus aureus DAN Escherichia coli

Alotrop ◽  
2019 ◽  
Vol 3 (2) ◽  
Author(s):  
Deza Oktasila ◽  
Nurhamidah Nurhamidah ◽  
Dewi Handayani
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Essential Oil ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Raw Material ◽  
Diffusion Method ◽  
Citrus Leaves ◽  
Escherichia Coli Bacteria

This study aims to examine the antibacterial activity of ethanol extract and essential oil of Kalamansi citrus leaves (Citrofortunella microcarpa) against Staphylococcus aureus and Escherichia coli bacteria. The raw material of Kalamansi citrus leaves is obtained from the Village of Pondok Kubang, Bengkulu Tengah (3.70060S, 102.35780E). Ethanol extract from Kalamansi citrus leaves was obtained by maceration using ethanol 96%, then ethanol extract was made dilution concentration 40 ; 20; , 10; and 5%. The essential oil of Kalamansi citrus leaves is obtained by water-vapor distillation, then made variations of concentration 20;, 15;, 10;  and 5%. The method used to test the antibacterial activity is the paper disc diffusion method , the antibacterial activity is shown by the diameter of the inhibiting  zone formed. The data of antibacterial test result were analyzed by using One Way Anova test which showed the effect of treatment on the growth of test bacteria seen from the value (P <0,01) and continued by Duncan test to know the effect of the treatment. The results showed that ethanol extract of Kalamansi citrus leaves had antibacterial activity against S. aureus and E. coli bacteria with moderate inhibitory diameter is  7.20 and 5.73 mm at concentration 40%, while antibacterial activity on essential oil of Kalamansi citrus leaves is categorized as strong with inhibition zone diameter is 14.83 and 13.00 mm at concentration 20%.

scholarly journals The Antibacterial Activity Of Several Sponges From The Waters Of Tasik Ria Against Escherichia coli And Staphylococcus aureus bacteria

2018 ◽  
Vol 7 (1) ◽  
pp. 1
Author(s):  
Abrianto A. O. Rompis ◽  
Fitje Losung ◽  
Deiske A. Sumilat ◽  
Agung B. Windarto ◽  
Stenly Wullur ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Natural Compounds ◽  
E Coli ◽  
Escherichia Coli Bacteria ◽  
And Staphylococcus Aureus ◽  
Positive Results ◽  
Sponge Extract

The sponge is one of the sea organisms that has a prospect as a source of natural compounds including peptides, steroids, asetogenin, terpenoids, alkaloids, cyclic halide and nitrogen. This research was directed to obtain several species of sponges from the waters of Tasik Ria as well as testing the antibacterial activity of extracts from some of the sponge against the bacteria Escherichia coli and Staphylococcus aureus. From the identification, seven species of sponges were found, which consists of: Amphimedon sp., Axinosa sp., Aaptos sp., Theonella sp., Cribochalina sp., Hyrtios sp., and Lendenfeldia sp. The tests of antibacterial activity of the extracts from these sponges against test bacteria E. coli and S. aureus showed some positive results. Extract from Axinosa sp. sponge(16 mm) showed the strongest antibacterial activity on Escherichia coli bacteria. Followed by Hyrtios sp. extract (13.5 mm), Aaptos sp. extract (13 mm), Lendenfeldia sp. extract (13 mm) and Cribochalinai sp. extract(10.5 mm). While the the tests on Staphylococcus aureus bacteria showed that the strongest antibacterial activity was found from Axinosa sp. sponge extract (16.5 mm), followed by the extract from Aaptos sp. (15 mm), Lendenfeldia sp. extract (14.5 mm), Hyrtios sp. extract(13.5 mm) and Cribochalina sp. extract (11 mm).Keywords: Sponge, antibacterial, Escherichia coli, Staphylococcus aureus ABSTRAK Spons merupakan salah satu biota laut yang sangat prospektif sebagai sumber senyawa bahan-bahan alami antara lain peptide, terpenoid, steroid, asetogenin, alkaloid, halide siklik dan senyawa nitrogen. Penelitian ini diarahkan untuk mendapatkan beberapa spesies spons dari perairan Tasik Ria serta menguji aktivitas antibakteri dari beberapa ekstrak spons terhadap bakteri Escherichia coli dan bakteri Staphylococcus aureus. Hasil identifikasi spons ditemukan sebanyak tujuh spesies yang terdiri dari: Amphimedon sp., Axinosa sp., Aaptos sp., Theonella sp., Hyrtios sp., Cribochalina sp. dan Lendenfeldia sp.. Aktivitas antibakteri dari beberapa ekstrak spons terhadap bakteri uji E. coli dan S. aureus terdapat diameter zona hambat bervariasi yaitu bakteri Escherichia coli menunjukkan aktivitas antibakteri ekstrak spons terkuat pada spons Axinosa sp (16 mm), disusul ekstrak spons Hyrtios sp. (13,5 mm), ekstrak spons Aaptos sp. (13 mm), ekstrak spons Lendenfeldia sp. (13 mm) dan ekstrak spons Cribochalinai sp. (10,5 mm).  Sedangkan pada bakteri Staphylococcus aureus menunjukkan aktivitas antibakteri ekstrak spons terkuat yaitu:  ekstrak spons Axinosa sp. (16,5 mm), disusul ekstrak spons Aaptos sp. (15 mm), ekstrak spons Lendenfeldia sp. (14,5 mm), ekstrak spons Hyrtios sp. (13,5 mm) dan ekstrak spons Cribochalina sp.(11mm).Kata Kunci : Spons, Antibakteri, Escherichia coli, Staphylococcus aureus

