Exogenous ABA induces drought tolerance in upland rice: the role of chloroplast and ABA biosynthesis-related gene expression on photosystem II during PEG stress

2014 ◽  
Vol 36 (8) ◽  
pp. 2219-2227 ◽  
Author(s):  
Kaiqiong Teng ◽  
Junzhou Li ◽  
Lei Liu ◽  
Yingchun Han ◽  
Yanxiu Du ◽  
...  
Keyword(s):  
Gene Expression ◽  
Photosystem Ii ◽  
Drought Tolerance ◽  
Upland Rice ◽  
Related Gene ◽  
Exogenous Aba ◽  
Aba Biosynthesis ◽  
Peg Stress

The Effects of Abscisic Acid on Photosynthesis in Tomato Under Sub-High Temperature and High Light Stress

2016 ◽  
Vol 13 (10) ◽  
pp. 7189-7198
Author(s):  
Shuang Gang ◽  
Yufeng Liu ◽  
Tao Lu ◽  
Mingfang Qi ◽  
Xiaoxi Guan ◽  
...  
Keyword(s):  
Abscisic Acid ◽  
High Temperature ◽  
Light Stress ◽  
High Light ◽  
Exogenous Aba ◽  
Photosystems I And Ii ◽  
Genes Expression ◽  
Irreversible Reduction ◽  
Aba Biosynthesis

The present study investigated the role of abscisic acid (ABA) application in photosynthesis, photosystems I and II (PSI and PSII), antioxidant system and ABA-related genes expression under sub-high temperature and high light (STHL) stress. STHL treatment led to an irreversible reduction in the photosynthetic rate (Pn), damaged PSII firstly at three hours, and then inhibited RuBPCase activity at seven hours, at last injured PSI after eleven hours. During 11 hours STHL stress, exogenous ABA can alleviate the degree of Pn decreasing, improve the activity of RuBPCase, protect PSII to photoinhibition, and promote the ability of reactive oxygen removal. When severe stress occured, exogenous ABA has certain effect, but can not ease photoinhibition and photodamage. In addition, exogenous ABA effected significantly on genes of upstream regulatory ABA biosynthesis key enzymes and downstream response MYB transcription factors.

scholarly journals Development and validation of an individualized immune-related gene pairs prognostic signature in papillary renal cell carcinoma

2020 ◽  
Author(s):  
Xianghong Zhou ◽  
Shi Qiu ◽  
Di Jin ◽  
Kun Jin ◽  
Xiaonan Zheng ◽  
...  
Keyword(s):  
Gene Expression ◽  
High Risk ◽  
Risk Group ◽  
High Risk Group ◽  
Related Gene ◽  
Prognostic Signature ◽  
Gene Pairs ◽  
Immune Related Gene ◽  
Immune Related Genes

Abstract Background: Papillary renal carcinoma (PRCC) is one of the important subtypes of kidney cancer, with a high degree of heterogeneity. At present, there is still a lack of robust and accurate biomarkers for the diagnosis, prognosis and treatment selection of PRCC. Considering the important role of tumor immunity in PRCC, we aim to construct a signature based on immune-related gene pairs (IRGPs) to estimate the prognostic of patients with PRCC.Methods: We obtained gene expression profiling and clinical information of patients with PRCC from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO), which were divided into discovery and validation cohorts, respectively. The immune-related genes in the samples were used to construct gene pairs, and the immune-related genes pairs (IRGPs) with robust impact for overall survival (OS) were screened out to construct the signature by univariate analysis, multivariate Cox analysis, and least absolute shrinkage and selection operator (Lasso) analysis. Then we verified the prognostic role of the signature, and assessed the relationship between this signature with tumor immune infiltration and functional pathways.Results: A total of 315 patients were included in our study, and divided to discovery (n=287) and validation (n=28) cohorts. Finally, we selected 14 IRGPs with a panel of 22 unique genes to construct the prognostic signature. According to the signature, we stratified patients into high-risk group and low-risk group. In both discovery and validation cohorts, the results of Kaplan-Meier analysis showed that there were significant differences in OS between the two groups (p<0.001). Combined with multiple clinical pathological factors, the results of multivariate analyses confirmed that this signature was an independent predictor of OS (HR, 3.548; 95%CI, 2.096−6.006; p<0.001). The results of immune infiltration analysis demonstrated that the abundance of multiple tumor-infiltration lymphocytes such as CD8+ T cells, Tregs, and T follicular cell helper were significantly higher in the high-risk group. Functional analysis showed that multiple immune-related signaling pathways were enriched in the high-risk group.Conclusions: We successfully established an individualized prognostic immune-related gene pairs signature, which can accurately and independently predict the OS of patients with PRCC.

