Ananas comosus (L) Merrill (pineapple) fruit peel extract demonstrates antimalarial, anti-nociceptive and anti-inflammatory activities in experimental models

Bromelain is a complex combination of multiple endopeptidases of thiol and other compounds derived from the pineapple fruit, stem and/or root. Fruit bromelain and stem bromelain are produced completely distinctly and comprise unique compounds of enzymes, and the descriptor “Bromelain” originally referred in actuality to stem bromelain. Due to the efficacy of oral administration in the body, as a safe phytotherapeutic medication, bromelain was commonly suited for patients due to lack of compromise in its peptidase efficacy and the absence of undesired side effects. Various in vivo and in vitro studies have shown that they are anti-edematous, anti-inflammatory, anti-cancerous, anti-thrombotic, fibrinolytic, and facilitate the death of apoptotic cells. The pharmacological properties of bromelain are, in part, related to its arachidonate cascade modulation, inhibition of platelet aggregation, such as interference with malignant cell growth; anti-inflammatory action; fibrinolytic activity; skin debridement properties, and reduction of the severe effects of SARS-Cov-2. In this paper, we concentrated primarily on the potential of bromelain’s important characteristics and meditative and therapeutic effects, along with the possible mechanism of action.

Download Full-text

Antibacterial Activity Test of Ethanol Extract Pineapple (Ananas comosus (L.) Merr.) Peel against Growth of Propionibacterium acnes

Borneo Journal of Pharmacy ◽

10.33084/bjop.v2i2.928 ◽

2019 ◽

Vol 2 (2) ◽

pp. 108-113

Author(s):

Fitriyanti Fitriyanti ◽

Muhammad Nur Rahman Hendrawan ◽

Karunita Ika Astuti

Keyword(s):

Antibacterial Activity ◽

Propionibacterium Acnes ◽

Ethanol Extract ◽

Inhibition Zone ◽

Negative Control ◽

Ananas Comosus ◽

Treatment Groups ◽

Pineapple Peel ◽

Pineapple Fruit ◽

Peel Extract

Pineapple peel (Ananas comosus (L.) Merr.) is waste from the pineapple fruit. The purpose of this study was to determine the content of pineapple peel extract and to antibacterial activity in various concentrations effective in inhibiting the growth of Propionibacterium acnes. The pineapple peel extract is made by using the extraction method in the form of maceration. The method used in the inhibitory test using the three replication samples in each treatment group. The sample consisted of 10 treatment groups ie pineapple peel extract concentration 12.5%, 25%, 37.5%, 50%, 62.5%, 75%, 87.5%, and 100%, as well as positive and negative control. The results obtained from pineapple peel screening contain flavonoid and saponin compounds. The data analysis shows that pineapple peel extract concentrations of 50%, 62.5%, 75%, 87.5%, and 100% had inhibitory zones with the medium-strong category while clindamycin as the control has a strong inhibition zone. The conclusion of this research proves that pineapple peel extract has antibacterial power to P. acnes with effective concentration is 100% with the strong category.

Download Full-text

Gambaran Hasil Aktivitas Antioksidan Berberapa Perlakuan Teknik Preparasi Pembuatan Konsentrasi Ekstrak Kulit Buah Nanas (Ananas comosus L.Merr) Menggunakan Senyawa DPPH (1,1-diphenyl-2-picrylhydrazyl)

Proceeding of Mulawarman Pharmaceuticals Conferences ◽

10.25026/mpc.v10i1.365 ◽

2019 ◽

Vol 10 ◽

pp. 44-47

Author(s):

Syarifah maryam Alaydrus ◽

Wahyu Widayat ◽

Laode Rijai

Keyword(s):

