Utilization of induction furnace steel slag based iron oxide nanocomposites for antibacterial studies

AbstractMetals and metal oxide-based nanocomposites play a significant role over the control of microbes. In this study, antibacterial activity of iron oxide (Fe2O3) nanocomposites based on induction furnace (IF) steel slag has been carried out. IF steel slag is an industrial by-product generated from secondary steel manufacturing process and has various metal oxides which includes Al2O3 (7.89%), MnO (5.06), CaO (1.49%) and specifically Fe2O3 (14.30%) in higher content along with metalloid SiO2 (66.42). Antibacterial activity of iron oxide nanocomposites has been revealed on bacterial species such as Micrococcus luteus, Bacillus subtilis and Staphylococcus aureus. Micrococcus luteus has undergone maximum zone of inhibition (ZOI) of 12 mm for 10 mg/mL concentration of steel slag iron oxide nanocomposite. Growth inhibitory kinetics of bacterial species has been studied using ELISA microplate reader at 660 nm by varying the concentration of steel slag iron oxide nanocomposites. The results illustrate that IF steel slag is a potential material and can be utilized in building materials to increase the resistance against biodeterioration. Graphic abstract

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COMPARATIVE ANALYSIS OF METAL NANOPARTICLES SYNTHESIZED FROM HIBISCUS ROSA SINESIS AND THEIR ANTIBACTERIAL ACTIVITY ESTIMATION AGAINST NINE PATHOGENIC BACTERIA

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2017.v10i5.17458 ◽

2017 ◽

Vol 10 (5) ◽

pp. 323

Author(s):

Shruti Tyagi

Keyword(s):

Antibacterial Activity ◽

Pathogenic Bacteria ◽

Au Nanoparticles ◽

Size Range ◽

Micrococcus Luteus ◽

Bacterial Species ◽

Cost Effective ◽

Antibacterial Activities ◽

Agar Disc ◽

Vis Spectroscopy

Objective: This study demonstates a simple, cost effective protocol for biosynthesis of stable silver (Ag) and gold (Au) nanoparticles from Hibiscus Rosa sinesis and their comparison by applying antibacterial activities against nine pathogenic bacterial species.Methods: Silver (Ag) and gold (Au) nanoparticles were biosynthesized from Hibiscus Rosa sinesis were characterized by UV–VIS spectroscopy, FTIR and TEM. The antibacterial activities of AgNPs and AuNPs were evaluated against 9 pathogenic bacterial species Pseudomonas aeroginosa, Bacillus subtilis Micrococcus luteus, Staphylococcus aureus, Staphylococcus epidermidis, Enterobacter aerogens, Escherichia coli, Streptococcus pneumoniae, Aeromonas hydrophila by the agar disc diffusion method.Results: Synthesized AgNPs were obtained in 13.01 to 28.14 nm size range, while AuNPs were in 6.32 to 18.19 nm size range. The results of Fourier transform infrared spectroscopy (FTIR) spectra indicates that the AuNPs are bound to amine groups and the AgNPs to carboxylate ion groups. The antibacterial activities of AgNPs, the zone of inhibition significantly increased with the increases of concentrations of AgNPs in all pathogenic bacterial species except in the case of S. epidermidis at 50%, S. aerogenes and A. hydrophila at 70%, while in case of AuNPs antibacterial activity was displayed only against B. subtilis at 20% and 100% concentration.Conclusion: This study suggests that AgNPs exhibits outstanding antibacterial activity against pathogenic bacteria as compared to AuNPs synthesized from Hibiscus Rosa sinensis leaf extract and insights to their potential applicability as an alternative antibacterial agent in microbial and human health system to reduce the resistance ability of pathogenic bacteria. Keywords: silver nanoparticles; gold nanoparticles; UV–VIS spectroscopy; FTIR; TEM; antibacterial activities.

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Antibacterial Activity of Copper and Cobalt Amino Acids Complexes

Notulae Botanicae Horti Agrobotanici Cluj-Napoca ◽

10.15835/nbha3926847 ◽

2011 ◽

Vol 39 (2) ◽

pp. 124 ◽

Cited By ~ 22

Author(s):

ANDREEA STĂNILĂ ◽

Cornelia BRAICU ◽

Sorin STĂNILĂ ◽

Raluca M. POP

Keyword(s):