Biological Function of Changes in RNA Metabolism in Plant Adaptation to Abiotic Stress

2019 ◽  
Vol 60 (9) ◽  
pp. 1897-1905 ◽  
Author(s):  
Akihiro Matsui ◽  
Kentaro Nakaminami ◽  
Motoaki Seki
Keyword(s):  
Gene Expression ◽  
Abiotic Stress ◽  
Metabolic Regulation ◽  
Rna Stability ◽  
Rna Metabolism ◽  
Stress Adaptation ◽  
Transcriptional Level ◽  
Related Gene ◽  
Structural Modifications

Abstract Plant growth and productivity are greatly impacted by environmental stresses. Therefore, plants have evolved various sophisticated mechanisms for adaptation to nonoptimal environments. Recent studies using RNA metabolism-related mutants have revealed that RNA processing, RNA decay and RNA stability play an important role in regulating gene expression at a post-transcriptional level in response to abiotic stresses. Studies indicate that RNA metabolism is a unified network, and modification of stress adaptation-related transcripts at multiple steps of RNA metabolism is necessary to control abiotic stress-related gene expression. Recent studies have also demonstrated the important role of noncoding RNAs (ncRNAs) in regulating abiotic stress-related gene expression and revealed their involvement in various biological functions through their regulation of DNA methylation, DNA structural modifications, histone modifications and RNA–RNA interactions. ncRNAs regulate mRNA transcription and their synthesis is affected by mRNA processing and degradation. In the present review, recent findings pertaining to the role of the metabolic regulation of mRNAs and ncRNAs in abiotic stress adaptation are summarized and discussed.

scholarly journals Angiogenesis related gene expression significantly associated with the prognostic role of an urothelial bladder carcinoma

2020 ◽  
Vol 9 (5) ◽  
pp. 2200-2210
Author(s):  
Jianfeng Wang ◽  
Meng Guo ◽  
Xiaofeng Zhou ◽  
Zhenshan Ding ◽  
Xing Chen ◽  
...  
Keyword(s):  
Gene Expression ◽  
Bladder Carcinoma ◽  
Related Gene ◽  
Prognostic Role ◽  
Urothelial Bladder Carcinoma ◽  
Urothelial Bladder ◽  
Angiogenesis Related Gene

Exogenous ABA induces salt tolerance in indica rice (Oryza sativa L.): The role of OsP5CS1 and OsP5CR gene expression during salt stress

2013 ◽  
Vol 86 ◽  
pp. 94-105 ◽  
Author(s):  
Siriporn Sripinyowanich ◽  
Pongsathorn Klomsakul ◽  
Bongkoj Boonburapong ◽  
Thapana Bangyeekhun ◽  
Tadao Asami ◽  
...  
Keyword(s):  
Gene Expression ◽  
Oryza Sativa ◽  
Salt Stress ◽  
Salt Tolerance ◽  
Oryza Sativa L ◽  
Indica Rice ◽  
Exogenous Aba

scholarly journals Histone crotonylation-centric gene regulation

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Kun Li ◽  
Ziqiang Wang
Keyword(s):  
Gene Expression ◽  
Gene Regulation ◽  
Gene Transcription ◽  
Target Genes ◽  
Regulatory Elements ◽  
Related Gene ◽  
Post Translational Modification ◽  
Histone Lysine ◽  
Dna Regulatory Elements

