scholarly journals Potential Effect of Pomegranate Peels Extract (Punica Granatum.) against Covid-19 Virus .

2021 ◽  
Author(s):  
Ashraf fawzy mosa ◽  
Mostafa abo Elhoda Mohamed
Keyword(s):  
Tissue Culture ◽  
Water Extract ◽  
Punica Granatum ◽  
Inhibitory Effect ◽  
Potential Effect ◽  
Laboratory Methods ◽  
Global Epidemic ◽  
Ic50 Value ◽  
The World

Abstract Background: Covid-19 Virus infection poses significant global health challenges and considered a global epidemic sweeping all countries of the world Which prompted scientists around the world to search for a quick or safe treatment to preserve people's lives .So far, options for controlling and treating the disease have not been revealed. The current study was conducted to evaluate the effectiveness of pomegranate peels extract against the Covid-19 virus in the laboratory. Methods: In this research, tow methods of extraction are carried out ethyl alcohol and distal water extract of pomegranate peels . activity of the extract assessed using 50% Tissue Culture Infectious Doses (TCID50) method in Vero E6 cells. Results: Pomegranate peels extract had the highest inhibitory effect against Covid -19 virus with IC50 value of 0.125, 0.0625 and 0.031256 μl in Vero E6 cells. Conclusion: Based on our results, the aqueous extract of pomegranate peels can inhibit Covid-19 virus replication in vitro.

scholarly journals The Effect ofHydrocotyle sibthorpioidesLam. Extracts onIn VitroDengue Replication

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Fitrien Husin ◽  
Yean Yean Chan ◽  
Siew Hua Gan ◽  
Siti Amrah Sulaiman ◽  
Rafidah Hanim Shueb
Keyword(s):  
Viral Replication ◽  
Methanolic Extract ◽  
Morphological Changes ◽  
Plaque Assay ◽  
Water Extract ◽  
Vero Cells ◽  
Inhibitory Effect ◽  
Potential Effect ◽  
Cell Viability Assay

Objective. To investigate the potential effect ofHydrocotyle sibthorpioidesLam. (H. sibthorpioides) extracts againstin vitrodengue viral replication.Methods. The cytotoxicity ofH. sibthorpioideswas evaluated using a cell viability assay. Cells were pre- and posttreated with water and methanol extracts ofH. sibthorpioides, and the viral inhibitory effect was investigated by observing the morphological changes, which were further confirmed by plaque assay.Results. The methanolic extract cytotoxicity was higher in Vero and C6/36 cells than the cytotoxicity of the water extract. Preincubation of the cells withH. sibthorpioidesextract showed nonexistent to mild prophylactic effects. The posttreatment of Vero cells withH. sibthorpioidesmethanolic extract presented higher antidengue activities when compared with the water extract. Surprisingly, posttreatment of C6/36 cells resulted in an enhancement of viral replication.Conclusion.H. sibthorpioideshad variable effects on dengue viral replication, depending on the treatment, cell lines, and solvent types. This study provides important novel insights on the phytomedicinal properties ofH. sibthorpioidesextracts on dengue virus.

scholarly journals Investigation of the effects of six medicinal plants with antiviral effects against COVID-19

2021 ◽  
Author(s):  
Harun ALP ◽  
Hasan ASİL ◽  
Demet Duman
Keyword(s):  
Medicinal Plants ◽  
Nigella Sativa ◽  
Water Extract ◽  
Antiviral Effect ◽  
Vero Cells ◽  
Glycyrrhiza Glabra ◽  
Crocus Sativus ◽  
Medicinal And Aromatic Plants ◽  
The World

