scholarly journals Chemical Constituents and Antibacterial Activity of Essential Oils of Needles of Pinus Sylvestris (Scots Pine) from South West Nigeria

2021 ◽  
Author(s):  
Kehinde A. Oyewole ◽  
Omotayo O. Oyedara ◽  
Shola H. Awojide ◽  
Mary O. Olawade ◽  
Charles O. Adetunji
Keyword(s):  
Antibacterial Activity ◽  
Fatty Acid ◽  
Pinus Sylvestris ◽  
Scots Pine ◽  
Micrococcus Luteus ◽  
Chemical Constituents ◽  
Bacterial Species ◽  
Antibacterial Properties ◽  
Biological Properties ◽  
Klebsiella Pneumonia

Abstract Pinus sylvestris (Scots pine) is a coniferous plant that possesses different biological properties such as antiseptic, antioxidant, antifungal, and anti-inflammatory. This study investigated the chemical constituents and in vitro antibacterial activities of essential oil (EO) of Pinus sylvestris (Scots pine) against thirteen bacterial species using Gas chromatography/mass spectrophotometry (GC/MS) and standard agar well diffusion assay. The EO exhibited excellent antibacterial activity inhibiting all the bacterial isolates tested with the diameter zone of inhibitions (Zi) ranging from 8–24 mm. The minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) ranged between 0.025mg/ml and 25.000mg/ml. The highest antibacterial activity was recorded against Micrococcus luteus NCIB 196 and Klebsiella pneumonia NCIB 418 (Zi = 24 mm), while the lowest activity was recorded against Proteus vulgaris (Zi = 8 mm). The GC/MS analysis revealed the presence of 30 chemical compounds, of which seven possess antibacterial properties. These include three oxygenated monoterpenes (α-terpineol, borneol, and fenchol), two sesquiterpenes (caryophyllene and δ-cadinene), one saturated fatty acid (palmitic acid) and monounsaturated fatty acid (oleic acids). Therefore, the results suggest the potential of P. sylvestris as an antimicrobial agent for incorporation in the treatment of pathogens.

scholarly journals Antibacterial Activity of Copper and Cobalt Amino Acids Complexes

2011 ◽  
Vol 39 (2) ◽  
pp. 124 ◽  
Author(s):  
ANDREEA STĂNILĂ ◽  
Cornelia BRAICU ◽  
Sorin STĂNILĂ ◽  
Raluca M. POP
Keyword(s):  
Escherichia Coli ◽  
Amino Acids ◽  
Antibacterial Activity ◽  
Bacillus Cereus ◽  
Copper Complexes ◽  
Micrococcus Luteus ◽  
Bacterial Species ◽  
Free Ligand ◽  
Antibacterial Properties ◽  
Moderate Activity

The antibacterial properties of differently copper and cobalt amino acids complexes on agar plates was investigated in the present study. The antibacterial activity of amino acid complexes was evaluated against on three bacteria strains (Escherichia coli, Bacillus cereus, Micrococcus luteus). Generally, the amino acids complexes were mainly active against gram-positive organisms, species like Micrococcus luteus being the most susceptible strain tested. It was registered a moderate antibacterial activity against Bacillus cereus. The microorganisms Escherichia coli, which are already known to be multi-resistant to drugs, were also resistant to the amino acids complexes but also to the free salts tested. Escherichia coli were susceptible only to the CoCl2 and copper complex with phenylalanine. The complexes with leucine and histidine seem to be more active than the parent free ligand against one or more bacterial species. Moderate activity was registered in the case of complexes with methionine and phenylalanine. From the complexes tested less efficient antibacterial activity was noted in the case of complexes with lysine and valine. These results show that cobalt and copper complexes have an antibacterial activity and suggest their potential application as antibacterial agents.

scholarly journals Utilization of induction furnace steel slag based iron oxide nanocomposites for antibacterial studies

2021 ◽  
Vol 3 (3) ◽  
Author(s):  
J. Baalamurugan ◽  
V. Ganesh Kumar ◽  
T. Stalin Dhas ◽  
S. Taran ◽  
S. Nalini ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Iron Oxide ◽  
Building Materials ◽  
Induction Furnace ◽  
Micrococcus Luteus ◽  
Bacterial Species ◽  
Steel Slag ◽  
If Steel ◽  
Growth Inhibitory ◽  
Microplate Reader