scholarly journals Potential Bacterial Symbion of Sea Urchin As a Multi-Drug Resistant (MDR) Antibacterial Agent Against Staphylococcus aureus and Escherichia Coli Bacteria

2018 ◽  
Vol 23 (3) ◽  
pp. 131
Author(s):  
Suzana Kristy Satriani Fofied ◽  
Agus Sabdono ◽  
Diah Permata Wijayanti
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Sea Urchin ◽  
Pathogenic Bacteria ◽  
Bacterial Resistance ◽  
Symbiotic Bacteria ◽  
Dilution Method ◽  
Drug Resistant ◽  
Escherichia Coli Bacteria

Staphylococcus aureus and Escherichia coli are pathogenic bacteria agent of many human diseases. Those bacteria infect in various levels and also been antibiotic resistants. Bacterial resistance has become a serious global problem. The purposes of this study were to isolate and identify the symbiotic bacteria of the Sea Urchin that have an antibacterial activity of the Strain Multi-Drug Resistant (MDR) against Staphylococcus aureus and Escherichia coli. Sea Urchin was taken from Panjang island, Jepara Indonesia at 2-3 m depth. The symbiotic bacteria were isolated from Sea Urchin by using dilution method and spread plate method. Phenotypic characteristics was observed on colony shape, color and texture of growing bacteria. While the streak method was used to purify bacterial symbion. The antibacterial activity test was performed using overlay method. The results showed that 3 out of 37 isolates have antibacterial activity against Staphylococcus aureus and Escherichia coli. The BB.03.35 isolate was selected for molecular identification due to the largest inhibitory zone diameter. The sequence of this bacterium showed 97% homology and closely related to Pseudoalteromonas flavipulchra.

scholarly journals ISOLASI DAN UJI AKTIVITAS ANTIBAKTERI DARI BAKTERI YANG BERASOSIASI DENGAN ALGA Turbinaria ornata (Turner) J. Agardh SERTA IDENTIFIKASI SECARA BIOKIMIA

PHARMACON ◽  
2019 ◽  
Vol 8 (2) ◽  
pp. 351
Author(s):  
Cicilia Kosasi ◽  
Widya A. Lolo ◽  
Sri Sudewi
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Diffusion Method ◽  
Genus Bacillus ◽  
Biochemical Tests ◽  
Isolation And Purification ◽  
Turbinaria Ornata ◽  
Gram Staining ◽  
Escherichia Coli Bacteria

