Studies on the protective effects of betaine against oxidative damage during experimentally induced restraint stress in Wistar albino rats

This study is an attempt to evaluate the hepatoprotective activity ofTabernaemontana divaricataagainst DEN and Fe NTA induced liver necrosis in rats. Ethanolic extract of the whole plant ofTabernaemontana divaricataat doses of 200 and 400 mg/kg body weight and 5-fluorouracil (standard drug) was orally administered to male Wistar Albino rats once daily for 24 weeks, simultaneously treated with the carcinogen DEN and Fe NTA. In simultaneously treated animals, the plant extract significantly decreased the levels of uric acid, bilirubin, AST, ALT, and ALP in serum and increased the levels of liver marker enzymes in liver. Treatment with the extracts resulted in a significant increase in the levels of antioxidants accompanied by a marked reduction in the levels of malondialdehyde when compared to DEN and Fe NTA treated group. When compared with 200 mg/kg bw rats, 400 mg/kg bw rats and 5-fluorouracil treated rats showed better results in all the parameters. The histopathological studies confirmed the protective effects of extract against DEN and Fe NTA induced liver necrosis. Thus, it could be concluded that the use ofTabernaemontana divaricataextract in the treatment of carcinogen induced hepatic necrosis.

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Protective effects of rosmarinic acid on sepsis-induced DNA damage in the liver of Wistar albino rats

Frontiers in Genetics ◽

10.3389/conf.fgene.2015.01.00020 ◽

2015 ◽

Vol 6 ◽

Author(s):

Goktas Hatice ◽

Bacanli Merve ◽

Aydin Sevtap ◽

Taner Gokce ◽

Sahin Tolga ◽

...

Keyword(s):

Dna Damage ◽

Rosmarinic Acid ◽

Albino Rats ◽

Protective Effects ◽

Wistar Albino Rats

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Protective effects of erdosteine on rotenone-induced oxidant injury in liver tissue

Toxicology and Industrial Health ◽

10.1191/0748233704th208oa ◽

2004 ◽

Vol 20 (6-10) ◽

pp. 141-147 ◽

Cited By ~ 20

Author(s):

Alpaslan Terzi ◽

Mustafa Iraz ◽

Semsettin Sahin ◽

Atilla Ilhan ◽

Nuri Idiz ◽

...

Keyword(s):

Lipid Peroxidation ◽

Liver Tissue ◽

Antioxidant Properties ◽

Albino Rats ◽

Protective Agent ◽

Protective Effects ◽

Oxidant Injury ◽

Sod Activity ◽

Significant Difference ◽

Wistar Albino Rats

Rotenone, an insecticide of botanical origin, causes toxicity through inhibition of complex I of the respiratory chain in mitochondria. This study was undertaken to determine whether rotenone-induced liver oxidant injury is prevented by erdosteine, a mucolytic agent showing antioxidant properties. There were four groups of Male Wistar Albino rats: group one was untreated as control; the other groups were treated with erdosteine (50 mg/kg per day, orally), rotenone (2.5 mg/mL once and 1 mL/kg per day for 60 days, i.p.) or rotenone plus erdosteine, respectively. Rotenone treatment without erdosteine increased xanthine oxidase (XO) enzyme activity and also increased lipid peroxidation in liver tissue P < 0.05). The rats treated with rotenone plus erdosteine produced a significant decrease in lipid peroxidation and XO activities in comparison with rotenone group PB / 0.05). Erdosteine treatment with rotenone led to an increase in catalase (CAT) and superoxide dismutase (SOD) activities in comparison with the rotenone group PB / 0.05). There was no significant difference in nitric oxide (NO) level between groups. There were negative correlations between CAT activity and malondialdehyde (MDA) level (r= -0.934, P <0.05) with between CAT and SOD activities (r= -0.714, P <0.05), and a positive correlation between SOD activity and MDA level (r= 0.828, P <0.05) in rotenone group. In the rotenone plus erdosteine group, there was a negative correlation between XO activity and NO level in liver tissue (r= -0.833, P -0.05). In the light of these findings, erdosteine may be a protective agent for rotenone-induced liver oxidative injury in rats.

