scholarly journals EFFICIENCY OF SOME PLANT EXTRACTS TO CONTROL OF PENICILLIUM ITALICUM WEHMER CAUSING BLUE MOLD ON LEMON FRUITS IN IRAQ

2019 ◽  
Vol 31 (1) ◽  
pp. 01-05
Author(s):  
Ahed A.H. Matloob ◽  
Hamid A A. Khafaji
Keyword(s):  
Plant Extracts ◽  
Water Extract ◽  
Inhibitory Effect ◽  
Penicillium Italicum ◽  
Blue Mold ◽  
Lemon Fruit ◽  
Water Extracts ◽  
Wild Mustard ◽  
First Time ◽  
High Inhibitory Effect

The study aimed to evaluate the efficacy of the water extract of Propolis, Wild mustard and dates vinegar in the inhibition of Penicillium italicum causal agent of Blue mold on a lemon fruits. The results showed that all tested extracts with concentrations 5, 10 and 15% had a high inhibitory effect against P. italicum. Dates vinegar is highly effective and showed 100% inhibition of P. italicum and protected lemon fruit from infection. First time this type of natural inhibitory water extracts are used in Iraq which resulted that the dates vinegar is the best suitable option for the management of lemon blue mold disease in post-harvest.

scholarly journals Effect ofNelumbo nuciferaPetal Extracts on Lipase, Adipogenesis, Adipolysis, and Central Receptors of Obesity

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Chandrasekaran Chinampudur Velusami ◽  
Amit Agarwal ◽  
Vijayalakshmi Mookambeswaran
Keyword(s):  
Medicinal Plants ◽  
Lipase Activity ◽  
Methanol Extract ◽  
Water Extract ◽  
Inhibitory Effect ◽  
Lipid Storage ◽  
Preclinical Models ◽  
Antagonist Activity ◽  
Water Extracts ◽  
Agonist And Antagonist

N. nuciferais one among the important medicinal plants assessed for its antiobesity action in various preclinical models. The present study was aimed at investigating the antiobesity effect of methanol and successive water extracts of petals ofN. nuciferaby studying its effect on adipogenesis, adipolysis, lipase, serotonin (5-HT2C), cannabinoid (CNR2), melanocyte concentrating hormone (MCHR1), and melanocortin (MC4R) receptors. Both methanol and successive water extracts ofN. nuciferapetals had an effect on inhibition of lipid storage in adipocytes and on increasing lipolysis.N. nuciferapetal methanol extract exhibited the concentration-dependent inhibitory effect on lipase activity with an IC50value of 47 µg/mL.N. nuciferapetal extracts showed evident agonist and antagonist activity towards 5-HT2Cand CNR2receptors, respectively, while it showed no effect towards MCHR1and MC4R receptors. Overall, methanol extract ofN. nuciferapetals showed better activity than successive water extract.

Germination and seedling emergence responses of common bean and cowpea to plant extract seed treatments

2016 ◽  
Vol 155 (1) ◽  
pp. 18-31 ◽  
Author(s):  
J. I. G. MASANGWA ◽  
Q. KRITZINGER ◽  
T. A. S. AVELING
Keyword(s):  
Seed Germination ◽  
Common Bean ◽  
Plant Extracts ◽  
Water Extract ◽  
Colletotrichum Lindemuthianum ◽  
Seed Treatments ◽  
Water Extracts ◽  
Cowpea Seeds ◽  
Cowpea Seed ◽  
Acetone Extracts