Escherichia Coli ◽

Amino Acids ◽

Antibacterial Activity ◽

Bacillus Cereus ◽

Copper Complexes ◽

Micrococcus Luteus ◽

Bacterial Species ◽

Free Ligand ◽

Antibacterial Properties ◽

Moderate Activity

The antibacterial properties of differently copper and cobalt amino acids complexes on agar plates was investigated in the present study. The antibacterial activity of amino acid complexes was evaluated against on three bacteria strains (Escherichia coli, Bacillus cereus, Micrococcus luteus). Generally, the amino acids complexes were mainly active against gram-positive organisms, species like Micrococcus luteus being the most susceptible strain tested. It was registered a moderate antibacterial activity against Bacillus cereus. The microorganisms Escherichia coli, which are already known to be multi-resistant to drugs, were also resistant to the amino acids complexes but also to the free salts tested. Escherichia coli were susceptible only to the CoCl2 and copper complex with phenylalanine. The complexes with leucine and histidine seem to be more active than the parent free ligand against one or more bacterial species. Moderate activity was registered in the case of complexes with methionine and phenylalanine. From the complexes tested less efficient antibacterial activity was noted in the case of complexes with lysine and valine. These results show that cobalt and copper complexes have an antibacterial activity and suggest their potential application as antibacterial agents.

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Chemical Constituents and Antibacterial Activity of Essential Oils of Needles of Pinus Sylvestris (Scots Pine) from South West Nigeria

10.21203/rs.3.rs-635195/v1 ◽

2021 ◽

Author(s):

Kehinde A. Oyewole ◽

Omotayo O. Oyedara ◽

Shola H. Awojide ◽

Mary O. Olawade ◽

Charles O. Adetunji

Keyword(s):

Antibacterial Activity ◽

Fatty Acid ◽

Pinus Sylvestris ◽

Scots Pine ◽

Micrococcus Luteus ◽

Chemical Constituents ◽

Bacterial Species ◽

Antibacterial Properties ◽

Biological Properties ◽

Klebsiella Pneumonia

Abstract Pinus sylvestris (Scots pine) is a coniferous plant that possesses different biological properties such as antiseptic, antioxidant, antifungal, and anti-inflammatory. This study investigated the chemical constituents and in vitro antibacterial activities of essential oil (EO) of Pinus sylvestris (Scots pine) against thirteen bacterial species using Gas chromatography/mass spectrophotometry (GC/MS) and standard agar well diffusion assay. The EO exhibited excellent antibacterial activity inhibiting all the bacterial isolates tested with the diameter zone of inhibitions (Zi) ranging from 8–24 mm. The minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) ranged between 0.025mg/ml and 25.000mg/ml. The highest antibacterial activity was recorded against Micrococcus luteus NCIB 196 and Klebsiella pneumonia NCIB 418 (Zi = 24 mm), while the lowest activity was recorded against Proteus vulgaris (Zi = 8 mm). The GC/MS analysis revealed the presence of 30 chemical compounds, of which seven possess antibacterial properties. These include three oxygenated monoterpenes (α-terpineol, borneol, and fenchol), two sesquiterpenes (caryophyllene and δ-cadinene), one saturated fatty acid (palmitic acid) and monounsaturated fatty acid (oleic acids). Therefore, the results suggest the potential of P. sylvestris as an antimicrobial agent for incorporation in the treatment of pathogens.

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Acalypha Indica mediated MgO NPs: A novel approach in greener route with its antibacterial activity against pathogens

Nanoscale Reports ◽

10.26524/nr.3.12 ◽

2020 ◽

Vol 3 (2) ◽

Author(s):

Kavipriya K C ◽

Sudha A P ◽

Sujatha K ◽

Sowmya Lakshmi K

Keyword(s):

Antibacterial Activity ◽

Metal Oxides ◽

Bacterial Species ◽

Magnesium Nitrate ◽

Precipitation Method ◽

Average Crystalline Size ◽

Face Centered Cubic ◽

X Ray ◽

Novel Approach ◽

Acalypha Indica

The interest in miniaturization of particles revealed the hidden applications of metal oxides. The potential applications of the particles may vary when the size of the particle is reduced. One of the alternative routes to the conventional approach is the use of plant extract for the synthesis of metal oxides NPs. In the framework of this study, the ecofriendly MgO nanoparticles were synthesized using Acalypha Indica leaf extract,functioning as reducing and capping agent by co-precipitation method. The predecessor taken here was Magnesium Nitrate. The biologically synthesized MgO NPs were characterized by various techniques like X ray diffraction(XRD), Fourier Transform infrared spectroscopy(FTIR), Scanning electron microscope (SEM) with Energy Dispersive X-ray spectroscopy(EDX) profile and its antibacterial activity is evaluated against causative organisms. XRD studies confirmed the face centered cubic crystalline structure of MgO NPs and the average crystalline size of MgO NPs calculated using Scherer’s formula was found to be 13 nm. FTIR spectrum shows a significant Mg-O vibrational band. Purity, surface morphology and chemical composition of elements were confirmed by SEM with EDX. The SEM result shows the fine spherical morphology with the grain size range between 43nm to 62nm. Antimicrobial assay of MgO NPs was examined against gram positive and negative bacteria. Appreciated activity was observed on the Staphylococcus aureus bacterial species. In general, the renewed attempt of this facile approach gave the optimum results of multifunctional MgO NPs.