Abstract Today, the coronavirus epidemic, which caused the death of 79 million cases and 1743 thousand people in 218 countries around the world, continues to increase its impact all over the world. Researchers are still trying to develop an effective solution against covid-19, including vaccines and drugs. However, there are few studies that determine the effect of natural products obtained from plants on covid-19. Medicinal and aromatic plants have been used for therapeutic purposes since the existence of humanity. In this study, the effects of some important medicinal plants including Licorice (Glycyrrhiza glabra), Saffron (Crocus sativus L.), Nigella (Nigella sativa L.), Laurel (Lauris nobilis), Karabaş (Lavandula stoechas), and Zahter (Thymbra spicata L. var. Spicata) against Covid-19 were investigated in vitro conditions. The six plants were evaluated for cytotoxic effect on Vero cells and determining inhibition of viral replication in Vero-E6 cells at concentrations of broad-spectrum antiviral non-cytotoxic against Covid-19 in cell culture and an additional antiviral effect against Covid-19. According to the results, the five examined plants (Saffron, Nigella, Laurel, Karabaş, Zahter) were ineffective against Covid-19 in vitro conditions. Interisingly, the water extract obtained from the root of the licorice plant (Glycyrrhiza glabra) inhibited Covid-19 in vitro conditions in the 2nd dilution (1: 4) following the initial concentration in Vero-E6 cells.

In Vitro and In Vivo Anti-Inflammatory Activity of the Rhizomes of Globba pendula Roxb

2021 ◽  
Vol 16 (10) ◽  
pp. 1934578X2110559
Author(s):  
Le Minh Ha ◽  
Ngo Thi Phuong ◽  
Nguyen Thi Thu Hien ◽  
Pham Thi Tam ◽  
Do Thi Thao ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Inhibitory Effect ◽  
Potential Effect ◽  
Inflammatory Activity ◽  
No Production ◽  
Anti Inflammatory ◽  
Inflammatory Effect

In this study, we aimed at evaluating in vitro and in vivo anti-inflammatory activity of various extracts of the rhizomes of Globba pendula Roxb. Three extracts ( n-hexane, ethyl acetate, and water) were screened for their inhibitory effect on NO production by lipopolysaccharide-stimulated RAW 264.7 macrophages. The ethyl acetate extract of G. pendula rhizomes (EGP) showed a potential effect with an IC50 value of 32.45 µg/mL. For in vivo study, the ethyl acetate extract was further investigated for its anti-inflammatory effect using collagen antibody-induced arthritic mice (CAIA). The level of arthritis in experimental mice significantly reduced ( P < .05) after treatment with EGP at a dose of 500 mg/kg body weight (b.w.). This study also revealed that EGP is orally non-toxic. Ethyl p-methoxy cinamate was identified as the main constituent of EGP, which may result in its anti-inflammatory effect.

scholarly journals Biological and Computational Studies for Dual Cholinesterases Inhibitory Effect of Zerumbone

Nutrients ◽  
2020 ◽  
Vol 12 (5) ◽  
pp. 1215 ◽  
Author(s):  
Jayeong Hwang ◽  
Kumju Youn ◽  
Yeongseon Ji ◽  
Seonah Lee ◽  
Gyutae Lim ◽  
...  
Keyword(s):  
Docking Simulation ◽  
Inhibitory Effect ◽  
Potential Effect ◽  
Van Der Waals Interactions ◽  
Inhibitory Effects ◽  
Computational Docking ◽  
In Silico Docking ◽  
Ic50 Values ◽  
Physiological Benefits

Acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) mediate the degradation of acetylcholine (ACh), a primary neurotransmitter in the brain. Cholinergic deficiency occurs during the progression of Alzheimer’s disease (AD), resulting in widespread cognitive dysfunction and decline. We evaluated the potential effect of a natural cholinesterase inhibitor, zerumbone, using in vitro target enzyme assays, as well as in silico docking and ADMET (absorption, distribution, metabolism, excretion, and toxicity) simulation. Zerumbone showed a predominant cholinesterase inhibitory property with IC50 values of 2.74 ± 0.48 µM and 4.12 ± 0.42 µM for AChE and BChE, respectively; however, the modes of inhibition were different. Computational docking simulation indicated that Van der Waals interactions between zerumbone and both the cholinesterases were the main forces responsible for its inhibitory effects. Furthermore, zerumbone showed the best physicochemical properties for both bioavailability and blood–brain barrier (BBB) permeability. Together, in the present study, zerumbone was clearly identified as a unique dual AChE and BChE inhibitor with high permeability across the BBB, suggesting a strong potential for its physiological benefits and/or pharmacological efficacy in the prevention of AD.