AbstractHistone crotonylation is a recently described post-translational modification that occurs at multiple identified histone lysine crotonylation sites. An increasing number of studies have demonstrated that histone crotonylation at DNA regulatory elements plays an important role in the activation of gene transcription. However, among others, we have shown that elevated cellular crotonylation levels result in the inhibition of endocytosis-related gene expression and pro-growth gene expression, implicating the complexity of histone crotonylation in gene regulation. Therefore, it is important to understand how histone crotonylation is regulated and how it, in turn, regulates the expression of its target genes. In this review, we summarize the regulatory factors that control histone crotonylation and discuss the role of different histone crotonylation sites in regulating gene expression, while providing novel insights into the central role of histone crotonylation in gene regulation.

scholarly journals Role of Inflammatory Related Gene Expression in Clear Cell Renal Cell Carcinoma Development and Clinical Outcomes

2011 ◽  
Vol 186 (5) ◽  
pp. 2071-2077 ◽  
Author(s):  
Weiqi Tan ◽  
Michelle A.T. Hildebrandt ◽  
Xia Pu ◽  
Maosheng Huang ◽  
Jie Lin ◽  
...  
Keyword(s):  
Gene Expression ◽  
Renal Cell Carcinoma ◽  
Cell Carcinoma ◽  
Clinical Outcomes ◽  
Renal Cell ◽  
Clear Cell ◽  
Related Gene ◽  
Cell Renal Cell Carcinoma

scholarly journals POS0398 ADIPONECTIN INDUCES SYNOVIAL ANGIOGENESIS IN RHEUMATOID ARTHRITIS THROUGH METABOLIC REMODELING

2021 ◽  
Vol 80 (Suppl 1) ◽  
pp. 428.2-428
Author(s):  
L. Wang ◽  
W. Tan ◽  
F. Wang ◽  
M. Zhang
Keyword(s):  
Rheumatoid Arthritis ◽  
Gene Expression ◽  
Endothelial Cell ◽  
Bone Erosion ◽  
Aortic Ring ◽  
Tube Formation ◽  
Related Gene ◽  
Metabolic Remodeling ◽  
Angiogenesis Related Gene

Background:Our team have previously reported that Adiponectin correlates well with synovial inflammation and progressive bone erosion in rheumatoid arthritis (RA). Angiogenesis is another important part, which plays a critical role in the pathogenesis of RA.Objectives:We hypothesized that adiponectin induces synovial angiogenesis in RA.Methods:Single-cell RNA sequencing (scRNA-Seq) was used to screen cellular changes in local knee joint of collagen-induced arthritis (CIA) after intraarticularly injected of adiponectin. Chimera models of synovium-cartilage-NOD/SCID mice, matrigel plug assay and rat aortic ring assay were performed to demonstrate the pro-angiogenesis role of adiponectin. Cellular experiment, including proliferation, migration, apoptosis, tube formation and angiogenesis related gene expression profile, were detected with Human Umbilical Vein Endothelial Cells (HUVEC) and Mice Lung Microvessel Endothelial Cell (MLMEC) after adiponectin stimulation. Seahorse was performed to clear the influence of adiponectin to cell metabolism.Results:The synovium and pannus hyperplasia worse in CIA model after intraarticularly injected of adiponectin, along with more serious synovitis and bone erosion. ScRNA-Seq of synovial tissues separated from CIA reminded that endothelial cell barbarically grows via metabolic remodeling after stimulated with adiponectin. Synovial chimera, matrigel plug and rat aortic ring shows adiponectin accelerates angiogenesis significantly in different background conditions. In vitro, endothelial cell proliferation detecting by RCTA and CCK8, migration by wound healing and transwell, apoptosis by FACS, tube formation and angiogenesis related gene expression profile by PCR-ARRAY were promoted by adiponectin in both HUVEC and MLMEC. Seahorse showed HUVEC made more use of glycolysis after co-cultured with adiponectin, a method of cell energy supply that tumor cells possess called warburg effect, that drives endothelial cell hyperplasia in severe environment.Conclusion:As a classic metabolic regulator, adiponectin exacerbates CIA by promoting angiogenesis through metabolic remodeling. The findings not only provide a novel insight into the pathogenic role of adiponectin, but also reveals a potential therapeutical strategy to attenuate revascularization in RA.Disclosure of Interests:None declared

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