ABSTRACT Algae are a place to live for various microorganisms’ symbiosis with them, and some of them were known to be used as antibacterial. This study aims to determine the antibacterial activity of associated bacteria isolates from Turbinaria ornata algae obtained from the Bay of Manado against Staphylococcus aureus and Escherichia coli bacteria. In the course of testing the isolation and purification of symbiotic bacteria from Turbinaria ornata algae, then antibacterial activity was testing using agar diffusion method, then gram staining and biochemical tests were carried out including motility test,  tets, carbohydrate fermentation test, citrate test, lysine test, indole test and catalase test. The result showed  that there were 3 bacterial isolates, which had antibacterial activity in medium category against S.aureus and E.coli bacteria. Based on the results of biochemical identification, the isolates that have the greatest inhibitory power, they were thought to belong to genus Bacillus and the genus Yersinia.Keywords : Antibacterial, Symbionic bacteria, Turbinaria ornata (Turner) J.   Agardh. ABSTRAK Alga merupakan tempat hidup berbagai mikroorganisme yang bersimbiosis dengannya, dan beberapa dari spesies alga diketahui sering dimanfaatkan salah satunya  sebagai antibakteri. Penelitian ini bertujuan untuk mengetahui adanya aktivitas antibakteri dari isolat bakteri yang berasosiasi dengan alga Turbinaria ornata yang diperoleh dari Teluk Manado terhadap bakteri  Staphylococcus aureus dan Escherichia coli. Dalam pengujian dilakukan isolasi dan purfifikasi bakteri simbion dari alga Turbinari ornata, kemudian dilakukan pengujian aktivitas antibakteri menggunakan metode difusi agar, selanjutnya silakukan pewarnaan gram dan uji biokimia meliputi uji motilitas, uji H2S, uji fermentasi karbohidrat, uji sitrat, uji lisin, uji indol dan uji katalase. Hasil penelitian menunjukkan bahwa terdapat 3 isolat bakteri yang mempunyai daya aktivitas antibakteri dalam kategori sedang terhadap bakteri S. aureus dan E. coli. Berdasarkan hasil identifikasi secara biokimia isolat yang mempunyai daya hambat paling besar diduga termasuk dalam genus Bacillus dan genus Yersinia. Kata kunci : Antibakteri, Bakteri simbion, Turbinaria ornata (Turner) J. Agardh.

scholarly journals Fitokimia Dan Aktivitas Antibakteri Dari Daun KAF(Chisocheton sp. (C. DC) HARMS)

Jurnal MIPA ◽  
2019 ◽  
Vol 8 (2) ◽  
pp. 42
Author(s):  
Florentin Natalia Melsadalam ◽  
Dewa Gede Katja ◽  
Meiske S. Sangi
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Secondary Metabolites ◽  
Ethyl Acetate ◽  
Leaf Extract ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Inhibitory Zone ◽  
Escherichia Coli Bacteria

Telah dilakukan penelitian yang bertujuan untuk mengidentifikasi senyawa metabolit sekunder  dari ekstrak daun kaf menggunakan tiga jenis pelarut yakni n-heksan, etil asetat, dan metanol. Ekstrak yang diperoleh diuji aktivitas antibakterinya dengan metode difusi yang menggunakan bakteri Staphylococcusaureus dan Escherichia coli. Dari hasil pengujian fitokimia, ketiga ekstrak tersebut menunjukan bahwa sampel mengandung senyawa; alkaloid, saponin, steroid, flavonoid dan tanin. Pengujian aktivitas antibakteri  dengan metode difusi menunjukan hasil yang paling tinggi aktivitas antibakteri adalah  estrak etil asetat dengan zona hambat 35,75 mm terhadap bakteri  Staphylococcus aureus, sedangkan pada ekstrak metanol memiliki zona hambat 23,75 mm terhadap bakteri Escherichia coliResearch has been carried out aimed at identifying secondary metabolites of kaf leaf extract using three types of solvents, namely n-hexane, ethyl acetate, and methanol.The obtained extracts were tested for antibacterial activity by diffusion method using Staphylococcus aureus and Escherichia coli bacteria. From the results of phytochemical testing, the three extracts showed that the sample contained compounds; alkaloids, saponins, steroids, flavonoids and tannins. Antibacterial activity testing with diffusion method showed the highest antibacterial activity was estracethyl ethyl acetate with 35.75 mm inhibition zone against Staphylococcus aureus bacteria, whereas methanol had 23.75 mm inhibitory zone against Escherichia coli bacteria

scholarly journals UJI AKTIVITAS ANTIBAKTERI EKSTRAK DAN FRAKSI ASCIDIAN (Lissoclinum badium) DARI PERAIRAN PULAU MANTEHAGE