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Evaluation of Hematological, Serum Biochemical and Tissue Antioxidant Changes in Experimentally Induced Hyperlipidemia in Wistar Albino Rats

International Journal of Livestock Research ◽

10.5455/ijlr.20170512080116 ◽

2018 ◽

pp. 1

Author(s):

Hamsavath Naik ◽

Ch Srilatha ◽

K Sujatha ◽

B Sreedevi ◽

T Prasad

Keyword(s):

Albino Rats ◽

Serum Biochemical ◽

Wistar Albino Rats ◽

Experimentally Induced

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Does rosmarinic acid treatment have protective role against sepsis-induced oxidative damage in Wistar Albino rats?

Human & Experimental Toxicology ◽

10.1177/0960327115607971 ◽

2016 ◽

Vol 35 (8) ◽

pp. 877-886 ◽

Cited By ~ 21

Author(s):

M Bacanlı ◽

S Aydın ◽

G Taner ◽

HG Göktaş ◽

T Şahin ◽

...

Keyword(s):

Dna Damage ◽

Oxidative Damage ◽

Rosmarinic Acid ◽

Biological Activities ◽

Albino Rats ◽

Repair Capacity ◽

Tnf Α ◽

Liver And Kidney ◽

Wistar Albino Rats ◽

Α Level

Reactive oxygen species are believed to be involved in the development of sepsis. Plant-derived phenolic compounds are thought to be possible therapeutic agents against sepsis because of their antioxidant properties. Rosmarinic acid (RA) is a phenolic compound commonly found in various plants, which has many biological activities including antioxidant activity. The aim of this study was to investigate the effects of RA on sepsis-induced DNA damage in the lymphocytes and liver and kidney cells of Wistar albino rats by alkaline comet assay with and without formamidopyrimidine DNA glycosylase protein. The oxidative stress parameters such as superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities and total glutathione (GSH) and malondialdehyde (MDA) levels in the liver and kidney tissues and an inflammatory cytokine, tumor necrosis factor α (TNF-α) level in plasma were also evaluated. It is found that DNA damage in the lymphocytes, livers, and kidneys of the RA-treated rats was significantly lower than that in the sepsis-induced rats. RA treatment also decreased the MDA levels and increased the GSH levels and SOD and GSH-Px activities in the livers and kidneys of the sepsis-induced rats. Plasma TNF-α level was found to be decreased in the RA-treated rats. It seems that RA might have a role in the attenuation of sepsis-induced oxidative damage not only by decreasing the DNA damage but also by increasing the antioxidant status and DNA repair capacity of the animals.

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Effect of Fluoxetine on the Hippocampus of Wistar Albino Rats in Cold Restraint Stress Model

JOURNAL FOR CLINICAL AND DIAGNOSTIC RESEARCH ◽

10.7860/jcdr/2017/26958.9953 ◽

2017 ◽

Cited By ~ 1

Author(s):

Saikarthik Jayakumar

Keyword(s):

Restraint Stress ◽

Albino Rats ◽

Stress Model ◽

Cold Restraint Stress ◽

Wistar Albino Rats ◽

Cold Restraint

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Protective effects of tempol in an experimental ovarian ischemia–reperfusion injury model in female Wistar albino rats

Canadian Journal of Physiology and Pharmacology ◽

10.1139/cjpp-2016-0309 ◽

2017 ◽

Vol 95 (7) ◽

pp. 861-865 ◽

Cited By ~ 9

Author(s):

Neslihan Pınar ◽

Oya Soylu Karapınar ◽

Oğuzhan Özcan ◽

Esin Atik Doğan ◽

Suphi Bayraktar

Keyword(s):

Sham Group ◽

Ischemia Reperfusion Injury ◽

Ischemia Reperfusion ◽

Stress Index ◽

Albino Rats ◽

Protective Effects ◽

Group V ◽

Group Iii ◽

Group I ◽

Wistar Albino Rats

The aim of this study was to investigate the antioxidant effects of tempol on ovarian ischemia–reperfusion (I/R) injury in rats. Forty female Wistar albino rats were randomly divided into 5 groups: Group I, sham; Group II, ischemia (I); Group III, I/R; Group IV, I/R + tempol 30 mg/kg i.p; Group V, I/R + tempol 50 mg/kg i.p. Oxidative stress index (OSI) was significantly higher in the ischemia group and the I/R group than in the sham group. Catalase levels were significantly lower in the I/R group than in the I/R + tempol 30 mg/kg i.p. and the I/R + tempol 50 mg/kg i.p. groups. Glutathione peroxidase levels were lower in the I/R group than in the I/R + tempol 30 mg/kg i.p. and the I/R + tempol 50 mg/kg i.p. groups. MDA levels were significantly lower in the I/R + tempol 30 mg/kg i.p. group and the I/R + tempol 50 mg/kg i.p. group than in the I/R group. The levels of the histopathological parameters were significantly decreased in the I/R + tempol 50 mg/kg i.p. group compared with the I/R group. Tempol can be used for reducing ovarian I/R injury.