AbstractMetals and metal oxide-based nanocomposites play a significant role over the control of microbes. In this study, antibacterial activity of iron oxide (Fe2O3) nanocomposites based on induction furnace (IF) steel slag has been carried out. IF steel slag is an industrial by-product generated from secondary steel manufacturing process and has various metal oxides which includes Al2O3 (7.89%), MnO (5.06), CaO (1.49%) and specifically Fe2O3 (14.30%) in higher content along with metalloid SiO2 (66.42). Antibacterial activity of iron oxide nanocomposites has been revealed on bacterial species such as Micrococcus luteus, Bacillus subtilis and Staphylococcus aureus. Micrococcus luteus has undergone maximum zone of inhibition (ZOI) of 12 mm for 10 mg/mL concentration of steel slag iron oxide nanocomposite. Growth inhibitory kinetics of bacterial species has been studied using ELISA microplate reader at 660 nm by varying the concentration of steel slag iron oxide nanocomposites. The results illustrate that IF steel slag is a potential material and can be utilized in building materials to increase the resistance against biodeterioration. Graphic abstract

scholarly journals COMPARATIVE ANALYSIS OF METAL NANOPARTICLES SYNTHESIZED FROM HIBISCUS ROSA SINESIS AND THEIR ANTIBACTERIAL ACTIVITY ESTIMATION AGAINST NINE PATHOGENIC BACTERIA

2017 ◽  
Vol 10 (5) ◽  
pp. 323
Author(s):  
Shruti Tyagi
Keyword(s):  
Antibacterial Activity ◽  
Pathogenic Bacteria ◽  
Au Nanoparticles ◽  
Size Range ◽  
Micrococcus Luteus ◽  
Bacterial Species ◽  
Cost Effective ◽  
Antibacterial Activities ◽  
Agar Disc ◽  
Vis Spectroscopy

Objective: This study demonstates  a simple, cost effective protocol  for biosynthesis of stable silver (Ag) and gold (Au) nanoparticles from Hibiscus Rosa sinesis and their comparison by applying antibacterial activities against nine pathogenic bacterial species.Methods: Silver (Ag) and gold (Au) nanoparticles were biosynthesized from Hibiscus Rosa sinesis were characterized by UV–VIS spectroscopy, FTIR and TEM. The antibacterial activities  of AgNPs  and AuNPs were evaluated against  9 pathogenic bacterial species  Pseudomonas aeroginosa, Bacillus subtilis Micrococcus luteus, Staphylococcus aureus, Staphylococcus epidermidis, Enterobacter aerogens, Escherichia coli, Streptococcus pneumoniae, Aeromonas hydrophila by the agar disc diffusion method.Results: Synthesized AgNPs  were obtained in 13.01 to 28.14 nm size range, while AuNPs were in  6.32 to 18.19 nm size range. The results of Fourier transform infrared spectroscopy (FTIR) spectra indicates  that the AuNPs are bound to amine groups and the AgNPs to carboxylate ion groups. The antibacterial activities  of AgNPs,  the zone of inhibition significantly increased with the  increases of concentrations of AgNPs in all pathogenic bacterial species  except  in the case of S. epidermidis at 50%, S. aerogenes and A. hydrophila at 70%, while in case of AuNPs antibacterial activity  was displayed  only against B. subtilis at 20% and 100% concentration.Conclusion: This study suggests that AgNPs exhibits outstanding antibacterial activity against pathogenic bacteria as compared to AuNPs synthesized from Hibiscus Rosa sinensis leaf extract and insights to their potential applicability as an alternative antibacterial  agent in microbial and human health system to reduce the resistance ability of pathogenic bacteria. Keywords: silver nanoparticles; gold  nanoparticles; UV–VIS spectroscopy; FTIR; TEM; antibacterial activities.  

scholarly journals Prevalence of Bacterial Vaginosis among Married Women in Kalar District, Iraqi Kurdistan Region

passer ◽  
2019 ◽  
Vol 3 (2) ◽  
pp. 194-199
Author(s):  
Saman Mohammed Mohammed- Amin
Keyword(s):  
Bacterial Vaginosis ◽  
Pathogenic Bacteria ◽  
Clinical Symptoms ◽  
Micrococcus Luteus ◽  
Bacterial Species ◽  
Married Women ◽  
P Value ◽  
Klebsiella Pneumonia ◽  
Microbiological Analysis ◽  
Isolation And Identification