Antioxidant Activity ◽

Volume Ratio ◽

Ananas Comosus ◽

Preparation Process ◽

Extract Concentration ◽

Fruit Skin ◽

Pineapple Peel ◽

Pineapple Fruit ◽

Preparation Techniques ◽

Peel Extract

Pineapple skin (Ananas comosus L.Merr) has been known to have a category of strong antioxidant activity against reducing DPPH compounds (1,1-diphenyl-2-picrylhydrazyl). However, references to the preparation process of extract concentration making and the treatment of DPPH compounds to extracts are not much elaborated, so that it can cause errors in inferring the category of antioxidant activity. The purpose of this study was to determine the results of antioxidant activity through the treatment of preparation techniques for the concentration of pineapple peel extract concentration. Pineapple peel extract (Ananas comosus L. Merr) was prepared by maceration method using 70% ethanol solvent. Testing of antioxidant activity was carried out using the UV-Vis spectrophotometric method at a wavelength of 516.4 nm. The extract concentration to be tested was 500 μg/mL, 250 μg/mL, 125 μg/mL, 62.5 μg/mL and 31.25 μg/mL and the concentration of DPPH compounds (1,1-diphenyl-2-picrylhydrazyl) was 45 μg/mL. Treat the extract making concentration divided into 3 namely P1 (multilevel dilution with a volume ratio of 1: 1) P2 (normal dilution with a volume ratio of 1: 1) and P3 (DPPH solution is used to suffice the volume of the extracted test extract). The results of percent inhibitors from each treatment of making concentrations showed P1 <P2, P1 <P3, P2 <P3, and IC50 of each treatment were P1 268.07 μg/mL, P2 was 231.28 μg/mL, P3 was 78.403 μg/mL. Based on the results of IC50 P3 gives the category of antioxidant activity that is 78,403.Keywords: pineapple fruit skin (Ananas comosus L. Merr), antioxidants, dpph (1,1-diphenyl-2- picrylhydrazyl), dilution.

Download Full-text

Passion fruit peel extract attenuates bleomycin-induced pulmonary fibrosis in mice

Canadian Journal of Physiology and Pharmacology ◽

10.1139/cjpp-2014-0006 ◽

2014 ◽

Vol 92 (8) ◽

pp. 631-639 ◽

Cited By ~ 7

Author(s):

Shanmuga Reddy Chilakapati ◽

Mamatha Serasanambati ◽

Pavan Kumar Manikonda ◽

Damodar Reddy Chilakapati ◽

Ronald Ross Watson

Keyword(s):

Pulmonary Fibrosis ◽

Antioxidant Activities ◽

Intratracheal Instillation ◽

Inflammatory Cells ◽

Lavage Fluid ◽

Passion Fruit ◽

Fruit Peel ◽

Tissue Sections ◽

Anti Inflammatory ◽

Peel Extract

Idiopathic pulmonary fibrosis is a progressive fatal lung disease characterized by excessive collagen deposition, with no effective treatments. We investigated the efficacy of natural products with high anti-inflammatory activity, such as passion fruit peel extract (PFPE), in a mouse model of bleomycin-induced pulmonary fibrosis (PF). C57BL/6J mice were subjected to a single intratracheal instillation of bleomycin to induce PF. Daily PFPE treatment significantly reduced loss of body mass and mortality rate in mice compared with those treated with bleomycin. While bleomycin-induced PF resulted in elevated total numbers of inflammatory cells, macrophages, lymphocytes, and neutrophils in bronchoalveolar lavage fluid on both days 7 and 21, PFPE administration significantly attenuated these phenomena compared with bleomycin group. On day 7, the decreased superoxide dismutase and myeloperoxidase activities observed in the bleomycin group were significantly restored with PFPE treatment. On day 21, enhanced hydroxyproline deposition in the bleomycin group was also suppressed by PFPE administration. PFPE treatment significantly attenuated extensive inflammatory cell infiltration and accumulation of collagen in lung tissue sections of bleomycin-induced mice on days 7 and 21, respectively. Our results indicate that administration of PFPE decreased bleomycin-induced PF because of anti-inflammatory and antioxidant activities.