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Investigation on the antibacterial activity of electronic cigarette liquids (ECLs): a proof of concept study

Current Pharmaceutical Biotechnology ◽

10.2174/1389201021666200903121624 ◽

2020 ◽

Vol 21 ◽

Cited By ~ 1

Author(s):

Virginia Fuochi ◽

Massimo Caruso ◽

Rosalia Emma ◽

Aldo Stivala ◽

Riccardo Polosa ◽

...

Keyword(s):

Antibacterial Activity ◽

Bacterial Species ◽

A549 Cells ◽

Bactericidal Effect ◽

Minimal Bactericidal Concentration ◽

Viability Assay ◽

Dependent Inhibition ◽

Dose Dependent Inhibition ◽

Dose Dependent

Background: The key ingredients of e-cigarettes liquid are commonly propane-1,2-diol (also called propylene glycol) and propane-1,2,3-triol (vegetal glycerol) and their antimicrobial effects are already established. The nicotine and flavors which are often present in e-liquids can interfere with the growth of some microorganisms. Objective: The effect of the combining these elements in e-liquids is unknown. The aim of the study was to investigate the possible effects of these liquids on bacterial growth in the presence or absence of nicotine and flavors. Methods: Susceptibilities of pathogenic strains (Klebsiella pneumoniae, Staphylococcus aureus, Pseudomonas aeruginosa, Acinetobacter baumannii, Escherichia coli, Enterococcus faecalis and Sarcina lutea) were studied by means of a multidisciplinary approach. Cell viability and antioxidant assays were also evaluated. Results: All e-liquids investigated showed antibacterial activity against at least one pathogenic strain. A higher activity was correlated to the presence of flavors and nicotine. Discussion: In most cases the value of minimal bactericidal concentration is equal to the value of minimal inhibitory concentration showing that these substances have a bactericidal effect. This effect was observed in concentrations up to 6.25% v/v. Antioxidant activity was also correlated to presence of flavors. Over time, the viability assay in human epithelial lung A549 cells showed a dose-dependent inhibition of cell growth. Conclusion: Our results have shown that flavors considerably enhance the antibacterial activity of propane-1,2-diol and propane-1,2,3-triol. This study provides important evidence that should be taken into consideration in further investigative approaches, to clarify the different sensitivity of the various bacterial species to e-liquids, including the respiratory microbiota, to highlight the possible role of flavors and nicotine.

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Iso-octenidine: Promising Octenidine Analogue with Improved Solubility

Current Organic Synthesis ◽

10.2174/1570179417666201231104453 ◽

2020 ◽

Vol 17 ◽

Author(s):

Igor K. Yakuschenko ◽

Nataliya N. Pozdeeva ◽

Viktoriya A. Mumyatova ◽

Alexey A. Terentiev ◽

Svyatoslav Ya. Gadomsky

Keyword(s):

Escherichia Coli ◽

Antibacterial Activity ◽

Micrococcus Luteus ◽

Octenidine Dihydrochloride ◽

First Time

: Iso-octenidine, an isomer of octenidine dihydrochloride, was synthesized and studied for the first time. Isooctenidine was demonstrated to be 3-fold more soluble in water in comparison to original octenidine, and both substances had remarkably similar antibacterial activity (tested on Escherichia Coli and Micrococcus luteus).

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Synthesis of iron oxide nanoparticles by mixing chilli with rust iron extract to examine antibacterial activity

Materials Technology ◽

10.1080/10667857.2021.1959189 ◽

2021 ◽

pp. 1-10

Author(s):

Muslim A. Abid ◽

Duha A. Kadhim

Keyword(s):

Antibacterial Activity ◽

Iron Oxide ◽

Iron Oxide Nanoparticles ◽

Oxide Nanoparticles

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Evaluation of Commercial Disinfectants against Staphylococcus lentus and Micrococcus spp. of Poultry Origin

Veterinary Medicine International ◽

10.1155/2020/8811540 ◽

2020 ◽

Vol 2020 ◽

pp. 1-10

Author(s):

Otun Saha ◽

Nadira Naznin Rakhi ◽

Arif Istiaq ◽

Israt Islam ◽

Munawar Sultana ◽

...