PHARMACON ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 897
Author(s):  
Falinry Woran ◽  
Defny S. Wewengkang ◽  
Meilani Jayanti
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Bioactive Compounds ◽  
Methanol Fraction ◽  
Agar Method ◽  
Ethanol Extracts ◽  
Escherichia Coli Bacteria ◽  
And Staphylococcus Aureus ◽  
Growth Of Bacteria

ABSTRACTLissoclinum badium is a type of ascidian that contains bioactive compounds. This study aims to determine of presence of antibacterial activity from extracts and fractions of Lissoclinum badium collected from Mantehage Island Manado against Escherichia coli and Staphylococcus aureus bacteria. Samples were extracted by maceration method using 95% ethanol solvent and fractionated using solvents of chloroform, n-hexane and methanol. Antibacterial activity was carried out by the disk diffusion agar method. The results showed that the ethanol extracts an methanol fraction had activity to inhibit the growth of Escherichia coli bacteria with strong category. Meanwhile, against the Staphylococcus aureus the ethanol extracts, chloroform and n-hexane fractions had ability to inhibit the growth of bacteria with weak category.. Keywords: Antibacterial activity, Lissoclinum badium, Escherichia coli, Staphylococcus aureus.  ABSTRAKLissoclinum badium merupakan salah satu jenis tunikata yang memiliki kandungan senyawa bioaktif yang dapat digunakan untuk pengobatan berbagai penyakit. Penelitian ini bertujuan untuk mengetahui adanya aktivitas antibakteri dari ekstrak dan fraksi  Lissoclinum badium yang diperoleh dari Pulau Mantehage Manado terhadap Escherichia coli dan Staphylococcus aureus. Sampel diekstraksi dengan metode maserasi menggunakan pelarut etanol 95% dan fraksinasi menggunakan pelarut metanol, kloroform, dan n-heksan. Aktivitas antibakteri dilakukan dengan  metode difusi agar cakram kertas. Hasil penelitian menunjukkan bahwa ekstrak etanol dan fraksi metanol memiliki aktivitas untuk menghambat bakteri Escherichia coli dan Staphylococcus aureus dengan daya hambat kuat. Sedangkan untuk fraksi kloroform dan fraksi n-heksan memiliki aktivitas untuk menghambat bakteri Staphylococcus aureus saja dengan daya hambat sedang. Kata kunci: Aktivitas antibakteri, Lissoclinum badium, Escherichia coli, Staphylococcus aureus.

scholarly journals PENENTUAN AKTIVITAS EKSTRAK ETANOL CABAI MERAH DALAM MENGHAMBAT PERTUMBUHAN BAKTERI

2021 ◽  
Vol 26 (1) ◽  
pp. 64-73
Author(s):  
Alfi Sapitri ◽  
Eva Diansari Marbun ◽  
Ulfayani Mayasari
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Ethanol Extract ◽  
Clear Zone ◽  
Capsicum Annum ◽  
Activity Test ◽  
Mueller Hinton ◽  
Mueller Hinton Agar ◽  
Escherichia Coli Bacteria