Abstract Bacterial vaginosis (BV) is an inflammatory disease, caused by polymicrobial infection, including pathogenic bacteria which replace the vaginal normal flora and finally this replacement causes manifestations of several physiological and clinical symptoms among women within different ages. BV has become one of the main problems that make woman patients visit gynecological and obstetric consultant hospitals in most country. The present study is designed to determine the causative pathogen and the prevalence of bacterial vaginosis among married women patients in Kalar district. This cross-sectional study was performed from the beginning of March to the mid April-2021 among women who attended Obstetrics and Gynecological governmental hospital and out-patient clinics in Kalar City. Intra vaginal swabs have been collected in sterile Amies transport medium sticks and processed for isolation and identification of bacterial species depending on colony morphology, Gram’s stain and microbiological analysis protocols. Then socio-demographic and gynaecologic data were collected by questionnaire. Out of the 108 participant women who suffered from Gynecological diseases, 67(62.03%) of them exhibited bacterial vaginosis. From the 73 different isolated colonies, 18 bacterial species were identified; coagulase-negative staphylococci (CoNS) were the predominant cause of BV (32.84%), followed by E. coli (14.93%), Staphylococcus aureus (13.43%), Klebsiella pneumonia (8.96) and Micrococcus luteus (7.46%), while Proteus spp. and some uncommon bacteria display (1.49%) for each of them. The socio-demographic analysis between positive and negative woman patients revealed that the association between all studied risk factors and BV were statistically significant (P value < 0.05) except the age factor which was statistically non-significant meaning that the age was not associated with BV. In addition, the clinical symptom analysis showed that abnormal vaginal discharge, lower back pain, dysmenorrhea and strawberry were significantly associated with BV (P value < 0.05), while the rest of other factor did not exhibit statistically significant association.

scholarly journals Bioactive Properties of the Aqueous Extracts of Endophytic Fungi Associated with Scots Pine (Pinus sylvestris) Roots

Planta Medica ◽  
2020 ◽  
Vol 86 (13/14) ◽  
pp. 1009-1024
Author(s):  
Jenni Tienaho ◽  
Maarit Karonen ◽  
Riina Muilu-Mäkelä ◽  
Janne Kaseva ◽  
Nuria de Pedro ◽  
...  
Keyword(s):  
Pinus Sylvestris ◽  
Macular Degeneration ◽  
Scots Pine ◽  
Endophytic Fungi ◽  
Antibacterial Properties ◽  
Biologically Active ◽  
Age Related Macular Degeneration ◽  
Aqueous Extracts ◽  
Phialocephala Fortinii ◽  
Age Related

AbstractDespite the continuing interest in various plant and natural products, only a small portion of the biologically active compounds from nature has been discovered and exploited. In this study, antioxidant and antibacterial properties of aqueous fractions of three endophytic fungi isolated from the roots of 8-year-old Scots pines (Pinus sylvestris) growing on a drained peatland were investigated. The endophytic fungi species were Acephala applanata, Phialocephala fortinii, and Humicolopsis cephalosporioides/Coniochaeta mutabilis. The bioactivities were examined using hydrogen peroxide scavenging and oxygen radical absorbance capacity tests as well as sensitive Escherichia coli-based biosensors, which produce a luminescent signal in the presence of substances with oxidative or genotoxic properties. In addition, cell models for Parkinsonʼs disease, age-related macular degeneration, and osteoarthritis were used to evaluate the potential for pharmaceutical applications. The aqueous extracts of fungi and 19 out of 42 fractions were found to be active in one or more of the tests used. However, no activity was found in the age-related macular degeneration and osteoarthritis cell model tests. Additionally, bioactivity data was connected with metabolites putatively annotated, and out of 330 metabolites, 177 were interesting in view of the bioactivities investigated. A majority of these were peptides and all three fungal species shared a highly similar metabolome. We propose that Scots pine endophytic fungi are a rich source of interesting metabolites, and synergistic effects may cause the bioactivities, as they were found to vary after the fractionation process.

scholarly journals Antibacterial Properties of Polysulfone Membranes Blended with Arabic Gum

Membranes ◽  
2019 ◽  
Vol 9 (2) ◽  
pp. 29 ◽  
Author(s):  
Souhir Sabri ◽  
Ahmad Najjar ◽  
Yehia Manawi ◽  
Nahla Eltai ◽  
Asma Al-Thani ◽  
...  
Keyword(s):  
Bacterial Colonization ◽  
Membrane Surface ◽  
Bacterial Species ◽  
Antibacterial Properties ◽  
Bacterial Attachment ◽  
Inversion Method ◽  
Klebsiella Pneumonia ◽  
Gram Positive ◽  
Arabic Gum ◽  
Gram Negative