Keyword(s):

Micrococcus Luteus ◽

Bacterial Species ◽

16S Rrna Gene Sequencing ◽

Average Diameter ◽

Diffusion Method ◽

Dilution Method ◽

Rrna Gene ◽

Poultry Farms ◽

Rrna Gene Sequencing ◽

Selection Of

Introduction. Effective sanitation strategies for poultry farms require an appropriate selection of the disinfectant based on the contaminants present and their sensitivity to the disinfectants. Aim. The current study investigated the prevalence of streptococci/micrococci in poultry farms of Bangladesh and the efficacy of commercial disinfectants (Savlon, Lysol, Quatovet, Virkon S, and Virocid) along with alcohol against these pathogens to adopt appropriate strategies. Materials and Methods. Conventional approaches and the 16S rRNA gene sequencing were performed to confirm the isolates at the species level along with microtiter biofilm assay to determine their biofilm-forming ability. Efficacy of the disinfectants was tested against those isolates using agar well diffusion and minimum inhibitory concentration (MIC) test by broth dilution method using different dilutions of the disinfectants. Results. Staphylococcus lentus (n = 32), Micrococcus luteus (n = 7), and Micrococcus aloeverae (n = 4) were confirmed among 102 presumptively screened streptococci/micrococci isolates from 43 samples. No single disinfectant showed equally high efficacy against all three bacterial species in agar well diffusion test, although Virocid showed the lowest MIC against all three of them. Lysol was least effective among the commercial disinfectants by both MIC and diffusion method, although each commercial disinfectant was more effective than alcohol. Considering both the average diameter of the inhibition zones and the MIC values, efficacy can be interpreted as Virocid > Quatovet > Savlon > Virkon S > Lysol. Although the efficacy decreased with decreasing concentration, the disinfectants retained a satisfactory level of efficacy at 50% concentration. Among test pathogens, M. aloeverae was the most sensitive to the disinfectants and the weakest biofilm producers, whereas 4/14 S. lentus and 1/5 M. luteus were strong biofilm producers, which may cause more reduction in the efficacy in environmental conditions. Conclusion. As no ideal disinfectant was found in the study, the efficacy of the disinfectants should be routinely evaluated and validated to ensure the sanitation standards in the poultry sector.

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Development of a Cavity Disinfectant Containing Antibacterial Monomer MDPB

Journal of Dental Research ◽

10.1177/0022034516663465 ◽

2016 ◽

Vol 95 (13) ◽

pp. 1487-1493 ◽

Cited By ~ 14

Author(s):

N. Hirose ◽

R. Kitagawa ◽

H. Kitagawa ◽

H. Maezono ◽

A. Mine ◽

...

Keyword(s):

Antibacterial Activity ◽

Bond Strength ◽

Bacterial Species ◽

Oral Bacteria ◽

Resin Cement ◽

Resin Cements ◽

Adhesive Resin ◽

Significant Difference ◽

Adhesive Resin Cement

An experimental cavity disinfectant (ACC) that is intended to be used for various direct and indirect restorations was prepared by adding an antibacterial monomer 12-methacryloyloxydodecylpyridinum bromide (MDPB) at 5% into 80% ethanol. The antibacterial effectiveness of ACC and its influences on the bonding abilities of resin cements were investigated. To examine the antibacterial activity of unpolymerized MDPB, the minimum inhibitory and bactericidal concentrations (MIC and MBC) were determined for Streptococcus mutans, Lactobacillus casei, Actinomyces naeslundii, Parvimonas micra, Enterococcus faecalis, Fusobacterium nucleatum, and Porphyromonas gingivalis. Antibacterial activities of ACC and the commercial cavity disinfectant containing 2% chlorhexidine and ethanol (CPS) were evaluated by agar disk diffusion tests through 7 bacterial species and by MIC and MBC measurement for S. mutans. The effects of ACC and CPS to kill bacteria in dentinal tubules were compared with an S. mutans–infected dentin model. Shear bond strength tests were used to examine the influences of ACC on the dentin-bonding abilities of a self-adhesive resin cement and a dual-cure resin cement used with a primer. Unpolymerized MDPB showed strong antibacterial activity against 7 oral bacteria. ACC produced inhibition zones against all bacterial species similar to CPS. For ACC and CPS, the MIC value for S. mutans was identical, and the MBC was similar with only a 1-step dilution difference (1:2). Treatment of infected dentin with ACC resulted in significantly greater bactericidal effects than CPS ( P < 0.05, analysis of variance and Tukey’s honest significant difference test). ACC showed no negative influences on the bonding abilities to dentin for both resin cements, while CPS reduced the bond strength of the self-adhesive resin cement ( P < 0.05). This study clarified that the experimental cavity disinfectant containing 5% MDPB is more effective in vitro than the commercially available chlorhexidine solution to eradicate bacteria in dentin, without causing any adverse influences on the bonding abilities of resinous luting cements.

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