Download Full-text

KARAKTERISTIK FISIKOKIMIA SELAI KULIT BUAH NAGA (Hylocereus polyrhizus) DENGAN PENAMBAHAN VARIASI KONSENTRASI BUAH NANAS (Ananas comosus L.)

JURNAL AGROTEKNOLOGI ◽

10.19184/j-agt.v15i01.20644 ◽

2021 ◽

Vol 15 (01) ◽

pp. 59

Author(s):

Pratiwi Jati Palupi ◽

Retno Prasetia ◽

Muh. Doddy Pratama ◽

Indah Sriwahyuni

Keyword(s):

Moisture Content ◽

Ananas Comosus ◽

Chemical Characteristics ◽

Observation Data ◽

Fruit Peel ◽

Dragon Fruit ◽

Significant Difference ◽

Pineapple Fruit ◽

Hylocereus Polyrhizus ◽

Color Texture

Dragon fruit peels (Hylocereus polyrhizus) are generally only considered trash by the public because they are considered useless, even though dragon fruit peels still contain useful nutrients. Dragon fruit skin can be used as food, one of which is a jam. The addition of pineapple fruit variations to dragon fruit peel jam as a flavor enhancer and natural fragrance to reduce the use of sugar in jam and the aroma of pineapple fruit can mask the distinctive aroma of dragon fruit which is less desirable. The purpose of this study was to determine the appropriate concentration variations in the manufacture of dragon fruit peel jam with the addition of pineapple, which consumers liked. This study used a completely randomized design with six (6) treatments and two (2) replications. Dragon fruit peel jams analyzed by chemical tests included moisture content, sugar level, and pH, while organoleptic test included color, texture, taste, aroma, and a total of 30 untrained panelists. Observation data were processed using ANOVA (α = 5%) and further tested using the honest significant difference test (BNJ) (α = 5%). The results showed that the pineapple’s addition to dragon fruit peel jam had a very significant effect on the panelists' preference for aroma and taste, but not significantly different from the color, texture, and chemical characteristics of jam. The N6 treatment (dragon fruit peel 250 g: pineapple fruit 250 g) was the best jam, based on chemical characteristics (pH content of 3.52, moisture content of 18.65%, sugar level of 53.30%) and preferable in organoleptic characteristics (2.97 of color, 2.97 of texture, 2.97 of flavor, 3.00 of taste). Keywords: agricultural waste, dragon fruit peel, jam, pineapple

Download Full-text

Prophylactic Effect of Opuntia ficus indica Fruit Peel Extract against Irradiation-Induced Colon Injury in Rats

Planta Medica ◽

10.1055/a-1019-9801 ◽

2019 ◽

Vol 86 (01) ◽

pp. 61-69 ◽

Cited By ~ 1

Author(s):

Salma A. Elsawi ◽

Rasha R. Radwan ◽

Marwa M. Elbatanony ◽

Amal M. El-Feky ◽

N. H. Sherif

Keyword(s):

Histopathological Examination ◽

Petroleum Ether Extract ◽

Intercellular Adhesion Molecule ◽

Unsaponifiable Fraction ◽

Prophylactic Effect ◽

Colon Injury ◽

Fruit Peel ◽

Opuntia Ficus Indica ◽

Anti Inflammatory ◽

Peel Extract

Abstract Opuntia ficus-indica extract has been used in traditional folk medicine for several purposes and exhibits anti-inflammatory properties. This study was directed to explore the prophylactic effect of O. ficus-indica fruit peel extract against irradiation-induced colitis in rats. GC/MS analysis of the petroleum ether extract led to recognition of 33 compounds in the unsaponifiable fraction and 15 fatty acid methyl esters in the saponifiable part. Thirteen terpenes and sterols were isolated and identified from which ten compounds were not isolated from any part of this species before. Data showed that irradiation induced colon injury as manifested by elevated contents of malondialdehyde, nitric oxide, myeloperoxidase, intercellular adhesion molecule-1, cyclooxygenase-2, tumor necrosis factor alpha, and nuclear factor kappa B, while it reduced superoxide dismutase activity and interleukin 10 content in colonic tissues, which was confirmed by histopathological examination. Pretreatment with O. ficus-indica extract attenuated the alteration in the measured parameters. It could be concluded that O. ficus-indica fruit peel extract can be regarded as a potential agent in limiting colonic complications due to irradiation, possibly by its antioxidant and anti-inflammatory properties.