Polysulfone (PS) membranes blended with different loadings of arabic gum (AG) were synthesized using phase inversion method and the antibacterial properties of the synthesized membranes were tested using a number Gram-negative (Escherichia coli, Klebsiella pneumonia and Pseudomonas aeruginosa) and Gram-positive (Staphylococcus aureus) bacterial species. It was shown that AG addition to the dope polymer solutions essentially changed porous structure, hydrophilicity and zeta potential of the cast PS/AG membranes. These changes were due to the amphiphilic properties of AG macromolecules that contained negatively charged hydrophilic residues. A pronounced decrease in bacterial attachment was seen in the field emission scanning electron microscopy (FESEM) images for PS/AG membrane samples compared to both commercial (Microdyn-Nadir) and bare PS (without AG) membranes. AG loading dependent trend was observed where the prevention of bacterial colonization on the membrane surface was strongest at the highest (7 wt. %) AG loading in the casting solution. Possible mechanisms for the prevention of bacterial colonization were discussed. Significantly, the inhibition of bacterial attachment and growth on PS/AG membranes was observed for both Gram-positive and Gram-negative bacterial models, rendering these novel membranes with strong biofouling resistance attractive for water treatment applications.

(−)-Brunneusine, a new phenolic compound with antibacterial properties in aqueous medium from the leaves of Agelanthus brunneus (Engl.) Tiegh (LORANTHACEAE)

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Moifo Kuete Thomas Wieland ◽  
Ambassa Pantaleon ◽  
Moungang Luciane Marlyse ◽  
Ngameni Bathelemy ◽  
Storr E. Thomas ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Aqueous Medium ◽  
Phenolic Compound ◽  
Aquatic Environment ◽  
Bacterial Species ◽  
Antibacterial Properties ◽  
Chemical Investigation ◽  
Senna Siamea ◽  
Bactericidal Effects

Abstract Agelanthus brunneus (Loranthaceae) is a hemiparasitic plant growing on Senna siamea (Fabaceae). The chemical investigation of its leaves and flowers led to the isolation of one new phenolic compound namely (−)-brunneusine (1), together with 13 known compounds. The crude leaves and flowers extracts (CLE and CFLE) with their ethyl acetate fractions (EAFL and EAFFL) and some isolated compounds (1–3; 8–9 and 11–14) have been tested on four bacterial species of sanitary importance isolated in an aquatic environment. All the samples except compound 3 showed antibacterial activity with MICs ranging from 0.43 to 8.88.103 µg/mL and MBCs from 0.43 to 3.55.103 µg/mL. Compounds 9 and 14 showed better activity on all bacterial species tested with MICs ranging from 0.43 to 27.77 µg/mL. Only CLE, EAFL and compounds 14, 2, 8 and 9 showed bactericidal effects on all bacterial species tested.

Lipids and their seasonal variation in the nucellar layers of Scots pine (Pinus sylvestris) seeds

1996 ◽  
Vol 74 (9) ◽  
pp. 1416-1424 ◽  
Author(s):  
Eila Tillman-Sutela ◽  
Anu Johansson ◽  
Pälvi Laakso ◽  
Heikki Kallio ◽  
Anneli Kauppi
Keyword(s):  
Fatty Acids ◽  
Seasonal Variation ◽  
Fatty Acid ◽  
Pinus Sylvestris ◽  
Scots Pine ◽  
Unsaturated Fatty Acids ◽  
Saturated Fatty Acids ◽  
Lipid Fraction ◽  
Single Fatty Acid

The content, composition, and seasonal variation of lipids in the nucellar layers of two mature Scots pine seed lots from northern and central Finland were studied. These lipophilic layers, which are located inside the seed coat, are composed of collapsed cells and restrict the imbibition of seeds. In this study, epicuticular wax was found on their surface; in particular, the surface of the nucellar cap was composed of intermeshing wax rods. The content of total lipids of the nucellar layers varied seasonally from 10 to 24%, being highest in April. In addition to simple lipids, which formed the most abundant lipid fraction in nucellar layers, small amounts of phospho- and glyco-lipids were also present. The proportion of simple lipids and their fatty acid composition in the seeds of both provenances remained constant throughout the year, whereas seasonal variation was found in the proportions of phospho- and giyco-lipids and in their fatty acids. In general, the variation was more marked in the northern seed lot. The unsaturated fatty acids dominated in the fractions of simple lipids and phospholipids, with linoleic acid being the most abundant single fatty acid. The saturated fatty acids with more than 22 carbon atoms were most abundant in the glycolipid fractions. The role of lipids for the function of the collapsed nucellar layers in the regulation of imbibition and germination of pine seeds is discussed. Keywords: Pinus sylvestris L., northern seeds, fatty acids, imbibition, germination.