Penelitian ini bertujuan untuk melakukan uji fitokimia terhadap ekstrak etanol Cabai merah (Capsicum annum L.) dan uji aktivitasnya terhadap pertumbuhan bakteri Staphylococcus aureus dan Escherichia coli. Pengujian aktivitas antibakteri ini menggunakan metode difusi agar dengan menggunakan kertas cakram dengan tiga kali pengulangan. Ekstrak etanol cabai merah dibuat dengan konsentrasi 45, 60, 75, dan 90%. Uji aktivitas antibakteri dilakukan dengan metode Kirby Bauer. Media yang digunakan sebagai uji antibakteri pada penelitian ini yaitu Mueller-Hinton Agar (MHA). Hasil skrining fitokimia terhadap ekstrak etanol cabai merah menunjukkan adanya senyawa metabolit sekunder. Senyawa metabolit sekunder tersebut yaitu alkaloid, flavonoid, tanin, saponin, dan Steroid/triterpenoid. Steroid berfungsi sebagai antimikroba. Hasil penelitian ini juga mengungkapkan adanya kandungan senyawa capsaicin yang terkenal sebagai senyawa aktif antimikroba. Ekstrak etanol cabai merah memiliki aktivitas antibakteri terhadap bakteri Staphylococcus aureus dan Escherichia coli zona bening terendah pada konsentrasi 45% dan zona bening tertinggi pada konsentrasi 90%. Dapat disimpulkan bahwa ekstrak cabai merah memiliki aktivitas antibakteri terhadap penghambatan pertumbuhan bakteri.THE ABILITY OF RED CHILI ETHANOL EXTRACT IN INHIBITHING THE GROWTH OF BACTERIAThis study aimed to perform a phytochemical test on the ethanolic extract of red chili (Capsicum annum L.) and test its activity against the growth of Staphylococcus aureus and Escherichia coli bacteria. This antibacterial activity test used the agar diffusion method using disc paper with three repetitions. Red chili ethanol extract was made with concentrations of 45%, 60%, 75% and 90%. The antibacterial activity test was carried out using the Kirby Bauer method. The medium used as an antibacterial test in this study was Mueller-Hinton Agar (MHA). The results of phytochemical screening of red chili ethanol extract showed the presence of secondary metabolites. The secondary metabolites are alkaloids, flavonoids, tannins, saponins, and steroids/triterpenoids. Steroids serve as  antimicrobials. The results of this study also revealed the presence of capsaicin compounds which are known as active antimicrobial compounds. Red chili ethanol extract had antibacterial activity against Staphylococcus aureus and Escherichia coli bacteria in the lowest clear zone at a concentration of 45% and the highest clear zone at a concentration of 90%. It can be concluded that red chili extract has antibacterial activity against bacterial growth inhibition.

UJI AKTIVITAS ANTIBAKTERI DARI EKSTRAK ETANOL DAUN PERIA LAUT (Colubrina Asiatica L.) TERHADAP BAKTERI STAPHYLOCOCCUS AUREUS DAN ESCHERICHIA COLI

2019 ◽  
Vol 2 (1) ◽  
pp. 14-18
Author(s):  
Firdaus Fahdi ◽  
Harwitavia Harwitavia ◽  
Herviani Sari
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Leaf Extract ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Positive Control ◽  
Negative Control ◽  
Antibiotic Drugs ◽  
Escherichia Coli Bacteria

The discovery of new antibiotic drugs is getting more and more reactive. The plant of the peria laut leaf is one of the drugs that is often used as a traditional medicine and contains bioactive compounds of polyphenols, flavonoids, and saponins, which can inhibit antibacterial growth. Purpose of this study was to determine the antibacterial activity of ethanol extract of peria laut leaves (Colubrina asiatica L.) against Staphylococcus aureus and Escherichia coli bacteria. Method the experimental, of the sample used was concentrated marine peria laut leaf extract of 25mg/ml, 50mg/ml, 75mg/ml, 100mg/ml, 200mg/ml, 300mg/ml, 400mg/ml, and 500mg/ml, positive control of amoxicillin tablet 500 mg, negative control of dimethylsulfoxide with the method of disc diffusion testing using media Nutrient Agar. Results the showed thet the peria laut leaf extract positively contained bioactive alkaloid compounds, flavonoids, saponins, steroids, and tannins, and had inhibitory effects on Staphylococcus aureus and Escgerichia coli bacteria with various concentrations which had been tested on the average area of the highest inhibition zone of 18,6 mm in Staphylococcus aureus bacteria, and 10,2 mm in Escherichia coli bacteria. Conclusion peria laut leaf extract (Colubrina asiatica L.) has antibacterial activity against Staphylococcus aureus and Escherichia coli bacteria in the most inhibitory zone at a concentration of 500mg/ml with a diameter of 18,6 in Staphylococcus aureus bacteria and 10,2 in Escherichia coli.

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