scholarly journals Screening and Application of Chitin Synthase Inhibitors

Processes ◽  
2020 ◽  
Vol 8 (9) ◽  
pp. 1029
Author(s):  
Xiaozai Shi ◽  
Shuo Qiu ◽  
Yingling Bao ◽  
Hanchi Chen ◽  
Yuele Lu ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Inhibitory Concentration ◽  
Inhibitory Effect ◽  
Chitin Synthase ◽  
Fungal Cell ◽  
Ic50 Value ◽  
Further Development ◽  
Fungicide Target ◽  
Better Than

Chitin is an important part of the fungal cell wall, but is not found in plants and mammals, so chitin synthase (CHS) can be a green fungicide target. In this paper, 35 maleimide compounds were designed and synthesized as CHS inhibitors. All the screened compounds showed different degrees of CHS inhibitory activity and antifungal activity in vitro. In particular, the half–inhibitory concentration (IC50) value of compound 20 on CHS was 0.12 mM, and the inhibitory effect was better than that of the control polyoxin B (IC50 = 0.19 mM). At the same time, this compound also showed good antifungal activity and has further development value.

scholarly journals IN VITRO ANTI-OBESITY EFFECT OF MACROLICHENS HETERODERMIA LEUCOMELOS AND RAMALINA CELASTRI BY PANCREATIC LIPASE INHIBITORY ASSAY

2017 ◽  
Vol 9 (5) ◽  
pp. 137
Author(s):  
Rashmi Shivanna ◽  
Hengameh Parizadeh ◽  
Rajkumar H. Garampalli
Keyword(s):  
Ethyl Acetate ◽  
Pancreatic Lipase ◽  
Methanol Extract ◽  
Well Being ◽  
Inhibitory Effect ◽  
Potential Effect ◽  
Protective Role ◽  
Treatment Of Obesity ◽  
Molecular Sequencing

Objective: Obesity is a chronic disorder caused by an imbalance between energy intake and expenditure in which excessive fat will be deposited in adipose tissue and poses a risk to the health and well-being of humans. Agents which inhibit pancreatic lipase play an important role in the treatment of obesity. The aim of this study was to assess the potential effect of macro lichens Heterodermia leucomelos (L.) Poelt a foliose lichen and Ramalina celastri (Sprengel) Krog and Swinscow a fruticose lichen in the treatment of obesity.Methods: In vitro anti-obesity inhibitory effect of macro lichens were evaluated by using chicken pancreatic lipase activity. Lipase was extracted from the chicken pancreas. Different concentrations from 5-25 mg/ml of methanol and ethyl acetate extracts of lichens Heterodermia leucomelos and Ramalina celastri was incubated with pancreas lipase.Results: With the increase in the concentration of extracts the higher inhibition of the enzyme was observed. Solvent methanol showed good activity compared to ethyl acetate. Percentage of inhibition ranged from 19.7-69.8 and 20.0-86.6 % in the methanol extract of Heterodermia leucomelos and Ramalina celastri respectively. Comparatively lichen Ramalina celastri in methanol extract showed maximum inhibition of 86.6 %, whereas ethyl acetate showed an inhibition of 63.0% at 25 mg/ml against enzyme lipase.Conclusion: In the present study, the inhibitory activity of lichen indicates its protective role in treating obesity. Molecular sequencing of this lichen helps in future to determine the various metabolic pathways that are responsible for the production of novel compounds.