SUMMARYThe present study was initiated to investigate the effect of crude plant extracts as seed treatments on Phaseolus vulgaris (common bean) and Vigna unguiculata (cowpea) seed germination and emergence in the presence of Colletotrichum lindemuthianum and Colletotrichum dematium, respectively. Common bean and cowpea seeds were treated with crude water and acetone extracts of Agapanthus caulescens Spreng., Allium sativum L., Carica papaya L. and Syzygium cordatum Hochst.ex Krauss at 5 and 15 mg/ml concentrations. Seeds treated with the synthetic fungicide fludioxonil+mefenoxam (the commercial product Celest® XL) represented the positive control, whereas dimethyl sulphoxide and water-soaked seeds represented negative controls. The rolled paper towel method of the International Seed Testing Association was used to investigate the effect of the treatments on seed germination. Mean emergence time (MET) was determined using seed inoculated with the respective pathogens. The changes in the ultrastructure of embryonic roots and the connecting tissues of embryo-cotyledon of common bean and cowpea treated with Syzygium acetone extracts and Agapanthus water extracts were investigated using transmission electron microscopy (TEM). High germination percentages of >90% were observed in bean seeds from two production seasons treated with low concentrations of water extracts of Allium, Syzygium and Agapanthus and acetone extracts of Allium, Agapanthus and Carica. These treatments also recorded high emergence percentages with low MET values, which were similar to the water control. Cowpea seeds treated with Carica water extract had the highest germination and emergence. Syzygium acetone was the only extract that gave higher germination and emergence in both IT93K5132 and PAN 311 varieties. Therefore, Carica water and Syzygium acetone extracts can be considered as potential bean and cowpea seed treatments. Generally, there were inconsistencies in terms of correlations of germination with emergence percentages in both cowpea and bean seed treated with plant extracts used in the study, which could be due to differences in vigour. The TEM study of embryo-cotyledon tissue of both species revealed that Syzygium and Agapanthus extract seed treatment may accelerate metabolic processes as evidenced by the presence of vacuoles, many cristae and few lipid bodies.

scholarly journals Cholinesterase and microbial inhibitory activities of Tetrapleura tetraptera

2012 ◽  
Vol 4 (2) ◽  
pp. 156-163
Author(s):  
N. E. Okoronkwo ◽  
J. O. Echeme
Keyword(s):  
Inhibitory Activity ◽  
Water Extract ◽  
Antimicrobial Activities ◽  
Inhibitory Effect ◽  
Klebsiella Pneumonia ◽  
Inhibitory Effects ◽  
Continuous Increase ◽  
Water Extracts ◽  
Tetrapleura Tetraptera ◽  
Inhibitory Activities

The cholinesterase and microbial inhibitory activities of different parts of Tetrapleura tetraptera plant were evaluated due to their local applications. The cholinesterase results revealed that the extracts showed some levels of inhibitory effects depending on the solvents used. Tetrapleura tetraptera leaves had better inhibitory effects with maximum inhibitory activity of 70.0% at a concentration of 1.00mg/l for the water extract. Tetrapleura tetraptera bark showed highest inhibitory effect of 71.05% and (84.34%) for the ethanol and chloroform extracts at concentrations of 0.5mg/l and 1.0 mg/l respectively. While for petroleum ether, T. tetraptera bark recorded 74.34% inhibitory effect at concentration of 2.0 mg/l and also showed continuous increase in inhibitory activity as the concentration increases for aqueous methanol. The results of the antimicrobial activities showed that among all the test organisms, theethanol and water extracts of the leaves, stem, bark and root of the plants had promising activity against Escherichia coli, Staphylococcus aureus, Proteus mirabilis, Pseudomonas aeruginosa and Klebsiella pneumonia bacteria and Aspergillus fumigatus and Rhizopus species fungi. There was no activity shown by the ethanol and water extracts ofthe parts of the plants with Fugarium oxysporum, Penicillium chrysogenum and Mucor species fungi. The bacteria strains were more sensitive to the tested extracts than the fungi strains.

scholarly journals TECHNOLOGICAL AND ANALYTICAL STUDY OF THE WATER EXTRACTS FROM THE LEAVES OF LINGONBERRY

2017 ◽  
Vol 25 (2) ◽  
pp. 296-302
Author(s):  
N. G. Selezenev ◽  
A. N. Nikolashkin ◽  
S. V. Dobina
Keyword(s):  
Aqueous Extract ◽  
Analytical Study ◽  
Ph Value ◽  
Raw Materials ◽  
Water Extract ◽  
Water Extracts ◽  
Plant Raw Materials ◽  
Herbal Medicinal ◽  
Package Filter ◽  
First Time

In urological practice there is a sufficient demand for herbal medicinal preparations, of which the most widely used drugs are derived from arbutin-containing herbs. These medicines have diuretic, anti-inflammatory and antimicrobial activity. Currently, in connection with the decrease in number of production departments and pharmacies, water extraction can be made by the consumer at home from powdered medicinal herbs in filter bags and from packaged shredded medicinal plant raw materials in batches according to the instructions on the package. The object of the study was the aqueous extract obtained from the leaves of lingonberry. The article presents the study of manufacturing technology of water extracts from lingonberry leaves with the establishment of quality indicators in them. Investigated water extract obtained from raw materials packaged in bundles according to the instructions on the package, filter packages according to the instructions on the packaging of packaged raw materials in batches according to the method of State Pharmacopea XIII. The following were analyzed in the obtained water extracts: description, authenticity, dry residue, pH, content of phenolglucoside - arbutin. The research results were processed statistically. All of the studied aqueous extracts were transparent dark brown liquid that had a bitter, astringent taste characteristic of leaves of lingonberry. Dry residue in an aqueous extract, depending on the composition and technology was 0,65, 0,8% and of 1,93%. The content of arbutin was 0,43, 0,39 and 0,99%, pH of water extracts made from the crushed leaves of lingonberry, had close values of 5,03, 4,95 and, while in the extracts from powdered of lingonberry leaves in filter bags had pH value of 5,35, which may be attributed to the influence of the material of the filter bag. For the first time technologo-analytical study of the water extracts obtained at different technologies, established indicators of quality.