Download Full-text

Characterization and Potential Application of Bromelain from Pineapple (Ananas comosus) Waste (Peel) in Recovery of Silver from X-Ray Films

International Journal of Biomaterials ◽

10.1155/2021/9964337 ◽

2021 ◽

Vol 2021 ◽

pp. 1-12

Author(s):

Muhammed Seid Anbesaw

Keyword(s):

Phosphate Buffer ◽

Potential Application ◽

Industrial Applications ◽

Significant Loss ◽

Ananas Comosus ◽

Fruit Peel ◽

Silver Recovery ◽

Minimum Concentration ◽

X Ray ◽

Pineapple Fruit

Bromelain is a proteolytic enzyme, which is predominately found in all parts of a pineapple plant (Ananas comosus). It has immense application in the pharmaceutical industry as well as in food, cosmetic, and leather industries. However, bromelain from pineapple fruit peels is a less explored source for making valuable products. Therefore, the objective of this study was to characterize and investigate the potential application of bromelain enzyme extracted from pineapple juice processing waste peels in gelatin hydrolysis and removal of silver from X-ray films. Extraction of bromelain was performed with a 1 : 2 ratio (w/v) of the extraction mix, pineapple fruit peel, in phosphate buffer (pH = 7.0). The activity of a crude enzyme was 7.2 U/ml, and it was active in a broad range of pH (2.5–12) and temperature (25–85°C) without losing its activity. This implies that the enzyme is heat tolerant. The optimum temperature and pH of the enzyme were recorded at 70°C and pH 7.0, respectively. At optimum conditions (70°C and pH 7.0), complete hydrolysis of the gelatin layer from X-ray film was observed at 30 and 34 seconds, respectively. The enzyme was repeatedly used more than 50 times without significant loss of its activity. Using a minimum concentration of bromelain (3 ml = 21.6 U) along with phosphate buffer (37 ml), it is possible completely to remove gelatin within 210 seconds. The properties of the enzyme showed that it has promising potential industrial applications for repeated utilization of the enzyme in both silver recovery and recycling of the X-ray film base.

Download Full-text

The Utilization of Red Dragon Fruit Peel Extract (Hylocereus costaricencis) As A Natural Colorant of Dried Noodle

Serambi Journal of Agricultural Technology ◽

10.32672/sjat.v2i1.2037 ◽

2020 ◽

Vol 2 (1) ◽

Author(s):

Silaturahmi Silaturahmi ◽

Zaidiyah Zaidiyah ◽

Tengku Mia Rahmiati

Keyword(s):

Product Diversification ◽

Fruit Peel ◽

Physical Quality ◽

Natural Colorant ◽

Randomized Design ◽

Solid Loss ◽

Dragon Fruit ◽

Completely Randomized Design ◽

Peel Extract

The use of colorants in the dried noodle manufactures is an effort of product diversification. Besides of being used as a natural coloring agent, this peel extract is also used to improve nutritional value of the expected product. The purpose of this study was to determine the effect of red dragon fruit peel extract on the physical quality of dried noodle. The study was conducted by using Completely Randomized Design (CRD) method with one factor, namely concentration of red dragon fruit peel extract (N1 = 10 ml, N2 = 15 ml, N3 = 20 ml, N4 = 25 ml, N5 = 30 ml). The observations of its physical quality consisted of water absorption, solid loss during cooking, and organoleptic tests (aroma and color). The best quality of dried noodle was obtained by using 30 ml red dragon fruit peel extract (N5) with physical properties, namely DSA levels of 351.92%, solid loss during cooking 4.78%, aroma 3.79 (like) and color 3.89 (like).

Download Full-text