scholarly journals Tyrosinase Inhibitory Effect, Antioxidant and Anticancer Activities of Bioactive Compounds in Ripe Hog Plum (Spondias Pinnata) Fruit Extracts

2019 ◽  
Vol 35 (3) ◽  
pp. 916-926
Author(s):  
Supawadee Patathananone ◽  
Jureerut Daduang ◽  
Amonrat Koraneekij ◽  
Chia-Ying Li
Keyword(s):  
High Performance ◽  
Antioxidant Property ◽  
Inhibitory Effect ◽  
Tyrosinase Activity ◽  
Normal Fibroblast ◽  
Anticancer Activities ◽  
Ic50 Value ◽  
Bioactive Agents ◽  
Hog Plum

The usage of ripe hog plum fruit (Spondias pinnata) extracts in cosmetics and food products, including cancer therapeutic agents, have a few studies. Herein, the strong anti-tyrosinase activity found in the extracted part of isopropanol is reported. This extract was separated by liquid/liquid extraction using hexane: methanol+H2O. The hydrophilic layer (6A*) exhibited the anti-tyrosinase, antioxidant, and anticancer activities in vitro. The IC50 value of each bioactivity was presented as approximately 0.18, 0.04, and 1.40 mg/ml, respectively. In addition, 6A* fraction showed a very low cytotoxic effect in normal fibroblast cells (NHDF cells). The bioactive agents in 6A* were purified by C18 reverse-phase High-Performance Liquid Column Chromatography (HPLC). The 12 purified peaks were shown in the chromatogram profile. All peaks (excepted 6A-06 and 6A-09) displayed anti-tyrosinase activity, whereas the antioxidant property was not found in 6A-01, 6A-06, and 6A-08 but was represented in other peaks. Most purified peaks were indicated to be the aromatic alcohol or derivative phenol compounds.

scholarly journals The local anaesthetic procaine prodrugs ProcCluster® and Procaine-hydrochloride impair SARS-CoV-2 replication in vitro

2021 ◽  
Author(s):  
Clio Häring ◽  
Josefine Schroeder ◽  
Bettina Loeffler ◽  
Beatrice Engert ◽  
Christina Ehrhardt
Keyword(s):  
Local Anaesthetic ◽  
Influenza A ◽  
Antiviral Treatment ◽  
Treatment Strategies ◽  
Inhibitory Effect ◽  
Procaine Hydrochloride ◽  
Influenza A Viruses ◽  
Cell Culture Systems ◽  
The World

The SARS-CoV-2 pandemic has had the world in suspense for more than a year. Even if more and more vaccines are approved there is still an urgent need for efficient antiviral treatment strategies. Here, we present data on the inhibitory effect of the local anaesthetic procaine, especially the prodrugs ProcCluster® and Procaine-hydrochloride on SARS-CoV-2 infection in vitro. Remarkably, similar effects could be shown on the replication of influenza A viruses in cell culture systems. Since the active ingredient procaine is well-tolerated and already used in the clinics for anaesthetic purposes, the further investigation of this substance could enable its reuse in antiviral therapy, including SARS-CoV-2.

scholarly journals Kajian Penambahan BahanOrganik Pada Media Tanam VW Pada Organogenesis AnggrekDendrobium SecaraIn Vitro

2018 ◽  
Vol 1 (1) ◽  
Author(s):  
Siti Rahmah ◽  
Tintrim Rahayu ◽  
Ari Hayati
Keyword(s):  
Tissue Culture ◽  
Organic Matter ◽  
Optimization Technique ◽  
Media Composition ◽  
Growth Optimization ◽  
The World ◽  
The Media ◽  
Number Of Leaves ◽  
Bean Sprouts

