Inhibitory effect of Psidium guajava water extract in the development of 2,4-dinitrochlorobenzene-induced atopic dermatitis in NC/Nga mice

Abstract Background: Covid-19 Virus infection poses significant global health challenges and considered a global epidemic sweeping all countries of the world Which prompted scientists around the world to search for a quick or safe treatment to preserve people's lives .So far, options for controlling and treating the disease have not been revealed. The current study was conducted to evaluate the effectiveness of pomegranate peels extract against the Covid-19 virus in the laboratory. Methods: In this research, tow methods of extraction are carried out ethyl alcohol and distal water extract of pomegranate peels . activity of the extract assessed using 50% Tissue Culture Infectious Doses (TCID50) method in Vero E6 cells. Results: Pomegranate peels extract had the highest inhibitory effect against Covid -19 virus with IC50 value of 0.125, 0.0625 and 0.031256 μl in Vero E6 cells. Conclusion: Based on our results, the aqueous extract of pomegranate peels can inhibit Covid-19 virus replication in vitro.

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EFFICIENCY OF SOME PLANT EXTRACTS TO CONTROL OF PENICILLIUM ITALICUM WEHMER CAUSING BLUE MOLD ON LEMON FRUITS IN IRAQ

Pakistan Journal of Phytopathology ◽

10.33866/phytopathol.031.01.0472 ◽

2019 ◽

Vol 31 (1) ◽

pp. 01-05

Author(s):

Ahed A.H. Matloob ◽

Hamid A A. Khafaji

Keyword(s):

Plant Extracts ◽

Water Extract ◽

Inhibitory Effect ◽

Penicillium Italicum ◽

Blue Mold ◽

Lemon Fruit ◽

Water Extracts ◽

Wild Mustard ◽

First Time ◽

High Inhibitory Effect

The study aimed to evaluate the efficacy of the water extract of Propolis, Wild mustard and dates vinegar in the inhibition of Penicillium italicum causal agent of Blue mold on a lemon fruits. The results showed that all tested extracts with concentrations 5, 10 and 15% had a high inhibitory effect against P. italicum. Dates vinegar is highly effective and showed 100% inhibition of P. italicum and protected lemon fruit from infection. First time this type of natural inhibitory water extracts are used in Iraq which resulted that the dates vinegar is the best suitable option for the management of lemon blue mold disease in post-harvest.

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Inhibitory Effect of Angelica keiskei Extract in an Atopic Dermatitis Animal Model

Korean Journal of Food Preservation ◽

10.11002/kjfp.2012.19.5.792 ◽

2012 ◽

Vol 19 (5) ◽

pp. 792-798 ◽

Cited By ~ 9

Author(s):

Min-A Kim ◽

Hyeong-U Son ◽

Dong-Yoon Nam ◽

Yong-Su Cha ◽

Yong-Kyu Shin ◽

...

Keyword(s):

Animal Model ◽

Atopic Dermatitis ◽

Inhibitory Effect ◽

Angelica Keiskei

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Anti-Inflammatory Effect of Lycii radicis in LPS-Stimulated RAW 264.7 Macrophages

The American Journal of Chinese Medicine ◽

10.1142/s0192415x14500566 ◽

2014 ◽

Vol 42 (04) ◽

pp. 891-904 ◽

Cited By ~ 11

Author(s):

Mi Young Song ◽

Hyo Won Jung ◽

Seok Yong Kang ◽

Kyung-Ho Kim ◽

Yong-Ki Park

Keyword(s):

Inflammatory Cytokines ◽

Inflammatory Mediators ◽

Water Extract ◽

Inhibitory Effect ◽

Raw 264.7 Cells ◽

Raw 264.7 ◽

Root Bark ◽

Lycium Barbarum ◽

Anti Inflammatory ◽

Inflammatory Effect

The root bark of Lycium barbarum (Lycii radicis cortex, LRC) is used as a cooling agent for fever and night sweats in East Asian traditional medicine. The inhibitory effect of LRC water extract on inflammation is unknown. In this study, the anti-inflammatory effect of LRC was investigated in lipopolysaccharide (LPS)-stimulated mouse macrophage, RAW 264.7 cells. LRC extract significantly decreased the LPS-induced production of inflammatory mediators, nitric oxide (NO), prostaglandin (PG) E2 and pro-inflammatory cytokines, interleukin (IL)-1β and IL-6 in the cells. In addition, LRC extract inhibited the LPS-induced expression of inducible NO synthase (iNOS) and cyclooxygenase (COX)-2 mRNA and protein, and inflammatory cytokines mRNA in the cells. The action mechanism of LRC underlies the blocking of LPS-mediated p38 and Jun N-terminal kinase (JNK), mitogen-activated protein kinases (MAPKs), and the nuclear factor (NF)-κB signaling pathway. These results indicate that LRC extract inhibits the inflammatory response in activated macrophages by down-regulating the transcription levels of inflammatory mediators and blocking the MAPKs and NF-κB pathway.