scholarly journals Effect of Silver Nanoparticles on Physicochemical and Antibacterial Properties of Calcium Silicate Cements

2016 ◽  
Vol 27 (5) ◽  
pp. 508-514 ◽  
Author(s):  
Fernando Vazquez-Garcia ◽  
◽  
Mário Tanomaru-Filho ◽  
Gisselle Moraima Chávez-Andrade ◽  
Roberta Bosso-Martelo ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Silver Nanoparticles ◽  
Compressive Strength ◽  
Antibacterial Activity ◽  
Calcium Silicate ◽  
Setting Time ◽  
Antibacterial Properties ◽  
Mineral Trioxide Aggregate ◽  
Biological Properties ◽  
Calcium Silicate Cements

Abstract Mineral trioxide aggregate (MTA) and Portland cement (PC) are calcium silicate cements. They have similar physicochemical, mechanical and biological properties. The addition of zirconium oxide (ZrO2) to PC provides radiopacity. Silver nanoparticles (AgNPs) may improve some properties of cements. The aim of this study was to evaluate the effect of AgNPs on physicochemical/mechanical properties and antibacterial activity of white MTA (WMTA) and PC associated with ZrO2. The following materials were evaluated: WMTA; PC 70% + ZrO2 30%; WMTA+ AgNPs; and PC 70% + ZrO2 30% + AgNPs. The study evaluated radiopacity, setting time, pH, compressive strength and solubility. For radiopacity analysis, radiographs were made alongside an aluminum (Al) step wedge. To evaluate the antibacterial activity, direct contact test was performed on planktonic cells and Enterococcus faecalis biofilm induced on bovine root dentin for 14 days. The experimental periods were 5 and 15 h. Data were obtained as CFU mL-1. The obtained data were submitted to ANOVA and Tukey tests (p<0.05). The addition of AgNPs to WMTA increased the pH, lowered the solubility and the initial and final setting times. The addition of AgNPs to PC/ZrO2 maintained the pH, lowered the solubility, and increased the setting time and compressive strength. The radiopacity of all materials was higher than 4 mmAl. The addition of AgNPs promoted an increase in antibacterial activity for calcium silicate cements and favored the physicochemical and mechanical properties of the materials.

scholarly journals The Lyophilization Process Maintains the Chemical and Biological Characteristics of Royal Jelly

2015 ◽  
Vol 2015 ◽  
pp. 1-5 ◽  
Author(s):  
Andresa Piacezzi Nascimento ◽  
Larissa Ariana Roveroni Moraes ◽  
Nathália Ursoli Ferreira ◽  
Gabriela de Padua Moreno ◽  
Fernanda Grassi Mangolini Uahib ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Fatty Acid ◽  
Royal Jelly ◽  
Antibacterial Properties ◽  
Chemical Characterization ◽  
Resistant Bacteria ◽  
Bioactive Substances ◽  
Antibiotic Resistant ◽  
Chromatographic Profile ◽  
Main Fatty Acid

The alternative use of natural products, like royal jelly (RJ), may be an important tool for the treatment of infections caused by antibiotic-resistant bacteria. RJ presents a large number of bioactive substances, including antimicrobial compounds. In this study, we carried out the chemical characterization of fresh and lyophilized RJ and investigated their antibacterial effects with the purpose of evaluating if the lyophilization process maintains the chemical and antibacterial properties of RJ. Furthermore, we evaluated the antibacterial efficacy of the main fatty acid found in RJ, the 10-hydroxy-2-decenoic acid (10H2DA). Chromatographic profile of the RJ samples showed similar fingerprints and the presence of 10H2DA in both samples. Furthermore, fresh and lyophilized RJ were effective against all bacteria evaluated; that is, the lyophilization process maintains the antibacterial activity of RJ and the chemical field of 10H2DA. The fatty acid 10H2DA exhibited a good antibacterial activity againstStreptococcus pneumoniae. Therefore, it may be used as an alternative and complementary treatment for infections caused by antibiotic-resistantS. pneumoniae.

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