In vitro tissue culture is a growth optimization technique of Dendrobium orchid with according to media composition. Nutritions in the media are important for dendrobium orchid. Dendrobium orchid  include plant from orchidaceae family its spread throughout the world like indonesia. Its features are easily planted, intersest is continuous and varied, easily assembled, the flower crown is not easy to fall and wither. Research aimed at obtaining media compositions that are easily available and able to fulfill the needs of orchid plants. The research was conducted using descriptive methods to compare different trearment; Vacin & Went and VW media with adding organic matter; extract bean sprouts, potato extrac, and water coconut; wich is conducted for eight weeks after planting. The result of addition organic matter on VW media was different toward organogenesis of orchid. The average number of shoots is 1.8; the number of leaves average of 6.8 and the number of roots average of 3.6 formed from two until eight weeks after culture.Keywords: tissue culture, growing media, Dendrobium orchid, organogenesis.ABSTRAKKultur jaringan in vitro adalah salah satu teknik optimalisasi pada pertumbuhan tanaman angrek Dendrobium dengan menyesuaikan komposisi medianya. Nutrisi yang terdapat di dalam media sangat penting bagi pertumbuhan anggrek. Anggrek Dendrobium termasuk tanaman dari keluarga Orchidaceae yang penyebarannya sampai ke pelosok dunia seperti Indonesia. Keistimewaanya mudah ditanam, bunganya terus-menerus dan bermacam-macam, mudah disusun, serta mahkota bunga tidak mudah jatuh dan layu. Penelitian yang bertujuan untuk mendapatkan komposisi media yang mudah didapat dan mampu memenuhi kebutuhan tanaman anggrek. Penelitian dilakukan menggunakan metode deskriptif untuk membandingkan perlakuan media yang berbeda yaitu media Vacin & Went, dan VW dengan penambahan bahan organik; ekstrak tauge kacang hijau, ekstrak kentang, dan air kelapa muda; yang dilakukan selama delapan minggu setelah tanam. Hasil penambahan bahan organik pada media VW berbeda terhadap organogenesis eksplan anggrek. Jumlah tunas rata-rata 1,8; Jumlah daun rata-rata 6,8 dan jumlah akar rata-rata 3,6 yang terbentuk dari dua minggu setelah kultur (MSK) sampai minggu terakhir pengamatan delapan MSK.Kata kunci: kultur jaringan, media tanam, angrek Dendrobium,organogenesis.

scholarly journals An in-vitro study to compare and evaluate the anti-diabetic effect of mixed fruit juice nanoemulsion with acarbose

2021 ◽  
Vol 12 (1) ◽  
pp. 72-76
Author(s):  
Gayathri K ◽  
Karpagavel L ◽  
Murugavel K ◽  
Manikandan N
Keyword(s):  
Fruit Juice ◽  
In Vitro Study ◽  
Punica Granatum ◽  
Inhibitory Effect ◽  
Health Concern ◽  
Maximum Inhibition ◽  
Nano Emulsion ◽  
Alpha Glucosidase ◽  
Relative Inhibition

Diabetes has become a global health concern, its management lies hugely on expensive medical care and monitoring; thus the need to investigate possible alternatives to reduce treatment cost and also to reduce the side effects of commonly used anti diabetic drugs. This study aims to assess the anti-diabetic effect of mixed fruit juice nanoemulsion of  Coccinia grandis, Punica granatum and Phyllanthus emblica. Mixed fruit juice nanoemulsion were prepared in the ratio of VCO oil:water:surfactant- 32:36:32 (%w/w). characterization parameters of mixed fruit juice nanoemulsion including PH, zeta potential, size, morphology were within the standard limits and was used for the study. The anti diabetic effect of nano emulsified mixed fruit juice was compared with that of acarbose by measuring their inhibitory effect on the enzyme alpha glucosidase. Maximum Inhibitory effect of formulated Nano emulsion on enzyme alpha glucosidase was 83% at concentration 81μg/ml whereas that for the standard anti-diabetic drug Acarbose was 93% at concentration 540μg/ml. For acarbose, the maximum Inhibition was 92% at the concentration of 540μg/ml. The relative inhibition Percentage of Nano emulsion versus Control has been analyzed and the results found to be statistically significant (P<0.003).

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