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Antihyperlipidemic and Biochemical Activities of Mcy Protein in Streptozotocin Induced Diabetic Rats

Cellular Physiology and Biochemistry ◽

10.1159/000373954 ◽

2015 ◽

Vol 35 (4) ◽

pp. 1326-1334 ◽

Cited By ~ 7

Author(s):

Saritha Marella ◽

Dilip Rajasekhar Maddirela ◽

Kameswara Rao Badri ◽

Malaka Venkateshwarulu Jyothi Kumar ◽

Apparao Chippada

Keyword(s):

Blood Glucose ◽

Fasting Blood Glucose ◽

Diabetic Rats ◽

Albino Rats ◽

Protective Effects ◽

Tissue Lipid ◽

Lipid Levels ◽

Liver And Kidney ◽

Streptozotocin Induced Diabetes ◽

Wistar Albino Rats

Background: This study was aimed to evaluate the protective effects of a novel anti-hyperglycemic “Mcy protein” isolated from the fruits of Momordica cymbalaria in streptozotocin induced- diabetes rat model. Materials and Methods: Wild type and Streptozotocin induced diabetic male wistar albino rats were either treated with single intraperitoneal injection of 2.5 mg Mcy protein/kg body weight or acetate buffer daily for 30 days. Fasting blood glucose and, serum and tissue lipid levels were measured along with biochemical analysis for hepatic and renal function tests. Results: Mcy protein significantly reduced the fasting blood glucose and, serum as well as tissue lipid levels (p<0.05), besides normalizing the levels of liver and kidney function markers in the treated diabetic rats when compared to the diabetic controls. Our studies also showed the pancreatic islet regeneration in Mcy treated rats. Conclusion: Mcy protein can alleviate hyperlipidemia and help manage diabetes by stimulating insulin secretion without evident toxic effects on liver and kidney.

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Ameliorative effect of Albizzia procera leaves extract against experimentally induced gastric ulcer models in Wistar albino rats

Journal of Applied and Natural Science ◽

10.31018/jans.v13i1.2513 ◽

2021 ◽

Vol 13 (1) ◽

pp. 308-315

Author(s):

Vivek Srivastava ◽

Santosh Kumar Verma ◽

Neha Mathur ◽

Kapil Kumar Goel

Keyword(s):

Gastric Ulcer ◽

Ethanolic Extract ◽

Gastric Fluid ◽

Gastric Ulcers ◽

Albino Rats ◽

Traditional Medicines ◽

Pylorus Ligation ◽

Ameliorative Effect ◽

Wistar Albino Rats ◽

Experimentally Induced

Albizzia procera belongs to family Fabaceae and has several phytoconstituents like flavonoids, terpenes, alkaloids, saponins etc. The plant is commonly used in traditional medicines. The objective of the present study was to assess the ameliorative effect of A. procera leaves extract against an experimentally induced gastric ulcer in albino rats. The antiulcer, antisecretory and cytoprotective properties of an ethanolic extract prepared from the leaves was evaluated. When given in a 200 mg/kg oral (per. os) dose, the extract produced 74% and 85% protection index in ethanol-induced ulcer model and pylorus ligation model, respectively. Additionally, the extract also prohibited the formation of haemorrhage and edema, significantly lessened catalase activity (p<0.0001) and the lipid peroxidation level (p<0.0001) in the glandular tissue of Wistar albino rats. Furthermore, the extract also significantly diminished the total acidity(p<0.0001) of the gastric fluid by increasing its pH (p<0.0001). The occupancy of different phytoconstituents in the extract, such as flavonoids and tannins were identified that may be responsible for its gastro-protective activity. All these results provide a basis explaining the antiulcer ability of A. procera being useful in the management of gastric ulcers.

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