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Inhibitory Effect of Opuntia humifusa Fruit Water Extract on Solar Ultraviolet-Induced MMP-1 Expression

International Journal of Molecular Sciences ◽

10.3390/ijms19092503 ◽

2018 ◽

Vol 19 (9) ◽

pp. 2503 ◽

Cited By ~ 4

Author(s):

Ah-Ram Han ◽

Tae-Gyu Lim ◽

Young-Ran Song ◽

Mi Jang ◽

Young Rhee ◽

...

Keyword(s):

Matrix Metalloproteinase ◽

Radical Scavenging ◽

Water Extract ◽

Inhibitory Effect ◽

Dermal Fibroblasts ◽

Ros Generation ◽

Skin Damage ◽

Opuntia Humifusa ◽

Matrix Metalloproteinase 1 ◽

Solar Ultraviolet

Opuntia humifusa is a type of cactus whose fruits have been used in folk medicine for the treatment of several diseases. In the present study, we aimed to determine whether O. humifusa fruit water extract (OHE) has inhibitory effects against solar ultraviolet (sUV)-induced matrix metalloproteinase-1 (MMP-1) expression. In ex vivo human skin, we found that OHE suppressed sUV radiation-induced MMP-1 expression. The inhibitory effect of OHE was confirmed in human dermal fibroblasts. OHE treatment reduced sUV-induced MMP-1 expression by suppressing reactive oxygen species (ROS) generation and phosphorylation of c-Jun, a component of transcription factor activator protein 1 (AP-1). On the other hand, OHE recovered the tissue inhibitor of matrix metalloproteinase 1 (TIMP-1) and type 1 collagen production attenuated by sUV. As upstream signaling pathways for AP-1, MKK4-JNK, MEK-ERK, and MKK3/6-p38 phosphorylation were downregulated by OHE treatment. In addition, OHE exhibited DPPH radical scavenging activity. These findings demonstrate that OHE has a preventive effect against sUV-induced skin damage via suppression of pathways triggered by ROS.

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Inhibitory Effect of Centella asiatica Extract on DNCB-Induced Atopic Dermatitis in HaCaT Cells and BALB/c Mice

Nutrients ◽

10.3390/nu12020411 ◽

2020 ◽

Vol 12 (2) ◽

pp. 411 ◽

Cited By ~ 4

Author(s):

Yonghyeon Lee ◽

Hyeon Kyeong Choi ◽

Kaudjhis Patrick Ulrich N’deh ◽

Young-Jin Choi ◽

Meiqi Fan ◽

...

Keyword(s):

Atopic Dermatitis ◽

Allergic Inflammation ◽

Centella Asiatica ◽

Ethanol Extract ◽

Inhibitory Effect ◽

Interferon Γ ◽

Dependent Manner ◽

Hacat Cells ◽

Signaling Mechanism ◽

Tnf Α

Atopic dermatitis (AD) is a chronic inflammatory skin disease caused mainly by immune dysregulation. This study explored the anti-inflammatory and immunomodulatory effects of the Centella asiatica ethanol extract (CA) on an AD-like dermal disorder. Treatment with CA inhibited the expression of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) in a dose-dependent manner in inflammatory stimulated HaCaT cells by interferon-γ (IFN-γ) and TNF-α-triggered inflammation. Eight-week-old BALB/c mice treated with 2,4-dinitrochlorobenzene (DNCB) were used as a mouse model of AD. In AD induce model, we had two types treatment of CA; skin local administration (80 µg/cm2, AD+CA-80) and oral administration (200 mg/kg/d, AD+CA-200). Interestingly, the CA-treated groups exhibited considerably decreased mast cell infiltration in the ear tissue. In addition, the expression of IL-6 in mast cells, as well as the expression of various pathogenic cytokines, such as TNF-α, IL-4, IL-5, IL-6, IL-10, IL-17, iNOS, COX-2, and CXCL9, was reduced in both AD+CA-80 and AD+CA-200 groups. Collectively, our data demonstrate the pharmacological role and signaling mechanism of CA in the regulation of allergic inflammation of the skin, which supports our hypothesis that CA could potentially be developed as a therapeutic agent for AD.

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