scholarly journals Brassinolide Enhances the Level of Brassinosteroids, Protein, Pigments, and Monosaccharides in Wolffia arrhiza Treated with Brassinazole

Plants ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1311
Author(s):  
Magdalena Chmur ◽  
Andrzej Bajguz
Keyword(s):  
Plant Growth ◽  
Growth And Development ◽  
Inhibitory Effect ◽  
Dependent Manner ◽  
Plant Growth And Development ◽  
Concentration Dependent Manner ◽  
Spectrophotometric Methods ◽  
Synthetic Inhibitor ◽  
Wolffia Arrhiza ◽  
First Time

Brassinolide (BL) represents brassinosteroids (BRs)—a group of phytohormones that are essential for plant growth and development. Brassinazole (Brz) is as a synthetic inhibitor of BRs’ biosynthesis. In the present study, the responses of Wolffia arrhiza to the treatment with BL, Brz, and the combination of BL with Brz were analyzed. The analysis of BRs and Brz was performed using LC-MS/MS. The photosynthetic pigments (chlorophylls, carotenes, and xanthophylls) levels were determined using HPLC, but protein and monosaccharides level using spectrophotometric methods. The obtained results indicated that BL and Brz influence W. arrhiza cultures in a concentration-dependent manner. The most stimulatory effects on the growth, level of BRs (BL, 24-epibrassinolide, 28-homobrassinolide, 28-norbrassinolide, catasterone, castasterone, 24-epicastasterone, typhasterol, and 6-deoxytyphasterol), and the content of pigments, protein, and monosaccharides, were observed in plants treated with 0.1 µM BL. Whereas the application of 1 µM and 10 µM Brz caused a significant decrease in duckweed weight and level of targeted compounds. Application of BL caused the mitigation of the Brz inhibitory effect and enhanced the BR level in duckweed treated with Brz. The level of BRs was reported for the first time in duckweed treated with BL and/or Brz.

scholarly journals Heterocyclic Aromatic Amines in Meat: Formation, Isolation, Risk Assessment and Inhibitory Effect of Plant Extracts

Foods ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1466
Author(s):  
Hafiz Rehan Nadeem ◽  
Saeed Akhtar ◽  
Tariq Ismail ◽  
Piero Sestili ◽  
Jose Manuel Lorenzo ◽  
...  
Keyword(s):  
Risk Assessment ◽  
Plant Extracts ◽  
Aromatic Amines ◽  
Extraction Procedure ◽  
Inhibitory Effect ◽  
Heating Time ◽  
Raw Material ◽  
Heterocyclic Aromatic Amines ◽  
Isolation And Identification ◽  
Cooked Meats

Heterocyclic aromatic amines (HAAs) are potent carcinogenic compounds induced by the Maillard reaction in well-done cooked meats. Free amino acids, protein, creatinine, reducing sugars and nucleosides are major precursors involved in the production of polar and non-polar HAAs. The variety and yield of HAAs are linked with various factors such as meat type, heating time and temperature, cooking method and equipment, fresh meat storage time, raw material and additives, precursor’s presence, water activity, and pH level. For the isolation and identification of HAAs, advanced chromatography and spectroscopy techniques have been employed. These potent mutagens are the etiology of several types of human cancers at the ng/g level and are 100- to 2000-fold stronger than that of aflatoxins and benzopyrene, respectively. This review summarizes previous studies on the formation and types of potent mutagenic and/or carcinogenic HAAs in cooked meats. Furthermore, occurrence, risk assessment, and factors affecting HAA formation are discussed in detail. Additionally, sample extraction procedure and quantification techniques to determine these compounds are analyzed and described. Finally, an overview is presented on the promising strategy to mitigate the risk of HAAs by natural compounds and the effect of plant extracts containing antioxidants to reduce or inhibit the formation of these carcinogenic substances in cooked meats.

scholarly journals A novel role for WAVE1 in controlling actin network growth rate and architecture

2015 ◽  
Vol 26 (3) ◽  
pp. 495-505 ◽  
Author(s):  
Meredith O. Sweeney ◽  
Agnieszka Collins ◽  
Shae B. Padrick ◽  
Bruce L. Goode
Keyword(s):  
Actin Filament ◽  
Specific Activity ◽  
Inhibitory Effect ◽  
Actin Network ◽  
Inhibitory Effects ◽  
Cellular Functions ◽  
Network Growth ◽  
Assembly Kinetics ◽  
Filament Networks ◽  
First Time

Branched actin filament networks in cells are assembled through the combined activities of Arp2/3 complex and different WASP/WAVE proteins. Here we used TIRF and electron microscopy to directly compare for the first time the assembly kinetics and architectures of actin filament networks produced by Arp2/3 complex and dimerized VCA regions of WAVE1, WAVE2, or N-WASP. WAVE1 produced strikingly different networks from WAVE2 or N-WASP, which comprised unexpectedly short filaments. Further analysis showed that the WAVE1-specific activity stemmed from an inhibitory effect on filament elongation both in the presence and absence of Arp2/3 complex, which was observed even at low stoichiometries of WAVE1 to actin monomers, precluding an effect from monomer sequestration. Using a series of VCA chimeras, we mapped the elongation inhibitory effects of WAVE1 to its WH2 (“V”) domain. Further, mutating a single conserved lysine residue potently disrupted WAVE1's inhibitory effects. Taken together, our results show that WAVE1 has unique activities independent of Arp2/3 complex that can govern both the growth rates and architectures of actin filament networks. Such activities may underlie previously observed differences between the cellular functions of WAVE1 and WAVE2.

scholarly journals Characterization of the inositol 1,4,5-trisphosphate-induced calcium release from permeabilized endocrine cells and its inhibition by decavanadate and p-hydroxymercuribenzoate

1989 ◽  
Vol 262 (1) ◽  
pp. 83-89 ◽  
Author(s):  
K J Föhr ◽  
J Scott ◽  
G Ahnert-Hilger ◽  
M Gratzl
Keyword(s):  
Endocrine Cells ◽  
Calcium Release ◽  
Cell Types ◽  
Inhibitory Effect ◽  
Maximal Effect ◽  
Thiol Compounds ◽  
Inositol 1,4,5 Trisphosphate ◽  
Dependent Inhibition ◽  
Pheochromocytoma Pc12 ◽  
First Time

The inositol 1,4,5-trisphosphate (IP3)-sensitive Ca2+ compartment of endocrine cells was studied with alpha-toxin- and digitonin-permeabilized rat insulinoma (RINA2) and rat pheochromocytoma (PC12) cells. The Ca2+ uptake was ATP-dependent, and submicromolar concentrations of IP3 specifically released the stored Ca2+. Half-maximal Ca2+ release was observed with 0.25-0.5 mumol of IP3/l, and the amount of Ca2+ released due to IP3 could be enhanced by additional loading of the Ca2+ compartment. Consecutive additions of the same concentration of IP3 for 1-2 h always released the same amount of Ca2+ without desensitization, providing an ideal basis to further characterize the IP3-induced Ca2+ release. Here we describe for the first time a reversible inhibitory effect of decavanadate on the IP3-induced Ca2+ release. Among the vanadium species tested (decavanadate, oligovanadate and monovanadate), only decavanadate was inhibitory, with a half-maximal effect at 5 mumol/l in both cell types. The effect of decavanadate could be overcome by increasing the amount of sequestered Ca2+ or added IP3. Decavanadate did not affect the ATP-driven Ca2+ uptake but oligovanadate was inhibitory on Ca2+ uptake. p-Hydroxymercuribenzoate (pHMB) at concentrations between 10 and 30 mumol/l also inhibited the Ca2+ release due to IP3. Thiol compounds such as dithiothreitol (DTT; 1 mmol/l) added before pHMB removed all its inhibitory effect on the IP3-induced Ca2+ release, whereas the inhibition caused by decavanadate was unaffected by DTT. Thus, the decavanadate-dependent inhibition functions by a distinctly different mechanism than pHMB and could serve as a specific tool to analyse various aspects of the IP3-induced Ca2+ release within endocrine cells.

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